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horseradish peroxidase-labelled
相关语句
  辣根过氧化物酶标记的
     The complex of Estriol and Biotin(E3-Biotin) was prepared by a simple procedure. E3-Biotin and horseradish peroxidase-labelled avidin(HRP-Avidin) were regarded as a probe in enzyme immunoassay(EIA). A sensitive EIA was developed and applied to determining E3 in serum.
     通过简单的全合成获得了共价结合的雌三醇 -生物素复合物 ( E3 -Biotin) ,结合辣根过氧化物酶标记的亲合素 ( HRP-Avidin)作为免疫分析探针 ,建立了灵敏的酶联吸附免疫分析新方法以测定血清中的雌三醇 .
短句来源
     The Riemerella antipestifer (RA)Serotype 2 treated with ultrasonic cracking was used as coated antigen, horseradish peroxidase-labelled rabbit-anti-duck IgG was taken as the second antibody, Tetramethyl Benzidine di hydrochloride (TMB) was used as coloring agent in this test.
     以2型鸭疫里氏杆菌裂解物作为包被抗原,以辣根过氧化物酶标记的兔抗鸭IgG抗体为第二抗体,以TMB为显色剂,成功地建立了检测鸭血清中2型鸭疫里氏杆菌抗体的间接EL1SA方法。
短句来源
  “horseradish peroxidase-labelled”译为未确定词的双语例句
     Horseradish peroxidase-labelled pathogenic strain DNA probes did not react with PCR products of B, C, G, T strains;
     而用非致病性虫株DNA探针与B、C、G、T的DNA斑点杂交呈阳性反应。
短句来源
     THE APPLICATION OF HORSERADISH PEROXIDASE-LABELLED ANTIBODY TO IMMUNE LOCALIZATION Ⅰ.METHOD FOR THE LOCALIZATION OF HBsAg IN LIVER TISSUE UNDER LIGHT MICROSCOPY
     辣根过氧化物酶标记抗体在免疫定位上的应用 Ⅰ.肝组织内乙型肝炎表面抗原的光学显微镜定位方法
短句来源
     THE APPLICATION OF HORSERADISH PEROXIDASE-LABELLED ANTIBODY TO IMMUNE LOCALIZATION Ⅱ.METHOD FOR LOCALIZATION OF HBsAg IN HEPATOCYTES UNDER ELECTRON MICROSCOPY
     辣根过氧化物酶标记抗体在免疫定位上的应用 Ⅱ.肝细胞内乙型肝炎表面抗原的电子显微镜定位方法
短句来源
     A FASTER DIAGNOSIS OF EPIDEMIC HEMORRHACIC FEVER WITH HORSERADISH PEROXIDASE-LABELLED STAPHYLOCOCCUS PROTEIN A
     辣根过氧化物酶标葡萄球A蛋白组化法快速诊断流行性出血热
短句来源
     Five horseradish peroxidase-labelled lectins-Con A,SBA,WGA,RCAand UEA were used to study the changes of glycocomplexes in the endometrial epitheliaand interstitial cells of sheep in dioestrus and pregnancy periods.
     用5种酶标植物凝集素(conA,SBA,WGA,UEA,RCA)作探针,研究了间情期和妊娠期绵羊子宫内膜上皮和间质糖复合物的变化。
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  相似匹配句对
     Horseradish
     辣根
短句来源
     Determination of Horseradish Peroxidase by Spectrophotometry
     3,3′,5,5′-四甲基联苯胺-H_2O_2-HRP分光光度法测定HRP的研究
短句来源
     Progress of the Mimetic Enzyme of Horseradish Peroxidase
     辣根过氧化物酶模拟酶研究进展
短句来源
     FT-Raman Study of Horseradish Peroxidase(HRP)
     辣根过氧化物酶(HRP)水溶液体系的FT-Raman光谱研究
短句来源
     Studies on horseradish peroxidase in water - organic solvents
     水-有机溶剂混合体系中辣根过氧化物酶的催化反应
短句来源
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  horseradish peroxidase-labelled
The horseradish peroxidase-labelled dendritic elements of the cuneocerebellar neurons were postsynaptic to a greater number of axon terminals which were also classified into Rd (77.5%), Pd (18.8%) and Fd (3.7%) type boutons.
      
