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entered the analysis of results
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  进入结果分析
    RESULTS: All of 20 athletes and 10 healthy adolescents entered the analysis of results.
    结果:纳入运动员20名和正常对照青少年10名,均进入结果分析
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    RESULTS: All the 50 rats finished the experiments and entered the analysis of results.
    结果:50只大鼠均完成设计方案的实验,全部进入结果分析
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    RESULTS: All the 100 recruits entered the analysis of results.
    结果:调查对象100名均进入结果分析
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AIM: To analyze responses of monoamine neurotransmitters in different brain regions to exhaustion treadmill running with or without prior endurance training. METHODS: The experiments were done from July 2002 to July 2003 in Nanjing Institute of Physical Education and Nanjing University. 50 male Sprague-Dawley rats of 8 weeks old were dived into 5 groups randomly with 10 rats in each group: sedentary group (without training), right after acute exercise group (without training, sacrificed right after acute exhaustion...

AIM: To analyze responses of monoamine neurotransmitters in different brain regions to exhaustion treadmill running with or without prior endurance training. METHODS: The experiments were done from July 2002 to July 2003 in Nanjing Institute of Physical Education and Nanjing University. 50 male Sprague-Dawley rats of 8 weeks old were dived into 5 groups randomly with 10 rats in each group: sedentary group (without training), right after acute exercise group (without training, sacrificed right after acute exhaustion treadmill running), 24 hours after acute exercise group (without training, sacrificed 24 hours later after acute exhaustion treadmill running), right after acute exercise with training group (performance endurance training program, rats were sacrificed right after acute exhaustion treadmill running ), 24 hours after acute exercise with training group (performance endurance training program, rats were sacrificed 24 hours after acute exhaustion treadmill running). Endurance training program: treadmill running training, once each a day, each a week had a day rest. Speed from 10 m per minute at the first week to 30 m per minute at the 4th week. Time from 30 minutes per day at the first week to 40 minutes per day at the 4th week. Acute exercise were exhaustion treadmill running with speed of 28 m per minute. The exhaustion standard was the rat could not keep the speed and stagnant stay in the 1/3 back part of the treadmill even with electricity stimulation for 10 s more than 3 times. Rats were sacrificed with decollation. Striatum, midbrain and hypothalamus of the brain were separated. High-performance liquid chromatography with electrochemical detection was used to determine the levels of dopamine, norepinephrine and 5-hydroxytryptamine (5-HT). RESULTS: All the 50 rats finished the experiments and entered the analysis of results. ① Dopamine in striatum of the right after acute exercise group was significantly higher than in the the sedentary group. Dopamine in midbrain of the 24 hours after acute exercise group was significantly lower than in the right after acute exercise group. ②N orepinphrine in striatum of the 24 hours after acute exercise group and the 24 hours after acute exercise with training group both significantly higher than in the sedentary group. The concentrations of norepinephrine in the midbrain of the right after acute exercise with training group was significantly lower than in the right after acute exercise group. There were no significantly differences with norepinephrine levels in hypothalamus of each group. ③ The levels of 5-HT in striatum of the right after acute exercise group were significantly higher than in the sedentary group. There were no significantly differences with 5-HT levels in both hypothalamus and midbrain of each group.CONCLUSION: Endurance treadmill training may enhance dopamine levels with dopamine synthesis and/or re-uptake. It may also improve norepinephrine basic levels and less higher 5-hydroxytryptamine in striatum and midbrain. These changes may helpful to the stabilization of central nerves system in exercise.

目的:分析不同脑区单胺类神经递质对运动耐力训练应激的反应。方法:实验于2002-07/2003-07在南京体育学院和南京大学完成。选取8周龄雄性SD大鼠50只随机分为5组:安静组(不训练);急性运动后即刻组(不训练,处死前进行急性力竭性跑台运动);急性运动后24h组(不训练,处死前一天进行急性力竭性跑台运动);耐力训练+急性运动后即刻组(实施耐力训练方案,最后进行急性力竭性跑台运动,并在运动后即刻处死);耐力训练+急性运动后24h组(实施耐力训练方案,最后进行急性力竭性跑台运动,并在运动后的24小时处死);每组10只。耐力训练方案1次/d,6d/周。跑台速度由开始的10m/min逐渐增加到第4周30m/min,运动时间由30min/d增加到40min/d。急性力竭性跑台运动方案速度为28m/min,以动物不能坚持负荷跑速,滞跑道的后1/3处达3次以上,刺激驱赶无效(10s)为力竭标准。断头处死分离出纹状体、中脑及下丘脑,经处理后采用高效液相色谱-电化学检测法测定多巴胺、去甲肾上腺素及5-羟色胺含量。结果:50只大鼠均完成设计方案的实验,全部进入结果分析。①急性运动后即刻组纹状体多巴胺含量显著高于安静组,差异有...

