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osteocalcin secretion
相关语句
  骨钙素分泌
     Methods: Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10 -9 mol/L ̄10 -6 mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed.
     方法:胎鼠头盖骨成骨细胞在体外经不同浓度(10-9mol/L~10-6mol/L)的孕酮作用后,对其细胞增殖、碱性磷酸酶(ALP)活性、骨钙素mRNA表达、骨钙素分泌及骨小结形成进行检测分析。
短句来源
     At 1, 2 and 4 days, the amount of DNA synthesis, thymidine incorporation of methyl-thiazol-tetrazolium (MTT), activity of alkaline phosphatase and amount of osteocalcin secretion were detected.
     在超声波作用1,2,4d后检测各组DNA合成量、四甲基偶氮唑蓝摄取情况、碱性磷酸酶活性及骨钙素分泌量。
短句来源
     The effects of TGF-β1(0.5 μg/L,1 μg/L,2 μg/L) on alkaline phosphatase activity of PDLCs in elderly people were measured with alkaline phosphatase activity assay kit; the osteocalcin secretion of PDLCs in elderly people was determined by radioimmunoassay with osteocalcin immunoradiometric kit. The results of elderly people were compared with those of young people.
     方法:将不同浓度的TGF-β1(0.5μg/L,1μg/L,2μg/L)与体外培养的老年人牙周膜细胞一同培养后,采用酶动力学方法和放射免疫法检测碱性磷酸酶活性和骨钙素分泌水平,并与青年人牙周膜细胞进行比较。
短句来源
     METHODS:The PDLCs of elderly or young people were isolated and cultured. The effects of chitosan(0.05 g/L,0.1 g/L,0.2 g/L) on alkaline phosphatase activity of PDLCs in elderly people was measured with alkaline phosphatase activity assay kit; the osteocalcin secretion of PDLCs in elderly people were determined by radioimmunoassay with osteocalcin immunoradiometric kit,and they were compared with those of PDLCs in young people.
     方法:将不同浓度的Chi(0.05g/L,0.1g/L,0.2g/L)分别与体外培养的老年人牙周膜细胞一同培养后,采用酶动力学方法和放射免疫法检测老年人牙周膜细胞碱性磷酸酶活性和骨钙素分泌水平,并与青年人牙周膜细胞进行比较。
短句来源
     SES also significantly improved osteogenic differentiation indices including alkaline phosphatase activity, osteocalcin secretion and calcium deposition level.
     TFE不影响MSCs的成骨性分化,但SES显著提高碱性磷酸酶活性、骨钙素分泌量和钙盐沉积量。
短句来源
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  “osteocalcin secretion”译为未确定词的双语例句
     When the HPLF were treated with the combination of IL 1ra and IL 1β,the osteocalcin secretion was lower than the group of only IL 1β.
     一定浓度的IL 1ra与IL 1β共同作用HPLF后 ,骨钙素浓度比单独IL 1β作用组的低。
短句来源
     Osteocalcin secretion and the expression of type X collagen mRNA in an in vitro model of vascular calcification
     体外血管钙化模型中骨钙素的分泌和X型胶原的表达
短句来源
     Objective This study was conducted to investigate the regulated effects of IL 1ra (interleukin 1 receptor antagonist,IL 1ra) on osteocalcin secretion of HPLF(Human Periodontal Ligamental Fibroblasts).
     目的 观察白细胞介素 1受体拮抗剂 (IL 1ra)与IL 1β对人牙周膜成纤维细胞 (HPLF)骨钙素分泌的影响。
短句来源
     Results: 1,25(OH) 2D 3 could stimulate osteocalcin secretion dramatically, but TGF-β inhibited its secretion.
     结果:1,25(OH)2D3对骨钙素的合成分泌具有明显增强作用,胰岛素、EGF对其合成有促进作用,但较弱。
短句来源
     Effects of IL 1ra on the osteocalcin secretion of HPLF
     IL-1ra对人牙周膜成纤维细胞骨钙素分泌的调节作用
短句来源
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  相似匹配句对
     Effects of IL 1ra on the osteocalcin secretion of HPLF
     IL-1ra对人牙周膜成纤维细胞骨钙素分泌的调节作用
短句来源
     Result:All above concentrations of auecifemine were positive on the activity of ALP and the secretion of osteocalcin.
     结果:雌三醇对成骨细胞ALP活性和骨钙素分泌的影响均呈正相关,即各浓度雌三醇对ALP活性和骨钙素的分泌均有刺激作用,并与雌三醇剂量呈正相关。
短句来源
     ② secretion of glycosaminoglycan;
     ②糖胺多糖的分泌检测。
短句来源
     and (3) vesicle secretion.
     (3)囊泡分泌。
短句来源
     ⑦ assay of osteocalcin.
     ⑦骨钙素的测定结果。
短句来源
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  osteocalcin secretion
Supplementation of culture medium with taurine enhanced alkaline phosphatase activity and osteocalcin secretion.
      
