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method using     
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  方法应用
     Methods Real-time quantitative PCR method using SYBR Green I technology was taken to measure the effects of Cpd861 on expression of α-SMA mRNA in LX-2 cells treated by Cpd861 for 24, 48 and 72 h.
     方法应用基于SYBR-Green I的实时定量荧光PCR方法,研究复方861分别作用24、48、72 h后,LX-2细胞中α-SMA mRNA含量的变化情况。
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     Method Using PCR/SSCP and DNA sequencing, we studied the mutations in exons 3, 5, 7, 10, 11, 12 of PAH gene. Totally 120 unrelated children with PKU and their parents from the northern region of China were included in the analysis.
     方法 应用PCR/SSCP、序列分析等技术,分析中国北方地区120个经典型苯酮尿症(PKU)核心家系,对PAH基因外显子3、5、7、10、11、12进行突变基因的筛查和确定。
短句来源
     Method Using reverse transcription polymerase chain (RT-PCR) mRNA overexpression of Tiam1 gene in 30 cases of carcinoma of larynx tissue,12 lymph nodes and 10 cases of normal larynx tissue was studied.
     方法应用逆转录聚合酶链反应(RT-PCR)检测30例喉癌组织、12例喉癌转移的颈部淋巴结和10例正常喉部组织Tiam1mRNA的表达。
短句来源
     Method Using PCR,to give a HSV Ⅰ DNA inspection to the cornea of 20 pure New Zealand rabbits which have infected with HSK and 60 patients who also infected wich HSK.
     方法应用多聚酶链反应(PolymaraseChainReaction,PCR)对感染HSK的20只纯新西兰白兔和60例HSK患者的角膜进行了单疱病毒-Ⅰ(herpessimplexvirus-I,HSV-I)DNA检测。
短句来源
     Methods HGV antigen was detected with immunohistochemical method using moloclonal mouse antibody to HGV NS5 in 142 patients with chronic liver disease, and serum HGV RNA was detected by RT-nested PCR in some of the subjects.
     方法应用免疫组织化学方法以鼠抗HGVNS5甲克隆抗体检测142例慢性肝病患者肝组织中HGV抗原,部分患者采用RT-PCR方法检测其血清中HCVRNA。
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  法用
     Lung tissue myeloperoxidase enzyme (MPO) activities reflecting total lung neutrophil infiltration was assessed by modified Bradly's method using H2O2 as a substrate .
     Bradly改良法用H2O2作为反应底物测定髓过氧化物酶(MPO)的活性,用以反映肺组织总的中性粒细胞浸润数量。
短句来源
     Method:The expression of macrophages and MVD in 102 cases of gastric carcinoma were investigated by immunohistochemical SP method using monoclonal antibody to CD68 and CD34. The area of neovascularization was assessed quantitatively by computer-assisted video analysis system.
     方法:应用免疫组化SP法,用抗CD68 和抗CD34单克隆抗体对102例胃癌组织进行标记分析,并应用计算机辅助图像分析系统对血管新生的面积进行定量分析。
短句来源
     Methods The microvessel density(MVD)was determined by immunohistochemical SP method using a monoclonal antibody to CD 34 in samples of 40 gastric carcinoma and 10 normal gastric tissue.
     方法 采用免疫组织化学SP法 ,用抗血管内皮CD34单克隆抗体对 40例胃癌及 10份正常胃组织标本进行标记分析。
短句来源
     Method The MMP-9 expression and the change of MVD of 40 specimens resected from patients with primary gastric carcinoma were investigated by immunohistochemical SP method using monoclonal antibody to MMP 9 and CD34 . Results 29 out of the 40 cases were MMP 9 positive(72.5%), The overexpression of MMP 9 was correlated to lymph node metastasis and poorer differentiation( P <0.01 and P <0.05, respectively);
