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     The Study of Specific Antibody Labelled Staphylococci-Zs on Rapid Identifying-Group Test of Leptospira
     特异性抗体标记葡萄球菌Z_5株在钩端螺旋体快速定群上的研究
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     The Study of Specific Antibody Labelled Stephylococci No. 1800 Strain on Group-[dentication of N. menigitidis
     特异性抗体标记葡萄球菌№.1800株在脑膜炎球菌分群上的研究
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     RESULTS OF THE APPLICATION OF LABELLED (15)~N AND 32P FERTILIZERS TO YOUNGLINGS OF SLASH PINE AND FOUR CLONES OF HYBRID POPLARS
     黑杨派无性系和湿地松苗施用~(15)N和~(32)P标记肥料的试验
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     PREPARATION OF TRITIUM LABELLED DIHYDROETORPHINE (~3H-DHE) AND TRITIUM LABELLED ETORPHINE (~3H-E)
     氚标记二氢埃托芬(~3H-DHE)及氚标记埃托芬(~3H-E)的制备
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     THE SEGMENTAL DISTRIBUTION OF THE LABELLED SPINAL GANGLIONIC CELLS AFTER HRP INJECTION INTO THE COELIAC GANGLION OF THE CAT
     猫腹腔神经节注射HRP后脊神经节标记细胞的节段分布
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  标记的
     THE STUDY OF MEDMNH,M-C AND N_3S_3 LIGAND LABELLED WITH  ̄(67)Ga
      ̄(67)Ga标记的MEDMNH、M-C及N_3S_3的研究
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     Among 11090 labelled cells, the percentage ofeach labelled group was 72.2%, 26.3% and 1.5% respectively.
     在全部标记的11090个细胞中,每种标记细胞的百分数分别为72.1%,26.3%,1.5%.
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     The metabolically active form of vitamin D-1. 25 (OH)2D3 acted on the mouse fibroblasts L929 cells and then the changes of L929 cells nerve growth factor gene (NGF) mRNA in L929cells were detected with αP32labelled probe NGFcDNA.
     以维生素D的体内生理活性形式-1.25(OH)2D3作用于体外培养的小鼠成纤维细胞(L929细胞),然后用α-P32标记的神经生长因子(NGFcDNA)探针,检测L929细胞NGFmRNA的变化。
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     Methods Molecular hybridization was used with 32 p labelled hPV 16,18,c Ha ras 1,c myc DNA and DNA in cervical tissue showed by X ary film autography.
     方法:应用缺口翻译法32P—标记的hPV16,18,c-Ha-ras-1及c-myc-DNA与子宫颈组织中的DNA进行分子杂交,并通过X线胶片显示杂交结果。
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     COMPARATIVE FLOW CYTOMETRIC ANALYSIS OF CORD BLOOD CD34 POSITIVE CELLS LABELLED BY ANTI-HPCA-2-FITC AND TüK3
     脐血抗—HPCA—2和Tük3标记的CD34阳性细胞的流式细胞仪比较分析
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  “labelled”译为未确定词的双语例句
     SYNTHESIS AND FLUORESCENCE STUDY OF St/DVB COPOLYMERS LABELLED WITH PYRENE GROUPS
     SYNTHESIS AND FLUORESCENCE STUDY OF St/DVB COPOLYMERS LABELLED WITH PYRENE GROUPS
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     its average recovery was 99.81% while the average labelled content was 98.46% and RSD was 1.15%.
     平均回收率为 99.81% ,平均标示含量为 98.4 6 % ,RSD =1.15 %。
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     In this paper the labelled numbers f(x_(ij)) of the vertices x_(ij) for P_m×P_n graphs are given by (i=1,2,…,m;j=2,3,…,n) Where t=m(n-1)+n(m-1)+k-1. It is proved that P_m×P_n graphs are K-graceful.
     本文对 P_m×P_n 图的顶点 X_(ij)(i=1,2,…,m,j=1,2,…,n)作出标号:(i=1,2,…,m;j=2,3,…,n)式中,t=m(n—1)+n(m—1)+k—1。
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     If 3H 5 HTP or 3H 5 HT substituted for 3H try, labelled 5 HT, 5 HIAA, and 5 HIAA were recovered respectively.
     用3H-Try培养虫体,由HPLC分离收集,经液闪测定,有3H-5-HTP、3H-5-HT、3H-HIAA生成;
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     Three batches of eperison hydrochloride tablets were analyzed and the labelled contents were 97.8%,98.3% and 98.9% respectively.
     三批盐酸乙哌立松片含量测定结果分别为标示量的 97.8% ,98.3%和 98.9%。
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  labelled
To this end, we have chemically synthesized a fluorescein-labelled RNA primer and studied elongation of this primer by T7 RNA polymerase on a single-stranded DNA template.
      
