3 715 resected lymph nodes (LN ) contained no metastatic tumor by conventional histopathological examination from 350 patients with different early carcinomas including non - small cell lung carcinoma (NSCLC) 94, breast carcinoma 112, esophageal carcinoma 115, and vulvar carcinoma 29, were re-examined by immunohistochemistry.
Methods A case of hepatosplenic γδT-cell lymphoma was stud ied with conventional histopathological and immunohisto-chemical staining in com bination of literature review.
Method A case of primary cardiac large B-cell lymphoma was studied with conventional histopathological and immunohistochemical staining in combination with literature review.
Methods The conventional histopathological, Gleason grading, and P504s, p63 and 34βE12 immunohistochemistry(SP method) were applied in 71 cases of prostate adenocarcinoma.
Methods Autopsy of two cases of radiation sickness was performed and conventional histopathological sections of lung tissue were prepared,which were stained immunohistochemically for TGF_β,α-SMA,precollagen Ⅳ,MMP-9 and Sirius red.
Conventional histopathological me thod was utilized to observe morpbological changes and cell counting of dentate granule cells, CA 3,CA 1 and hilar neurnns.
Methods Paraffin section of 135 hydatidiform moles and 13 normal chorion were studied with conventional histopathological and immunohistochemical stains to detect the proliferation of trophoblast and the expression of EGFR.
The biological behavior of meningiomas often can not be predicted from conventional histopathological studies.
Furthermore, we tried to compare the RT-PCR results with those obtained by conventional histopathological examination.
Though both factors might amplify the prognostic information for distinct patient subsets they do not achieve the strong prognostic value of conventional histopathological features in breast cancer.
Expression of proliferating cell nuclear antigen (PCNA) and c-erbB-2 oncoprotein has been assessed in 471 women with breast cancer to evaluate their prognostic value as compared to conventional histopathological factors.
Prognostic value of proliferating cell nuclear antigen and c-erbB-2 compared with conventional histopathological factors in brea
We re-examined 1840 regional lymph nodes which documental no tnmor cells by conventional histopathological examination from 112 female patients with stage I breast cancer using immunohistochemistry LSAB techniques.Monoclonal anticytokeratins(AE1/AE3),anti-EMA,and polyclonal anti-keratins antibodies were used as the primary reagents to identify tumor cells both in tumor tissues and lymph nodes.Over all, immunostaining positive tumor cells were found in 27.7% of lymph nodes of patients(31/112) and 2.27%(50/1840)of...
We re-examined 1840 regional lymph nodes which documental no tnmor cells by conventional histopathological examination from 112 female patients with stage I breast cancer using immunohistochemistry LSAB techniques.Monoclonal anticytokeratins(AE1/AE3),anti-EMA,and polyclonal anti-keratins antibodies were used as the primary reagents to identify tumor cells both in tumor tissues and lymph nodes.Over all, immunostaining positive tumor cells were found in 27.7% of lymph nodes of patients(31/112) and 2.27%(50/1840)of the dissected lymph nodes.Seven years postoperative follow-up observation revealed that the incidence of recurrence was high and the latent period of distant metastases was much shorter in patients with occult micrometastases than those without.
3 715 resected lymph nodes (LN ) contained no metastatic tumor by conventional histopathological examination from 350 patients with different early carcinomas including non - small cell lung carcinoma (NSCLC) 94, breast carcinoma 112, esophageal carcinoma 115, and vulvar carcinoma 29, were re-examined by immunohistochemistry. The results showed:in 56. 4% NSCLC patients (53/94) and 16. 6% LN (123/739) ,27. 7% breast cancer patients (31/112) and 2.72% LN (50/1 840),22. 6% esophsgeal cancer patients (26/115)...
