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ribosomal dna restriction analysis
相关语句
  核糖体dna限制性分析
     They are mainly denaturing gradient gel electrophoresis(DGGE),temperature gradient gel electrophoresis(TGGE),single-strand conformation polymorphism(SSCP),randomly amplified polymorphic DNA(RAPD),terminal restriction fragment length polymorphism(T-RELP),amplified ribosomal DNA restriction analysis(ARDRA) etc.
     这些技术主要有变性梯度凝胶电泳(DGGE)/温度梯度凝胶电泳(TGGE)、单链构象多态性(SSCP)、随机引物扩增多态性DNA(RAPD)、限制性片段长度多态性(RFLP)和扩增核糖体DNA限制性分析(ARDRA)等。
短句来源
     With a combined approach of phospholipid fatty acid(PLFA),community level physiological profiles(CLPPs) and amplified ribosomal DNA restriction analysis(ARDRA),this paper evaluated the effects of methamidophos continuously applied for 2 and 4 years on the structural,functional and genetic diversities of soil microbial communities.
     利用磷脂脂肪酸(PLFA)、群落水平生理活性(CLPPs)和扩增核糖体DNA限制性分析(ARDRA)标记,综合评估低浓度和高浓度甲胺磷连续施用2和4 yr后对土壤微生物群落结构、功能和遗传多样性的影响。
短句来源
  “ribosomal dna restriction analysis”译为未确定词的双语例句
     Diversity and its spatial distribution of bacteria in 0~5 cm,5~10 cm and 10~15 cm depths of non-tillage paddy fields were studied based on the molecular method of ARDRA(amplified ribosomal DNA restriction analysis).
     采用基因指纹图谱ARDRA分析和RFLP分析,对免耕水稻土壤中的细菌多样性及其在0~5、5~10、10~15cm土层的空间分布进行了研究。
短句来源
     Biotyping and Genotyping of GV were done according to Briselden's method and ARDRA(Amplified ribosomal DNA restriction analysis)respectively.
     根据Briselden方法对GV进行生物学分型; 运用ARDRA方法(扩增rDNA限制性分析)对GV进行基因分型。
短句来源
     All the isolates were capable of growing on a synthetic medium with phenol (500 ~ 800 mg L -1 ) as a sole carbon source and classified into 4 operational taxoriomic units (OTUs) by amplified ribosomal DNA restriction analysis (ARDRA).
     它们均能在以苯酚(500~800 mg L-1)为唯一碳源的无机盐培养基上生长,其16S rDNA基因的ARDRA多态性分析将这些分离物划分成4个分类操作单元(OTUs).
短句来源
     These isolates were analyzed by amplified ribosomal DNA restriction analysis(ARDRA) and classified into 16 types. The partial nucleotide sequences of 16S rDNA were determined and analyzed in GenBank database.
     通过限制性内切酶酶切分析(amplified ribosomal DNA restriction analysis,ARDRA),共将其分为16种类型,对这16种类型的代表菌株16S rDNA的部分序列(500bp)进行基因测序,并将测序结果在GenBank中比对,分析其在系统发育分类学上的地位。
短句来源
     The methods of colony counting in dilution culture, soil enzyme activity measurement and amplified ribosomal DNA restriction analysis (ARDRA) were synthetically used to investigate the microbial activities and the bacterial community in paddy soil.
     为了研究水稻土和非水稻土中微生物区系的差别,使用稀释平板计数法分析了太湖周围四处水稻土及其对照土中的细菌、兼性厌氧细菌、放线菌和真菌的数量。
短句来源
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  相似匹配句对
     Analysis of RFLP in Ribosomal DNA of Vitis
     葡萄属植物核糖体基因的RFLP分析
短句来源
     Identification and sequencing of ribosomal DNA-ITS of Phytophthora sojae in Fujian
     福建省大豆疫病病原鉴定及其核糖体DNA-ITS序列分析
短句来源
     Application of Its and 16S Ribosomal DNA to Environmental Microbial Ecology
     综合应用ITS及16S rDNA进行环境微生物生态研究
短句来源
     That is rough DNA.
     95℃水浴加热10min,得到DNA粗提物。
短句来源
     Sequence analysis of ribosomal DNA ITS of clinical common Fusarium species.
     临床常见镰刀菌rDNA ITS序列分析
短句来源
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  ribosomal dna restriction analysis
Microbial communities were analysed by community level physiological profiling (CLPP), using the Biolog system, and by the amplified ribosomal DNA restriction analysis (ARDRA) approach.
      