We developed a fast and efficient screening method based on touchdown multiplex PCR with fluorescent labelled primers for the most common types of SCAs (SCA 1, 2, 3, and 7).
      
Among the products accumulated in the vacuole were the 14C-labelled sugars malate and alanine, small amounts of citric, glutamic, and aspartic acids, and some other amino acids.
      
Phosphorylationin vitro by cAPK showed a unique labelled band of approximately 60 ku, which was consistent with the molecular weight of the PKS-g3p fusion protein.
      
Theirin vitro phosphorylation analyses revealed the specific labelled bands corresponding to the positive PKS phages with and without the typical (R)RXS/T sequence motif.
      
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The LD_(50) of ammonium antimony gluconate (AAG) after intraperitoneal injection was 16.8mg/kg for mice fed on a diet containing 0.01% thyroxine, 166mg/kg for mice on a diet containing 0.1% propylthiouracil and 90mg/kg for control mice It is concluded that feeding mice with thyroxine for two weeks significantly increases the susceptibility of the mice to the acute toxicity of AAG, whereas propylthiouracil feeding increases the resistance of the mice. After intraperitoneal injection of Sb~(124)-labelled...

The LD_(50) of ammonium antimony gluconate (AAG) after intraperitoneal injection was 16.8mg/kg for mice fed on a diet containing 0.01% thyroxine, 166mg/kg for mice on a diet containing 0.1% propylthiouracil and 90mg/kg for control mice It is concluded that feeding mice with thyroxine for two weeks significantly increases the susceptibility of the mice to the acute toxicity of AAG, whereas propylthiouracil feeding increases the resistance of the mice. After intraperitoneal injection of Sb~(124)-labelled AAG to normal rats or rats fed thyroxine or propylthiouracil, high radioactivity levels were found in the liver and the gastrointestinal tract (including its contents), while the Sb~(124) levels in the kidney, thyroid, blood, spleen, lung and other tissues were very low. There seems to be no significant difference between the distribution patterns of radioactivity in tissues for the three groups of rats. The excretions of Sb~(124) for the three groups were also similar. It appears that the difference in toxicity of AAG between control rats and rats with altered thyroid function is not explicable by differences in the distribution and excretion of antimony after administration of AAG.

小鼠連續服用甲狀腺素兩週後,對銻銨急性毒性的敏感性顯著增加,而連續服用丙基硫氧嘧啶,則可增加對銻銨急性毒性的耐受性。腹腔注射銻銨於甲狀腺機能亢進狀態,甲狀腺機能不足及正常大鼠後,銻~(124)在组織中分佈的濃度均以肝及胃腸道为最高,腎臟次之,在甲狀腺中的濃度均很低。腹腔注射銻”銨後,銻~(124)的排泄總量三组動物無明顯區别。因此,銻銨對甲狀腺機能亢進狀態,甲狀腺機能不足及正常小鼠毒性的不同,似並非由於注射銻銨後,銻的分佈和排泄不同所致。

Chloroplasts, prepared from fresh, prechilled spinach leaves by the method of Jagendorf andAvron, were suspended in Tris buffer (25 μmoles, pH 8.4) containing MgCl_2 (6 μmoles), ADP(3 μmoles), P~(32)-labelled phosphate (3 μmoles), NaCl (25 μmoles) and the different cofactors. Totalvolume of the reaction mixture was 1.25 ml, containing 30-40 μg chlorophyll. Preliminary experi-ments showed that at the low illumination intensity for quantum yield determinations the optimal quan-ties of the co-factors were:...

