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horseradish peroxidase conjugated
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  辣根过氧化物酶标记
     Methods Serum from 59 patients with anti-GBM disease were applied to inhibit monoclonal antibody against 1,3, 5(IV) NC1(Mab1,Mab3 and Mab5) and purified,horseradish peroxidase conjugated,human anti-GBM antibodies(APab-HRP) was used in competite inhibitory ELISA.
     方法用辣根过氧化物酶标记的亲和层析纯化的抗GBM自身抗体(APab-HRP)和抗α1、3、5(IV)NC1单克隆抗体(Mab1、3、5)作为识别GBM上不同抗原决定簇的探针,应用竞争性ELISA法测定抗GBM抗体的抗原决定簇。
短句来源
     β-endorphin-immunoreactive cells in the rat brain were localized using the horseradish peroxidase conjugated staphylococcal protein A(HRP-PA).
     使用辣根过氧化物酶标记葡萄球菌蛋白A(HRP—PA)对大鼠腑内β—内啡肽(β-End)免疫反应细胞进行定位。
短句来源
     Methods Serum samples from patients with PTU induced vasculitis ( n =10) and MPA ( n =10) were collected and used to inhibit monoclonal antibodies against human MPO 3D8 and 6B9 and then affinity was purified, horseradish peroxidase conjugated and human MPO antibodies determined (Pab1 HRP, Pab2 HRP) in a competitive inhibition ELISA system using soluble human MPO as solid phase ligands.
     方法 以人MPO制备的MPO亲和层析柱 ,分别从 1例PTU相关性小血管炎和 1例显微镜下型多血管炎 (MPA)患者的血浆置换液中分离纯化MPO抗体 (Pab1和Pab2 ) ,并经辣根过氧化物酶标记 (Pab1 HRP和Pab2 HRP)。
短句来源
     β-endorphin-immunoreactive neurons in the rat brain were localized by using the horseradish peroxidase conjugated staphylococcal protein A(HRP -PA), Brown orange β-endorphin-immunoreactive product in perikarya in the hypothalamic arcuate nucleus and in the pituitary were found.
     本实验使用辣根过氧化物酶标记葡萄球菌蛋白A(HRP-PA)对大鼠脑内β-内啡肽(β-EP)免疫反应细胞进行定位。
短句来源
     Soluble antigen, sonicated antigen, horseradish peroxidase conjugated IgG and SPA were used to establish ELISA separately. By these ELISA all of the differences were not significant at a level of p>0.05.
     应用嗜肺军团菌可溶性抗原、超声裂解抗原与辣根过氧化物酶标记SPA和IgG,分别建立ELISA法检测血清嗜肺军团菌抗体,其不同方法检测结果在统计学上差异不显著。
短句来源
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  辣根过氧化物酶标记的
     Methods Serum from 59 patients with anti-GBM disease were applied to inhibit monoclonal antibody against 1,3, 5(IV) NC1(Mab1,Mab3 and Mab5) and purified,horseradish peroxidase conjugated,human anti-GBM antibodies(APab-HRP) was used in competite inhibitory ELISA.
     方法用辣根过氧化物酶标记的亲和层析纯化的抗GBM自身抗体(APab-HRP)和抗α1、3、5(IV)NC1单克隆抗体(Mab1、3、5)作为识别GBM上不同抗原决定簇的探针,应用竞争性ELISA法测定抗GBM抗体的抗原决定簇。
短句来源
  “horseradish peroxidase conjugated”译为未确定词的双语例句
     The GP Ⅱb/Ⅲa contained in normal platelet extract were immobilized by the microtiter wells coated with the monoclonal antibody(SZ-21 or SZ-22). After washing, the wells were reacted with patient or control plasma, and the autoantibodies of GPⅡb/Ⅲa were detected by a horseradish peroxidase conjugated antihuman IgG.
     利用SZ-21或SZ-22(分别作用于GPⅢa和GPⅡb)包被的微孔结合正常血小板提取液中的GPⅡb/Ⅲa,洗涤后加入病人或正常对照的血浆,然后以辣根过氧化物酶联结的抗人IgG检测抗GPⅡb/Ⅲa的自身抗体。
短句来源
     coli hirudin Ⅲ was measured by triple antibodies sandwich enzyme-linked immunoadsorbent assay using antibodies from guinea pig and rabbit and a horseradish peroxidase conjugated antibody against rabbit IgG from goat.The linearities and sensitivity were 0~2()ng/ml and 0.04()ng/ml respectively.
     本测定方法的线性范围为0~2 ng/ml,灵敏度为0.04 ng/ml。
短句来源
     THE COMPARISON OF THE MEASUREMENT OF PLATELETASSOCIATED IGG WITH HORSERADISH PEROXIDASE CONJUGATED MONOCLONAL ANTIBODY AND HORSERADISH PEROXIDASE CONJUGATED POLYCONAL ANTIBODY
     酶联抗人IgG单克隆抗体与多克隆抗体测定PAIgG法的比较
短句来源
     Indirect immunoperoxidase assay (IIP) technique was used to detect specific antibodies in sera of 79 cases of acute amebiasis. The second antibody used in the test consisted of horseradish peroxidase conjugated to protein A.
     以金黄色葡萄球菌A蛋白作为第二抗体,应用间接免疫过氧化物酶试验(IIP)检测79例阿米巴病患者的血清阿米巴抗体。
短句来源
     The study was performed to analyse quantitatively the plastical changes in dendritic branching pattens of C57BL/6J mouse spinal motoneurons following prolonged training(running wheel)initiated at 4.5month of age. spinal α—motonerons were labelled with horseradish peroxidase conjugated to the β—subunit of cholera toxin(HRP—CB).
     HRP—CB标记结合sholl分析方法分析研究5、13、24月龄三个年龄组C57BL/6J小鼠脊髓前角α—运动神经元树突结构的可塑性变化,以及不同时间的长期适量运动(跑转笼)对运动神经元树突结构可塑性变化的可能作用。
短句来源
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  horseradish peroxidase conjugated
Ferritin or horseradish peroxidase conjugated goat anti-rabbit IgG was used as a secondary antibody that cross reacted with guinea pig immunoglobulins in order to reduce non-specific immunochemical reactions.
      
