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lectin binding
相关语句
  凝集素
    Research on artocarpus lingnanensis lectin binding proteins in human urine and its clinical significance.
    尿液中红桂木凝集素结合蛋白的检测及临床意义
短句来源
    Objeclive and Methods Lectin affinity precipitation method was established and used for the determination of the lectin binding ratios of serum alkaline phosphatase.
    目的 建立一种简便的糖蛋白糖链分析方法———凝集素亲和沉降法。 方法 用凝集素亲和沉降法分别检测血清碱性磷酸酶的L PHA、LCA和DSA凝集素结合率。
短句来源
    The lectin binding patterns of 7 genera of fungi with 12 kinds of lectin in 35 nail lysates revealed different characteristics between genera.
    3 2例溶甲涂片真菌 7个属与 12种凝集素的结合形式具有属间区别。
短句来源
  凝集素结合
    Research on artocarpus lingnanensis lectin binding proteins in human urine and its clinical significance.
    尿液中红桂木凝集素结合蛋白的检测及临床意义
短句来源
    Objeclive and Methods Lectin affinity precipitation method was established and used for the determination of the lectin binding ratios of serum alkaline phosphatase.
    目的 建立一种简便的糖蛋白糖链分析方法———凝集素亲和沉降法。 方法 用凝集素亲和沉降法分别检测血清碱性磷酸酶的L PHA、LCA和DSA凝集素结合率。
短句来源
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  lectin binding
α-d-Mannopyranoside, α-d-glucopyranoside, and α-d-galactopyranoside were utilized in model studies and product formations were detected by lectin binding.
      
Newly established human pancreatic carcinoma cell lines and their lectin binding properties
      
Quantification and preservation of lectin binding by isolated cardiomyocytes
      
Our aim was to develop a protocol which retained lectin binding to an extent similar to living cells.
      
We tested glutaraldehyde and paraformaldehyde in different concentrations before and after lectin binding, different buffers and divalent cations, as additives, to determine the effects on preservation of lectin binding.
      
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The structure of sugar chains of transferrin isolated from sera of pregnant women were analyzed by the dot-lectin-binding assay with Datura stramonium agglutinin and Lens culinaris lectin coupled to horseradish peroxidase. Highbranched and core-fucosylated structures were found in the transferrin of these pregnant women. Moreover, the content of the transferrin with high-branched and core-fucosylated glycan progressively increased in the course of pregnancy.These results can be very important...

The structure of sugar chains of transferrin isolated from sera of pregnant women were analyzed by the dot-lectin-binding assay with Datura stramonium agglutinin and Lens culinaris lectin coupled to horseradish peroxidase. Highbranched and core-fucosylated structures were found in the transferrin of these pregnant women. Moreover, the content of the transferrin with high-branched and core-fucosylated glycan progressively increased in the course of pregnancy.These results can be very important for further studies on the physiological implications of high-branched and core-fucosylated glycans of transferrin.

用欧曼陀罗凝集素(DSA)和小扁豆凝集素(LCA)点印迹方法,分析孕妇血清中传铁蛋白(Tf)的糖链结构的变化。发现孕妇的Tf的糖链含有多天线及核心岩藻糖(Fuc),其含量随着怀孕时间的增加而增多。这些结果为进一步研究含有核心Fuc及多天线结构的糖链的Tf的生理意义奠定了基础。

Objeclive and Methods Lectin affinity precipitation method was established and used for the determination of the lectin binding ratios of serum alkaline phosphatase.Results The results showed that the within run CV of L PHA、LCA and DSA binding ratios of serum alkaline phosphatase were 4 1%,2 3% and 3 4%,between run CV 5 7%,38% and 4 3%,respectively.The correlation cefficents with the results determined by lectinaffinity chromatography were 0 985,0 980 and 0 978,respectively.In...

Objeclive and Methods Lectin affinity precipitation method was established and used for the determination of the lectin binding ratios of serum alkaline phosphatase.Results The results showed that the within run CV of L PHA、LCA and DSA binding ratios of serum alkaline phosphatase were 4 1%,2 3% and 3 4%,between run CV 5 7%,38% and 4 3%,respectively.The correlation cefficents with the results determined by lectinaffinity chromatography were 0 985,0 980 and 0 978,respectively.In addition,L PHA、LCA and DSA binding ratios of serum alkaline phosphatase were determined in 30 healthy individuals and 57 patients with various liver diseases by lectin affinity precipitation meathod.Conclusion The results indicate that lectin affinity precipitation method is simple,rapid and suitable for the use in clinical laboratory.