Ultrastructurally, the profiles of horseradish peroxidase-labelled cuneocerebellar neurons could be divided into three types, namely, small, medium and large on the basis of their cross-sectional areas.
      
Ultrastructural analysis of horseradish peroxidase-labelled processes revealed that: 1.
      
The synaptic features of horseradish peroxidase-labelled recurrent collaterals in the ganglionic plexus of the cat cerebeliar co
      
Following aldehyde fixation, specimens of rat epiphyseal cartilage were examined by horseradish peroxidase-labelled lectin cytochemistry with and without prior digestion in chondroitinase ABC.
      
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A method for the localization of HBsAg in hepatocytes by means of horseradishperoxidase-labelled antibody(indirect method)at electron microscopic level isdescribed.Some problems involved are discussed.The ultrathin sections for electron microscopic examination may be preparedeither after re-embedding of a thick section used in light microscopic examination,in which HBsAg was localized immunocytochemically with horseradish peroxidase-labelled antibody,or by staining immunocytochemically with horseradish...

A method for the localization of HBsAg in hepatocytes by means of horseradishperoxidase-labelled antibody(indirect method)at electron microscopic level isdescribed.Some problems involved are discussed.The ultrathin sections for electron microscopic examination may be preparedeither after re-embedding of a thick section used in light microscopic examination,in which HBsAg was localized immunocytochemically with horseradish peroxidase-labelled antibody,or by staining immunocytochemically with horseradish peroxidase-labelled antibody directly on ultrathin section.The distribution and form of HBsAg in the HBsAg pesitive hepatocyte has beenexamined preliminarily,using horseradish peroxidase-labelled antibody under electronmicroscopy.Under electron microscopic examination there are some deposits of HBsAg withenzyme-labelled antibody,dispersed in the cytoplasma or the expanded cisternae ofthe endoplasmic reticulum of some hepatocytes which were verified light microscopicallyto be HBsAg negative.Electron microscopic examination gives more significantinformation.

本文介绍辣根过氧化物酶标记抗体对肝细胞内乙型肝炎表面抗原(HBsAg)的免疫电镜定位方法。电镜样品制备,可采用酶标免疫显色组织切片的再包埋、或直接利用超薄切片进行酶标免疫显色定位。对实验中应注意的一些问题进行了讨论。在电子显微镜下初步观察了HBsAg 阳性肝细胞中HBsAg 的分布和形态,并发现在光学显微镜下,辣根过氧化物酶标免疫定位HBsAg 完全为阴性部位的肝细胞中,有的细胞胞浆内或扩大的内质网池内,尚可见到少量散在的HBsAg 与酶标抗体的沉积物,提示电镜观察有更好的灵敏性。

The results of immunocytochemical studios on brains of mice infected with five different kinds of arbo-toga viruses: Sindbis(SIN), Eastern equine eneephalomyelitis (EEE), Western equine eneephalomyelitis (WEE), Japanese B eneephalomyelitis (JBE) ,and Tick-borne encephalitis(TBE) were reported. The method used was indirect staining of the paraffin sections with enzyme (horseradish peroxidase) -labelled antibodies. Mouse brains were innoculated with suspensions of these viruses at concentrations of 10-3-10-4....

