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osteocalcin production
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  骨钙素分泌
     Results Alendronate had no inhitory effects on osteoblastic proliferation except at a higher concentration (1×10 -5 mol/L),but increased ALP activity and osteocalcin production at concentrations of 1×10 -7 mol/L,1×10 -5 mol/L;
     结果 阿仑膦酸钠浓度达 1× 10 -5mol/L时抑制成骨细胞增殖 ; 浓度为 1× 10 -7、1× 10 -5mol/L时增强成骨细胞的碱性膦酸酶活性和骨钙素分泌 ;
短句来源
     Methods Osteoblast-like cell line UMR106 were cultured in the medium containing 10 -7mol/L to 10 -3mol/L NaF. Cell proliferation was detected by cell growth curve and MTT colorimetry. Alkaline phosphatase(ALP)activity and osteocalcin production examined by P-nitrophenyl sodium phosphate(PNPP)colorimetry and radioimmunologic(RIA)assay respectively reflected cell differentiation.
     方法 用细胞生长曲线和四唑盐比色 (MTT)法测定细胞增殖 ,对硝基苯基磷酸二钠盐比色 (PNPP)法检测碱性磷酸酶 (ALP)活性及放免法测定骨钙素分泌量 ,研究不同浓度 ( 10 -7mol/L~ 10 -3 mol/L)氟化钠 (NaF)对成骨样细胞UMR10 6增殖和分化功能的影响。
短句来源
     Methods:Using the method of in vitro cell culcure,the influences of CCaS on the osteoblasts functions,such as cell proliferation,ALP activity,osteocalcin production,were detected.
     方法:采用体外培养成骨细胞的方法,观察材料浸提液对成骨细胞增殖、碱性磷酸酶和骨钙素分泌能力的影响。
短句来源
     Both estriol and 17-beta-estradiol had no influence on ALP activity and osteocalcin production in MG-63 cells.
     不同浓度(10~(-10)、10~(-6)、mol/L)的雌三醇或雌二醇对 MG-63细胞内 ALP 活性及骨钙素分泌量均无显著影响。
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  “osteocalcin production”译为未确定词的双语例句
     Methods Using the method of in vitro cell culture,the influence of Alendronate at concentrations of 1×10 -11 mol/L,1×10 -9 mol/L,1×10 -7 mol/L,and 1×10 -5 mol/L on the osteoblastic function (proliferation,ALP activity,osteocalcin production and bone formation) was detected.
     方法 采用体外培养成骨细胞的方法 ,观察不同浓度的阿仑膦酸钠 (1× 10 -11、1× 10 -9、1× 10 -7、1× 10 -5mol/L)对成骨细胞的增殖、碱性膦酸酶活性、分泌骨钙素及成骨能力的影响。
短句来源
     RUSULTS:Western blot analysis found that the infected MSCs expressed Osf2/Cbfa1 gene. ALP activity and osteocalcin production were markedly upregulated on day 4 in the infection group compared with the control group and the single inducement group.
     结果:感染后MSCs检测到Osf2/Cbfa1表达,从第4天开始,ATP活性和骨钙素含量较单纯诱导组和对照组显著增高。
短句来源
     Results TGF-βincreased DNA content, but inhibited ALP activi ty, osteocalcin production and mineralization of the matrix formed by osteoblast -like cells.
     结果TGF-β增加成骨样细胞DNA含量,抑制ALP活性、骨钙素的产生和矿化骨基质的形成。
短句来源
     On the contrary, stretching at 1000μεand 1500μεinhabited cells proliferation, collagen type I secretion , extracellular calcium deposition ,ALP activity and osteocalcin production.
     而1000με和1500με的应变水平抑制了成骨细胞的增殖、Ⅰ型胶原合成、胞外钙基质分泌、碱性磷酸酶活力和骨钙素合成。
短句来源
     METHODS:The rabbit MSCs were isolated and cultured in vitro,then infected with Osf2/Cbfa1 recombinant adenovirus. The expression of Osf2/Cbfa1 was detected by western blot analysis. Alkaline phosphatase(ALP) activity and expression of osteocalcin production as the osteoblast markers,were measured by the 4 nitrophenylphosphate method and radioimmunoassay,respectively.
     方法:分离培养兔MSCs,用重组Osf2/Cbfa1腺病毒感染MSCs,蛋白印迹检测目的基因表达,对硝基苯磷酸二钠法和放免法分别检测成骨细胞标志基因碱性磷酸酶(ALP)和骨钙素表达。
短句来源
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  相似匹配句对
     Results Progesterone stimulated osteocalcin gene expression and production.
     结果 孕酮刺激骨钙素基因表达及其合成。
短句来源
     Cleaner Production
     谈电镀清洁生产
     NEW PRODUCTION
     新品视窗
短句来源
     Progesterone increases osteocalcin production in vitro by regulating osteocalcin gene expression
     孕酮从基因水平调节骨钙素的合成
短句来源
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  osteocalcin production
The effect of MK-7 in increasing protein content, alkaline phosphatase activity, and osteocalcin production in the cells was completely blocked by cycloheximide.
      
