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tetracycline labeling
相关语句
  四环素标记
     The materials were drawn after one month, and all rats were subjected to tetracycline labeling at the 3rd, the 13rd days before drawing.
     1个月后取材,并在取材前第3天、第13天行四环素标记
短句来源
  四环素荧光标记
     Bone scintigraphy was carried out at different postoperative time,combined with tetracycline labeling,microangiography with Indian ink and HE staining.
     术后不同时间进行ECT检查,结合四环素荧光标记、印度墨汁灌注、HE染色等处理。
短句来源
     Strong tetracycline labeling around the interface of BMP-HA group was observed, which indicated active formation of new bone.
     BMP-HA组的界面有较强的四环素荧光标记,显示界面成骨活跃。
短句来源
  “tetracycline labeling”译为未确定词的双语例句
     METHODOLOGY 15 chronic hemodialysis patients were involved in this study in whom bone biopsy were performed after double tetracycline labeling and serum biochemical parameter were measured at the same time.
     方法 :对 15例血液透析患者行骨活检 ,进行骨活检标本骨组织形态计量学检测 ,同时测定某些血生化指标。 结果 :15例血液透析患者均有程度不等、变化不一的骨组织学病理变化 ;
短句来源
     Results Fibroblasts seeded on the PGA foams formed 3-dimensional matrix 3 weeks after seeding,which was demonstrated as ossous tissue by tetracycline labeling and ARS staining.
     结果诱导3周后三维网架上的成纤维细胞表达成骨表型,分泌成骨细胞特征性标志物:骨钙素(OCN)和骨特异性碱性磷酸酶(B-AKP);
短句来源
     Compared to the control side, the tetracycline labeling lines were wider; the increase of the expression level of BMP-2 appeared earlier, greater and lasted longer in the tension area of the experimental side.
     自体荧光染色结果显示实验侧移动牙张力侧新形成骨量多于对照侧,骨形成蛋白(BMP)免疫组化染色显示,实验侧BMP-2表达增强现象较对照侧出现早,峰值高,而且持续的时间长。
短句来源
     The histological analysis and SEM photomicrograph showed active bone formation 4 weeks and 8 weeks after implantation, as well as tetracycline labeling under fluoroscopy analysis in SW group.
     细胞载体复合体植入动物体内后,SW组载体表面有类骨组织形成,而对照组HA载体表面无骨组织形成;
短句来源
  相似匹配句对
     On L(p,q)-labeling of graphs
     图的L(p,q)-标号问题
短句来源
     TUNEL labeling, H. E.
     为探讨猪发情周期不同时期卵泡发育和闭锁的规律及机理 ,本实验通过TUNEL原位标记、H .
短句来源
     APPLICATION OF THE MICROWAVE DISCHARGE METHOD FOR THE SPECIFIC ~3H-LABELING——I. PREPARATION OF [7-~3H] TETRACYCLINE
     微波定位氚标记——Ⅰ.[7-~3H]四环素的制备
短句来源
     The histochemical study on the nodules with the specific labeling of tetracycline fluorescence indicated that the nodules were composed of new bones.
     趋骨性四环素特异性标记为新生骨组织;
短句来源
     Polarographic Determination of Tetracycline
     四环素的极谱法测定
短句来源
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  tetracycline labeling
A bone biopsy specimen indicated osteomalacia; no tetracycline labeling could be seen along most trabecular bone surfaces, and the ratio of total osteoid volume to bone volume was increased (71.8%).
      
After a follow-up of 1, 3, or 6 weeks, the femurs were examined radiographically, histologically, histomorphometrically, and microradiographically, and also used in tetracycline labeling studies.
      
Histomorphometric analysis after double-tetracycline labeling was completed in six patients (one male and five females) after the exclusion of those who dropped out because of adverse events unrelated to the test drug, or refusal of continuation.
      
The aim of this study was to evaluate bone resorption (BR) in rats by two methods: chronic 3H-tetracycline labeling (3HTC) and pyridinium crosslink excretion (PYDX), and compare the sensitivity of these markers in two age groups.
      
We present iliac bone histomorphometric data after in vivo double tetracycline labeling in 21 men aged 34-74 with significant spinal osteoporosis as evidenced by compression spinal fracture without significant trauma.
      
