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entered into the result analysis
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  进入结果分析
    RESULTS All 138 rats entered into the result analysis.
    结果:138只大鼠全部进入结果分析
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    RESULTS: All 90 rats and 140 mice involved entered into the result analysis.
    结果:参加实验的90只大鼠和140只小鼠均进入结果分析
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    RESULTS: Totally 60 rats involved all entered into the result analysis.
    结果:实验纳入大鼠60只,全部进入结果分析
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    RESULTS: Totally 80 rats were entered into the result analysis.
    结果:80只大鼠全部进入结果分析
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    RESULTS: Totally 87 rats entered into the result analysis.
    结果:87只大鼠进入结果分析
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  “entered into the result analysis”译为未确定词的双语例句
    Totally 70 rats entered into the result analysis,including 12 in normal control group,9 in model group,9 in PET group,10 in EtOAc group,10 in n-BuOH group,10 in water extract group and 10 in DFLGN group.
    正常组12只,模型组9只,石油醚组9只,乙酸乙酯组10只,正丁醇组10只,水提取物组10只,利肝宁胶囊组10只。
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BACKGROUND The injury of blood-brain barrier following cerebral ischemia reperfusion is a considerate pathological basis for injury caused by cerebral ischemia and reperfusion.OBJECTIVE To investigate the effects of four most basic Chinese medicinal herbs or safflower peach seed ligusticum and red peony with actions of activating blood and resolving the stasis on the contents of nitric oxide immunoglobulins C-reactive protein CRP and complements immunological indices of serum and cerebral homogenate...

BACKGROUND The injury of blood-brain barrier following cerebral ischemia reperfusion is a considerate pathological basis for injury caused by cerebral ischemia and reperfusion.OBJECTIVE To investigate the effects of four most basic Chinese medicinal herbs or safflower peach seed ligusticum and red peony with actions of activating blood and resolving the stasis on the contents of nitric oxide immunoglobulins C-reactive protein CRP and complements immunological indices of serum and cerebral homogenate as well as the morphological and structural changes of cerebral tissue cells in rats with ischemia and reperfusion to vertify relationship between time effectiveness and quantitative effectiveness.DESIGN A randomized controlled study and evaluation by single blind. MATERIALS The experiment was completed in the Laboratory of Traumatology Institute of Guangzhou Red Cross Hospital from January 2001 to December 2002. Safflower peach seed szechwan lovge rhizome and red peony are concentrated granules of single decocting pieces the blood activating and stasis resolving decoction was prepared at 2.5 g/L according to 1∶1∶1∶2 ratio. Totally 138 adult female SD rats were selected for the experiment weighing 280-300 g provided by Animal Center of Guangzhou University of TCM.INTERVENTIONS The models rats with middle cerebral artery occlusion were set up by thread ligation 24 hours reperfusion after 2 hours middle cerebral artery occlusion. All 138 rats were randomly divided into 6 groups with 23 in each group. Sham operation group The vessels were ligated but the middle cerebral artery was not occluded. No. 1 medicated group The BASR was by gavage given in a dose of 2 g/kg 30 minutes before operation. No. 2 medicated group The BASR was by gavage given in a dose of 2.5 g/kg 30 minutes before operation. No. 3 medicated group The BASR was by gavage given in a dose of 2 g/kg for consecutive 7 days before operation. No. 4 medicated group The BASR was by gavage given in a dose of 2.5 g/kg for consecutive 7 days before operation. Control group the same volume of saline was by gavage given for consecutive 7 days before operation. ① Scoring of dysneuria 5-score system 0-1 score meant mild dysneuria 2-4 scores meant severe dysneuria for all rats were performed after consciousness following 2 hours ischemia and 24 hours reperfusion. ② After 24 hours reperfusion 10 rats in each group were at random selected for assay of levels of CRP complement 3 C3 and complement 4 C4 rate nephelometry and concentration of nitric oxide nitrate reductase method in both cerebral homogenate and serum. ③ After 24 hours reperfusion 10 rats in each group were at random selected and after anesthesia was completed the brain was quickly collected through decapitation put into a 110 ℃ drying oven till its constant weight then the water content in brain was calculated. ④ The cerebral cytomorphology in every group was observed under light microscope. ⑤ After reperfusion 3 rats in each group were randomly selected for preparation of coronal section of cerebral tissue the cerebral ultrastructure in each group was observed under transmission electronic microscope.MAIN OUTCOME MEASURES ① The results of dysneuria scoring in each group. ② The levels of CRP C3 and C4 and concentration of nitric oxide in both cerebral homogenate and serum. ③ The water content in