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universal bacteria
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     Methods: Dental plaque sample were collected from 30 children subject,15 in caries free group(CF,dmft=0),15 in caries susceptible group(CS,dmft≥10). Ribosomal RNAtargeted specificity probes labeled with different fluorescence for universal bacteria and Streptococcus mutans were analysed by fluorescence in situ hybridization(FISH) and flow cytometry(FCM).
     方法:收集无龋(caries free,CF)和高龋(caries susceptib le,CS)儿童牙菌斑各15例,将不同荧光素标记的寡核苷酸探针:细菌通用探针和变形链球菌特异性探针进行荧光原位杂交,结合流式细胞仪检测菌斑中变形链球菌所占的比例。
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  “universal bacteria”译为未确定词的双语例句
     2. According to 16SrDNA, 6 TaqMan probes were designed and synthesized for RTF-PCR detection, including 1 universal phytoplasma probe, 4 specific phytoplasma probes, 1 universal bacteria probe and 1 liberobacter prove.
     2·在国际国内首次根据16SrDNA自行设计合成了实时荧光PCR检测的ThQMan高效Z 检测探针6个,其中植原体广谱探针1个,组特异性探针4个,细菌广谱性探针1个,柑Z 桔黄龙病菌特异性探针1个。 其探针序列均己申请专利保护。
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  相似匹配句对
     Universal Primer PCR for Detection of Clinic Bacteria
     通用引物PCR检测临床常见致病菌的实验研究
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     Endophytic Bacteria
     植物内生细菌
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     On the Universal Value
     关于普世价值的几点认识
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     UNIVERSAL SPACE
     大空间
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     Lysogenic bacteria E.
     溶源性细菌E.
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Objective: To develop a fast and reliable method for the rapid identification and quantification of the major cariogenic bacteria Streptococcus mutans in dental plaque,and provide reference data for accurate evaluation of caries susceptibility or caries activity.Methods: Dental plaque sample were collected from 30 children subject,15 in caries free group(CF,dmft=0),15 in caries susceptible group(CS,dmft≥10).Ribosomal RNAtargeted specificity probes labeled with different fluorescence for universal bacteria...

Objective: To develop a fast and reliable method for the rapid identification and quantification of the major cariogenic bacteria Streptococcus mutans in dental plaque,and provide reference data for accurate evaluation of caries susceptibility or caries activity.Methods: Dental plaque sample were collected from 30 children subject,15 in caries free group(CF,dmft=0),15 in caries susceptible group(CS,dmft≥10).Ribosomal RNAtargeted specificity probes labeled with different fluorescence for universal bacteria and Streptococcus mutans were analysed by fluorescence in situ hybridization(FISH) and flow cytometry(FCM).Results: The percentage of S.mutans in plaque ranged from 1.24 to 3.4 in CF groupe(2.25±0.71) and from 5.04 to 9.7 in CS groupe(7.37±1.34).The statistical analysis of the results indicated significant difference(P<0.01).Conclusion: Fluorescence in situ hybridization,combined with flow cytometry detection,can be used to quantitative analyse of cariogenic S.mutans.

目的:建立快速定量检测菌斑中主要致龋菌变形链球菌的方法,为龋齿活跃性评估提供参考数据。方法:收集无龋(caries free,CF)和高龋(caries susceptib le,CS)儿童牙菌斑各15例,将不同荧光素标记的寡核苷酸探针:细菌通用探针和变形链球菌特异性探针进行荧光原位杂交,结合流式细胞仪检测菌斑中变形链球菌所占的比例。结果:在无龋儿童菌斑中,变形链球菌在总菌中所占比例数是1.24%~3.4%(2.25±0.71),而在高龋儿童菌斑中比例为5.04%~9.7%(7.37±1.34),两组比较有着明显的统计学差异(P<0.01)。结论:流式细胞仪结合荧光原位杂交技术,可作为一种细菌定量检测方法应用于致龋性变形链球菌的定量分析中。

 
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