Osteocalcin assay, a method to evaluate the bone formation potential, has shown that the osteocalcin production in chitosan-modified 3-D PDLLA scaffold group was significantly higher (p >amp;lt; 0.05) than that of in control.
      
ALP activity and osteocalcin production also increased with increasing porosity and time after implantation.
      
After 2 weeks of subculture, alkaline phosphatase (ALP) activity and osteocalcin production of MSCs/HA composites with Dex were higher than those without, and increased with increasing porosity.
      
The expression of some biochemical parameters of osteoblastic phenotype (alkaline phosphatase specific activity, collagen and osteocalcin production) and some indications on cells morphology obtained by scanning electron microscopy were evaluated.
      
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Objective To investigate the effect of progestin on the gene expression and production of osteocalcin in vitro in fetal rat calvarial osteoblasts. Methods Fetal rat calvarial osteoblasts were cultured in the medium with different concentrations of progesterone for nearly 20 days. Osteocalcin mRNA and the amount of osteocalcin in the conditioned medium were determined by northern blot and RIA analysis at different periods of culture. Results Progesterone stimulated osteocalcin gene expression and production....

Objective To investigate the effect of progestin on the gene expression and production of osteocalcin in vitro in fetal rat calvarial osteoblasts. Methods Fetal rat calvarial osteoblasts were cultured in the medium with different concentrations of progesterone for nearly 20 days. Osteocalcin mRNA and the amount of osteocalcin in the conditioned medium were determined by northern blot and RIA analysis at different periods of culture. Results Progesterone stimulated osteocalcin gene expression and production. In progesterone treated cultures the maximal expression of gene was 2 times higher than the control. The effect of the hormone was time and dose dependent. The amount of osteocalcin in the conditioned medium and the osteocalcin mRNA were increased in the middle and late periods of culture. In addition, 10 -6 mol/L progesterone acted more significantely on both gene expression and production of osteocalcin than the rest three doses of hormone. Conclusion Progesterone was involved directly in osteocalcin production by regulating gene expression and this effect of progesterone was time and dose dependent.

目的 观察孕激素对离体成骨细胞骨钙素基因表达及其合成的影响。方法 胎鼠头盖骨成骨细胞在含有不同浓度孕酮的培养基中培养约20 天。培养不同时期的骨钙素m R N A 含量及骨钙素的生成量通过 Northern 印迹杂交及放免的方法测定。结果 孕酮刺激骨钙素基因表达及其合成。其对m R N A 表达的最大作用可达对照组的2 倍以上。激素的作用呈时间及剂量依赖性。骨钙素m R N A 表达及其生成的增加见于培养的中、晚期阶段,提示孕激素对成熟的成骨细胞更具潜能。此外,所选用的4 种浓度的孕酮以浓度为10 - 6 mol/ L 时作用最明显。结论 孕酮从基因水平调节离体胎鼠成骨细胞骨钙素的生成,这种调节呈现时间及剂量依赖。

Objective\ To assess the effect of sodium fluoride(NaF)on rat osteoblasts and the antagonism of zinc sulfate(ZnSO 4)on NaF.Methods\ Osteoblasts obtained from the calvariae of newborn rats were cultured in medium with different concentrations of NaF and /or ZnSO 4.Cell proliferation was detected by MTT method and differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion.Results NaF increased cell proliferation and differentiation in a double regulation manner,i.e.low...

Objective\ To assess the effect of sodium fluoride(NaF)on rat osteoblasts and the antagonism of zinc sulfate(ZnSO 4)on NaF.Methods\ Osteoblasts obtained from the calvariae of newborn rats were cultured in medium with different concentrations of NaF and /or ZnSO 4.Cell proliferation was detected by MTT method and differentiation was determined by measuring alkaline phosphatase(ALP) activity and osteocalcin secretion.Results NaF increased cell proliferation and differentiation in a double regulation manner,i.e.low doses of NaF stimulated both proliferation and ALP activity of osteoblasts,while high doses suppressed them. However,all doses of NaF we used increased osteocalcin production.ZnSO 4 was synergistic with low doses of NaF and antagonistic to high doses of NaF by restoring partly the suppressive effect of NaF on cell proliferation and ALP activity,as well decreasing the enhancement of osteocalcin secretion by NaF. Conclusion NaF acts on osteoblast proliferation in a double regulated manner and possesses multiple effects on cell differentiation.10 -5 M ZnSO 4 antagonizes high doses of NaF,but does not antagonizes low doses of NaF.