目的:分析不同脑区单胺类神经递质对运动耐力训练应激的反应。方法:实验于2002-07/2003-07在南京体育学院和南京大学完成。选取8周龄雄性SD大鼠50只随机分为5组:安静组(不训练);急性运动后即刻组(不训练,处死前进行急性力竭性跑台运动);急性运动后24h组(不训练,处死前一天进行急性力竭性跑台运动);耐力训练+急性运动后即刻组(实施耐力训练方案,最后进行急性力竭性跑台运动,并在运动后即刻处死);耐力训练+急性运动后24h组(实施耐力训练方案,最后进行急性力竭性跑台运动,并在运动后的24小时处死);每组10只。耐力训练方案1次/d,6d/周。跑台速度由开始的10m/min逐渐增加到第4周30m/min,运动时间由30min/d增加到40min/d。急性力竭性跑台运动方案速度为28m/min,以动物不能坚持负荷跑速,滞跑道的后1/3处达3次以上,刺激驱赶无效(10s)为力竭标准。断头处死分离出纹状体、中脑及下丘脑,经处理后采用高效液相色谱-电化学检测法测定多巴胺、去甲肾上腺素及5-羟色胺含量。结果:50只大鼠均完成设计方案的实验,全部进入结果分析。①急性运动后即刻组纹状体多巴胺含量显著高于安静组,差异有显著性。急性运动后24h组中脑内多巴胺含量显著性低于急性运动后即刻组,差异有显著性。②急性运动后24h组和耐力训练+急性运动后24h组纹状体去甲肾上腺素含量均显著性高于安静组,差异有显著性。耐力训练+急性运动后即刻组中脑去甲肾上腺素含量显著性低于急性运动后即刻组,差异有显著性。各组间下丘脑去甲肾上腺素含量差异无显著性。③急性运动后即刻组纹状体5-羟色胺含量显著性高于安静组,差异有显著性。各组间中脑5-羟色胺含量差异均无显著性。各组间下丘脑5-羟色胺含量差异无显著性。结论:大鼠耐力训练可能使恢复期多巴胺的合成和/或重摄取能力增强,纹状体和中脑去甲肾上腺素基础值适应增高,减少运动时纹状体和中脑5-羟色胺含量的升高,维持运动时中枢机能的稳定。

AIM: To study the physical and immune function of female adolescent middle-distance race athletes in stage of fore-and-aft training and evaluate the adjusted effect of nanometer-particulate ligu yuan capsule, a traditional Chinese herb. METHODS: In June 2004, 20 female adolescent middle-distance race athletes were selected from a athletic school of Beijing and divided into two groups: control group and medication group with 10 athletes in each group. Ten healthy female adolescents were selected as normal control...