brain. ④ The cerebral cytomorphology and the cerebral ultrastructure.RESULTS All 138 rats entered into the result analysis. ① Comparison of the extents of dysneuria of rats in each group The ratio of severe dysneuria after 24 hours ischemic reperfusion in all medicated groups was obviously lower than that in control group P < 0.01 and the ratio in No.4 medicated group was lower than that in No.2 medicated group P < 0.05. ② Comparison of water contents in brain of rats in each group The water contents in sham operation group and all medicated groups were obviously lower than that in control group P < 0.05. ③ Comparison of the nitric oxide concentration in cerebral homogenate of rats in each group The concentration in sham operation group and all medicated groups were obviously lower than that in control group P < 0.01. The concentration in No. 3 medicated group was obviously lower than that in No. 1 medicated group P < 0.05. The concentration in No. 4 medicated group was obviously lower than that in No. 2 medicated group P < 0.01. ④ Comparison of the nitric oxide concentration in serum of rats in each group The concentrations in sham operation group and all medicated groups were obviously lower than that in control group P < 0.01. The concentration in No. 3 medicated group was higher than that in No. 1 medicated group P < 0.05. The concentration in No. 4 medicated group was higher than those in No. 2 and in No. 3 medicated groups P < 0.05. ⑤ Comparison of the levels of CRP in cerebral homogenate and serum of rats in each group The levels in sham operation group and all medicated groups were lower than that in control group P < 0.05-0.01. The level in No. 3 medicated group was lower than that in No. 1 medicated group P < 0.01. The level in No. 4 medicated group was lower than those in No. 2 and in No. 3 medicated groups P <0.05-0.01. ⑥ Comparison of the levels of C3 in cerebral homogenate and serum of rats in each group The levels in sham operation group and all medicated groups were lower than that in control group P < 0.05-0.01. The level in No. 3 medicated group was lower than that in No. 1 medicated group P < 0.05. The level in No. 4 medicated group was lower than those in No. 2 and in No. 3 medicated groups P < 0.01. ⑦ Comparison of the levels of C 4 in cerebral homogenate and serum of rats in each group The levels in sham operation group and all medicated groups were lower than that in control group P < 0.05-0.01. The level in No. 3 medicated group was lower than that in No. 1 medicated group P < 0.05. The level in No. 4 medicated group was lower than those in No. 2 and in No. 3 medicated groups P < 0.01. ⑧ Comparison of the condition of cerebral edema of rats in each group In control group there was obvious cerebral congestive edema indicating an obvious infection while in medicated groups the extent of cerebral edema was milder than that in control group. ⑨ Changes of cerebral ultrastructure of rats in each group The ultrastructure in sham group was normal. In control group there obvious edema of cells capillaries and sheaths in the marginal zone of cortex necrosis and reduction of organelles of neuron. as well. In No. 3 and No. 4 medicated groups the limits of cell membranes were clear the structure was integral the chondriosomes were rich and even in size the medullated fibers were morphologically normal. And in No. 1 and No. 2 medicated groups the changes were between the twoCONCLUSION ① The scoring of dysneuria in rats was decreased after the blood-activating and stasis-resolving medicine was given and it was lower in rats that were given a longer period of medication indicating that the improved extent for dysneuria is related to prolonged medication. ② The nitric oxide concentration of cerebral tissue in rats that received the blood-activating and stasis-resolving medicine was decreased and the nitric oxide concentration of serum in the rats was increased indicating that the blood-activating and stasis-resolving medicine can reverse the anomalies of nitric concentration in different tissues after ischemic reperfusion so as to reduce cerebral injury. ③ The levels of C3 and C4 of cerebral tissue and serum in rats that received the blood-activating and stasis-resolving medicine were obviously decreased indicating that the medicine may reduce the cerebral injury through triggering complement system and the CRP was also get decreased further suggesting that the medicine can inhibiting infective reaction. ④ The longer the period of medication the milder the cerebral injury and the dose of 2.5 g/L was better in effect.

背景:脑缺血后再灌注性血脑屏障损伤是脑缺血和再灌注损伤的重要病理生理基础。目的:观察以活血化瘀的4个最基本中药(红花、桃仁、川芎、赤芍)作为施加因素对脑缺血再灌注大鼠血清和脑组织匀浆中的一氧化氮、免疫球蛋白、C 反应蛋白、补体等免疫指标及脑组织细胞形态和超微结构产生的变化,并验证其时效和量效关系。设计:随机对照实验,单盲法评估。材料:实验于2001-01/2002-12在广州市红十字会医院创伤研究所实验室完成。红花、川芎、桃仁、赤芍为单味中药饮片浓缩颗粒,按1∶1∶1∶2比例制成含2.5g/m L 生药汤剂(活血化瘀药)。实验动物选择成年雌性SD 大鼠138只,体质量280~300g,由广州中医药大学实验动物中心提供。干预:采用线栓法制备大鼠大脑中动脉阻塞模型(经2h 大脑中动脉阻断后再灌注24h)。138只大鼠随机分为6组,每组23只。假手术组:结扎各血管,不阻塞大脑中动脉。灌药1组:术前30m in 灌胃给予2g/kg活血化瘀药。灌药2组:术前30m in 灌胃给予2.5g/kg 活血化瘀药。灌药3组:术前连续7d 灌胃给予2g/kg 活血化瘀药。灌药4组:术前连续7d 灌胃给予2.5g/kg 活血化瘀药...

