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   virus type 2 的翻译结果: 查询用时:0.13秒
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virus type
相关语句
  2型病毒
     Sequence determination of E and NS1 gene for 2 strains of dengue virus type 2 and its phylogenetic tree analysis
     两株登革2型病毒E、NS1蛋白基因序列测定及其系统发生分析
短句来源
     Expression and identification of domain B in E protein of dengue virus type 2 isolates in China
     我国登革2型病毒分离株E蛋白B抗原区的表达与鉴定
短句来源
     Objective:To display the envelope protein of dengue virus type 2 strain 43 (D2-43) on yeast cell surface and explore a screening platform for DNA shuffling with yeast surface display system.
     目的 :在酵母细胞表面展示登革 2型病毒 4 3株 (D2_4 3)的E基因 ,探索利用酵母表面展示系统建立DNA改组筛选平台的可行性。
短句来源
     Highly expression of B domain of dengue virus type 2 envelope protein in E.coli
     登革2型病毒包膜蛋白B区在大肠杆菌中的高效表达
短句来源
     Objective:To construct infectious full-length cDNA clone of dengue virus type 2 isolated in China(DEN2-43).
     目的:构建登革2型病毒中国分离株(DEN2-43)的感染性全长cDNA克隆。
短句来源
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  病毒2型
     Objective In order to compare various detection methods for human immunodeficiency virus type 2(HIV-2), 16 cases of suspected HIV-2 infections were follow-up investigated.
     目的通过随访调查16例人类免疫缺陷病毒2型(HIV2)可疑感染者,初步评价HIV2的几种检测方法。
短句来源
     A comparing study of various detection methods for human immunodeficiency virus type 2
     不同人类免疫缺陷病毒2型检测方法的比较研究
短句来源
     Quality control of recombinant adeno-associated virus type 2/human blood coagulation factor IX
     重组腺相关病毒2型/人凝血因子IX的质量研究
短句来源
     Aim: To investigate the abilities of recombinant adeno-associated virus type 2 (rAAV2) transfecting neurospheres.
     目的 :探讨重组腺相关病毒 2型 (rAAV2 )对神经干细胞球的转染能力。
短句来源
     The digoxigenin-labelled cDNA probe of dengue virus type 2 (DEN2) was directly prepared by polymerase chain reaction (PCR). The recombinant plasmid PDEⅡ305 DNA inserted with a fragment of cDNA DEN2 was used as template. A pair of specific primers was applied to prime the amplification of 305bp PDEⅡ305 cDNA.
     用聚合酶链反应(PCR)技术,以重组质粒PDEⅡ305 DNA为模板,在底物中补充标记的脱氧尿嘧啶核苷三磷酸(DIG-dUTP),经扩增反应制备了地高辛配基标记的登革病毒2型(DEN_2)cDNA探针。
短句来源
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  “virus type 2”译为未确定词的双语例句
     SEARCH FOR HERPES SIMPLEX VIRUS TYPE 2 (HSV-2) AND HUMAN PAPILLOMAVIRUS (HPV) IN THE NORMAL AND ABNORMAL CERVICAL SAMPLES
     SEARCH FOR HERPES SIMPLEX VIRUS TYPE2(HSV-2)AND HUMAN PAPILLOMAVIRUS(HPV)IN THE NORMAL AND ABNORMAL CERVICAL SAMPLES
短句来源
     ENHANCEMENT OF MORPHOLOGICAL AND ONCOGENIC TRANSFORMATION OF MOUSE CELLS WITH 12-O-TETRADECANOYL PHORBOL- 13-ACETATE FOLLOWING HERPES SINPLEX VIRUS TYPE 2 INFECTION
     ENHANCEMENT OF MORPHOLOGICAL AND ONCOGENIC TRANSFORMATION OF MOUSE CELLS WITH 12-O-TETRA-DECANOYL PHORBOL-13-ACETATE FOLLOWIN
短句来源
     Results There were 35 cases of virus infection, includ- ing 20 cases of respiratory syncytial virus(RSV)infection(9.5%), 6 of influenza virus infection(2.8%), 9 of adenovirus infection(4.3%), 4 of parainfluenza virus type 3 infection(1.9%), 2 of parainfluenza virus type 1 infection (1%), and 2 of parainfluenza virus type 2 infection (1%).
     结果病毒感染35例,其中呼吸道合胞病毒(RSV)感染20例(9.5%),流感病毒感染6例(2.8%),腺病毒9例(4.3%),副流感病毒Ⅲ型4例(1.9%),副流感病毒Ⅰ、Ⅱ型各2例(1%)。
短句来源
     Genital herpes, a common sexually transmitted disease, is primary caused by h herpes simplex virus type 2 (HSV-2).
     生殖器疱疹(genital herpes,GH)主要由单纯疱疹病毒Ⅱ型(Herpes Simplex Virus Ⅱ,HSV-2)感染所致。
短句来源
     Construction of Recombinant Adeno-Associated Virus Type 2 Containing Ag85A Gene of Mycobacterium tuberculosis
     含结核分枝杆菌Ag85A基因的2型重组腺相关病毒的构建和免疫原性
短句来源
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  virus type
Herpes simplex virus type 2 meningitis without genital lesions: An immunoblot study
      