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Objective To establish an animal model of microwave hyperthermic devitalization of long bones and compare bone scintigraphy of the devitalised bone with pathologic examination. Method 16 Mongrel adult dogs were used. Enght 8 cm of one femur of each dog was exposed and isolated by heat proof material.Two microwave antennas were inserted into the medullary cavity at a distance of 2 5 cm,and the bone was heated intermittently to maintain a surface temperature of 50℃ to 60℃ for 30 minutes.Bone scintigraphy...

Objective To establish an animal model of microwave hyperthermic devitalization of long bones and compare bone scintigraphy of the devitalised bone with pathologic examination. Method 16 Mongrel adult dogs were used. Enght 8 cm of one femur of each dog was exposed and isolated by heat proof material.Two microwave antennas were inserted into the medullary cavity at a distance of 2 5 cm,and the bone was heated intermittently to maintain a surface temperature of 50℃ to 60℃ for 30 minutes.Bone scintigraphy was carried out at different postoperative time,combined with tetracycline labeling,microangiography with Indian ink and HE staining. Result About 5 cm~6 cm of segmental femur was deprived of blood supply after devitalization which was confirmed by microangiography and negative labeling of tetracyclines.At two weeks, the devitalised bone demonstrated a cold region on the scintiscan and no revascularization in microangiography.12~16 weeks after the operation,partial devitalised bone was revascularized and HE staining showed widel bone resorption and minimum formation of new bone.However,the radionuclide uptakes of the devitalised segments were greater than those of the normal femurs.At oneyear,the devitalised segment was well(not completely) revascularised and showed evident appositional new bone formation.The scintigraphic bone imaging was slightly lower than that of the normal femur. Conclusion A strong positive scintiscan in early stage might demonstrate a good potential of revascularization of the heat devitalised cortical bone rather than its viability.And the scintiscans of long term follow up more than one year should be more informative.

目的建立长骨微波高温灭活的动物模型,将其再血管化过程的核素骨扫描(ECT)表现与病理评价进行对比。方法选用成年杂种犬16条,游离一侧股骨约8cm,隔热材料保护周围组织,间距25cm置入微波天线2根,间断加热,维持骨皮质表面温度50~60℃30分钟。术后不同时间进行ECT检查,结合四环素荧光标记、印度墨汁灌注、HE染色等处理。结果通过微血管造影、四环素荧光标记等方法证实约有5cm~6cm骨段在灭活后完全失去血运。灭活骨段2周时无明显再血管化,ECT表现为冷区。12~16周灭活骨段部分恢复血运,HE染色显示以骨吸收为主,仅见少量成骨,但ECT显像均超过健侧对照。术后1年灭活骨段已恢复丰富血运伴明显原位性新骨形成,ECT显像强度略低于健侧对照。结论早期ECT的强阳性表现并不能反应热灭活皮质骨的活性,但却反应了良好的再血管化能力,1年以上的长期随访结果更有价值

OBJECTIVE To evaluate the morbidity and classification of renal osteodystrophies in hemodialysis patients according to bone histomorphometric changes and find more sensitive and specific boichemical parameters to help assess bone metabolic states.METHODOLOGY 15 chronic hemodialysis patients were involved in this study in whom bone biopsy were performed after double tetracycline labeling and serum biochemical parameter were measured at the ...

OBJECTIVE To evaluate the morbidity and classification of renal osteodystrophies in hemodialysis patients according to bone histomorphometric changes and find more sensitive and specific boichemical parameters to help assess bone metabolic states.METHODOLOGY 15 chronic hemodialysis patients were involved in this study in whom bone biopsy were performed after double tetracycline labeling and serum biochemical parameter were measured at the same time. RESULTS We found that every patient has abnormal bone histomorphometric changes,iPTH has positive correlation with MAR、BFR( r =0 585, P <0 05; r =0 436, P <0 05).Bone alkaline phosphatase(BAP) had a better relation to OS/BS%、O.Ar/B.Ar% than ALP( r =0 701 vs r =0 523, P <0 001; r =0 827 vs r =0 809, P <0 001).There was a statistical difference between low turn over and not low turn over bone disease in serum iPTH.Serum aluminum of the two ABD patients was in the normal range,the increment of serum aluminum after DFO test in aluminum bone disease patients was higher and the serum iPTH lower than non ABD patients. CONCLUSION iPTH、BAP could be used to evaluate bone metabolic changes;in conjunction with iPTH assay,DFO test could be applied to help diagnosis ABD.But there was no absolute distinction on biochemical data among various renal bone diseases.Therefore bone biopsy is still a gold standard for classification of renal osteodystriphies.

