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entered into statistical analysis
相关语句
  进入统计分析
     RESULTS:The numbers of rats finally entered into statistical analysis were fou r groups with 12 rats each without any loss.
     结果:最终进入统计分析的大鼠保持为4组,每组12只,无缺失值。
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  相似匹配句对
     China has entered into WTO ;
     中国已加入WTO ;
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     China has be entered into the WTO.
     中国已加入WTO。
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     China has entered WTO.
     我国已加入WTO。
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     The culture entered conscious stage.
     文化进入自觉阶段。
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     56 entered the follow-up analysis.
     进入随访结果分析患者56例。
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BACKGROUND:VEGF gene promote the growth of blood vessel.Which introducing path way achieve a more ideal expression remains further discussion. OBJECTIVE:To investigate the impact of rAAV-VEGF165 gene transferred through both intravenous route and cerebrospinal fluid(CSF) route on the expression of v ascular endothelial growth factor(VEGF) in cerebral ischemic penumbra for provid ing a laboratorial gist in the VEGF gene therapy in cerebral ischemia. DESIGN:A factorial design. SETTINGS and MATERIALS:Study...

BACKGROUND:VEGF gene promote the growth of blood vessel.Which introducing path way achieve a more ideal expression remains further discussion. OBJECTIVE:To investigate the impact of rAAV-VEGF165 gene transferred through both intravenous route and cerebrospinal fluid(CSF) route on the expression of v ascular endothelial growth factor(VEGF) in cerebral ischemic penumbra for provid ing a laboratorial gist in the VEGF gene therapy in cerebral ischemia. DESIGN:A factorial design. SETTINGS and MATERIALS:Study was conducted in the animal laboratorial center o f Shenzhen People's Hospital and the Department of Pathology of Medical College of Jinan University.Forty-eight male SD rats in a SPF grade were selected. INTERVENTION:A model of permanent middle cerebral artery occlusion(MACO) was e stablished by nylon suture embolization and cerclage in SD rats. Forty-eight SD rats were randomly divided into CSF route group,intravenous route group, infarc t group and sham-operation group.The rAAV-VEGF165 gene was transferred through CSF injection or intravenous injection within 24 hours in the therapy groups.On the 7th and 14th day,the brains of the six rats from each group were removed af ter the animals were executed for immunohistochemical analysis to detect the exp ression of VEGF gene in ischemic penumbra(IP). MAIN OUTCOME MEASURES:The density of the VEGF Immunoreactive(IR)-positive cel l in the cerebral tissue over time in the rats from each group. RESULTS:The numbers of rats finally entered into statistical analysis were fou r groups with 12 rats each without any loss.The density of VEGF-IR-positive ce ll(under high power field,×400) in cerebral IP of each group at the 7th day was 74.90±2.33 in CSF group,36.27±2.61 in intravenous group,24.27±1.69) in infar ct group and 5.65±0.47 in sham-operation group respectively(P< 0.05);and at th e 14th day was 95.03±1.55 in CSF group,69.71±4.29 in intravenous group, 29.95 ±1.05 in infarct group and 7.30±0.76 in sham-operation group respectively(P< 0.05). CONCLUSION:Under the mediation of rAAV,VEGF165 gene can be transfected into th e cerebral ischemic tissue through both CSF route and intravenous route to expre ss VEGF,which can promote the formation of new vessels and protect neurocytes to treat cerebral ischemia.

背景:VEGF基因可促进脑缺血边缘区的血管生长,哪种导入途径的表达效果更理想值得探讨。目的:探讨脑池内及静脉导入rAAV-VEGF165基因对大鼠脑缺血边缘区血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)表达的影响,为VEGF基因治疗脑缺血提供实验依据。设计:析因设计。地点和对象:实验地点:深圳市人民医院动物实验中心;暨南大学医学院病理教研室。健康雄性SD大鼠48只,SPF级。干预措施:用线栓加环扎法建立SD大鼠持续性大脑中动脉闭塞(middlecerebralarteryocclusion,MCAO)模型。SD大鼠48只,随机分为脑脊液导入组、静脉导入组、梗死组和假手术组,治疗组于术后24h内将rAAV-VEGF165基因通过小脑延髓池或静脉内导入。各组分别取6只大鼠于术后7d和14d断头取脑,免疫组织化学染色检测VEGF的表达。主要观察指标:各组大鼠不同时间脑组织VEGF阳性细胞密度。结果:最终进入统计分析的大鼠保持为4组,每组12只,无缺失值。各组脑缺血边缘区VEGF阳性细胞密度(个/高倍视野,×400)分别为:7d组:脑脊液导入组74.90±2.33、...

背景:VEGF基因可促进脑缺血边缘区的血管生长,哪种导入途径的表达效果更理想值得探讨。目的:探讨脑池内及静脉导入rAAV-VEGF165基因对大鼠脑缺血边缘区血管内皮细胞生长因子(vascularendothelialgrowthfactor,VEGF)表达的影响,为VEGF基因治疗脑缺血提供实验依据。设计:析因设计。地点和对象:实验地点:深圳市人民医院动物实验中心;暨南大学医学院病理教研室。健康雄性SD大鼠48只,SPF级。干预措施:用线栓加环扎法建立SD大鼠持续性大脑中动脉闭塞(middlecerebralarteryocclusion,MCAO)模型。SD大鼠48只,随机分为脑脊液导入组、静脉导入组、梗死组和假手术组,治疗组于术后24h内将rAAV-VEGF165基因通过小脑延髓池或静脉内导入。各组分别取6只大鼠于术后7d和14d断头取脑,免疫组织化学染色检测VEGF的表达。主要观察指标:各组大鼠不同时间脑组织VEGF阳性细胞密度。结果:最终进入统计分析的大鼠保持为4组,每组12只,无缺失值。各组脑缺血边缘区VEGF阳性细胞密度(个/高倍视野,×400)分别为:7d组:脑脊液导入组74.90±2.33、静脉导入组36.27±2.61、梗死组24.27±1.69和假手术组5.65±0.47(P<0.05);14d组:脑脊液导入组95.03±1.55、静脉导入组69.17±4.29、单纯梗死组29.95±1.05和假手术组7.30±0.76(P<0.05)。结论:

 
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