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lectin binding    
相关语句
  凝集素
    Lectin Binding Sites in Normal Mouse Testis and Epididymis
    正常小鼠睾丸及附睾的凝集素结合部位
    Four lectins (WGA. UEA, LCA and BSA) were used study the distribution of lectin binding in the esophageal epithelium of human embryos.
    选用四种凝集素(WGA、UEA、LCA、BSA)对人胚食管采用ABC法进行石蜡切片的标记.
短句来源
    Biotinylated lectins:UEA Ⅰ, RCA Ⅰ, DBA, PSA, PNA, BSL, LCA, WGA, ConA AND SBA were used as probes to identify lectin binding sites and to determine if lectin binding patterns change with age in the developing of human fetal esophageal epithelium (8W, 14W, 20W, 28W and 32W). Lectin binding was visualized in fomalin fixed and avidin peroxidase staining procedures.
    采用十种生物素化凝集素 U EA- I、 DBA、 PSA、 BSL、 PNA、 L CA、 RCA- I、SBA、 Con A及 WGA分别对 8W、1 4 W、 2 0 W  2 8W及 32 W的人胎儿食管上皮进行研究 ,以确定胎儿食管上皮在发育过程中凝集素的结合位点以及结合方式随年龄的变化。
短句来源
    It is suggested that the distribution of lectin binding and staining inten-sity might serve as a marker of development and differentiation of esophageal epithelium.
    本研究结果提示,上述凝集素在人胚食管上皮的结合部位的分布情况及染色强度,可作为该上皮细胞的发育、分化程度的标志之一.
短句来源
  凝集素结合
    Lectin Binding Sites in Normal Mouse Testis and Epididymis
    正常小鼠睾丸及附睾的凝集素结合部位
    Observation of Lectin Binding Stites in Esophageal Epithelium of Human Embryos
    人胚食管上皮凝集素结合部位的观察
短句来源
    THE STUDY ON LECTIN BINDING SITES IN THE MUCOSAL EPITHELIUM OF MUMAN FETAL INTESTINE
    人胚胎肠道粘膜凝集素结合部位的研究
短句来源
    Study of Characterization of Lectin Binding Endometrium of Repeated Spontaneous Abortion(rsa) in Secretory Phase
    分泌期反复流产子宫内膜凝集素结合特性探讨
短句来源
  凝集素标记
    EVALUATION OF LECTIN BINDING TO HUMAN FEMALE GENITAL ORGANS AND PLACENTA
    人女性生殖器官和胎盘组织的凝集素标记研究
短句来源
  凝集素标记
    EVALUATION OF LECTIN BINDING TO HUMAN FEMALE GENITAL ORGANS AND PLACENTA
    人女性生殖器官和胎盘组织的凝集素标记研究
短句来源

 

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  lectin binding
α-d-Mannopyranoside, α-d-glucopyranoside, and α-d-galactopyranoside were utilized in model studies and product formations were detected by lectin binding.
      
Newly established human pancreatic carcinoma cell lines and their lectin binding properties
      
Quantification and preservation of lectin binding by isolated cardiomyocytes
      
Our aim was to develop a protocol which retained lectin binding to an extent similar to living cells.
      
We tested glutaraldehyde and paraformaldehyde in different concentrations before and after lectin binding, different buffers and divalent cations, as additives, to determine the effects on preservation of lectin binding.
      
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Receptors of wheat germ agglu-tinin (WGA) and soybean agglutinin (SBA) are present on the denuded living egg surface of Rana amurensis.Before cleavage these receptors dis-tribute evenly all over the animal pole (Plate I.Fig.1).At the onset of cleavage,numerous bright fluores-cent granules which correspond to the protrusions in the scanning ele-ctron microscope (10),are seen on the cleavage stripe and many bright ra-diated rays which is identified as folds,are visible on the side of the cleavage stripe (Plate...