目的 :了解肾性骨营养不良在血液透析患者中的发病及分布情况 ,观察血生化指标与骨组织形态学有关参数的相关性。  方法 :对 15例血液透析患者行骨活检 ,进行骨活检标本骨组织形态计量学检测 ,同时测定某些血生化指标。  结果 :15例血液透析患者均有程度不等、变化不一的骨组织学病理变化 ;全段甲状旁腺激素(intactparathyroidhormone ,iPTH)与骨矿化率、骨形成率具有相关性 (r=0 5 85 ,P <0 0 5 ;r =0 436 ,P <0 0 5 )。骨特异性碱性磷酸酶 (bonealkalinephosphatase ,BAP)、碱性磷酸酶 (alkalinephosphatase ,ALP)均与类骨质表面、类骨质面积呈正相关关系 ,前者相关性更好 (r=0 70 1vs 0 5 32 ;r=0 82 7vs 0 80 9)。血iPTH在低转化骨病与非低转化骨病两组间差异显著 (P <0 0 1)。 2例铝中毒骨病患者 ,血清铝在正常范围内 ,去铁胺试验后血铝增加值较非铝中毒者高 ,而且iPTH水平较低。  结论 :iPTH、BAP结合起来可以初步估计骨代谢状...

目的 :了解肾性骨营养不良在血液透析患者中的发病及分布情况 ,观察血生化指标与骨组织形态学有关参数的相关性。  方法 :对 15例血液透析患者行骨活检 ,进行骨活检标本骨组织形态计量学检测 ,同时测定某些血生化指标。  结果 :15例血液透析患者均有程度不等、变化不一的骨组织学病理变化 ;全段甲状旁腺激素(intactparathyroidhormone ,iPTH)与骨矿化率、骨形成率具有相关性 (r=0 5 85 ,P <0 0 5 ;r =0 436 ,P <0 0 5 )。骨特异性碱性磷酸酶 (bonealkalinephosphatase ,BAP)、碱性磷酸酶 (alkalinephosphatase ,ALP)均与类骨质表面、类骨质面积呈正相关关系 ,前者相关性更好 (r=0 70 1vs 0 5 32 ;r=0 82 7vs 0 80 9)。血iPTH在低转化骨病与非低转化骨病两组间差异显著 (P <0 0 1)。 2例铝中毒骨病患者 ,血清铝在正常范围内 ,去铁胺试验后血铝增加值较非铝中毒者高 ,而且iPTH水平较低。  结论 :iPTH、BAP结合起来可以初步估计骨代谢状态 ;而在铝中毒骨病的诊断上 ,去铁胺试验结合血iPTH水平有一定帮助。但以上血生化指标在不同类型的骨病中并无严格的界限 ,所以 ,骨活检仍是诊断肾性骨营养不良的金标准。

OBJECTIVE Renal osterodystrophy is a common complication of chronic renal failure in which different pathogenesis is involved.The histologic classification is important in the choice of specific treatments.The purpose of the study was to analyze the clinical manifestations and the influencing factors of bone diseases in maintenance hemodialysis(HD)patients. METHODOLOGY Forty nine quantitative HD patients dialyzed for 92 3±43 3(30~206)months were involved...

