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lectin binding
相关语句
  凝集素
    Immunohistochemical analysis of lectin binding in vulvar dystrophy and squamous cell carcinoma of the vulva
    外阴营养不良、外阴鳞状上皮细胞癌凝集素标记免疫组化分析
短句来源
  凝集素标记
    Immunohistochemical analysis of lectin binding in vulvar dystrophy and squamous cell carcinoma of the vulva
    外阴营养不良、外阴鳞状上皮细胞癌凝集素标记免疫组化分析
短句来源
  凝集素标记
    Immunohistochemical analysis of lectin binding in vulvar dystrophy and squamous cell carcinoma of the vulva
    外阴营养不良、外阴鳞状上皮细胞癌凝集素标记免疫组化分析
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  lectin binding
α-d-Mannopyranoside, α-d-glucopyranoside, and α-d-galactopyranoside were utilized in model studies and product formations were detected by lectin binding.
      
Newly established human pancreatic carcinoma cell lines and their lectin binding properties
      
Quantification and preservation of lectin binding by isolated cardiomyocytes
      
Our aim was to develop a protocol which retained lectin binding to an extent similar to living cells.
      
We tested glutaraldehyde and paraformaldehyde in different concentrations before and after lectin binding, different buffers and divalent cations, as additives, to determine the effects on preservation of lectin binding.
      
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Eight—five cases of endometriai adenocarcinoma,8 normal and 2 embryonic endometrium were studied with 12 biotin-labelled lectins by using the avidin-biotin-peroxidase complex(ABC) method. The results showed that the lectin-binding sites in normal endometria were localized to glandular luminal border,but in cytoplasm and increased distinctly in case of carcinomas; Con A,RCA WGA receptors were all positive for embryonic, normal,cancerous endometria and were possibly related to endomerial origin; BSL,...

Eight—five cases of endometriai adenocarcinoma,8 normal and 2 embryonic endometrium were studied with 12 biotin-labelled lectins by using the avidin-biotin-peroxidase complex(ABC) method. The results showed that the lectin-binding sites in normal endometria were localized to glandular luminal border,but in cytoplasm and increased distinctly in case of carcinomas; Con A,RCA WGA receptors were all positive for embryonic, normal,cancerous endometria and were possibly related to endomerial origin; BSL, PHA,SBA and PNA receptors were associated with histologic differentiated degree of the carcinomas; LCA, PSA receptors were associated with carcinoma invasion. LCA,PSA, PHA receptors were related to lymph node metastases of the carcinomas, The measurement of lectin receptors may be useful in the clinical practice.

应用12种生物素化的凝集素对85例子宫内膜腺癌、8例正常宫内膜和2例胚胎宫内膜进行免疫组织化学研究。结果发现:①正常宫内膜的凝集素受体分布于腺体的腔缘,腺癌宫内膜的凝集素受体主要分布于细胞浆内,且比正常宫内膜有明显增加;②Con A、RCA、WGA在胚胎、正常及腺癌宫内膜中均呈阳性反应,可能与子宫内膜的组织发生源相关;③BSL、SBA、PHA、PNA受体与宫内膜腺癌的分化程度相关。④LCA、PSA受体丢失与宫内膜腺癌的肌层浸润有关;LCA、PSA、PHA受体缺损与宫内膜腺癌的淋巴结转移有关。这些结果对宫内膜腺癌的糖连接物研究和治疗可能有一定的指导意义。

Purpose To explore the characteristics of the lectins binding in hyperplastic vulvar dystrophy and lichen sclerosis epidemis and vulvar squamous cell carcinoma. Methods Hyperplastic vulvar dystropy,lichen sclerosus and squamous carcinoma were labelled by eight lectins with the technique of immunohistochemistry.Results There was a weak labelling in cell membrane of the epidemis with hyperplastic vulvar dystrophy with concanavalin agglutinin (ConA),lens cuinars agglutinin(LCA), peanut agglutinin(PNA), rieinus...