目的 建立一种简便的糖蛋白糖链分析方法———凝集素亲和沉降法。方法 用凝集素亲和沉降法分别检测血清碱性磷酸酶的L PHA、LCA和DSA凝集素结合率。结果 批内CV值分别为 4 1%、 2 3%和 3 4% ,批间CV值分别为 5 7%、3 8%和 4 3 % ,与凝集素亲和层析法测定结果的相关系数分别为 0 985、 0 980和 0 978。并用此法对 30例健康者和 5 7例不同肝病患者血清碱性磷酸酶L PHA、LCA和DSA凝集素结合率进行了测定。结论 表明凝集素亲和沉降法简便、快速、可成批检测 ,适合临床实验室使用

Objective\ ALL HRP Sandwich assay was developed for the measurement of Artocarpus lingnanensis lectin binding proteins in human urine(U LBP) Methods \ ALL HRP was prepared and purified by Sephadex G 200 chromatography The optimum concentration of coating ALL and the working dilution degree of ALL HRP were determined for the measurement of U LBP With Sandwich enzyme linked assay intra and inter run precision studies were performed The standard curve was prepared for U LBP Results\ The working...

Objective\ ALL HRP Sandwich assay was developed for the measurement of Artocarpus lingnanensis lectin binding proteins in human urine(U LBP) Methods \ ALL HRP was prepared and purified by Sephadex G 200 chromatography The optimum concentration of coating ALL and the working dilution degree of ALL HRP were determined for the measurement of U LBP With Sandwich enzyme linked assay intra and inter run precision studies were performed The standard curve was prepared for U LBP Results\ The working concentrafion range of the standard curve was 9 4~150 μg/ml for U LBP The value of U LBP/Cr(mg/mmol)in normal human urine or patient′s urine with SLE nephropathy,acute or chronic glomerulonephritis(AGN or CGN),nephrotic syndrome,hematuria,pyelonephritis,diabetes mellitus,were determined with ALL HRP Sandwich enzyme linked assay respectively The value of U LBP/Cr(mg/mmol)in normal human urine was 3 44±1 56 mg/mmol,the value of U LBP/Cr(mg/mmol)in SLE nephropathy patient urine,44 06±32 47 mg/mmol was highest;secondly,AGN,38 07±26 13 mg/mmol;nephrotic syndrome 32 57±20 33 mg/mmol;hematuria 25 30±14 62 mg/mmol;pyelonephritis 17 37±11 77 mg/mmol;CGN 18 03±13 70 mg/mmol;diabetes mellitus 15 69±12 08 mg/mmol;respectively The comparison of the value of U LBP/Cr between patients and normal human was distinctly differential Intra assay CV 8 96%,inter assay CV 9 89% Conclusion\ The measurement on U LBP in urine by Sandwich enzyme linked assay with ALL HRP is simple and precise,can be used in wide range The assay on U LBP in urine is important for the diagnosis on some kidney diseases

目的 建立酶标记红桂木凝集素 (ALL HRP)夹心法检测尿液中ALL结合蛋白。方法 制备ALL HRP并经SephadexG 2 0 0纯化作为酶标凝集素 ,ALL为包被物 ,确定最适包被浓度及ALL HRP工作稀释度 ,夹心酶联检测尿液中ALL结合蛋白 (U LBP)。经精密度、重现性等实验并制备标准曲线。结果 以U LBP作标准制备的标准曲线线性检测范围为 9 4~ 15 0 μg/ml。本法测定几种疾病的尿U LBP/Cr( μg/ μmol) ,以SLE肾病为最高 4 4 0 6± 3 2 4 7,其次是急性肾炎 3 8 0 7± 2 6 13 ,肾病综合征 3 2 5 7± 2 0 3 3 ,血尿 2 5 3 0± 14 62 ,慢性肾炎 18 0 3± 13 70 ,糖尿病 15 69±12 0 8,与正常对照组相比 ,均有显著性差异。本法批内CV为 8 86% ,批间CV为 9 89%。结论 ALL HRP夹心酶联法检测U LBP方法简便 ,结果稳定 ,可望在临床上推广使用。

 
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