目的 观察不同剂量氟化钠 ( Na F)对成骨细胞的影响及硫酸锌 ( Zn SO4 )的拮抗作用 ,为临床治疗提供实验依据。方法 取新生 2 4 h SD鼠头盖骨分离成骨细胞 ,在不同浓度的 Na F和 /或 Zn SO4( 10 -5mol/L)中培养。经 MTT法测定细胞增殖 ,细胞分化由碱性磷酸酶 ( AL P)和骨钙素水平测定得到。结果  Na F对成骨细胞增殖及分化呈双向调节 ,主要表现为大剂量抑制 ,小剂量促进。当 Na F浓度为10 -6mol/L及 10 -5mol/L时 ,细胞增殖及碱性磷酸酶活性 ( AL P)均增加 ( P<0 .0 5)。但当 Na F浓度增加至 10 -4 mol/L及 5× 10 -4 mol/L时 ,其对细胞增殖及 ALP活性呈现抑制作用。然而 ,不同剂量的 Na F对骨钙素均表现为促进其分泌 ( P<0 .0 5)。Zn SO4 与低浓度的 Na F( 10 -6mol/L、10 -5mol/L)有协同作用 ,而对高浓度的 Na F( 10 -4 mol/L、5× 10 -4 mol/L )拮抗 ,使 Na F对细胞增殖率及 ALP活性的抑制作用减弱 ,并降低高...

目的 观察不同剂量氟化钠 ( Na F)对成骨细胞的影响及硫酸锌 ( Zn SO4 )的拮抗作用 ,为临床治疗提供实验依据。方法 取新生 2 4 h SD鼠头盖骨分离成骨细胞 ,在不同浓度的 Na F和 /或 Zn SO4( 10 -5mol/L)中培养。经 MTT法测定细胞增殖 ,细胞分化由碱性磷酸酶 ( AL P)和骨钙素水平测定得到。结果  Na F对成骨细胞增殖及分化呈双向调节 ,主要表现为大剂量抑制 ,小剂量促进。当 Na F浓度为10 -6mol/L及 10 -5mol/L时 ,细胞增殖及碱性磷酸酶活性 ( AL P)均增加 ( P<0 .0 5)。但当 Na F浓度增加至 10 -4 mol/L及 5× 10 -4 mol/L时 ,其对细胞增殖及 ALP活性呈现抑制作用。然而 ,不同剂量的 Na F对骨钙素均表现为促进其分泌 ( P<0 .0 5)。Zn SO4 与低浓度的 Na F( 10 -6mol/L、10 -5mol/L)有协同作用 ,而对高浓度的 Na F( 10 -4 mol/L、5× 10 -4 mol/L )拮抗 ,使 Na F对细胞增殖率及 ALP活性的抑制作用减弱 ,并降低高浓度 Na F所刺激的骨钙素分泌。结论  Na F对离体成骨细胞的增殖呈双向调节 ,而对细胞分化的影响呈多样性 ,早期表现为剂量依赖性双向调节 ,后期仅呈现单向刺激。 10 -5mol/L Zn SO4 拮抗大剂量 Na F,对低浓度 Na F无拮抗作用

Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence...

Objective To observe the effect of leptin on osteoblast. Methods Human osteoblast primary culture was carried out, and the morphology and function of osteoblast were observed. The effects of different levels of leptin on osteoblast in different days were assessed by MTT colorimetry. Osteocalcin production was measured also. Results Human osteoblasts were fusiform in shape and were positive for alkaline phosphatase by histochemical staining, positive for osteocalcin by immunofluorescence staining, and positive by Alizarin Reds staining after mineralized upon supplementation with ascorbate and β glycerophosphate. On the first, second and third days, the prolifertion of osteoblast, cultured with different concentrations of leptin, had no changes. The leptin stimulated synthesis of osteocalcin of cells was found to be dose dependent ( P <0.05), but not time dependent ( P >0.05). Conclusion The above data indicated that there were no evidences for the effects of leptin on the proliferation of human osteoblast, but leptin could enhance the function of human osteoblast.

目的 观察瘦素 (leptin)对人成骨细胞增殖和功能的影响。方法 培养人成骨细胞 ,并观察其形态及功能变化。采用 MTT比色法检测不同浓度的 L eptin对人成骨细胞增殖的影响 ,同时观察不同浓度 L eptin条件下骨钙素浓度的变化 ,并用细胞总蛋白较正。结果 体外培养的人成骨细胞大多呈长梭型 ,碱性磷酸酶组织化学染色、骨钙素免疫荧光组织化学染色阳性、培养液中加入 β-甘油磷酸及 L-抗坏血酸后钙茜素红染色阳性。在加入不同浓度 L eptin后第 1、2、3天 ,人成骨细胞增殖无显著变化。L eptin可刺激骨钙素分泌 ,呈浓度依赖性 (P<0 .0 5 ) ,但无时间依赖性 (P>0 .0 5 )。结论  L eptin对人成骨细胞增殖无影响 ,但可刺激人成骨细胞功能。

 
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