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osteocalcin of cells
相关语句
  骨钙素分泌
     The leptin stimulated synthesis of osteocalcin of cells was found to be dose dependent ( P <0.05), but not time dependent ( P >0.05).
     L eptin可刺激骨钙素分泌 ,呈浓度依赖性 (P<0 .0 5 ) ,但无时间依赖性 (P>0 .0 5 )。
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  相似匹配句对
     ⑦ assay of osteocalcin.
     ⑦骨钙素的测定结果。
短句来源
     Osteocalcin and children's growth and development
     骨钙素与儿童生长发育
短句来源
     RADIOIMMUNOASSAY AND CIJNICAL SIGNIFICANCE OF OSTEOCALCIN
     骨钙素放射免疫测定方法及其临床意义
短句来源
     Study on the enzyme immunoassay for osteocalcin in serum
     骨钙素酶免疫检测方法的研究和应用
短句来源
     The induced cells still expressed osteocalcin and collagen Ιin vivo.
     诱导细胞与材料复合后植入体内仍然高表达骨钙素、Ⅰ型胶原。
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Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining,...

Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining, and positive by Alizarin Reds staining after mineralized upon supplementation with ascorbate and β glycerophosphate. On the first, second and third days, the prolifertion of osteoblast, cultured with different concentrations of leptin, had no changes. The leptin stimulated synthesis of osteocalcin of cells was found to be dose dependent ( P <0.05), but not time dependent ( P >0.05). Conclusion The above data indicated that there were no evidences for the effects of leptin on the proliferation of human osteoblast, but leptin could enhance the function of human osteoblast.

目的 观察瘦素 (leptin)对人成骨细胞增殖和功能的影响。方法 培养人成骨细胞 ,并观察其形态及功能变化。采用 MTT比色法检测不同浓度的 L eptin对人成骨细胞增殖的影响 ,同时观察不同浓度 L eptin条件下骨钙素浓度的变化 ,并用细胞总蛋白较正。结果 体外培养的人成骨细胞大多呈长梭型 ,碱性磷酸酶组织化学染色、骨钙素免疫荧光组织化学染色阳性、培养液中加入 β-甘油磷酸及 L-抗坏血酸后钙茜素红染色阳性。在加入不同浓度 L eptin后第 1、2、3天 ,人成骨细胞增殖无显著变化。L eptin可刺激骨钙素分泌 ,呈浓度依赖性 (P<0 .0 5 ) ,但无时间依赖性 (P>0 .0 5 )。结论  L eptin对人成骨细胞增殖无影响 ,但可刺激人成骨细胞功能。

 
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