3 715 resected lymph nodes (LN ) contained no metastatic tumor by conventional histopathological examination from 350 patients with different early carcinomas including non - small cell lung carcinoma (NSCLC) 94, breast carcinoma 112, esophageal carcinoma 115, and vulvar carcinoma 29, were re-examined by immunohistochemistry. The results showed:in 56. 4% NSCLC patients (53/94) and 16. 6% LN (123/739) ,27. 7% breast cancer patients (31/112) and 2.72% LN (50/1 840),22. 6% esophsgeal cancer patients (26/115) and 7.0% LN (27/381),and 10. 3% vulvar cancer patients (3/29) and 1.4% LN (3/216), and 32. 3% all cancer patients (113/350) and 5. 5% all examined LN (203/3 715)) micro metastases and/or single cancer cell were found. The incidence of immunostaining positive tumor cells in LN in squamous cell carcinoma (58. 0%),adenocarcinoma (53. 8%) of NSCLC patients was higher than those in esophageal and vulvar squamous cell carcinoma and breast adenocarcinoma (P<0. 005). The frequency in LN was a similar patterns. The data suggested that combined consecutive sections and immunostaining will greatly increase the yield of occult micrometastases in resected LN; the micrometastatic tumor may be the primary step for the further wild dissemination of malignant cells; The rapid metastases and high death rate of lung cancer may be related to,at least partial,the occult metastases;the involved LN were resected as can as possible at surgical therapy as a preventive tumor dissemination will have good outcome.
Objective:Bax gene is an important apoptosispromoting gene. ln order to investigate the expression of Bax in oral premalignant lesions and oral squamous cell carcinomas, a total of 38 samples are evaluated using a labelled streptavidin biotin (LSAB) immunohistochemical assay. Methods: A total of 38 specimens were studied, including normal oral mucosa, premalignant lesions and squamous cell carcinomas. The specimens were obtained and blocked, fixed with 10% buffered formalin and embedded in paraffin using conventional...
Objective:Bax gene is an important apoptosispromoting gene. ln order to investigate the expression of Bax in oral premalignant lesions and oral squamous cell carcinomas, a total of 38 samples are evaluated using a labelled streptavidin biotin (LSAB) immunohistochemical assay. Methods: A total of 38 specimens were studied, including normal oral mucosa, premalignant lesions and squamous cell carcinomas. The specimens were obtained and blocked, fixed with 10% buffered formalin and embedded in paraffin using conventional histopathological techniques. 3 μm thick sections of paraffin embedded tissues were cut, mounted onto slides coated with 5% APES (3 aminopropyltriethoxysilane), dried overnight at 56℃, dewaxed in xylene and rehydrated through descending graded alcohols to phosphate buffered saline (PBS, pH 7.4). For antigen retrieval, slides were immersed in 10 mmol/L sodium citrate buffer (pH 6 0) and boiled twice for 5min in a microwave oven (800 W). After treated for 10 min with 3% H\-2O\-2, 18% methanol in PBS, the slides were covered with 10% normal porcine serum for 10 min at 37℃. Then slides were incubated with primary antibody (bax rabbit polyclonal antibody) for 60 min at 37℃ and were subsequently incubated with prediluted biotinylated antibody against rabbit immunogobulins, and streptavidinhorseradish peroxidase conjugate for 30 min at 37℃. After washing, peroxidase activity was detected using 3,3' diaminobenzidine as chromogen with H\-2O\-2 as substrate. The cells in the test specimens which demonstrated granular staining were considered as positive. A haemocytometer counter with 6\+*6 framework were applied and only the positive cells on the cross were counted. The cell counting were processed in ten randomly chosen 400\+* microscopic fields and the mathematic mean was presented as the final counting of each sample. Statistical valuations were performed using the version 6.0 SPSS package. Positive controls were sections of bladder cancer tissues. Blank controls were fabricated for each specimen by the omission of the primary antibody, which was replaced with PBS.\ Results: In the process of oral carcinogenesis, each stage had Bax expression. The positive staining appeared in cytoplasm.\ In the normal oral mucosal specimens Bax expression was evident in the prickle layer, but not in the basal cell layers. Various degrees of Bax expression were seen in the diseased tissues. The staining pattern of hyperkeratotic lesions was similar to the normal oral mucosa, but Bax expression were also seen in the basal cell layer.\ In the mild, moderate, severe dysplasia and squamous cell carcinomas, Bax expression were seen in all layers, however, the intensity of staining were greater in mild and moderate dysplasia. The number of positive cells tended to increase gradually with the development of cell maligancy in the tissues of hyperkeratosis, mild and moderate dysplasia (P<0 05). In the tissue of squamous cell carcinomas the number of positive cells had no marked difference comparing with the normal oral mucosa. Conclusion: The expression of Bax is involved in oral carcinogenesis and the compensative increase of Bax protein expression may be an early response.
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