AIM: To study the physical and immune function of female adolescent middle-distance race athletes in stage of fore-and-aft training and evaluate the adjusted effect of nanometer-particulate ligu yuan capsule, a traditional Chinese herb. METHODS: In June 2004, 20 female adolescent middle-distance race athletes were selected from a athletic school of Beijing and divided into two groups: control group and medication group with 10 athletes in each group. Ten healthy female adolescents were selected as normal control group. Athletes in medication group were treated with nanometer-particulate ligu yuan capsule with 3 times per-day and 2 capsules per-time. Athletes in the two groups were trained together with the same training plan: training period was from June 22, 2004 to July 28, 2004, total 5 weeks. They run 20 km every morning totally 700 km during 5 weeks, tempo run 10 km in the afternoon 3 times per-week totally 150 km during 5 weeks, random run 40 min in the afternoon 2 times per-week totally 400 minutes during 5 weeks, and the other two afternoon they could rest. All of 20 athletes were phlebotomized the first time at June 21, 2004 after a good rest period and phlebotomized the second time at July 29, 2004 after 5 weeks' training. In order to evaluate the effect which caused by exercise hyperirritability, 12 different serum cytokines (interleukine-1α, interleukine-1β, interleukine-2, interleukine-4, interleukine-6, interleukine-8, interleukine-10, vascular endothelial growth factor, interferon γ, epidermal growth factor, monocyte chemoattractant protein-1, tumor necrosis factor α) and 33 different biochemical tests (triglyceride, cholesterol, apolipoprotein A1, apolipoprotein B, high density lipoprotein cholesterol, low density lipoprotein cholesterol, alanine aminotransferase, aspartate aminotransferase, total protein, albumin, total bilirubin, direct bilirubin, total bile acid, sodium, potassium, chloride, carbon dioxide, calcium, phosphorus, magnesium, iron, Unsaturated Iron Binding Capacity, Total Iron Binding Capacity, glucose, urea, creatinine, uric acid, creatine kinase, lactate dehydrogenase, alkaline phosphatase, γ-glutamyltransferase, amylase, creatine kinase isoenzyme MB) had been detected with EVIDENCE 180 fully automatic chemiluminescence biochips analyzer and HITACHI 7600 fully automatic analyzer. RESULTS: All of 20 athletes and 10 healthy adolescents entered the analysis of results. ① The results of serum cytokines: After a good rest period, compared with the normals, the serum epidermal growth factor (EGF) of female adolescent middle-distance race athletes decreased obviously (18.94±14.04) ng/L, (62.97±38.07 ng/L). Compared before and after training, the serum interleukine-8 (IL-8) of female adolescent middle-distance race athletes decreased obviously (21.82±11.52) ng/L, < 9.7 ng/L, vascular endothelial growth factor (VEGF) also decreased obviously (113.13±47.34) ng/L, (96.90±47.30) ng/L, EGF increased obviously (18.94±14.04) ng/L, (58.93±25.48) ng/L. After had the same training period, compared with athletes in medication group, the serum interleukine-1β (IL-1β) of control group decreased obviously control group: (2.97±0.82) ng/L; medication group: (3.90±0.53) ng/L and nearly approached to the level of normal control group. ② The results of biochemical tests: Compared before and after training, the triglyceride (TG) of female adolescent middle-distance race athletes decreased obviously (0.74±0.21) mmol/L, (0.51±0.10) mmol/L, cholesterol (CH) decreased obviously (4.29±0.83) mmol/L, (3.96±0.76) mmol/L, high density lipoprotein cholesterol (HDL-C) decreased obviously (1.60±0.24) mmol/L, (1.50±0.28) mmol/L, albumin (ALB) decreased obviously (45.77±1.57) g/L, (44.23±1.88) g/L, glucose (GLU) decreased obviously (5.39±0.20) mmol/L, (4.85±0.30) mmol/L, uric acid (Ua) increased obviously (232.34 ±32.23) μmol/L, (278.89±22.69) μmol/L, these maybe related to consume the energy of body, and sodium (Na), potassium (K), chloride (Cl), decreased obviously maybe caused by physiological responses such as sweat. But compared with medication group, after dosed nanometer-particulate ligu yuan capsule, there was only TG increased obviously (0.89±0.41) mmol/L, (0.51±0.10) mmol/L in the control group, the other results of biochemical tests had no statistical difference.CONCLUSION: Heavy intensity training can cause changes of irritability, decrease of immune-ability and consumption of stored energy. Nanometer-particulate ligu yuan capsule can improve anti-stress ability, immune capability and anti-fatigue ability of body.

目的:观察青少年女性中长跑运动员这一特殊群体在大运动量集训期前后血清细胞因子及生化指标的变化,探讨运动应激条件下运动员机体变化和细胞因子免疫网络的应答机制以及纯中药制剂--纳米力谷源胶囊对其的调节作用。方法:于2004-06选择北京市某体校青少年女性中长跑运动员20名,为运动员组。又分为运动员对照组和运动员服药组,每组10名。取自无免疫性疾病、无现症、体检健康的青少年女性10名,为正常对照组。运动员服药组服用纳米力谷源胶囊(北京长城制药厂生产,批号:20040201,所含成分为葛根素、精氨酸和玉米抗氧化活性物质),3次/d,2粒/次。两组运动员共同参加训练,使用相同的训练方案:训练时间2004-06-22/07-28,共5周。每日上午:20km×7次/周×5周=700km;下午:节奏跑10km×3次/周×5周=150km,随意跑40min×2次/周×5周=400min,其余两天下午休息。运动员组休整后于2004-06-21取第1次血,第2天开始5周集训,并于集训后2004-07-29取第2次血。应用EVIDENCE180全自动化学发光生物芯片分析仪和HITACHI7600型全自动生化分析仪对20名青少年女性中...