Although mitogenic concentrations of aluminum ion potentiated the 1,25 dihydroxyvitamin D3-dependent stimulation of osteocalcin secretion, they significantly inhibited the hormone-mediated activation of cellular alkaline phosphatase activity.
      
The effects of mitogenic doses of aluminum ion on basal osteocalcin secretion by normal human osteoblasts could not be determined since there was little, if any, basal secretion of osteocalcin by these cells.
      
Thus, jaw bone cells grown on hydroxylapatite proliferated very little, while expressing discrete levels of alkaline phosphatase activity and of osteocalcin secretion into the growth medium.
      
We also unexpectedly found that visfatin downregulated osteocalcin secretion from human osteoblast-like cells.
      
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Objective: To investigate the influence of progesterone on proliferation and differentiation of osteoblast at the levels of gene expression and cell functions. Methods: Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10 -9 mol/L ̄10 -6 mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed. Results: Progesterone did not influence cell proliferation;...

Objective: To investigate the influence of progesterone on proliferation and differentiation of osteoblast at the levels of gene expression and cell functions. Methods: Fetal rat calvarial osteoblasts were cultured in vitro in the presence of (10 -9 mol/L ̄10 -6 mol/L) progesterone. Cell proliferation, alkaline phosphalase (ALP) activity, osteocalcin mRNA expression and osteocalcin secretion in the medium and bone nodule formation were analyzed. Results: Progesterone did not influence cell proliferation; Progesterone enhanced the ALP activity in rat osteoblasts; Progesterone stimulated osteocalcin mRNA expression in a dose dependent manner and increased the amount of osteocalcin in the culture medium; Progesterone increased both number and area of bone nodule formation. Conclusion: Progesterone has a multi stimulating effect on the differentiation of fetal rat calvarial osteoblast, but no effect on cell proliferation.

目的:从细胞、基因水平探讨孕酮对成骨细胞增殖及分化的影响。方法:胎鼠头盖骨成骨细胞在体外经不同浓度(10-9mol/L~10-6mol/L)的孕酮作用后,对其细胞增殖、碱性磷酸酶(ALP)活性、骨钙素mRNA表达、骨钙素分泌及骨小结形成进行检测分析。结果:(1)孕酮对成骨细胞增殖无明显促进作用;(2)孕酮增加细胞ALP活性;(3)孕酮提高骨钙素mRNA表达及骨钙素的分泌,孕酮对骨钙素基因表达的刺激作用呈剂量依赖性;(4)孕酮增加骨小结形成的数量及面积。结论:孕酮对离体胎鼠头盖骨成骨细胞的分化具有多重促进效果,但对细胞的增殖无影响。

Aim: To study the effects of 1,25(OH) 2D 3, Insulin , EGF and TGF-β on the osteocalcin secretion of human dental papilla cells in vitro. Methods:A radioimmunological method was used to determine the osteocalcin secrection by the human papilla cells. Results: 1,25(OH) 2D 3 could stimulate osteocalcin secretion dramatically, but TGF-β inhibited its secretion. Conclusions: These results suggested that these factors may act as regulators of osteocalcin secretion of human dental...

Aim: To study the effects of 1,25(OH) 2D 3, Insulin , EGF and TGF-β on the osteocalcin secretion of human dental papilla cells in vitro. Methods:A radioimmunological method was used to determine the osteocalcin secrection by the human papilla cells. Results: 1,25(OH) 2D 3 could stimulate osteocalcin secretion dramatically, but TGF-β inhibited its secretion. Conclusions: These results suggested that these factors may act as regulators of osteocalcin secretion of human dental papilla cells in vitro.

目的:探讨激素和生长因子对人牙乳头细胞骨钙素合成分泌的影响;方法:采用放射免疫的方法进行测定。结果:1,25(OH)2D3对骨钙素的合成分泌具有明显增强作用,胰岛素、EGF对其合成有促进作用,但较弱。而TGF-β抑制骨钙素的合成分泌。结论:人牙乳头细胞合成分泌骨钙素受这些因子不同程度的影响,具有与成骨细胞相似的生理功能

Objective To investigate calcification of cultured aortic medial cells in vitro and acceleration by 25 hydroxycholesterol or β glycerophosphate. Methods Aortic medial cells were obtained by explantation, and the calcification was observed by von Kossa staining. Insoluble calcium precipitation in cellular layer was determined by biochemical method , and osteocalcin in the media was analyzed with radioimmunoassay. Results Two different types of primary cells were shown from culture: one with parallel...