Receptors of wheat germ agglu-tinin (WGA) and soybean agglutinin (SBA) are present on the denuded living egg surface of Rana amurensis.Before cleavage these receptors dis-tribute evenly all over the animal pole (Plate I.Fig.1).At the onset of cleavage,numerous bright fluores-cent granules which correspond to the protrusions in the scanning ele-ctron microscope (10),are seen on the cleavage stripe and many bright ra-diated rays which is identified as folds,are visible on the side of the cleavage stripe (Plate I.Fig.2).Soon after,the new membrane hav-ing much less FITC-lectins binding ability,appears and divides the clea-vage stripe into 3 bright lines.The middle one is the primitive contrac-tile ring.While the cleavage goes on,the new membrane increases gra-dually (Plate I.Fig.3).However,when the denuded eggs are immersed in high concentration of WGA (500γ/ml) before cleavage,the new mem-brane appears only for a short time,then vanishes,leaving a black streak (Plate I.Fig.6 b,d).These results are in line with those found in newt's eggs (5).In high concentration of SBA (100 γ/ml) the new membrane exposed continuously but in a much smaller schedule.The contractile ring initially working normally,fails to work,and the new membrane bulges out (Plate II.Fig.7 & 8).Finally the new membrane disappears as those in WGA (Plate.I.Fig.6 a & c are untreated control). In a few FITG-SBA stained eggs,a large dark halo,its center often coincides with the center of the furrow,appears quickly (Plate II.Fig.12),and persists for several minutes to more than one hour,so that it may overlap with the halos seen on the second cleavage stripes.The darker overlapping area gradually decreases and disappear eventually while the ??halos on the second cleavage furrows move away.Sometimes a bright halo may be seen on the egg surface.As the halo contracts,it becomes brighter and brighter.Finally many radiated stripes and a nipple-like structure are found in the center of the halo (Plate II.Fig.10-11).A few receptors,however,remains or lags behind dur-ing the contraction of the bright halo.This indicates that the receptor can become mobile or increase its moving rate under certain condition.The increasing of fluorescence,however,is not always resulting from the mo-vement of the receptor but from the movement of cell surface.By push-ing the evenly distributed fluorescent egg surface,many brighter lines appear at the folded places.This is explain-ed in figure 1.Increasing in fluores-cent intensity due to the fold of egg surface is also present naturely suchas the bright radiated rays and gra-nules at the cleavage stripe. Fig.1.Digrammatical representative of the dif-ferent intensity of fluorescence on the plain and fold region."A" Plain region."B" Fold or protuberant region."." FITC-lectin binding site."I" Transverse section,binding sites inequal distance."II" Top view.Binding sites becoming dense or fluorescence becoming brighter in B region.Indicating the increase of the intensing of fluo-rescence resulting from the move-ment of the binding sites itself.

林蛙卵表面有WGA和SBA受体,胞质分裂前受体分布均匀,开始分裂时预定分裂沟处出现明亮的光带,此时或稍后,在FITC-SBA染的卵中,少数细胞表面会出现一个以分裂沟中心为圆心的暗晕。暗晕的直径刚出现时就有近半个卵球那么大,以后并不明显增大,暗晕的出现和消失相当快,存在的时间长短不一(有的长达1—2小时),第二次分裂沟上也会出现暗晕,当第二次分裂沟的一端向外延伸时,整个暗晕也随之外移。在某种情形下,细胞膜上的受体可移向某一中心,出现亮晕。高浓度的WGA和SBA能使分裂沟中的新膜很快消失,分裂沟成为线状痕迹。高浓度的SBA还能使收缩环逐渐失去作用,新膜随之突出。

The influence of some saccharides, glycosides, glycoproteins and lectins on the binding of ~(125)I-labelled Trichosanthes lectin with acidtreated Sepharose 6B or human placental cell membrane were compared. At low concentrations, among saccharides so far tested, lactose was the strongest inhibitor; melibiose and raffinoae were as strong as the monosaccharide galactose; cellobiose, maltose and sucrose had no effect. Three glycoproteins have been tested, their inhibitory capacities in decreasing order...