     方法 采用免疫组化SP法 ,用抗MMP - 9及抗血管内皮细胞CD34单克隆抗体对 40例胃癌组织标本进行标记分析。 结果 弥漫型胃癌、有淋巴结转移组MMP 9阳性表达率分别明显高于肠型胃癌和无淋巴结转移组 (P值分别 <0 .0 5和 0 .0 1 ) ;
短句来源
     Methods:The MMP-9 expression and MVD of 44 specimens resected from patients with colorectal carcinoma were investigated by immunohistochemical SP method using monoclonal antibody to MMP-9 and Factor Ⅷ and Anti-collagen Ⅳ?
     方法 :采用免疫组化SP法 ,用MMP - 9抗体标记 4 4例结肠癌组织 ,用第Ⅷ因子抗体标记组织内的血管 ,计数微血管密度 ,用Ⅳ型胶原抗体标记细胞外基质和基底膜。
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  方法运用
     Method:Using techniques of tumor cells culture in vitro, the suppressive and time dependency effects of taxol (1×10 -8 mol/L) combine 2 Gy X rays generated by accelerator on the human laryngeal squamous cell carcinoma Hep 2 cell lines were observed.
     方法 :运用体外肿瘤细胞培养技术 ,观察 1× 10 -8mol/L紫杉醇联合直线加速器产生的 2GyX 线对人喉鳞癌细胞株Hep 2的生长抑制作用及时间效应。
短句来源
     The expressions of collagen Ⅳ α chains on the epidermal basement membrane(EBM)of the skin,the glomerular basement membrane (GBM),distal tubular basement membrane and Bowman′s capsule of the kidney were detected by indirect immunofluorescent(IF)method using monoclonal antibodies against α1,3 and 5 chains of collagen Ⅳ.
     方法 :运用针对Ⅳ型胶原不同α链单抗 ,采用间接免疫荧光法(iIF)检测皮肤和肾组织基膜Ⅳ型胶原α1、3、5链分布。
短句来源
     Method:Using twelve-lead ECGs to measure the QT dispersion and its corrected values(QTd and QTcd)in 118 cases with or without hypertrophic ventricle.
     方法 :运用同步十二导联心电图机检测114例有或无心室肥厚患者的QT离散度及校正值(QTd、QTcd)。
短句来源
     As a business analysis method,application of data mining technology on analysis of dispatching data network business flow is different form the method using data package as a unit. This analysis method can analyze network situation and network behavior using business perspective,for the purpose of reaching the goal of network business flow design.
     且指出 ,在网络业务流分析中采用数据挖掘技术是一种业务级的分析方法 ,不同于以数据包为单位的方法 ,运用该方法可从业务角度对网络状况和网络行为进行分析 ,从而达到网络业务流设计的目标
短句来源
     Method Using the bedside technique developed by Ross, revised by Hughes, we examined affective prosody and the production of Chinese tones in 39 Chinese patients with single right hemisphere infarction (RHI) confirmed by CT or MRI and in 40 normal controls (NC) matched in age, sex, and literacy.
     方法运用Ross创建的床边检查法并参照Hughes等的方式加以修订,包括情感性语韵和汉语声调产生的测试检查39例首发单灶右半球梗死病人和40例年龄、性别、文化匹配的正常对照。
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  方法借鉴
     As teaching process is a complex course, how to recharge old teaching method, using the teaching methods of other subjects, to create new teaching method and reaching style in the background of new curriculum to get physical education more effectively and practically is the question the author wants to discuss and describe as below.
     教学过程是一个复杂的过程,在新课程的背景下,就如何改造旧的教学方法,借鉴其它学科的教学方法创造新的教学方法和教学方式,使新课程标准下体育教学高效、实效等问题进行了探讨。
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The proof is based on a variant of Moser's method using time-dependent vector fields.
      