Objective To investigate calcification of cultured aortic medial cells in vitro and acceleration by 25 hydroxycholesterol or β glycerophosphate. Methods Aortic medial cells were obtained by explantation, and the calcification was observed by von Kossa staining. Insoluble calcium precipitation in cellular layer was determined by biochemical method , and osteocalcin in the media was analyzed with radioimmunoassay. Results Two different types of primary cells were shown from culture: one with parallel cellular growth and being negative by von Kossa staining, the other cell type formed cellular nodules with positive von Kossa staining. After 28 days of cell passages, cell growth appeared no nodule formation. However, many cellular nodules and positive von Kossa staining were observed in the passaged cells treated with 25 hydroxycholesterol or β glycerophosphate, and both insoluble calcium 〔(57 80±18 50)μg/pool, (67 50±15 30)μg/pool〕and osteocalcin 〔(0 886±0 063)μg/L, (0 895±0 061)μg/L〕in the medium were significantly increased than that of the untreated cells. Conclusions Cultured aortic medial cells could be divided into two subtypes, one with the characters of smooth muscle cells, the other with the micro vascular pericytes which could calcify the extracellular matrix. 25 hydroxycholesterol and β glycerophosphate promoted the in vitro calcification, and osteocalcin secretion was increased in the process of calcification of aortic medial, suggesting that osteocalcin might participate in the aortic calcification.

目的 研究主动脉中膜细胞体外钙化情况以及 2 5 羟基胆固醇、β 甘油磷酸盐对此过程的作用。 方法 外植块法分离培养主动脉中膜细胞 ,进行vonKossa染色以观察钙化情况 ,生化检测细胞内外不溶性钙 ,放免法测定培养基质骨钙素含量。 结果 原代细胞有两种生长表现 :一种细胞平行生长 ,不形成结节 ,vonKossa染色阴性 ;一种易形成结节 ,vonKossa染色阳性。传代细胞 (对照组 )培养 2 8d无结节形成 ,vonKossa染色阴性 ,但是 ,2 5 羟基胆固醇 (胆固醇组 )、β 甘油磷酸盐 (甘油磷酸盐组 )分别刺激有细胞结节形成 ,vonKossa染色阳性 ,不溶性钙分别为 (5 7 80± 18 5 0 ) μg/孔、(6 7 5 0± 15 30 ) μg/孔 ,培养上清骨钙素分别为 (0 886± 0 0 6 3) μg/L、(0 895± 0 0 6 1) μg/L ,与对照组比较 ,差异有显著性。 结论 体外培养的主动脉中膜细胞有两种亚型 ,一种为平滑肌细胞的表型 ,另一种与微血管周细胞相似 ,并能使其细胞外基质钙化。 2 5 羟基胆固醇及 β 甘油磷酸盐均可以促进...

目的 研究主动脉中膜细胞体外钙化情况以及 2 5 羟基胆固醇、β 甘油磷酸盐对此过程的作用。 方法 外植块法分离培养主动脉中膜细胞 ,进行vonKossa染色以观察钙化情况 ,生化检测细胞内外不溶性钙 ,放免法测定培养基质骨钙素含量。 结果 原代细胞有两种生长表现 :一种细胞平行生长 ,不形成结节 ,vonKossa染色阴性 ;一种易形成结节 ,vonKossa染色阳性。传代细胞 (对照组 )培养 2 8d无结节形成 ,vonKossa染色阴性 ,但是 ,2 5 羟基胆固醇 (胆固醇组 )、β 甘油磷酸盐 (甘油磷酸盐组 )分别刺激有细胞结节形成 ,vonKossa染色阳性 ,不溶性钙分别为 (5 7 80± 18 5 0 ) μg/孔、(6 7 5 0± 15 30 ) μg/孔 ,培养上清骨钙素分别为 (0 886± 0 0 6 3) μg/L、(0 895± 0 0 6 1) μg/L ,与对照组比较 ,差异有显著性。 结论 体外培养的主动脉中膜细胞有两种亚型 ,一种为平滑肌细胞的表型 ,另一种与微血管周细胞相似 ,并能使其细胞外基质钙化。 2 5 羟基胆固醇及 β 甘油磷酸盐均可以促进这一体外钙化过程。动脉中膜体外钙化过程中骨钙素合成增多 ,可能参与了主动脉的钙化。

 
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