Purpose To explore the characteristics of the lectins binding in hyperplastic vulvar dystrophy and lichen sclerosis epidemis and vulvar squamous cell carcinoma. Methods Hyperplastic vulvar dystropy,lichen sclerosus and squamous carcinoma were labelled by eight lectins with the technique of immunohistochemistry.Results There was a weak labelling in cell membrane of the epidemis with hyperplastic vulvar dystrophy with concanavalin agglutinin (ConA),lens cuinars agglutinin(LCA), peanut agglutinin(PNA), rieinus communis agglutinin(RCA 1) and soybean agglutinin(SBA), an intermediate intensity of labelling was found in the nuclear membrane with ConA, PNA and wheat germ agglutinin(WGA). Cellular membrane of lichen sclerostic epidemis of vulvar dystrophy was weakly stained with ConA, RCA 1, SBA and WGA, but not LCA. Eight in 12 specimens were binded weakly with uiex europaeus agglutinin(UEA 1). All the 8 lectins were able to bind with vulvar squamous cell carcinoma(VSCC), in which 8 in 12 cases of VSCC were combined weakly with dolichos biflorus agglutinin(DBA). Intensive labelling appeared in the membrane of cancer cells with ConA, UEA 1, LCA and RCA 1, but epithelial pearls were negative for UEA 1.Conclusions Immunohistochemical staining of lectins can be used as a new marker in the diagnosis and differential diagnosis of vulvar dystrophy of hyperplastic and lichen sclerostic types, and squamous cell carcinoma. There is a similar lectin labelling pattern between hyperplastic vulvar dystrophy and vulvar squamous cell carcinoma, which suggests that abnormal lectin binding may be an early event in the vulvar carcinogenesis.

目的 :探讨外阴营养不良增生型、硬化苔藓型表皮细胞和外阴鳞状上皮细胞癌等细胞膜结构与凝集素受体结合表达特征及它们三者之间的关系。方法 :对慢性外阴营养不良表皮增生型、苔藓硬化型及外阴鳞状上皮细胞癌 3种状态下的细胞进行了 8种凝集素免疫组织化学标记、分析、比较。结果 :外阴营养不良增生型表皮各层细胞细胞膜与刀豆凝集素 (ConA)、扁豆凝集素 (LCA)、花生凝集素 (PNA)、蓖麻凝集素 (RCA 1)和大豆凝集素 (SBA)结合强度弱 ,其表皮细胞细胞核膜与ConA、PNA和麦胚凝集素 (WGA)有中等强度结合。外阴营养不良硬化苔藓型表皮各层细胞细胞膜与ConA、RCA 1、SBA和WGA结合强度弱 ,而与LCA不结合 ,其 12例中的 8例与荆豆凝集素 (UEA 1)发生较弱的结合。外阴鳞状上皮细胞癌癌细胞与 8种凝集素均结合 ,其 12例中的 7例其癌细胞膜与双花扁豆凝集素 (DBA)弱结合 ,癌细胞膜与UEA 1强结合 ,但癌巢中央细胞团不与UEA 1结合 ,癌细胞细胞核膜与ConA、LCA、RCA 1呈强结合。结论 :凝集素标记免疫组织化学染色可作为诊断、鉴别诊断外阴营养不良增生型、硬化苔藓型和外...

目的 :探讨外阴营养不良增生型、硬化苔藓型表皮细胞和外阴鳞状上皮细胞癌等细胞膜结构与凝集素受体结合表达特征及它们三者之间的关系。方法 :对慢性外阴营养不良表皮增生型、苔藓硬化型及外阴鳞状上皮细胞癌 3种状态下的细胞进行了 8种凝集素免疫组织化学标记、分析、比较。结果 :外阴营养不良增生型表皮各层细胞细胞膜与刀豆凝集素 (ConA)、扁豆凝集素 (LCA)、花生凝集素 (PNA)、蓖麻凝集素 (RCA 1)和大豆凝集素 (SBA)结合强度弱 ,其表皮细胞细胞核膜与ConA、PNA和麦胚凝集素 (WGA)有中等强度结合。外阴营养不良硬化苔藓型表皮各层细胞细胞膜与ConA、RCA 1、SBA和WGA结合强度弱 ,而与LCA不结合 ,其 12例中的 8例与荆豆凝集素 (UEA 1)发生较弱的结合。外阴鳞状上皮细胞癌癌细胞与 8种凝集素均结合 ,其 12例中的 7例其癌细胞膜与双花扁豆凝集素 (DBA)弱结合 ,癌细胞膜与UEA 1强结合 ,但癌巢中央细胞团不与UEA 1结合 ,癌细胞细胞核膜与ConA、LCA、RCA 1呈强结合。结论 :凝集素标记免疫组织化学染色可作为诊断、鉴别诊断外阴营养不良增生型、硬化苔藓型和外阴鳞状上皮细胞癌新的指标。具有恶变潜能的增生型外阴营养不良与外阴鳞状上皮细胞癌 ,两者细胞核膜凝集素标记有相似之处 ,提示在外阴组织的癌变过?

 
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