Tungsten trioxide powder has been prepared from ammonium paratungstate via hydrothermal method using orthogonal and mono-level design of experiments.
      
In this paper, perovskite oxide SmCoO3 was prepared by the solid-state reaction method using Co2O3 and Sm2O3 as raw materials.
      
A new feature extraction method using the amplitude fluctuation property of target HRRP for radar automatic target recognition
      
Based on the scattering center model, a new feature extraction method using the amplitude fluctuation property of target HRRP is proposed in this paper.
      
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A continuous method using mixing column and aeration column with yield over 90% for the preparation of ethylorthosilicate was described.

本文敍述使用混合塔及通氣塔裝置的連續法製備正矽酸乙酯。收率超過90%。

During the investigation on the isolation of aureomycin from fermentation broth, it was observed that the stability of aureomycin depended upon the pH and temperature of the solution. The present report deals with the influence of pH and temperature on the stability of the antibiotic.The destruction of aureomycin in acid solution was followed by measuring colorimetrically anhydroaureomycin formed during the experimental period. The optical density of the developed color in dilute hydrochloric acid is proportional...

During the investigation on the isolation of aureomycin from fermentation broth, it was observed that the stability of aureomycin depended upon the pH and temperature of the solution. The present report deals with the influence of pH and temperature on the stability of the antibiotic.The destruction of aureomycin in acid solution was followed by measuring colorimetrically anhydroaureomycin formed during the experimental period. The optical density of the developed color in dilute hydrochloric acid is proportional to the concentration of aureomycin. The concentration of anhydroaureomycin is also proportional to the optical density of the color.In alkaline medium, aureomycin is rearranged into iso-aureomycin. The amount of the remaining antibiotic, which was determined by converting to anhydroaureomycin, served as a measure for the stability. For check we have also measured the aureomycin left undestroyed with the help of a serial dilution method using Bacillus subtilis as test organism. This is possible since the bacteriostatic activity of anhydroaureomycin is only about one-twentieth of that of the antibiotic and isoaureomycin has no bacteriostatic activity.The results are presented in the tables below:

本文测定了金黴素在酸性溶液中(0.01,0.1及1N鹽酸)及在鹼性緩衝液中(pH7.4,8.0,8.6,9.2)的穩定性,找出在鹼性緩衝液中的穩定性較在酸性溶液中为小。穩定性随温度增加而降低。並敍述了經酸或鹼處理後溶液中金黴素含量的测定方法。

Eight common alkaloidal poisons:Strychnine-Brucine;Cocaine-Atropine;Quinine-Nicotine;& Morphine-Codeine have been separated satisfactorily from each other by circular chromatographic method using domestic filter paper.The method is rather simple and rapid and the separation is as effective as that obtained with Whatman No.1 filter paper.The paper is treated with 5% Sodium dihydrogen citrate,using n-butanol,glacial acetic acid and water(100:30:70)as the deve- loping solvents.The average Rfc...

Eight common alkaloidal poisons:Strychnine-Brucine;Cocaine-Atropine;Quinine-Nicotine;& Morphine-Codeine have been separated satisfactorily from each other by circular chromatographic method using domestic filter paper.The method is rather simple and rapid and the separation is as effective as that obtained with Whatman No.1 filter paper.The paper is treated with 5% Sodium dihydrogen citrate,using n-butanol,glacial acetic acid and water(100:30:70)as the deve- loping solvents.The average Rfc values are shown in the following figures: Strychnine…………0.58 Quinine…………0.85 Brucine…………0.69 Nicotine…………0.37 Cocaine…………0.84 Morphine…………0.50 Atropine…………0.79 Codeine…………0.54

本文采用灯芯法纸层析,以正丁醇、冰醋酸、水以100:30:70作展开溶剂,进行生物硷类毒物的分离览别工作,得到初步结果,并用“白雪”牌“国一号”定性滤纸与 Whatman No.I滤纸作了比较,证明国产滤纸可以代替 Whatman No.I 滤纸,并得到满意的结果.灯芯法分离生物硷类毒物所需之时间远比上渗法或下降法短,且可得到同样结果在与对照同时展开时可以分别检出未知生物硷类毒物.

 
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