The results of immunocytochemical studios on brains of mice infected with five different kinds of arbo-toga viruses: Sindbis(SIN), Eastern equine eneephalomyelitis (EEE), Western equine eneephalomyelitis (WEE), Japanese B eneephalomyelitis (JBE) ,and Tick-borne encephalitis(TBE) were reported. The method used was indirect staining of the paraffin sections with enzyme (horseradish peroxidase) -labelled antibodies. Mouse brains were innoculated with suspensions of these viruses at concentrations of 10-3-10-4. The infected brains were cut coronally and the paraffin sections were stained with periodated conjugated enzyme labelled antibodies,the titer of which is 1:16000 as determined by ELISA. The antigens were blocked by ten-fold dilution of normal sheep serum, before staining with primary and labelled antibodies. The results showed that specific positive reactions were seen only in the cytoplasm of brain neurons and their axons and dendrites. The reciprocal titer of antisera were found to be 32768 for SIN, 2048 for JBE and EEE, 1024 for WEE and TBE, while all parallel controls with normal rabbits serum, blocking test and sections of normal mouse brain revealed negative results. Cross reactions observed between SIN and WEE virus, may be resulted from their common antigens. The distribution of cells positive for enzyme-labelled antibodies in infected mouse brains were examined and their preferential locations were settled.

本文报告了辛比斯(SIN)、东方马脑炎(EEE)、西方马脑炎(WEE)、乙型脑炎(JBE)和森林脑炎(TBE)五种虫媒披膜病毒感染小鼠脑切片间接法酶标抗体染色。结果在神经元的胞浆及其轴突和树突内见特异阳性反应;抗血清染色滴度是SIN为32768、EEE和JBE为2048、WEE和TBE为1024;阻断试验、正常兔血清和正常鼠脑切片对照组均为阴性反应;五种病毒之间除SIN和WEE出现一定的交叉反应外其它未见到明显的交叉现象。此外还观察了酶标抗体染色阳性细胞的好发部位,这将为尸检工作提供参考依据。

A Simple and sensitive immunoenzymatic techniquc——enzyme-labelled conglutinin immunoassay (EKIA) was developed in the research. To each well of polystyrene microtitration plates coated with swin, serum albumin (SSA), 50μl of diluted rabbit anti-SSA serum, horse complement and horseradish peroxidase labelled-conglutinin (HRP-K) were added successively. The HRP-K bound to Ag-Ab-C complex was reacted with the substrate solution after incubation at 37℃ for two hours and O.D value was measured at wavelength...

A Simple and sensitive immunoenzymatic techniquc——enzyme-labelled conglutinin immunoassay (EKIA) was developed in the research. To each well of polystyrene microtitration plates coated with swin, serum albumin (SSA), 50μl of diluted rabbit anti-SSA serum, horse complement and horseradish peroxidase labelled-conglutinin (HRP-K) were added successively. The HRP-K bound to Ag-Ab-C complex was reacted with the substrate solution after incubation at 37℃ for two hours and O.D value was measured at wavelength of 492nm. The results indicated that the method was more s mple and rapid than the classical ELISA. The co-efficients of variation (C.V.) were from 7.46 to 16.67% in experiments with immunosera, showing the the satisfactory reproducibility. The purified rabbit anti-SSA IgG at 0.520 μg/ml of protein could be detected, which indicated the method was highly sensitive. It was evident that the reactions were specifically inhibited by SSA and EDTA at the final concentration of 50μg/ml and 0.03M respectively. It was showed that the method could be applied to the detection of the serum antibodies from human, swine, rabbits, quinea pigs, rats and chickens.

本文报道了一种新的酶免疫技术——酶标胶固素免疫测定(EKIA)。用猪血清白蛋白(SSA)包被微量滴定板后,加入适当稀释度的兔抗SSA血清、马血清(补体)和酶标胶固素(HRP-K)各50μl,于37℃下反应2小时。洗涤后加入底物显色,即可用分光光度计测定反应结果。本法操作简单、快速、灵敏度高,能检出兔抗SSA的IgG_(0.52)μg/ml;重复性较好,变异系数为7.46-16.67%之间;特异性强,能为相应抗原和EDTA所阻断;具有通用性,适用于人、猪、豚鼠、免、大鼠和鸡抗体的免疫检测。

 
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