The influence of some saccharides, glycosides, glycoproteins and lectins on the binding of ~(125)I-labelled Trichosanthes lectin with acidtreated Sepharose 6B or human placental cell membrane were compared. At low concentrations, among saccharides so far tested, lactose was the strongest inhibitor; melibiose and raffinoae were as strong as the monosaccharide galactose; cellobiose, maltose and sucrose had no effect. Three glycoproteins have been tested, their inhibitory capacities in decreasing order were: porcine thyroglobulin, human serum transferrin and hen ovalbumin. Unlabelled Trichosanthes leotin and ricin I, another galactose-binding leotin, competed with labelled Trichosanthes lectin; however Con A and Pinellia lectin which bound to glucose/mannose and mannan, respectively, did not. It seems reasonable to conclude that Trichosanthes lectin bound mainly to galactose, and its binding site could fit galactose and another sugar residue, but such a disaccharide had to possess a specific conformation, such as that of lactose.

本文报道一些糖类、糖苷、糖蛋白和几种外源凝集素对标记天花粉凝集素和酸处理交联琼脂糖或胎盘细胞膜结合的影响。在低浓度时,所用的糖类中,乳糖是最强的抑制剂,蜜二糖和棉籽糖的抑制能力和乳糖相仿,而纤维二糖、蔗糖、麦芽糖,则无明显影响。三个所用的糖蛋白,它们的抑制活性以下列顺序递减:猪甲状腺球蛋白,人血清转铁蛋白,鸡卵白蛋白。未标记的天花粉凝集素和蓖麻凝集素,两者都专一地和半乳糖结合,它们都能竞争标记天花粉凝集素,而伴刀豆球蛋白A和半夏凝集素则不能竞争。由此,我们推测天花粉凝集素主要是和半乳糖结合,但与乳糖的结合能力最强,故推测其结合部位能容纳半乳糖和另一个单糖。

Squash (Cucurbita moschata Duch.) stigma was of the dry-type. When it wastreated with detergent, proteinase and lectin, pollen could not germinate, or pollentube growth was arrested.The binding of Con A-FITC to the stigma surface indicatedthe presence of lectin-binding sites which midght play an important role in the pollen-pistil interactions. The squash stigma segments stimulated pollen germination invitro, which suggested the presence of certain stimulants in squash stigma tissue....

Squash (Cucurbita moschata Duch.) stigma was of the dry-type. When it wastreated with detergent, proteinase and lectin, pollen could not germinate, or pollentube growth was arrested.The binding of Con A-FITC to the stigma surface indicatedthe presence of lectin-binding sites which midght play an important role in the pollen-pistil interactions. The squash stigma segments stimulated pollen germination invitro, which suggested the presence of certain stimulants in squash stigma tissue. When squash stigma was pollinated with pollen from plants of different speciesother than squash, the pollen (tube) growth was arrested at different sites. Thehemagglutination activity was detected in the mature pistil, while no activity wasfound in the immature one. The extract of pistil inhibited the germination of tobac-co (Nicotiana tabacum) pollen. The inhibition could be relieved by complementarysugars oflectin. It was suggested that pistil lectin might be involved in the inhibitionof tobacco pollen tube by squash pistil. To investigate the action of lectin, exogenous lectin was applied. It was foundthat Con A inhibited the germination of squash pollen and the inhibitory effect wasremoved by α-methyl-D-mannoside. When pollen was labeled with Con A-FITC,the label was bound to the pollen tube. Con A-FITC was also bound to the isolatedpollen wall fragment, but no fluorescence was observed after it was treated withalkali. It was concluded that lectin might inhibit pollen germination by bindingto the glycoprotein or polysaccharide components of the pollen wall.

南瓜柱头表面经去垢剂、蛋白酶及Con A处理后花粉不能萌发或花粉管生长受阻,Con A能专一地与柱头表面结合。柱头块加入培养液可促进花粉萌发。不同的远缘花粉授粉后在雌蕊不同部位受阻。在成熟南瓜雌蕊提取液中检测到血凝活性,凝集素可能参与雌蕊对远缘花粉的抑制。

 
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