In spite of the absence of genital lesions, serological studies revealed a primary infection by herpes simplex virus type 2.
      
Herpes simplex virus type 2 infection of neonatal mice
      
We have demonstrated that newborn mice can be infected orally with herpes simplex virus type 2.
      
Transplacental and transvaginal transmission of herpes simplex virus type 2 did not occur in our mice.
      
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A transmissible EHF related agent was discovered in lung tissue of infected Apodemus agrarius in the course of serial passages for EHF virus isolation in Apodemus agrarius from non-endemic area. Antigenic specificity of the isolates was detected by indirect fluorescent antibody technique ( IFAT ):among 101 convalescent sera of EHF patients tested, 96% were positive; 175 sera of normal persons and patients suffering from other diseases were all negative; out of 20 paired sera of EHF patients, 18 showed...

A transmissible EHF related agent was discovered in lung tissue of infected Apodemus agrarius in the course of serial passages for EHF virus isolation in Apodemus agrarius from non-endemic area. Antigenic specificity of the isolates was detected by indirect fluorescent antibody technique ( IFAT ):among 101 convalescent sera of EHF patients tested, 96% were positive; 175 sera of normal persons and patients suffering from other diseases were all negative; out of 20 paired sera of EHF patients, 18 showed 4 fold or more rise of antibody titers; 12 sera of EHF patients tested with KHF antigen previously were reexamined with EHF antigen actually with the same positive or negative results; tests made with antisera against reo-virus (type 1) and reovirus-like agent (orbivirus?) gave consistently negative results; blind tests of coded sera of EHF patients and normal persons produced similar expected results; obvious blocking effect of KHF antiserum to immuno-fluorescence elicited by EHF patient's serum was demonstrated, but no such effect was found with polyvalent antisera of reovirus types 1-3. The above results demonstrated preliminarily that the isolated EHF related agent was the causative agent of EHF.

在用非疫区黑线姬鼠分离EHF病毒的传代中,发现感染鼠鼠肺中存在一种可传代的EHF相关因子。用间接免疫荧光法对其特异性作了检查:101份EHF患者血清96%阳性,175份正常人及其他疾病患者血清全部阴性;20例EHF患者双份血清,18例的恢复期有4倍以上抗体滴度升高;12例EHF患者血清对KHF抗原及EHF相关因子的阴、阳性反应基本一致;抗呼肠孤病毒血清和抗类呼肠孤病毒血清等全部阴性;血清编码双盲法检查和预期结果一致;抗KHF血清对EHF患者血清的免疫荧光反应有显著阻断作用,抗呼肠孤病毒Ⅰ~Ⅲ型多价血清无此作用。上述结果可初步确定,此相关因子是流行性出血热病毒。

A 30,000 dalton glycoprotein (g30K) induced by herpes simplex virus type-2 (HSV-2) was purified on an immunoadsorbent column consisting of the HSV-2 type- specific monoclonal antibody (McAb) CH-A9 linked to seph-arose 4B. Evidence is presented showing that this glycopotein has properties distinct from those assigned to any other HSV glycoprotein. First, its molecular weight is lower than other HSV glycoproteins. And second, it is HSV-2 type-specific antigen.