背景:脑缺血后再灌注性血脑屏障损伤是脑缺血和再灌注损伤的重要病理生理基础。目的:观察以活血化瘀的4个最基本中药(红花、桃仁、川芎、赤芍)作为施加因素对脑缺血再灌注大鼠血清和脑组织匀浆中的一氧化氮、免疫球蛋白、C 反应蛋白、补体等免疫指标及脑组织细胞形态和超微结构产生的变化,并验证其时效和量效关系。设计:随机对照实验,单盲法评估。材料:实验于2001-01/2002-12在广州市红十字会医院创伤研究所实验室完成。红花、川芎、桃仁、赤芍为单味中药饮片浓缩颗粒,按1∶1∶1∶2比例制成含2.5g/m L 生药汤剂(活血化瘀药)。实验动物选择成年雌性SD 大鼠138只,体质量280~300g,由广州中医药大学实验动物中心提供。干预:采用线栓法制备大鼠大脑中动脉阻塞模型(经2h 大脑中动脉阻断后再灌注24h)。138只大鼠随机分为6组,每组23只。假手术组:结扎各血管,不阻塞大脑中动脉。灌药1组:术前30m in 灌胃给予2g/kg活血化瘀药。灌药2组:术前30m in 灌胃给予2.5g/kg 活血化瘀药。灌药3组:术前连续7d 灌胃给予2g/kg 活血化瘀药。灌药4组:术前连续7d 灌胃给予2.5g/kg 活血化瘀药。对照组给予等容积生理盐水。①全部大鼠清醒后于缺血2h 再灌注24h 进行神经功能缺损评分(5分制,0~1分为神经功能轻度缺损,2~4分为神经功能重度缺损)。②再灌注24h 后从各组大鼠中随机选取10只,检测脑匀浆、血清C 反应蛋白及补体C3和C4水平采用速率散射比浊法,检测脑匀浆和血清中一氧化氮浓度采用硝酸还原酶法。③再灌注24h 后从各组大鼠中随机选取10只,麻醉后迅速断头取脑,然后在110℃烘箱中烘干至恒重,计算脑组织含水量。④光学显微镜下观察各组大鼠脑组织细胞形态。⑤再灌注24h 后从各组中随机选取3只大鼠,制备脑组织冠状切片,采用透射电镜观察各组大鼠脑组织超微结构。主要观察指标:①各组大鼠神经功能缺损评分结果。②各组大鼠脑匀浆及血清中C 反应蛋白及补体C3和C4水平,一氧化氮浓度。③各组大鼠脑组织含水量。④各组大鼠脑组织细胞形态和脑组织超微结构。结果:138只大鼠全部进入结果分析。①各组大鼠神经功能缺损程度比较:灌药各组大鼠缺血再灌注24h 后重度缺损所占比例明显低于对照组(P <0.01),灌药4组低于灌药2组(P <0.05)。②各组大鼠脑组织含水量比较:假手术组和灌药各组大鼠脑组织含水量低于对照组(P<0.05)。③各组大鼠脑匀浆一氧化氮浓度比较:假手术组和灌药各组大鼠低于对照组(P <0.01)。灌药3组低于灌药1组(P <0.05)。灌药4组低于灌药2,3组(P <0.01)。④各组大鼠血清一氧化氮浓度比较:假手术组和灌药各组血清一氧化氮浓度均高于对照组(P <0.01)。灌药3组高于灌药1组(P <0.05)。灌药4组高于灌药2,3组(P <0.05)。⑤各组大鼠脑匀浆和血清C 反应蛋白水平比较:假手术组和灌药各组低于对照组(P <0.05~0.01),灌药3组低于灌药1组(P <0.01),灌药4组低于灌药2,3组(P <0.05~0.01)。⑥各组大鼠脑匀浆和血清补体C3水平比较:假手术组和灌药各组低于对照组(P <0.05~0.01)。灌药3组低于灌药1组(P <0.05)。灌药4组低于灌药2,3组(P <0.01)。⑦各组大鼠脑匀浆和血清血清补体C4水平比较:假手术组和灌药各组低于对照组(P<0.05~0.01)。灌药3组低于灌药1组(P <0.05)。灌药4组低于灌药2,3组(P <0.01)。⑧各组大鼠脑水肿情况比较:对照组脑组织明显充血水肿,表明炎症反应明显。灌药组脑水肿、病理损害较对照组轻。⑨各组大鼠脑组织超微结构的改变:假手术组超微结构正常,对照组皮质坏死边缘区的细胞、毛细血管、髓鞘水肿明显,神经元细胞器减少,灌药3,4组细胞膜界限清楚,结构完整,线粒体丰富,大小均匀,有髓纤维形态正常,灌药1,2组介于两者之间。结论:①给予活血化瘀药大鼠神经功能缺损评分降低,用药时间长的大鼠神经功能缺损评分低,提示大鼠神经功能缺损程度改善与用药时间延长有关。②给予活血化瘀中药大鼠脑组织一氧化氮浓度降低,血清中一氧化氮浓度增高,说明活血化瘀中药可以逆转缺血再灌注后一氧化氮浓度在不同组织中的异常改变来减轻脑损伤。③给予活血化瘀中药大鼠脑组织和血清中补体C3和C4水平明显降低,说明该药可通过启动补体系统而减轻对脑组织的损伤,C 反应蛋白也明显降低,更进一步提示该药可抑制炎症反应。④用药时间越长脑损伤越轻,且用药剂量以2.5g/L 效果较好。

OBJECTIVE: Oral zhongfeng an liquid is a new dose form of traditional Chinese medicine for treating cerebrovascular diseases. Its main components are astragalus and hirudo, the former is of obvious effects of replenishing qi and activating blood, and the latter is of stronger effects of antiplatelet, antithrombosis and arteriospasm-reducing, as well as improving tissue anoxia. OBJECTIVE: To investigate the effects of oral zhongfeng an liquid on arterial thrombosis in rats, and blood coagulation and tolerance...

OBJECTIVE: Oral zhongfeng an liquid is a new dose form of traditional Chinese medicine for treating cerebrovascular diseases. Its main components are astragalus and hirudo, the former is of obvious effects of replenishing qi and activating blood, and the latter is of stronger effects of antiplatelet, antithrombosis and arteriospasm-reducing, as well as improving tissue anoxia. OBJECTIVE: To investigate the effects of oral zhongfeng an liquid on arterial thrombosis in rats, and blood coagulation and tolerance in mice. DESIGN: A completely randomized and controlled trial. SETTING: Pharmacological Division of Basic Medical College, Hebei Medical University. MATERIALS: The experiment was