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method and condition
相关语句
  方法和条件
    The study of isolation method and condition of brophytes total DNA
    苔藓植物总DNA提取方法和条件的研究
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  方法及条件
    Research on the Precipitation Method and Condition of Extracting Pectin from Pomelo Peel
    果胶提取中沉淀方法及条件的研究
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  “method and condition”译为未确定词的双语例句
    5. Chironomus was given a classification by molecular classification. The total DNA was extracted from four different species of Chironomus and used as template to amplify the 18s rRNA ITS1 by PCR and then the 18s rRNA was analyszed by enzyme. The method and condition of molecular classification of Chironomus was summzrized.
    5.尝试了对摇蚊分子分类的研究,通过对4个不同种类摇蚊的DNA提取、PCR扩增和酶切分析,总结出摇蚊分子分类的条件和方法。
短句来源
    To investigate insect chitosan basic characteristics and differences between insect chitosan and Procambarus clarkii chitosan,studies were done on ash,deacetylation degree,molecular weight,infrared spectrums of chitosans which distilled from shell of P.clarkii、pupa shell of Musca domestica、shell of Euplyphaga Walker、exuviate of Tnebrio molitor L.in the same method and condition in this paper.
    为了了解昆虫来源几丁糖的基本特性及其与河虾来源几丁糖的差别,用同一方法在相同条件下分别以河虾壳(shell of Procambarus clarkii)、家蝇蛹壳(pupa shell ofMusca domestica vicinaMacquart)、地鳖虫壳(shell of Euplyphaga Walker)和黄粉虫蜕(exuviate of Tnebrio molitorL.) 为来源制备的几丁糖在灰份、脱乙酰度、分子质量等及红外图谱上进行比较研究。
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AIM To establish a new model of isolation andprimary culture in vitro for further study. METHODS The stomachs of rats were reverted to form sacs with the mucosa facing outward and serosa facing inward. Pronase E solution was injected into the sac lumina. The stomachs were incubated in 950 mL·L -1 O 2 and 50 mL·L -1 CO 2 at 37℃for 60 min, then stirred at room temperature and the dispersed cells were collected to be loaded on a density gradient (40%~60% per cell) centrifugation. For removal...

AIM To establish a new model of isolation andprimary culture in vitro for further study. METHODS The stomachs of rats were reverted to form sacs with the mucosa facing outward and serosa facing inward. Pronase E solution was injected into the sac lumina. The stomachs were incubated in 950 mL·L -1 O 2 and 50 mL·L -1 CO 2 at 37℃for 60 min, then stirred at room temperature and the dispersed cells were collected to be loaded on a density gradient (40%~60% per cell) centrifugation. For removal of fibroblasts, the cells were suspended in PSS containing 100 mL·L -1 fetal bovine serum FBS and preattached in flasks coated with rat tail glue at 37℃ for 30 min. The rich parietal cells were maintained in 1∶1 Ham's F 12/DMEM contained insulin 5 mg·L -1 , hydrocortaison 4 μg·L -1 , tranferrin 5 mg·L -1 , sodium selenite 5 μg·L -1 , BSA 2 g·L -1 , epidermal growth factor (EGF) 25 μg·L -1 , D glucose 1.98 g·L -1 . They were cultured continuously in serum free media and survived for over one week. RESULTS Identified by acridine orange (AO), the purity of isolated and cultured rat gastric parietal cells was over 80% and they grew very well. CONCLUSION Our methods and condition are suitable for the isolation and primary culture of rat gastric parietal cells.

目的 完成胃壁细胞分离及纯化 ,建立原代培养胃壁细胞的方法 .方法 制备大鼠翻转的胃囊用含链霉蛋白酶E的消化液注入胃囊内孵育 ,并用磁力搅拌器轻轻搅拌而制备单个的胃底腺细胞 ,Percoll梯度离心富集胃壁细胞 ,用含10 0 m L· L- 1 血清的 PBS或培养液 ,时差贴壁去除成纤维细胞 ,然后 ,将壁细胞接种于培养板中 ,培养液用无血清的 1∶ 1Harm's F- 12 / DMEM培养 ,内含胰岛素 5 mg· L- 1 ,氢化可地松 4μg· L- 1 ,转铁蛋白 5 mg· L- 1 ,硒酸钠 5μg· L- 1 ,牛血清白蛋白 2 g· L- 1 ,表皮生长因子 2 5 μg· L- 1 ,葡萄糖 1.98g· L- 1持续培养可达 1wk以上 .结果 获得的壁细胞经吖啶橙 (acridine orange,AO)鉴定纯度达 80 %以上 ,原代培养经HE染色可见壁细胞呈分散小组式生长 ,且生长状态良好 .结论 此方法适宜于大鼠胃壁细胞的分离及体外培养 ,为体外进一步研究壁细胞的功能打下基础 .

To investigate insect chitosan basic characteristics and differences between insect chitosan and Procambarus clarkii chitosan,studies were done on ash,deacetylation degree,molecular weight,infrared spectrums of chitosans which distilled from shell of P.clarkii、pupa shell of Musca domestica、shell of Euplyphaga Walker、exuviate of Tnebrio molitor L.in the same method and condition in this paper.On ash,insect chitosan was lower than shell of P.clarkii.At molecular weight,pupa shell of M.domestica chitosan...

To investigate insect chitosan basic characteristics and differences between insect chitosan and Procambarus clarkii chitosan,studies were done on ash,deacetylation degree,molecular weight,infrared spectrums of chitosans which distilled from shell of P.clarkii、pupa shell of Musca domestica、shell of Euplyphaga Walker、exuviate of Tnebrio molitor L.in the same method and condition in this paper.On ash,insect chitosan was lower than shell of P.clarkii.At molecular weight,pupa shell of M.domestica chitosan was obviously lower than other three kinds.In deacetylation degree,pupa shell of M.domestica and shell of Euplyphaga chitosan was relatively higher than others.their Infrared spectrums were basically identical.

为了了解昆虫来源几丁糖的基本特性及其与河虾来源几丁糖的差别,用同一方法在相同条件下分别以河虾壳(shell of Procambarus clarkii)、家蝇蛹壳(pupa shell ofMusca domestica vicinaMacquart)、地鳖虫壳(shell of Euplyphaga Walker)和黄粉虫蜕(exuviate of Tnebrio molitorL.)为来源制备的几丁糖在灰份、脱乙酰度、分子质量等及红外图谱上进行比较研究。昆虫来源几丁糖,其灰份低于河虾壳来源几丁糖;家蝇蛹壳几丁糖分子质量明显低于其他3种来源的几丁糖;从几丁糖质量角度看,采用家蝇蛹壳和地鳖虫壳可制备脱乙酰度较高的几丁糖;4种来源的几丁糖红外光谱图谱基本一致,具有几丁糖的特征吸收峰。

Objective To study the methods and condition on preservation and recovery of Bartonella spp.Methods The strains were selected to test for Bartonella preservation with reproductive culture and freezing methods.Results Strains could be preserved with two methods and strains grew well after recovering from-70℃ and-196℃.Conclusions Two methods are effective for strains preservation.Bartonella spp.is highly resistant to freezing and freeze-thaw cycles.

目的探索巴尔通体菌株保存和复苏的方法及其条件。方法应用传代持续培养保存法和冷冻保藏法对所保存菌株进行实验,并对冷冻保藏后的菌种进行复苏试验。结果两种方法能较好地保存巴尔通体菌株,复苏后生长良好。结论两种方法保存菌种是可行的。巴尔通体菌株对冷冻和反复冻融有很强的抵抗力。

 
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