目的:观察青少年女性中长跑运动员这一特殊群体在大运动量集训期前后血清细胞因子及生化指标的变化,探讨运动应激条件下运动员机体变化和细胞因子免疫网络的应答机制以及纯中药制剂--纳米力谷源胶囊对其的调节作用。方法:于2004-06选择北京市某体校青少年女性中长跑运动员20名,为运动员组。又分为运动员对照组和运动员服药组,每组10名。取自无免疫性疾病、无现症、体检健康的青少年女性10名,为正常对照组。运动员服药组服用纳米力谷源胶囊(北京长城制药厂生产,批号:20040201,所含成分为葛根素、精氨酸和玉米抗氧化活性物质),3次/d,2粒/次。两组运动员共同参加训练,使用相同的训练方案:训练时间2004-06-22/07-28,共5周。每日上午:20km×7次/周×5周=700km;下午:节奏跑10km×3次/周×5周=150km,随意跑40min×2次/周×5周=400min,其余两天下午休息。运动员组休整后于2004-06-21取第1次血,第2天开始5周集训,并于集训后2004-07-29取第2次血。应用EVIDENCE180全自动化学发光生物芯片分析仪和HITACHI7600型全自动生化分析仪对20名青少年女性中长跑运动员集训前后及服药后血液标本分别进行12项细胞因子(白细胞介素1α、白细胞介素1β、白细胞介素2、白细胞介素4、白细胞介素6、白细胞介素8、白细胞介素10、血管内皮生长因子、γ-干扰素、表皮生长因子、单核细胞趋化性肽1、肿瘤坏死因子α)和33项生化指标(三酰甘油、胆固醇、载脂蛋白A1、载脂蛋白B、高密度脂蛋白C、低密度脂蛋白C、谷丙转氨酶、谷草转氨酶、总蛋白、血清白蛋白、总胆红素、直接胆红素、总胆汁酸、钠、钾、氯、二氧化碳、钙、磷、镁、铁、不饱和铁结合力、总铁结合力、葡萄糖、尿素氮、肌酐、尿酸、肌酸激酶、乳酸脱氢酶、碱性磷酸酶、γ-谷氨酰转肽酶、淀粉酶、肌酸激酶同工酶)的定量检测,比较运动应激对机体的影响。结果:纳入运动员20名和正常对照青少年10名,均进入结果分析。①血清细胞因子检测结果:女性青少年长跑运动员在休整后安静状态下与正常人相比较表皮生长因子显著偏低[运动前、正常对照分别为(18.94±14.04),(62.97±38.07)ng/L];而运动员在一个时期的强度训练以后与运动前相比白细胞介素8显著下降[运动前后分别为(21.82±11.52)ng/L,<9.7ng/L],血管内皮生长因子也显著下降[运动前后分别为(113.13±47.34),(96.90±47.30)ng/L],表皮生长因子显著上升[运动前后分别为(18.94±14.04),(58.93±25.48)ng/L];运动员服药组在经过相同的训练期后与运动员对照组相比白细胞介素1β显著降低[运动员服药组、运动员对照组分别为(2.97±0.82),(3.90±0.53)ng/L],且接近正常人水平。②血清生化指标检测结果:女性青少年长跑运动员在一个时期的强度训练以后与运动前相比三酰甘油显著下降[运动前后分别为(0.74±0.21),(0.51±0.10)mmol/L]、胆固醇显著下降[运动前后分别为(4.29±0.83),(3.96±0.76)mmol/L]、高密度脂蛋白显著下降[运动前后分别为(1.60±0.24),(1.50±0.28)mmol/L]、血清白蛋白显著下降[运动前后分别为(45.77±1.57),(44.23±1.88)g/L]、葡萄糖显著下降[运动前后分别为(5.39±0.20),(4.85±0.30)mmol/L],尿酸显著上升[运动前后分别为(232.34±32.23),(278.89±22.69)μmol/L],可能与大运动量训练后造成机体贮存消耗有关,而钠、钾、氯显著下降,可能与出汗等生理反应有关。而运动员服药组服药后除三酰甘油显著上升[服药后、运动后分别为(0.89±0.41),(0.51±0.10)mmol/L]外,其他各项生化指标与运动员对照组相比变化不是很明显。结论:大运动量的集训可导致运动员机体应激性改变,免疫力下降,还可造成机体内贮能物质的大量消耗。纳米力谷源胶囊可有效的增强运动员机体的抗应激能力,提高运动员的免疫力和抗疲劳能力。