completed from September 2001 to April 2002 at the Pharmacological Division of Basic Medical College, Hebei Medical University. The experiment of effect of the drug on thrombosis in rats: In the first study, totally 40 Wistar rats were at random divided into five groups: zhongfeng an liquid 3.0, 6.0, 12.0 g/kg, aspirin 0.3 g/kg and control group, with 8 in each group. In the second study, totally 50 Wistar rats were also at random divided into five groups: zhongfeng an liquid 3.0 and 6.0 g/kg, naoxue kang 3.0 and 6.0 g/kg and control group, with 10 in each group. Clotting time study in mice: Totally 50 mice were randomly divided as zhongfeng an liquid 6.0, 12.0, 24.0 g/kg, aspirin 0.3 g/kg and control group, with 10 in each group. Measurement of swimming exhaustion time of mice: Totally 90 mice were randomly divided as zhongfeng an liquid 6.0 and 24.0 g/kg, naoxue kang 6.0 and 24.0 g/kg, benzedrine 0.2 g/kg and control group, with 15 in each group. METHODS: In the experiment of effect of the drug on thrombosis: For the first study, 24 hours and 1 hour before operation the rats in all groups were respectively by gavage given oral zhongfeng an liquid (3, 6, 12 g/kg), aspirin (0.3 g/kg), and water. For the second study, 24 hours and 1 hour before operation the rats were respectively by gavage given oral zhongfeng an liquid (3, 6 g/kg), naoxue kang (3, 6 g/kg) and water. After administration, ketamine 50 mg/kg was peritoneally given for anesthesia, silk ligature thrombosis was used, then the wet thrombus was weighed for comparison of difference in thrombosis among the groups. Measurement of clotting time of mice: The mice were respectively by gavage given oral zhongfeng an liquid (24.0, 12.0, 6.0 g/kg), aspirin (0.3 g/kg) and water, one hour after administration the clotting time of mice was detected with the slide method. Measurement of swimming exhaustion time of mice: The mice were respectively by gavage given oral zhongfeng an liquid (6.0, 24.0 g/kg), naoxue kang (6.0, 24.0 g/kg), Benzedrine (0.2 g/kg) and water, once a day for 5 days. On the fifth day 1 hour after administration, the swimming exhaustion time of mice was measured, the mean value of swimming exhaustion time of mice in each group was calculated. MAIN OUTCOME MEASURES: ① Inhibiting rate of thrombosis of rats in each group. ② Clotting time of mice in each group. ③ Swimming exhaustion time of mice in each group. RESULTS: All 90 rats and 140 mice involved entered into the result analysis. ① Wet quality of thrombus: In the first study, the wet quality of thrombus in rats of the oral zhongfeng an liquid 3.0, 6.0 and 12.0 g/kg groups were obviously lower than those in the naoxue kang groups of the same dose (24±4), (21±4), (16±6), (39±7) mg, (t=5.88-7.90, P < 0.01); in the second study, the wet quality of rats in the oral zhongfeng an liquid 6.0 g/kg group was obviously lower than that in the same dose naoxue kang group (23.6±2.6), (30.0±4.1), (t=4.18, P < 0.01), the wet quality of mice in the oral zhongfeng an liquid 3.0 g/kg group was obviously lower than that in the same dose naoxue kang group (30.6±2.1), (33.1±1.6) mg, (t=2.96, P < 0.05). ② Clotting time: The clotting times of the mice in the oral zhongfeng an liquid 24.0 and 12.0 g/kg groups were obviously higher than that in the control group (222±66), (190±52), (116±26) s, (t=4.02, 4.72, P < 0.01). ③ Swimming exhaustion time: The exhaustion times of mice in the oral zhongfeng an liquid 24.0 and 6.0 g/kg groups were obviously higher than that in the control group (2 512±1 244), (899±403), (502±100) s, (t=3.70-6.24, P < 0.01). CONCLUSION: Oral zhongfeng an liquid was of obvious inhibition to arterial thrombosis of rats and venous thrombosis of mice, and could enhance the tolerance of mice with a role of antifatigue

背景:中风安口服液是治疗脑血管疾病的新型中药制剂,主要成分为黄芪和水蛭,其中黄芪具有明显的益气活血作用,水蛭具有很强的抗血小板、抗血栓、缓解动脉痉挛和改善组织缺氧的作用。目的:观察中风安口服液对大鼠动脉血栓形成和对小鼠血液凝固及耐力的影响。设计:完全随机对照实验。单位:河北医科大学基础医学院药理教研室。材料:实验于2001-09/2002-04在河北医科大学药理研究室完成。药物影响血栓形成实验:第1组实验取Wistar大鼠40只,随机分为中风安3.0,6.0,12.0g/kg剂量组、阿司匹林0.3g/kg组和对照组,每组8只。第2组实验取Wistar大鼠50只,随机分为中风安3.0,6.0g/kg剂量组、脑血康3.0,6.0g/kg剂量组和对照组,每组10只。小鼠凝血时间实验:取小鼠50只,随机分为中风安6.0,12.0,24.0g/kg剂量组、阿司匹林0.3g/kg组和对照组,每组10只。小鼠游泳耗竭时间测定:取小鼠90只,随机分为中风安6.0,24.0g/kg剂量组;脑血康6.0,24.0g/kg剂量组,苯丙胺0.2g/kg组和对照组,每组15只。方法:药物影响血栓形成实验,第1组于术前24h及1h各组大...

背景:中风安口服液是治疗脑血管疾病的新型中药制剂,主要成分为黄芪和水蛭,其中黄芪具有明显的益气活血作用,水蛭具有很强的抗血小板、抗血栓、缓解动脉痉挛和改善组织缺氧的作用。目的:观察中风安口服液对大鼠动脉血栓形成和对小鼠血液凝固及耐力的影响。设计:完全随机对照实验。单位:河北医科大学基础医学院药理教研室。材料:实验于2001-09/2002-04在河北医科大学药理研究室完成。药物影响血栓形成实验:第1组实验取Wistar大鼠40只,随机分为中风安3.0,6.0,12.0g/kg剂量组、阿司匹林0.3g/kg组和对照组,每组8只。第2组实验取Wistar大鼠50只,随机分为中风安3.0,6.0g/kg剂量组、脑血康3.0,6.0g/kg剂量组和对照组,每组10只。小鼠凝血时间实验:取小鼠50只,随机分为中风安6.0,12.0,24.0g/kg剂量组、阿司匹林0.3g/kg组和对照组,每组10只。小鼠游泳耗竭时间测定:取小鼠90只,随机分为中风安6.0,24.0g/kg剂量组;脑血康6.0,24.0g/kg剂量组,苯丙胺0.2g/kg组和对照组,每组15只。方法:药物影响血栓形成实验,第1组于术前24h及1h各组大鼠分别灌胃给予中风安(3,6,12g/kg),阿司匹林(0.3g/kg)和水。第2组大鼠术前24h及1h分别灌胃给予中风安(3,6g/kg)脑血康(3,6g/kg)和水。给药后腹腔注射氯胺酮50mg/kg麻醉,采用线栓血栓法,测定各鼠血栓湿重,比较各组血栓形成的差异。小鼠凝血时间的测定,分别灌胃给予中风安(24.0,12.0,6.0g/kg),阿司匹林(0.3g/kg)和水,于灌胃后1h用玻片法观察小鼠凝血时间。小鼠游泳耗竭时间的测定,灌胃给予中风安(6.0,24.0g/kg)和脑血康(6.0,24.0g/kg)及苯丙胺(0.2g/kg,)和水。1次/d,连续灌胃5d,于第5天给药后1h测定小鼠游泳耗竭时间。计算各组小鼠游泳耗竭时间的平均值。主要观察指标:①各组大鼠血栓形成抑制率。②各组小鼠凝血时间。③各组小鼠游泳耗竭时间。结果:参加实验的90只大鼠和140只小鼠均进入结果分析。①血栓湿重:第1组实验:中风安口服液3.0,6.0,12.0g/kg剂量组明显低于对照组[(24±4),(21±4),(16±6),(39±7)mg,(t=5.88~7.90,P<0.01)];第2组实验:中风安口服液6.0g/kg剂量组明显低于相同剂量脑血康组[(23.6±2.6),(30.0±4.1),(t=4.18,P<0.01)],中风安口服液3.0g/kg剂量组低于同剂量脑血康组[(30.6±2.1),(33.1±1.6)mg,(t=2.96,P<0.05)]。②凝血时间:中风安口服液24.0,12.0g/kg剂量组明显高于对照组[(222±66),(190±52),(116±26)s,(t=4.02,4.72,P<0.01)]。③游泳耗竭时间:中风安24.0,6.0g/kg剂量组明显高于对照组[(2512±1244),(899±403),(502±100)s,(t=3.70~6.24,P<0.01)]。结论:中风安口服液对大鼠动脉血栓和小鼠静脉血栓的形成均有明显的抑制作用,并可增强小鼠的耐力,具有抗疲劳作用。

BACKGROUND: When one is in a stress state, some amino acids as neurotransmitter in his brain are of important regulating action to his cerebral functions and his psychic behaviors, and some traditional Chinese drugs can regulate the stress state of the body. OBJECTIVE: To observe the content changes of glutamic acid, aspartic acid, γ-aminobutyric acid and taurine in the hypothalamus and hippocampus of rats under repeatedly psychic stress so as to investigate the effects of tiaogan recipe, jianpi recipe, bushen...

