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   guide black fur sheep 的翻译结果: 查询用时:0.192秒
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guide black fur sheep
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  贵德黑裘皮羊
     The Establishment of Fibroblast Cell Line and Its Biological Characteristic Research in Guide Black fur sheep
     贵德黑裘皮羊耳成纤维细胞系的建立和生物学特性的研究
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     guide
     商情指南
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     Black,M.
     Black,M.
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     GUIDE TO PROPERTY
     置业指南
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     The Beautiful Black
     美丽的黑色
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     The Establishment of Fibroblast Cell Line and Its Biological Characteristic Research in Guide Black fur sheep
     贵德黑裘皮羊耳成纤维细胞系的建立和生物学特性的研究
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Taking Guide Black fur sheep ear margin as the study materials.we succeeded in establishing a fibroblast cell line by the method of explant adherence culture and cell cryoconservation technology.Observations on morphology,dynamic growth,determination of viability,analysis of karyotype,test of microorganism were carried out.The results showed :Population Doubling Time(PDT)of cells was approximately 36 h,Cell viability before freezing was 96.2%,The fibroblasts were frozen in DMEM/F-12+20%FBS by adding 10%...

Taking Guide Black fur sheep ear margin as the study materials.we succeeded in establishing a fibroblast cell line by the method of explant adherence culture and cell cryoconservation technology.Observations on morphology,dynamic growth,determination of viability,analysis of karyotype,test of microorganism were carried out.The results showed :Population Doubling Time(PDT)of cells was approximately 36 h,Cell viability before freezing was 96.2%,The fibroblasts were frozen in DMEM/F-12+20%FBS by adding 10% DMSO,After thawing Cell viability was 93.6%,The attachment rates of thawed cells after 24h was 85%.When cultured up to passage ten,Diploid(2 n=54)account for 82%~90% in cells chromosome.tests for microbial contamination of bacteria,fungi and yeasts,virus and mycoplas ma were negative.The test result of mycoplasma was negative.The newly established cell line not only preserved the gemlplasm resources of this important Guide Black fur sheep on the cell level,but also provided valuable materials for the research of somatic cloning.

以贵德黑裘皮羊耳缘组织为材料,采用组织块贴壁培养法和细胞冷冻技术成功构建了成纤维细胞系,并对培养细胞进行了形态学、生长动力学观察、细胞活力测定、核型分析和微生物检测。结果表明:细胞群体倍增时间(PDT)约为36h,冻存前细胞活力为96.2%,以DMEM/F-12+20%FBS中添加10%DMSO冻存成纤维细胞,解冻后细胞活力为93.6%,24h后的贴壁率为85%。在传代10次之前,细胞染色体中二倍体(2n=54)占主体,约为82%~90%,细菌、真菌、病毒、支原体检测为阴性。该细胞系的建立,使贵德黑裘皮羊这一国家重要种质资源在细胞水平上保存下来,也为体细胞克隆等研究提供了理想的生物材料。

 
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