BACKGROUND: The incidence rate of spondylolysis of lumbar vertebral arch in recruits is higher during the training, and it affects the normal training. OBJECTIVE: To analyze the correlation of the incidence rate of spondylolysis of lumbar vertebral arch with training and the original lesions at waist in recruits. DESIGN: A randomized sampling survey. SETTING: Department of Orthopaedics, the 97 Hospital of Chinese PLA.PARTICIPANTS: Totally 100 male recruits aged 18.7 years averagely enrolled in 2003 were involved...

BACKGROUND: The incidence rate of spondylolysis of lumbar vertebral arch in recruits is higher during the training, and it affects the normal training. OBJECTIVE: To analyze the correlation of the incidence rate of spondylolysis of lumbar vertebral arch with training and the original lesions at waist in recruits. DESIGN: A randomized sampling survey. SETTING: Department of Orthopaedics, the 97 Hospital of Chinese PLA.PARTICIPANTS: Totally 100 male recruits aged 18.7 years averagely enrolled in 2003 were involved in the survey. METHODS: The 100 recruits were followed up for the whole course, the X-ray examination at lumbar vertebrae of right, lateral and two-slope positions were observed before training and after 100-day reinforced training.MAIN OUTCOME MEASURES: The incidence rates of spondylolysis of vertebral arch, and whether there was slippage and other aggravations in those with spondylolysis before were mainly observed. RESULTS: All the 100 recruits entered the analysis of results. ① The incidence rate of spondylolysis of vertebral arch before and after training: Before training, 5 cases had spondylolysis of L5 vertebral arch, and the incidence rate was 5%. At the end of the 100-day training, 8 cases had spondylolysis of lumbar vertebral arch, and the incidence rate increased to 8%. ② The results of X-ray examination before and after training: The X-ray examination showed that isthmus narrowing of left and L5 vertebral arch occurred in 2 cases and 1 case respectively, and spondylolysis of bilateral L5 vertebral arches occurred in 5 cases, including 1 case of degree I slippage before training. At the end of the 100-day training, the degree I slippage and below occurred in 3 of the 5 cases with spondylolysis of bilateral L5 vertebral arches before, another 3 cases with original narrowing of lumbar vertebral arch all had breakage. CONCLUSION: The incidence rate of spondylolysis of vertebral arch is associated with the training intensity. It suggestes that the examination of spinal cord should be reinforced in enrolled recruits, and the items of X-ray examination at lumbar vertebrae of right, lateral and two-slope positions should be added.

背景:新战士在新兵训练期间出现腰椎椎弓峡部断裂发病率较高并影响了正常训练。目的:分析部队新兵中腰椎椎弓峡部断裂的发病率与训练及其腰部原有病变的关系。设计:随机抽样调查。单位:解放军第九十七医院骨科。对象:某部2003年入伍的新兵100名,男性,平均年龄18.7岁。方法:对100名新兵全程追踪随访,于训练前及100d强化训练后分别摄腰部X射线正、侧位和双斜位片进行对照观察。主要观察指标:椎弓峡部断裂病例发病率,原有断裂者有无滑脱等加重现象。结果:调查对象100名均进入结果分析。①新兵训练前、后椎弓峡部断裂发病率:训练开始前的入伍新兵,L5椎弓峡部断裂5例,发病率为5%。100d训练结束时,腰椎椎弓峡部断裂8例,发病率增加至8%。②新兵训练前、后X射线检查结果:100名新战士X射线片训练开始前,左侧L5椎弓峡部变细2例,右侧L5椎弓峡部变细1例;双侧L5椎弓峡部断裂5例,其中1例呈I度滑脱。100d训练结束时,原双侧L5椎弓峡部断裂5例中I度以内滑脱3例,另3例原腰椎椎弓变细者均发生断裂。结论:腰椎椎弓峡部断裂发病率与训练强度有关。建议加强入伍新兵脊柱体检,常规加拍腰椎正、侧位和双斜位X射线片检查项目。

 
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