OBJECTIVE Renal osterodystrophy is a common complication of chronic renal failure in which different pathogenesis is involved.The histologic classification is important in the choice of specific treatments.The purpose of the study was to analyze the clinical manifestations and the influencing factors of bone diseases in maintenance hemodialysis(HD)patients. METHODOLOGY Forty nine quantitative HD patients dialyzed for 92 3±43 3(30~206)months were involved in this study.The average age was 53 8±11 4(33~78)years,male to female ratio 38∶11.Bone biopsy was perfomed after double tetracycline labeling in all the patients,and biochemical parameters as serum levels of calcium,AKP and intact parathyroid hormone (iPTH) were determined. RESULTS According to the bone histology and biochemistry,24 cases(49 0%)were classified as high turnover bone disease(HTBD),19 cases(38 8%)as low turnover bone disease(LTBD),and the other 6 cases(12 2%)as mixed uremic osteodystrophy(MUO).Aluminum deposition(bone surface aluminum staining≥30%)was detected in the bone tissue of 31 cases(63 3%)of whole patients,with 84 2% in LTBD,54 2% in HTBD and 33 3% in MUO.HTBD was more frequent in patients dialyzed over 5 years,with a prevalence of 83 3% in those dialyzed over 10 years.The bone diseases in all the 4 diabetic patients were classified as LTBD,and patients with a long history of active vitamin D 3 administration were also frequently classified as LTBD.Patients on high flux hemodialysis had higher level of serum calcium( P <0 05).No correlation was found between the dialysis duration and the presence of aluminum deposition.Serum phosphorus and iPTH levels were markedly decreased in the aluminum deposition positive patients as compared to those in the negative patients. CONCLUSION HTBD was the most common type of renal bone diseases in this group of patients,and aluminum deposition positive rate was high as 63 3%.HTBD was associated with longer dialysis duration,deficiency of active vitamin D 3,while LTBD with improper use of active vitamin D 3 and diabetes.Aluminum deposition was found associated not with the duration of hemodialysis treatment,but with the aluminum administration history.

目的 :研究维持性血液透析患者肾性骨病的类型、临床特点并分析相关因素。  方法 :49例血液透析患者 ,口服四环素标记后行骨活检 ,同时检测相关生化参数。  结果 :49例患者均有肾性骨病的临床表现 ,其中高转化型 2 4例 (49 0 % ) ,低转化型 19例 (38 8% ) ,混合型 6例 (12 2 % )。三组患者年龄和性别构成无差异 ,4例糖尿病患者均为低转化型 ;透析时间超过 10年者高转化型骨病发生率显著高于透析 5~ 10年和≤ 5年者。骨铝染色阳性率为 6 3 3% ,低转化型高于高转化型及混合型组 ,高通量组和普通透析组骨病类型和骨铝染色无差异 ,高通量组透析前血钙水平显著高于普通组 (P <0 0 5 ) ,iPTH水平低于普通组 ,但无统计学差异。低转化型骨病组服用活性维生素D3 制剂的比例显著高于其它组。骨铝阳性组透析前血磷和iPTH水平显著低于阴性组 ,服用铝制剂的患者比例也高于骨铝阴性组。  结论 :本组患者高转化型骨病与长时间透析、维生素D3 不足等因素相关 ,低转化型骨病可能与维生素D3 制剂使用不当、糖尿病等因素相关 ,而骨铝沉积则与服用铝制剂等因素相关 ,与透析时...

目的 :研究维持性血液透析患者肾性骨病的类型、临床特点并分析相关因素。  方法 :49例血液透析患者 ,口服四环素标记后行骨活检 ,同时检测相关生化参数。  结果 :49例患者均有肾性骨病的临床表现 ,其中高转化型 2 4例 (49 0 % ) ,低转化型 19例 (38 8% ) ,混合型 6例 (12 2 % )。三组患者年龄和性别构成无差异 ,4例糖尿病患者均为低转化型 ;透析时间超过 10年者高转化型骨病发生率显著高于透析 5~ 10年和≤ 5年者。骨铝染色阳性率为 6 3 3% ,低转化型高于高转化型及混合型组 ,高通量组和普通透析组骨病类型和骨铝染色无差异 ,高通量组透析前血钙水平显著高于普通组 (P <0 0 5 ) ,iPTH水平低于普通组 ,但无统计学差异。低转化型骨病组服用活性维生素D3 制剂的比例显著高于其它组。骨铝阳性组透析前血磷和iPTH水平显著低于阴性组 ,服用铝制剂的患者比例也高于骨铝阴性组。  结论 :本组患者高转化型骨病与长时间透析、维生素D3 不足等因素相关 ,低转化型骨病可能与维生素D3 制剂使用不当、糖尿病等因素相关 ,而骨铝沉积则与服用铝制剂等因素相关 ,与透析时间长短无关。

 
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