Chloroplasts, prepared from fresh, prechilled spinach leaves by the method of Jagendorf andAvron, were suspended in Tris buffer (25 μmoles, pH 8.4) containing MgCl_2 (6 μmoles), ADP(3 μmoles), P~(32)-labelled phosphate (3 μmoles), NaCl (25 μmoles) and the different cofactors. Totalvolume of the reaction mixture was 1.25 ml, containing 30-40 μg chlorophyll. Preliminary experi-ments showed that at the low illumination intensity for quantum yield determinations the optimal quan-ties of the co-factors were: PMS or FMN, 0.005 μmole, vitamin K_3, 0.03 μmole, and of the Hilloxidants were: K_3Fe(CN)_6 0.6 μmole, TPN 0.2 μmole. Neon tube with dilute ammoniacal CuSO_4 solution and red-glass filters was used as light source.The wave length range was 620-660 mμ and the intensity was 6-8×10~3 ergs/cm~2/sec. Energydeterminations were made with a blackened constantin-copper thermopile, the absolute energy wascalibrated by the amount of heat produced electrically at the surface of the thermopile. The calcu-lated number of quanta was counter-checked by chlorophyllide actinometer according to Warburg.Light scattering was corrected by the ground glass method of Shibata. Phosphorylation rates weremeasured by ATP~(32) formed according to the method of Nielsen and Lehninger. No incorporation of P~32 was detectable in the dark. To avoid loss of activity preparations were conducted near 0℃ and experiments were completedwithin 10 minutes including isolation of chloroplasts. Representative results are given in Tab. 1. The following conclusions can be drawn. (1) The quantum requirement of cyclic photophosphorylation is between 2.9-6.5 (generally4-5) per molecule of ATP formed, irrespective of the co-factors used,. Although at high lightintensities PMS can be twice as active as vitamin K_3 or FMN, their quantum yields are the same. (2) Same numerical results are obtained with heat deproteinized leaf extract in place of co-factors. (Tab. 3). (3) Non-cyclic photophosphorylation with K_3Fe(CN)_6 or with TPN shows the same quantumrequirement of 4-6. (Tab.1). Apparently one and the same electron transport system is involvedin both types of phosphorylation and there exists probably only one phosphorylation site. However,the possibility of a second easily inactivated site is not excluded. (4) The simultaneously measured Hill reaction requires 9-12 quanta per molecule of O_2evolved with or without photophosphorylation (+ or -ADP, Tab.2). The result corroboratesthose of other workers. It further shows that under the conditions of the present experiment cou-pling is complete (P/2e = 1), and that no extra quantum is needed for the coupled formation ofATP. (5) The quantum requirement of both cyclic and noncyclic phosphorylation increased withdecreasing light intensity within the range used (1.3-6.0×103 ergs/cm~2/sec.) (Tab. 4), whereasthat of O_2 production by the Hill reaction remains constant, thus resulting in a progressive uncou-pling of phosphorylation from the electron transport chain. The relationship between the present results and the quantum requirement of photosyntheticCO_2-reduction is discussed. That at low intensities of illumination used in the present experimentsthe number of quanta required for 2 TPNH and 2 ATP formation equals to that of photosynthesisunder these conditions (~compensation point) indicated that cyclic production of ATP is perhapsnot involved and the extra ATP needed for CO_2-reduction in the Calvin cycle must come fromsome other source, e.g. respiration. That at higher light intensities (several times compensationpoint) the quantum requirement of photosynthesis increases is probably due to the coming into playof the photochemically inefficient cyclic photophosphorylation.

用菠菜叶绿体悬浮液,在红光下(620—660mμ,6—8×10~3尔格/厘米~2-秒)测定同位素P~(32)标记的无机磷酸进入ATP的强度,并根据吸收的光能量换算为形成一个分子ATP所需要的红光量子数。结果指出: (1)循环光合磷酸化作用,不论用何种辅助因素(PMS,维生素K_3,FMN),形成一个分子ATP的量子需要量均在4—5之间(最低一次获得2.9)。叶提取液代替辅助因素,结果亦同。(2)与希尔反应偶联的光合磷酸化作用(希尔氧化剂为K_3Fe(CN)_6或TPN)的量子需要量亦是4—6。同时测定的还原作用指出希尔反应中每放出一个分子O_2,需要8—12个红光量子,表示在试验条件下,二者是完全偶联的(P/2e?1)。没有磷酸化(不加ADP及P_i)时,希尔反应的量子需要量不变,表示偶联的ATP形成不需额外的光量子。(3)光强度减低,则循环与非循环光合磷酸化作用的效率随之降低,量子需要量增加,而希尔反应的效率则不变。从上述结果推论,两种光合磷酸化作用均是通过同一的电子传递系统,在此系统中仅有一个磷酸化部位,除非另有一个部位是极易破坏的。试验结果也对光合作用的量子需要量问题,供给可能的解释。在弱光下光合作用效...

