Gastric tissue spomens of 190 cases were examined by mucinohistochemistry tochemis of AB (pH 1.0) KOH/PAS and immunohistochemical ABC technique of lectin binding.
Histochemical detection of lectin binding was carried out using ABC method, in condyloma accuminata,The lectins used for demonstrating sugar resi- dues were: ConA, PNA, 12CA 1, SBA and UEA 1. Leetin binding in normal squmaous epithelium showed reginoal distribution patterns of keratinized, spinous and basal layer types.
Histochemical localization of lectin binding was generally at cellular surface and in the intercellular substances and sometimes in the cytoplasm of normal epithelial cells.
Histochemical localization of lectin binding was generally at cellular surface and in the intercellular substances and sometimes in the cytoplasm of normal epithelial cells.
The cytochemical distribution of epithelia~1 lectin binding may be indicative of the expression of nomal stratification and keratinocytic differentiation, and the disappearance of this typical epithelial pattern may suggest severe dysplasia.
Gastric tissue spomens of 190 cases were examined by mucinohistochemistry tochemis of AB (pH 1.0) KOH/PAS and immunohistochemical ABC technique of lectin binding.
The new classification of intestinal metaplasia based on lectin binding demonstrated that the metaplasia was predominantly metaplasia of colonic (ConA + PNA-) and mixed types (ConA +PNA + ) in Carcinoma free gastric mucosa of either intestinal or diffuse carcionma. Some of the diffuse gastric carcinomas may originate from the intestinal meiaplasia.
84.0% lymph node metastatic tumors produced celluar glycoconjugates, whichdiffered in lectin binding profiles from those made by the cells in the respective primary carcinoma. More PNAand PHA receptors were produced by the lymph node metastatic tumors than primary carcinoma. ConclusionThe positive rares PNA and PHA receptors are relative to the degree of invasion of bladder carcinoma and lymphnode metastatic tumors.
Gastric tissue spomens of 190 cases were examined by mucinohistochemistry tochemis of AB (pH 1.0) KOH/PAS and immunohistochemical ABC technique of lectin binding.
The new classification of intestinal metaplasia based on lectin binding demonstrated that the metaplasia was predominantly metaplasia of colonic (ConA + PNA-) and mixed types (ConA +PNA + ) in Carcinoma free gastric mucosa of either intestinal or diffuse carcionma. Some of the diffuse gastric carcinomas may originate from the intestinal meiaplasia.
84.0% lymph node metastatic tumors produced celluar glycoconjugates, whichdiffered in lectin binding profiles from those made by the cells in the respective primary carcinoma. More PNAand PHA receptors were produced by the lymph node metastatic tumors than primary carcinoma. ConclusionThe positive rares PNA and PHA receptors are relative to the degree of invasion of bladder carcinoma and lymphnode metastatic tumors.
α-d-Mannopyranoside, α-d-glucopyranoside, and α-d-galactopyranoside were utilized in model studies and product formations were detected by lectin binding.
Newly established human pancreatic carcinoma cell lines and their lectin binding properties
Quantification and preservation of lectin binding by isolated cardiomyocytes
Our aim was to develop a protocol which retained lectin binding to an extent similar to living cells.
We tested glutaraldehyde and paraformaldehyde in different concentrations before and after lectin binding, different buffers and divalent cations, as additives, to determine the effects on preservation of lectin binding.
After studying sophora japonica agglutinin receptor and carcinoembryonic antigen in nasal inverted papillomas, and compared with normal nasal mucosae, nasal polyps and nasal papillary adenocarcinomas, it was found that lectin-binding pattern of inverted papilloma was intermediate between normal mucosa and adenocarcinoma, and carcinoembryonic antigen was positive as well as adenocarcinoma was, wkile normal nasal mucosa and nasal polyp negative. So it suggests that nasal inverted papilloma is a kind...
After studying sophora japonica agglutinin receptor and carcinoembryonic antigen in nasal inverted papillomas, and compared with normal nasal mucosae, nasal polyps and nasal papillary adenocarcinomas, it was found that lectin-binding pattern of inverted papilloma was intermediate between normal mucosa and adenocarcinoma, and carcinoembryonic antigen was positive as well as adenocarcinoma was, wkile normal nasal mucosa and nasal polyp negative. So it suggests that nasal inverted papilloma is a kind of potentially malignant neoplasm and especial attention must be taken in the clinical treatment of this disease.
Histochemical detection of lectin binding was carried out using ABC method, in condyloma accuminata,The lectins used for demonstrating sugar resi- dues were: ConA, PNA, 12CA 1, SBA and UEA 1. Leetin binding in normal squmaous epithelium showed reginoal distribution patterns of keratinized, spinous and basal layer types. Histochemical localization of lectin binding was generally at cellular surface and in the intercellular substances and sometimes in the cytoplasm of normal epithelial cells....
Histochemical detection of lectin binding was carried out using ABC method, in condyloma accuminata,The lectins used for demonstrating sugar resi- dues were: ConA, PNA, 12CA 1, SBA and UEA 1. Leetin binding in normal squmaous epithelium showed reginoal distribution patterns of keratinized, spinous and basal layer types. Histochemical localization of lectin binding was generally at cellular surface and in the intercellular substances and sometimes in the cytoplasm of normal epithelial cells. In condyloma specimans, spinous cells showed an intense ConA staining and moderate binding by other lectins, which was somewhat decreased when compared with that of the normal epithelium. The cytochemical distribution of epithelia~1 lectin binding may be indicative of the expression of nomal stratification and keratinocytic differentiation, and the disappearance of this typical epithelial pattern may suggest severe dysplasia.
An immunoenzymologic method has been applied to the identifi-cation of lectin-binding sites as well as carcinoembryonic antigen(CEA) on paraffin sections of benign and malignant colorectaltissues. There is an inerease of PNA receptor in malignant cell thanin normal epithelial cell, 84% (21/25) of cancer specimens expressedPNA-binding sites. Conversely, the lectin PHA avidly binds to themucin found in well-differentiated goblet cells in the normal colorec-tum, it binds to all...
An immunoenzymologic method has been applied to the identifi-cation of lectin-binding sites as well as carcinoembryonic antigen(CEA) on paraffin sections of benign and malignant colorectaltissues. There is an inerease of PNA receptor in malignant cell thanin normal epithelial cell, 84% (21/25) of cancer specimens expressedPNA-binding sites. Conversely, the lectin PHA avidly binds to themucin found in well-differentiated goblet cells in the normal colorec-tum, it binds to all but 1 colorectal controls (21/22). Being ableto indicate glycosylated disorder of epithelial cell glycoconjugatesin colonic mucosa, the above lectin might help diagnosis of coloniccancer. The experimental result also showed that CEA might bedeveloped into an effective probe into the surveillance of the meta-stasis and recurrence of colonic cancer.