Four lectins (WGA, SBA, PNA and Con A) conjugated with horseradish peroxidase were employed to study the distribution of lectin binding in the testis and epididymis of man, SD rat, CFW mouse, rabbit, dog and Rhesus monkey.
Biotinylated lectins:UEA Ⅰ, RCA Ⅰ, DBA, PSA, PNA, BSL, LCA, WGA, ConA AND SBA were used as probes to identify lectin binding sites and to determine if lectin binding patterns change with age in the developing of human fetal esophageal epithelium (8W, 14W, 20W, 28W and 32W). Lectin binding was visualized in fomalin fixed and avidin peroxidase staining procedures.
采用十种生物素化凝集素 U EA- I、 DBA、 PSA、 BSL、 PNA、 L CA、 RCA- I、SBA、 Con A及 WGA分别对 8W、1 4 W、 2 0 W 2 8W及 32 W的人胎儿食管上皮进行研究 ,以确定胎儿食管上皮在发育过程中凝集素的结合位点以及结合方式随年龄的变化。
The distribution of lectin binding in gastrointestinal tract of human, rat and mouse was investigated using horseradish peroxidase labeled lectins-WGA, SBA, and PNA staining method.
Four lectins (WGA, SBA, PNA and Con A) conjugated with horseradish peroxidase were employed to study the distribution of lectin binding in the testis and epididymis of man, SD rat, CFW mouse, rabbit, dog and Rhesus monkey.
α-d-Mannopyranoside, α-d-glucopyranoside, and α-d-galactopyranoside were utilized in model studies and product formations were detected by lectin binding.
Newly established human pancreatic carcinoma cell lines and their lectin binding properties
Quantification and preservation of lectin binding by isolated cardiomyocytes
Our aim was to develop a protocol which retained lectin binding to an extent similar to living cells.
We tested glutaraldehyde and paraformaldehyde in different concentrations before and after lectin binding, different buffers and divalent cations, as additives, to determine the effects on preservation of lectin binding.