BACKGROUND: When one is in a stress state, some amino acids as neurotransmitter in his brain are of important regulating action to his cerebral functions and his psychic behaviors, and some traditional Chinese drugs can regulate the stress state of the body. OBJECTIVE: To observe the content changes of glutamic acid, aspartic acid, γ-aminobutyric acid and taurine in the hypothalamus and hippocampus of rats under repeatedly psychic stress so as to investigate the effects of tiaogan recipe, jianpi recipe, bushen recipe and ginsenoside on them. DESIGN: A randomized grouping and controlled observation trial. SETTING:Department of Basic Theory of Traditional Chinese Medicine (TCM) of Basic Medical Science College, Guangzhou University of TCM. MATERIALS: The experiment was completed from March 2002 to January 2003 at the Animal Center of Guangzhou University of TCM. Totally 60 Wistar rats were randomly divided into 6 groups: normal, model, tiaogan, bushen, jianpi and ginsenoside groups, with 10 in each group. Compositions and doses of tiaogan recipe: radix bupleuri 5 g, fructus gardeniae 5 g, radix paeoniae alba 15 g, fructus lycii 15 g, fructus aurantii 6 g, radix rehmanniae 18 g, concha haliotidis 30 g. Compositions and doses of shenqi pill: radix rehmanniae 30 g, rhizoma dioscoreae 15 g, fructus corni 15 g, rhizoma alismatis 10 g, poria 10 g, cortex moutan radicis 10 g, ramulus cinnamomi 4 g, radix aconiti praeparata 4 g. Compositions and doses of sijunzi decoction: radix codonopsis pilosulae 20 g, rhizoma atractylodis macrocephalae 15 g, poria 15 g, radix glycyrrhizae preparata 6 g. METHODS: ① The traditional Chinese medicines were conventionally decocted and the tiaogan recipe condensed to the liquid containing 1.69 g/mL crude drug, shenqi pill containing 1.76 g/mL crude drug, sijunzi decoction containing 1.01 g/mL crude drug. Ginsenoside was prepared as 7 g/L water solution. The rats in the normal and model groups were by gavage given 2 mL of 9.0 g/L sodium chloride injection. The rats in the tiaogan, bushen, jianpi and ginsenoside groups were respectively by gavage given 2 mL of tiaogan recipe, shenqi pill, sijunzi decoction and ginsenoside solution 1 hour before immobilization stress. ② Except for rats in the normal group, those in the rest groups were all conducted for establishment of psychic stress reaction model. The rats were put into an immobilization tube, their action space was gradually reduced by using a mobile insertion piece, they were regulated to a nervous state without production of intense revolting, which was done once a day, starting with 4 hours immobilization on the first day, and later on increased by 30-60 minutes per day, for consecutive 14 days. ③ The whole brain of the rats in each group was collected by decapitation, OPA high-performance liquid chromatography was used for assays of the contents of glutamic acid, aspartic acid, γ-aminobutyric acid and taurine in hypothalamus and hippocampus of the rats. MAIN OUTCOME MEASURES: The content changes of glutamic acid, aspartic acid, γ-aminobutyric acid and taurine in the hypothalamus and hippocampus of rats in each group. RESULTS: Totally 60 rats involved