用菠菜叶绿体悬浮液,在红光下(620—660mμ,6—8×10~3尔格/厘米~2-秒)测定同位素P~(32)标记的无机磷酸进入ATP的强度,并根据吸收的光能量换算为形成一个分子ATP所需要的红光量子数。结果指出: (1)循环光合磷酸化作用,不论用何种辅助因素(PMS,维生素K_3,FMN),形成一个分子ATP的量子需要量均在4—5之间(最低一次获得2.9)。叶提取液代替辅助因素,结果亦同。(2)与希尔反应偶联的光合磷酸化作用(希尔氧化剂为K_3Fe(CN)_6或TPN)的量子需要量亦是4—6。同时测定的还原作用指出希尔反应中每放出一个分子O_2,需要8—12个红光量子,表示在试验条件下,二者是完全偶联的(P/2e?1)。没有磷酸化(不加ADP及P_i)时,希尔反应的量子需要量不变,表示偶联的ATP形成不需额外的光量子。(3)光强度减低,则循环与非循环光合磷酸化作用的效率随之降低,量子需要量增加,而希尔反应的效率则不变。从上述结果推论,两种光合磷酸化作用均是通过同一的电子传递系统,在此系统中仅有一个磷酸化部位,除非另有一个部位是极易破坏的。试验结果也对光合作用的量子需要量问题,供给可能的解释。在弱光下光合作用效率高,可能是由于部份ATP来自呼吸;而在强光下效率减低,则是呼吸所供给的ATP不足而必需依靠循环光合磷酸化所致。

The effects of the total alkaloids isolated from the root of Rauwolfia verticillata from Hainandao on the circulatory and organ plasma and cell volumes were studied by using I~(131)-labelled serum protein and P~(32)-labelled red blood cells. It was found that: (1) Intravenous injections of the alkaloids 5mg/kg to normal or renal hypertensive rats produced no remarkable change in the circulatory plasma and cell volumes. (2) The alkaloids could dilate the pulmonary vessels in normal rats. It also caused...

The effects of the total alkaloids isolated from the root of Rauwolfia verticillata from Hainandao on the circulatory and organ plasma and cell volumes were studied by using I~(131)-labelled serum protein and P~(32)-labelled red blood cells. It was found that: (1) Intravenous injections of the alkaloids 5mg/kg to normal or renal hypertensive rats produced no remarkable change in the circulatory plasma and cell volumes. (2) The alkaloids could dilate the pulmonary vessels in normal rats. It also caused a relaxation of the hypertonic vessels of the myocardium, kidmey and liver of the renal hypertensive rats. (3) The specific radioactivities of the liver, spleen and kidney of mice were decreased 1/2 hour after an intragastric administration of the alkaloids 100 mg/kg. The counting rate of the lungs was increased after 1(1/2) hours, indicating a pulmonary vasodilatation. (4) Intraperitoneal injection of the alkaloids in rats did not bring about a significant alteration in the vascular permeability in 3 hours. Thus the change in the specific radioactivity of the organs should be explained chiefly by the local changes in the vascular dimensions.

用I~(131)标記血清蛋白及P~(32)标記紅血球研究海南島蘿芙木根硷对循环及器官血浆及血球容量的影响,发見: (一)正常或腎型高血压大白鼠靜脉注射此硷5毫克/公斤,5分鉀后,循环血浆及血球量均无明显影响。 (二)此硷能使正常大白鼠肺血管扩张,使腎型高血压大白鼠心肌、腎、肝等器官的缺血状态得到改善。 (三)小白鼠灌胃此硷100毫克/公斤,30分鉀后肝、脾及腎的比放射性减低,90分钟后肺的計数率則增高,提示此硷可能扩张肺血管。 (四)大白鼠腹腔注射此硷180分鉀內血管通透性无改变,因此訊为此时器官比放射性的变化应該主要用血管本身的舒縮和充盈的改变来解释。

 
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