osteogenesis phenotype 成骨表型(2)   成骨表型      AIM: To observe the fibroblast NIH3T3 transfected by the same signal molecule core -binding factor α1 (Cbfa1) gene of the osteogenesis induced by cytokine and the possibility of changes of its osteogenesis phenotype.      目的:观察细胞因子诱导成骨的共同信号分子核心结合因子α1基因转染NIH3T3成纤维细胞,其成骨表型转变的可能性。 短句来源      Conclusions The elevation of Ca2+i in fibroblast may play a important role in regulating the cooperation and restriction among the cbfa1 and other growth factors ,promoting the proliferation, differentiation, and the expression of osteogenesis phenotype.      认为这种[Ca2+]i浓度的变化在影响成纤维细胞中cbfa1和某些生长因子之间的相互关系、促进细胞增殖、分化及成骨表型转换中可能起重要作用。 短句来源   “osteogenesis phenotype”译为未确定词的双语例句      It indicates that the osteogenesis phenotype of NIH3T3 have the repair effect on bone defect in vitro.      (3) 明胶海绵移植组; 短句来源   相似匹配句对      3.Cortical osteogenesis;      ③皮质骨成骨 ; 短句来源      Intraoral distraction osteogenesis      口内入路的颌骨牵引成骨技术 短句来源      Roles of Cell Cycle Regulatory Factors in Differentiating Osteoblasts Phenotype During Mandibular Distraction Osteogenesis      细胞周期调节因子在下颌骨牵张成骨细胞表型分化中的作用      It indicates that the osteogenesis phenotype of NIH3T3 have the repair effect on bone defect in vitro.      (3) 明胶海绵移植组; 短句来源      Objective To identify the p phenotype.      目的　鉴定 p血型。 短句来源 查询“osteogenesis phenotype”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 没有找到相关例句 AIM: To observe the fibroblast NIH3T3 transfected by the same signal molecule core -binding factor α1 (Cbfa1) gene of the osteogenesis induced by cytokine and the possibility of changes of its osteogenesis phenotype. METHODS:The experiment was done in the Central Laboratory of Department of Orthopaedics of Xinqiao Hospital of Third Military Medical University of Chinese PLA from October 2003 to October 2004. The gene trasfection of Cbfa1 to fibroblast NIH3T3 was performed with liposomes trasfection technique.... AIM: To observe the fibroblast NIH3T3 transfected by the same signal molecule core -binding factor α1 (Cbfa1) gene of the osteogenesis induced by cytokine and the possibility of changes of its osteogenesis phenotype. METHODS:The experiment was done in the Central Laboratory of Department of Orthopaedics of Xinqiao Hospital of Third Military Medical University of Chinese PLA from October 2003 to October 2004. The gene trasfection of Cbfa1 to fibroblast NIH3T3 was performed with liposomes trasfection technique. The expression of osteogenesis representative gene: I Collagen, Osteocalcin, bone sialoprotein (BSP), alkaline phosphatase (AKP), and Cbfa1 mRNA were detected with reverse transcription-polymerase chain reaction (RT-PCR). The expression of osteocalcin was detected with immunocytochemical stain. The expression of Cbfa protein was detected with Westernblot. RESULTS: ① The expression of osteocalcin, bone sialoprotein and alkaline phosphatase appeared after the trasfection of Cbfa1 gene to fibroblast NIH3T3. The reinforcement of I type collagen expression indicated that the expression of osteogenesis representative gene existed in the transcription level. ② The expression of osteocalcin was detected by immunocytochemical stain, which showed that the NIH3T3 cells had developed the osteogenesis cells. ③ The expression of Cbfa1 protein was detected by Westernblot, which confirmed that the phenotype changes had occurred on fibroblast. CONCLUSION: Gene transfer of Cbfa1 to fibroblast NIH3T3 can alter the conversion of the phenotype of osteogenesis, and then the quantity of osteoblast increased and made the increase of osteogenesis ability possible. 目的:观察细胞因子诱导成骨的共同信号分子核心结合因子α1基因转染NIH3T3成纤维细胞,其成骨表型转变的可能性。方法:实验于2003-10/2004-10在解放军第三军医大学新桥医院骨科中心实验室完成。采用脂质体转染技术行核心结合因子α1基因转染NIH3T3成纤维细胞,成骨标志基因I型胶原、骨钙素、骨唾液蛋白、碱性磷酸酶、核心结合因子α1mRNA的表达采用反转录-聚合酶链反应检测,骨钙素的表达采用免疫细胞化学染色检测,Cbfa蛋白的表达采用Westernblot检测。结果:①NIH3T3成纤维细胞经核心结合因子α1基因转染后出现骨钙素、骨唾液蛋白、碱性磷酸酶的表达,I型胶原表达增强,说明在转录水平有成骨标志基因的表达。②免疫细胞化学染色检测到了骨钙素的表达,显示NIH3T3细胞已发展为成骨细胞。③Westernblot检测到了核心结合因子α1蛋白的表达,证实了成纤维细胞发生了表型的改变。结论:核心结合因子α1基因转移NIH3T3成纤维细胞改变了成纤维细胞向成骨表型转化,增加了成骨细胞数量,使成骨能力增强成为可能。 Objective To study the effects of fluoride on the level of Ca2+ release in cultured fibroblast. Methods Fibroblasts were cultured and loaded with fluo-3-AM. Ca2+i was measured by fluorescent intensity in each cell with laser confocal microscope. Results In the group of 1 mg/LF-, the intercellular free calcium concentration in fibroblasts increased transitorily. Conclusions The elevation of Ca2+i in fibroblast may play a important role in regulating the cooperation and restriction among the cbfa1 and other growth... Objective To study the effects of fluoride on the level of Ca2+ release in cultured fibroblast. Methods Fibroblasts were cultured and loaded with fluo-3-AM. Ca2+i was measured by fluorescent intensity in each cell with laser confocal microscope. Results In the group of 1 mg/LF-, the intercellular free calcium concentration in fibroblasts increased transitorily. Conclusions The elevation of Ca2+i in fibroblast may play a important role in regulating the cooperation and restriction among the cbfa1 and other growth factors ,promoting the proliferation, differentiation, and the expression of osteogenesis phenotype. 目的研究氟对成纤维细胞Ca2+浓度的影响并探讨其意义。方法应用扫描共聚焦显微技术测定不同染氟条件下(0.0001、1、10mg/LF-)成纤维细胞Ca2+水平的变化。结果染氟1mg/L组成纤维细胞在染氟500s左右时[Ca2+]i水平开始升高,约1500s达到高峰,然后开始下降,3000s左右回到起点位置。结论成纤维细胞染氟1mg/LF-组[Ca2+]i有一过性升高。认为这种[Ca2+]i浓度的变化在影响成纤维细胞中cbfa1和某些生长因子之间的相互关系、促进细胞增殖、分化及成骨表型转换中可能起重要作用。    相关查询 osteogenesis phenotype inductive osteogenesis immunogenetic phenotype laboratory phenotype lymphocyte phenotype phenotype switching mutant phenotype character phenotype phenotype reversion metabolic phenotype acetylation phenotype

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