Changes in microbial community were detected by ribosomal intergenic spacer analysis (RISA) and amplified ribosomal DNA restriction analysis (ARDRA), which revealed that the introduced E.
      
And microbial community dynamics of augmented systems was revealed by amplified ribosomal DNA restriction analysis (ARDRA), a modern DNA fingerprint technique.
      
Statistical comparisons of libraries based on Amplified Ribosomal DNA Restriction Analysis (ARDRA) data revealed no significant differences between the communities recovered in the non-biodegraded (NBD) and highly biodegraded oils (HBD).
      
A combined approach, which included amplified ribosomal DNA restriction analysis (ARDRA), ribotyping and polymerase chain reaction (PCR) with species-specific oligonucleotide primers was applied.
      
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100 isolates were obtained from feed water solid media using spread-plate method with diluted activated sludge ( 10 -5 dilution rate). All the isolates were capable of growing on a synthetic medium with phenol (500 ~ 800 mg L -1 ) as a sole carbon source and classified into 4 operational taxoriomic units (OTUs) by amplified ribosomal DNA restriction analysis (ARDRA). The full-length 16S rDNA gene of one representative strain for each OTU was amplified, cloned, sequenced and blasted against the GenBank...

100 isolates were obtained from feed water solid media using spread-plate method with diluted activated sludge ( 10 -5 dilution rate). All the isolates were capable of growing on a synthetic medium with phenol (500 ~ 800 mg L -1 ) as a sole carbon source and classified into 4 operational taxoriomic units (OTUs) by amplified ribosomal DNA restriction analysis (ARDRA). The full-length 16S rDNA gene of one representative strain for each OTU was amplified, cloned, sequenced and blasted against the GenBank database. The results showed that 97 isolates were closely related to Alcaligenes faecalis with 99% homology; two isolates showed 99% identity to the sequence of Arthrobacter nicotianae and Klebsiella sp. respectively; and the last one exhibited 96% homology with Ochrobactrum sp. The specific amplification of the gene encoding the large subunit of the multi-component phenol hydroxylase (LmPHs) revealed that three out of four OTUs employed the metabolic pathway catalyzed by multicomponent phenol hydroxylase to degrade phenol, while the specific amplification from the Arthrobacter nicotianae-like strain did not show any products, indicating a possibly different phenol-degrading pathway. Fig 2, Tab 2, Ref 11

用未经处理的含酚焦化工业废水为基础配制培养基,采用平板涂布法,在10~(-5)稀释度下得到100个单菌落分离物.它们均能在以苯酚(500~800 mg L-1)为唯一碳源的无机盐培养基上生长,其16S rDNA基因的ARDRA多态性分析将这些分离物划分成4个分类操作单元(OTUs).各单元代表株的16S rRNA基因序列分析和检索结果表明,97个分离物与Alcaligenes faecalis同源性达99%,2个分离物分别与Arthrobacter nicotianae和Klebsiella sp.99%同源,另一个分离物与Ochrobactrum sp.96%同源.苯酚羟化酶大亚基基因(LmPHs)PCR扩增表明,除类似Arthrobacter nicotianae的菌株外,其它3个分类操作单元的降酚菌都能利用多组分苯酚羟化酶代谢途径进行酚代谢.图2表2参11

Both PCR-TGGE(temperature gradient gel electrophoresis,TGGE) and 16S rRNA gene clone library construction were used to comparatively analyze the microbial communities of a water injection well(WW)and an oil well(OW)in Dagang oilfield. TGGE analysis of the PCR amplified 16S rDNA V3 region products showed great difference between these two microbial communities. Six major bands were detected in the TGGE profile of the WW sample, while only one predominant band in the OW sample was found. Two 16S rRNA gene...

Both PCR-TGGE(temperature gradient gel electrophoresis,TGGE) and 16S rRNA gene clone library construction were used to comparatively analyze the microbial communities of a water injection well(WW)and an oil well(OW)in Dagang oilfield. TGGE analysis of the PCR amplified 16S rDNA V3 region products showed great difference between these two microbial communities. Six major bands were detected in the TGGE profile of the WW sample, while only one predominant band in the OW sample was found. Two 16S rRNA gene clone libraries were also constructed, and 108 and 50 clones were selected from the WW and OW library respectively for amplified ribosomal DNA restriction analysis (ARDRA). 33 taxanomic operational units (OTUs) were found in the WW library with 6 major OTUs, while only 8 OTUs were found in the OW library with one OTU predominant. The results of TGGE and clone library profiling analysis both indicated that microbial community of the WW had higher diversity than the OW. Sequence analysis of the representative clone of each OTU showed that most bacteria of the WW were affiliated with α, β, and γProteobacteria andActinobacteria, especiallyRhodobacter(47%). Most bacteria of the OW were affiliated with α, β, and γProteobacteria, especiallyPseudomonas(62%).Molecular analysis of the microbial diversity in oilfield provides foundation for better application of MEOR (Microbial Enhanced Oil Recovery).