all entered into the result analysis. ① The content changes of glutamic acid in the hypothalamus and hippocampus of rats in each group: Compared with normal group, the contents of glutamic acid in hypothalamus of rats in the tiaogan, jianpi, bushen and ginsenoside groups were markedly decreased (21.85±8.19), (15.76±1.80), (14.68±7.91), (21.46±5.45), (13.43±7.68) μmoL/g compared with model group, the contents in the tiaogan, bushen, jianpi and ginsenoside groups were obviously raised (11.04±3.65), (11.78±2.17), (18.67±2.98), (20.91±3.96), (17.71±1.83) μmoL/g, P < 0.05, P < 0.01, P < 0.01, P < 0.05. ② The content changes of aspartic acid in the hypothalamus and hippocampus of rats in each group: Compared with model group, the contents of aspartic acid in the hypothalamus in the jianpi and ginsenoside groups were obviously decreased (8.65±1.18), (5.72±1.32), (4.67±1.88) μmoL/g, P < 0.01, P < 0.01, while the contents in the hippocampus of rats in the jianpi, bushen and ginsenoside groups were markedly raised (2.58±0.87), (3.93±0.49), (4.52±0.98), (3.83±0.41) μmoL/g, P < 0.01, P < 0.01, P < 0.05. ③ The content changes of γ-aminobutyric acid in the hypothalamus and hippocampus of rats in each group: Compared with model group, the contents of γ-aminobutyric acid in the hypothalamus of rats in the tiaogan, jianpi, bushen and ginsenoside groups were markedly decreased (20.92±4.96), (15.87±2.90), (13.84±2.63), (14.94±3.98), (10.94±3.68) μmoL/g, P < 0.05, P < 0.05, P < 0.01, P < 0.01, while the contents in the hypothalamus were obviously raised (4.12±1.66), (4.18±1.04), (6.67±1.29), (6.11±0.99), (6.37±0.78) μmoL/g, P < 0.05, P < 0.01, P < 0.01, P < 0.01. ④ The content changes of taurine in the hypothalamus and hippocampus of rats in each group: Compared with model group, the contents of taurine in the hypothalamus of rats in the tiaogan, jianpi, bushen and ginsenoside groups were markedly decreased (10.24±1.72), (7.82±1.14), (8.00±2.05), (6.42±3.17) μmoL/g, P < 0.05, P < 0.05, P < 0.01, while the contents in the hippocampus in the jianpi and ginsenoside groups were obviously raised (12.61±3.51), (17.03±2.74), (18.04±2.14) μmoL/g, P < 0.01, P < 0.01. CONCLUSION: The central acting site of tiaogan recipe may mainly be in the hypothalamus, possibly being related with down-regulating amino acids. While the central acting sites of jianpi recipe, bushen recipe and ginsenoside may include the hippocampus and hypothalamus, being mainly related with up-regulating amino acids, through enhancing the integration of the hippocampus on stress so as to gain the effect of anti-injury of stress.

背景:人体处于应激状态时,脑内的一些氨基酸作为神经递质对脑功能和心理行为具有重要的调节作用,传统中药治疗能够调整人体的应激状态。目的:通过观察反复心理应激大鼠下丘脑和海马的谷氨酸、天冬氨酸、γ-氨基丁酸、牛磺氨酸的含量变化,探讨调肝方、健脾方、补肾方及人参总皂苷对其影响。设计:随机分组,对照观察实验。单位:广州中医药大学基础医学院中医基础理论教研室。材料:实验于2002-03/2003-01在广州中医药大学实验动物中心完成。选取Wistar大鼠60只,随机分为6组:正常组,模型组,调肝组,补肾组,健脾组,人参总皂苷组,10只/组。调肝方药组成及剂量:柴胡5g,山栀5g,白芍15g,枸杞子15g,枳壳6g,干地黄18g,石决明30g。肾气丸组成及剂量:干地黄30g,山药15g,山茱萸15g,泽泻10g,茯苓10g,丹皮10g,桂枝4g,附子4g。四君子汤组成及剂量:党参20g,白术15g,茯苓15g,炙甘草6g。方法:①常规煎煮取汁后调肝方药浓缩至含生药1.69g/mL,肾气丸浓缩至含生药1.76g/mL,四君子汤浓缩至含生药1.01g/mL,人参总皂苷配制成水溶液7g/L。正常组及模型组灌服9.0g/L氯化钠...