用抗HSV—Ⅱ型特异性单克隆抗体CH—A9与Sepharose 4B偶联制备免疫吸附柱作亲和层析,纯化出HSV—Ⅱ30,000道尔顿糖蛋白。结果表明,该糖蛋白特性与文献报道的各种HSV糖蛋白均不相同:首先,其分子量明显较小,其次,具有HSV—Ⅱ型特异性。

The purification and characterization of herpes simplex virus type-Ⅰ(HSV-Dindu-ced DNA polymerase (DNA pol) were investigated. Partial purified HSV-Ⅰ DNA polwas obtained from HSV-Ⅰ infected BHK cells by DEAE-cellulose, PⅡ-cellulose ana DNA-cellulose chromatographies. The opti- mum salt concentration and divalent cation magnesium concentration for enzyme activity were 200 mM Kcl and 2mM Mgcl 2 ,and the Km value (michaelis constant) for four substrates was 3μM. The specific inhibitors of HSV-I DNA pol, phosphonoformate...

The purification and characterization of herpes simplex virus type-Ⅰ(HSV-Dindu-ced DNA polymerase (DNA pol) were investigated. Partial purified HSV-Ⅰ DNA polwas obtained from HSV-Ⅰ infected BHK cells by DEAE-cellulose, PⅡ-cellulose ana DNA-cellulose chromatographies. The opti- mum salt concentration and divalent cation magnesium concentration for enzyme activity were 200 mM Kcl and 2mM Mgcl 2 ,and the Km value (michaelis constant) for four substrates was 3μM. The specific inhibitors of HSV-I DNA pol, phosphonoformate and phosphonoacetate, inhibited the enzyme activity by 50% at 0.42μM and 0.46μM respectively, but inhibited L1210 DNApol at 32μM/and 40μM. 1mM N-ethylmaleimide, aspecific inhibitor of DNA/pola, inhibited the activity of L1210 DNA pol by 90%,but exerted no effect on the activity of HSV-1 DNA pol. Twenty seven antibiotics with different inhibition mechanisms were tested in cell free system for their effects on HSV-1 DNA pol. The results indicated that the cell free virus enzyme model possessed certain degree of specificity and this model can be used for screening antiviral antibiotics and other chemicals.

本文报道单纯疱疹病毒Ⅰ型DNA聚合酶(HSV-1 DNA pol)的提纯,特性及药物抑制试验。应用高盐提取,序列通过DEAE-纤维素,P_(11)-纤维素二种离子交换柱层析及DNA-纤维素亲和层析,由HSV-1感染BHK细胞内可制备相当纯的HSV-1 DNA pol。该酶最适盐浓度为200mM KCl;最适镁离子浓度为2mM;对4个底物的米氏常数为3μM。特异的HSV-1 DNA pol抑制剂——膦羧基甲酯及膦羧基乙酯抑制50%酶活性的剂量(ID_(50))分别为0.42μM及0.46μM,而二者抑制L1210 DNA polα的ID_(50)为32μM及40μM。特异的正常细胞DNA α抑制剂——N乙基马来酰亚胺lmM抑制90%L1210 DNA pol α的活性,同样浓度对HSV-1 DNA pol活性无影响。我们选择不同化学类别具有不同作用机制的已知抗生素及其它药物27个,测定了它们在体外抑制HSV-1 DNA pol的作用。结果显示病毒诱导酶的体外模型有相当特异性并对其在筛选抗病毒抗生素中的应用进行了讨论。

 
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