通过多聚酶链式反应 温度梯度凝胶电泳(PCR TGGE)和构建16SrRNA基因克隆文库两种方法对比研究了大港油田孔二北断块注水井和采油井的微生物群落结构。16SrDNAV3区PCR扩增产物的TGGE图谱分析表明,这两个油井的微生物群落结构差异很大。注水井样品的TGGE图谱中有6条主要条带,而采油井样品中只有一个条带占绝对优势。同时,建立了两个样品的16SrRNA基因克隆文库,从中分别挑选了10 8和5 0个克隆进行限制性酶切片段长度多样性分析(ARDRA)。注水井样品有33个操作分类单元(OTU) ,其中6个OTU是优势类型;而采油井样品只有8个OTU ,有1个OTU在文库中占绝对优势。克隆文库和TGGE的研究结果一致,均表明注水井样品的微生物多样性比采油井丰富很多。每个OTU的代表克隆序列分析结果表明,注水井样品中的细菌主要属于α、β、γ变形菌纲和放线菌纲,尤其是红细菌亚纲( 4 7% )。采油井样品的细菌主要属于α、β、γ变形菌纲,尤其是假单胞菌属( 6 2 % )。油藏微生物多样性的分子分析可为开展微生物采油技术研究奠定基础

Objective To investigate whether hemolytic bacteria existed in individuals without clinical symptoms.Methods A dynamic investigation and molecular characterization was performed on the occurrence of hemolytic bacteria in 17 individuals without clinical symptoms over a three weeks period.The mapping patterns were generated with ARDRA(Amplified Ribosomal DNA Restriction Analysis) analysis of 16S rDNA of those hemolytic bacteria.ERIC-PCR fingerprinting was also performed to furt her differentiate t...

Objective To investigate whether hemolytic bacteria existed in individuals without clinical symptoms.Methods A dynamic investigation and molecular characterization was performed on the occurrence of hemolytic bacteria in 17 individuals without clinical symptoms over a three weeks period.The mapping patterns were generated with ARDRA(Amplified Ribosomal DNA Restriction Analysis) analysis of 16S rDNA of those hemolytic bacteria.ERIC-PCR fingerprinting was also performed to furt her differentiate t hese hemolytic bacteria on a strain level.Results Hemolytic bacteria was not isolated in twelve individuals,but was detected in four individuals(A~D) only once,and in one individual(E) all the five times in t he study.Two types of mapping patterns were generated with ARDRA analysis of 16S rDNA of those hemolytic bacteria isolated from five individuals(A~E).Type I showed 99% homology with Bacillus cereus ATCC 10987,which was detected only in individual A.Type II showed 99% homology with E.coli CFT073,which was detected in individual B,C,and D.Both of the two types were detected in individual E.Conclusion This study showed that hemolytic bacteria were also present in healthy human fecal samples,and its presence may be relevant to the wellness the individual.

目的调查无临床症状健康人肠道内有无溶血菌的存在,并利用分子方法对溶血菌进行鉴定。方法通过血平板分离培养,对17例无临床症状个体肠道内溶血菌的存在情况做了3周的动态调查,对分离到的溶血菌的16S rDNA进行ARDRA(Am p lified R ibosom a l DNA R estriction A na lys is)酶切分型,将溶血菌分为不同类型,最后利用ER IC-PCR指纹图谱技术对不同类型的溶血菌进行菌株水平的鉴定。结果17例个体中发现5例个体能检测到溶血菌,其中4例个体(A~D)只有1次样品检测到溶血菌,而个体E在3周的5次采样中均能检测到溶血菌。根据ARDRA酶切图谱将溶血菌分为2种类型,Ⅰ型16S rDNA全长测序后与蜡状芽胞杆菌(B acillus cereus ATCC10987)序列同源性达99%,Ⅱ型与大肠埃希菌菌(E scherich ia coli CFT 073)的16S rDNA序列同源性达99%。结论在健康个体肠道内也有溶血菌的存在,且溶血菌的存在可能与机体的疲劳程度和饮食情况有关。

 
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