背景:人体处于应激状态时,脑内的一些氨基酸作为神经递质对脑功能和心理行为具有重要的调节作用,传统中药治疗能够调整人体的应激状态。目的:通过观察反复心理应激大鼠下丘脑和海马的谷氨酸、天冬氨酸、γ-氨基丁酸、牛磺氨酸的含量变化,探讨调肝方、健脾方、补肾方及人参总皂苷对其影响。设计:随机分组,对照观察实验。单位:广州中医药大学基础医学院中医基础理论教研室。材料:实验于2002-03/2003-01在广州中医药大学实验动物中心完成。选取Wistar大鼠60只,随机分为6组:正常组,模型组,调肝组,补肾组,健脾组,人参总皂苷组,10只/组。调肝方药组成及剂量:柴胡5g,山栀5g,白芍15g,枸杞子15g,枳壳6g,干地黄18g,石决明30g。肾气丸组成及剂量:干地黄30g,山药15g,山茱萸15g,泽泻10g,茯苓10g,丹皮10g,桂枝4g,附子4g。四君子汤组成及剂量:党参20g,白术15g,茯苓15g,炙甘草6g。方法:①常规煎煮取汁后调肝方药浓缩至含生药1.69g/mL,肾气丸浓缩至含生药1.76g/mL,四君子汤浓缩至含生药1.01g/mL,人参总皂苷配制成水溶液7g/L。正常组及模型组灌服9.0g/L氯化钠注射液2mL,调肝组、补肾组、健脾组及人参总皂苷组均于限制制动刺激前1h相应给予调肝方药、肾气丸煎剂、四君子汤煎剂及人参总皂苷溶液各2mL灌胃。②除正常组外,其余各组均进行心理应激反应模型的复制。将大鼠置于限制制动筒内,通过移动插片而逐步缩小大鼠的活动空间,调节到其不产生强烈反抗的紧张程度,每天限制制动1次,每天限制制动时间不同,第1天为4h,其后每次增加30~60min,连续14d。③将各组大鼠断头取全脑,采用OPA高效液相色谱分析法进行下丘脑和海马中谷氨酸、天冬氨酸、γ-氨基丁酸、谷氨酸含量的检测。主要观察指标:各组大鼠下丘脑与海马中谷氨酸、天冬氨酸、γ-氨基丁酸、牛磺氨酸含量的变化。结果:实验纳入大鼠60只,全部进入结果分析。①各组大鼠下丘脑与海马中谷氨酸含量的变化:与正常组比较,调肝组、健脾组、补肾组、人参总皂苷组下丘脑中含量均明显下降[(21.85±8.19),(15.76±1.80),(14.68±7.91),(21.46±5.45),(13.43±7.68)μmoL/g];与模型组比较,调肝组、健脾组、补肾组、人参总皂苷组海马中含量均明显升高[(11.04±3.65),(11.78±2.17),(18.67±2.98),(20.91±3.96),(17.71±1.83)μmoL/g,P<0.05,P<0.01,P<0.01,P<0.05]。②各组大鼠下丘脑与海马中天冬氨酸含量的变化:与模型组比较,健脾组和人参总皂苷组下丘脑中含量均明显下降[(8.65±1.18),(5.72±1.32),(4.67±1.88)μmoL/g,P<0.01,P<0.01],而健脾组、补肾组、人参总皂苷组海马中含量均明显升高[(2.58±0.87),(3.93±0.49),(4.52±0.98),(3.83±0.41)μmoL/g,P<0.01,P<0.01,P<0.05]。③各组大鼠下丘脑与海马中γ-氨基丁酸含量的变化:与模型组比较,调肝组、健脾组、补肾组、人参总皂苷组下丘脑中含量均明显下降[(20.92±4.96),(15.87±2.90),(13.84±2.63),(14.94±3.98),(10.94±3.68)μmoL/g,P<0.05,P<0.05,P<0.01,P<0.01],而海马中含量均明显升高[(4.12±1.66),(4.18±1.04),(6.67±1.29),(6.11±0.99),(6.37±0.78)μmoL/g,P<0.05,P<0.01,P<0.01,P<0.01]。④各组大鼠下丘脑与海马中牛磺氨酸含量的变化:与模型组比较,调肝组、健脾组、人参总皂苷组下丘脑中含量均明显下降[(10.24±1.72),(7.82±1.14),(8.00±2.05),(6.42±3.17)μmoL/g,P<0.05,P<0.05,P<0.01],而健脾组和人参总皂苷组海马中含量均明显升高[(12.61±3.51),(17.03±2.74),(18.04±2.14)μmoL/g,P<0.01,P<0.01]。结论:调肝方药的中枢作用部位可能主要在下丘脑,可能与下调氨基酸水平有关;而健脾、补肾方药及人参总皂苷的中枢作用部位可能包括海马和下丘脑,主要与上调氨基酸水平有关,通过增强海马对应激的整合作用,从而达到抗应激损伤的目的。

 
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