Thermokinetics of the saponification of diethyl adipate was studied with a conduction calorimeter and the validation of the thermokinetic model in this paper has been verified by the experimental results.

Using the means of microcalorimetry, from the view of thermokinetics, we established thermokinetic model of mutual effect between antitumour drugs and DNA, calculated mutual thermodynamic function.

According to the thermokinetic model,the relationship between the concentration of antibiotics (c) and growth rate constant (k) and its half inhibitory concentration,Ic50,were analyzed quantitatively. Clarithromycin: k=0.03106- 1.273× 10- 3c Ic50=8.81 μ g· mL- 1 (0.5～ 20 μ g· mL- 1) Clarithromycin+ Pr3+ : k=0.02967- 1.332× 10- 3c Ic50=7.38 μ g· mL- 1 (1～ 15 μ g· mL- 1) Clarithromycin+ La3+ : k=0.02741- 1.194× 10- 3c Ic50=6.34 μ g· mL- 1 (1～ 15 μ g· mL- 1) The results of microcalorimetry experiments not only indicated that antibacterial activity of Clarithromycin was stronger than that of erythromycin,but also the changeable features of thermokinetics of the bacterial cell in biological,biochemical and metabolic process under the action of different drugs.

The thermogentic curves were further analyzed by thermokinetic theory to obtain the dynamic parameters and the thermokinetic model on seed germination of Robinia pseudoacacia. The relationship of the thermogenetic power(μw) and the germination time(h) of the germination process of 20 grains Robinia pseudocacia seeds at 25℃ was P=208.77/[0.1937+0.8063exp(-0.06563t)]

Methods Using microcalorimetry, the inhibitory action of different area Coptis Roots on Escherichia coli metabolism was measured at 37℃. In accordance with thermokinetic model, the parameter, such as K, P and I was obtained, and the relation between quantity and effect of Coptis Root in different areas was analyzed.

Methods Using microcalorimetry,the inhibitory action of different area,Rhubarb Roots on Escherichia Coli metabolism was measured at 37℃. In accordance with thermokinetic model,the parameter,such as K and TG was obtained,and the relation between quantity and effect of Rhubarb Roots in different areas was analyzed.

The free-surface temperature history of the melt spinning of copper measured by Tenwick and Davies [3] is compared with those calculated using a thermokinetic model assuming different parameters.

The relationship between the free-surface temperature history and the thermal and kinetic factors involved in melt spinning has been examined using a thermokinetic model for rapid solidification.

A thermokinetic model has been developed, which simplifies the current approach assuming the0-th order and zero activation energy for this process.

The thermogentic curves were further analyzed by thermokinetic theory to obtain the dynamic parameters and the thermokinetic model on seed germination ofRobinia pseudoacacia.

Reduced extent equations for chemical kinetics and thermokinetics of consecutive first order reactions have been deduced and mathematical model for thermokinetics of consecutive first order reactions has been suggested, which can be used to calculate both rate constants and molar enthalpies of two steps simultaneously with thermogram data from asingle experiment. Thermokinetics of the saponification of diethyl adipate was studied with a conduction calorimeter and the validation of the thermokinetic model...

Reduced extent equations for chemical kinetics and thermokinetics of consecutive first order reactions have been deduced and mathematical model for thermokinetics of consecutive first order reactions has been suggested, which can be used to calculate both rate constants and molar enthalpies of two steps simultaneously with thermogram data from asingle experiment. Thermokinetics of the saponification of diethyl adipate was studied with a conduction calorimeter and the validation of the thermokinetic model in this paper has been verified by the experimental results.

Thermokinetic model of two-parameter initial rate method for faster enzyme-cat-alyzed reactions has been suggested based on the two-parameter theoretical model of conduc-tion calorimeter. Thermokinetics of xanthine oxidation catalyzed by xanthine oxidase wasstudied using microcalorimetry. This faster enzyme-catalyzed reaction obeyed the Michaelis-Menten kinetics, and at 298. 15 K and pH 7. 5, the Michaelis constant(Km) for xanthine was1.04×10-3 mol·L-1, which is in agreement with that given in the...

Thermokinetic model of two-parameter initial rate method for faster enzyme-cat-alyzed reactions has been suggested based on the two-parameter theoretical model of conduc-tion calorimeter. Thermokinetics of xanthine oxidation catalyzed by xanthine oxidase wasstudied using microcalorimetry. This faster enzyme-catalyzed reaction obeyed the Michaelis-Menten kinetics, and at 298. 15 K and pH 7. 5, the Michaelis constant(Km) for xanthine was1.04×10-3 mol·L-1, which is in agreement with that given in the literature.

Titration calorimetry is emerging as an important tool for characterizing interactions of biological macromolecules by virtue of its general applicability, high accuracy and precision. In this paper, two mathematical models for thermokinetics of a single-substrate enzyme-catalyzed reaction in titration period and in the stopped-titration reaction period, respectively, have been developed, by using titration calorimetry. On the basis of the titration calorimetric curve, one can use these two models to calculate...

Titration calorimetry is emerging as an important tool for characterizing interactions of biological macromolecules by virtue of its general applicability, high accuracy and precision. In this paper, two mathematical models for thermokinetics of a single-substrate enzyme-catalyzed reaction in titration period and in the stopped-titration reaction period, respectively, have been developed, by using titration calorimetry. On the basis of the titration calorimetric curve, one can use these two models to calculate not only the thermodynamic data ( △rHm) but also the kinetic data (Km and k2) for the reaction. Thermokinetics of a well-studied single-substrate enzymatic reaction, the catalase-catalyzed decomposition of hydrogen peroxide, was thus investigated by titration calorimetry, and the molar enthalpy ( △rHm) was found to be ( - 86.75 × 0.88)kJ mol-1. The Michaelis constant (Km) for H2O2 and the turn - over number of the enzyme (k2) were determined by the titration-period thermokinetic model to be (5.41 ± 0.24) × 10-3 mol·L-1 and (3.58 ± 0.33) × 103s-1, respectively, whereas the corresponding kinetic parameters calculated by the stopped-titration-reaction-period thermokinetic model were (5.43 ±0.21) × 10-3 mol·L-1 and (3.60±0.41) × 103 s-1, respectively, at 298.15 K and pH 7.0. Reliability of the above thermokinetic models was verified by the experimental data.