Following injections of horseradish peroxidase conjugated with wheat germ agglutinin into the medial nucleus accumbens of the rat, a large number of projecting pyramidal neurons in the hippocampus were retrogradely labelled.
      
Uptake of horseradish peroxidase conjugated with wheat-germ agglutinin was observed to be normal in mutant oocytes at 19°C, but was blocked at 29°C.
      
Motoneurons were labelled by intramuscular injections of horseradish peroxidase conjugated with cholera toxin subunit B.
      
Retrograde transport of horseradish peroxidase conjugated to wheat germ agglutinin following injections into parietal, occipital and temporal cortex was used.
      
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An enzyme-linked immunosorbent assay (ELISA) for the quantitation of human alpha-fetoprotein is described. This new technique provides a relatively simple, sensitive and reproducible means of the sandwich wethod. Only inexpensive equipment is used as compared with that of RIA. However, sensitivity threshold and reproducibility are within a range comparable to that of RIA. As principal reagent, peroxidase-labeled anti-human alpha-fetoprotein antibodies were prepared by periodate oxidized horseradish...

An enzyme-linked immunosorbent assay (ELISA) for the quantitation of human alpha-fetoprotein is described. This new technique provides a relatively simple, sensitive and reproducible means of the sandwich wethod. Only inexpensive equipment is used as compared with that of RIA. However, sensitivity threshold and reproducibility are within a range comparable to that of RIA. As principal reagent, peroxidase-labeled anti-human alpha-fetoprotein antibodies were prepared by periodate oxidized horseradish peroxidase conjugating to an IgG fraction of horse anti alpha-fetoprotein. purification of the conjugate was carried out on Sephadex G-200 column chromatography. A straight curve in the range 12.5-100ng/ml has been obtained. The coefficient of variation of two different concentration samples were 6.3% and 6.8% respectively. The dose-response curve covers a 100-fold range of analytic concentrations. The recovery rate of added antigen was 100.6±5.8%. Results could be obtained within 6 h. Determination results of AFP in 129 clinical samples accord with clinical diagnoses. When alpha-fetoprotein was quantitated in 20 sera both by radioimmunoassay(RIA) and enzyme-linked immunosorbent assay (ELISA), a Correlation coefficient of 0.95 was obtained between the two methods.

本文介绍了一种新的临床甲胎蛋白定量的ELIsA方法。此法操作简单、省时间、成本低廉,其灵敏度可以达到RIA水平。适合医院做为常规检查项目。辣根过氧化物酶用过碘酸钠法与抗甲胎蛋白IgG结合。酶标抗体是用SephadexG—200纯化的。标准曲线重现性良好,精密度实验批内变异系数平均为6.5%;回收率为100.6±5.8%;本文在缩短ELISA测定时间上做了改进,提出采用40℃包被和孵育,可在6小时内得到结果。本法与放射火箭电泳自显影法比较20例两法结果的相关系数r=0.95。129例血清甲胎蛋白ELISA测定,结果符合临床诊断。 (本研究标记部分工作在生物系生殖免疫研究室完成,得到刘学高副教授热情帮助和指导;临床部分工作在空军广州医院检验科完成,得到陈汉军、邸红同志大力支持和帮助;本文完成后,请第一军医大学生化室徐铃教授和白求恩大学生化室麦荫乔教授审阅。并提出宝贵意见,特此一并致谢!)

A specific sensitive and rapid ELISA (double antibody sandwich technique) with anti-human IgG and corresponding horseradish peroxidase conjugate was described for the quantitative determination of trace amounts of IgG. The chequerboard titrations were applied tor selection of optimal concentration of coating antibody and conjugate, they are 10μUg/ml and 1 : 2000 respectively. The sensitivity can reach as low as 3.0 ng/ml. It is suitable for research work as well as clinical study.Several factors...

A specific sensitive and rapid ELISA (double antibody sandwich technique) with anti-human IgG and corresponding horseradish peroxidase conjugate was described for the quantitative determination of trace amounts of IgG. The chequerboard titrations were applied tor selection of optimal concentration of coating antibody and conjugate, they are 10μUg/ml and 1 : 2000 respectively. The sensitivity can reach as low as 3.0 ng/ml. It is suitable for research work as well as clinical study.Several factors affected the method have been investigated. Reproducibility and specificity of the method were identified.

本文报道了一种特异、敏感,快速的酶联免疫吸附试验(ELISA)双抗体夹心法和抗人IgG及相应辣根过氧化物酶结合物用于测定微量IgG。用棋盘滴定法选择包被抗体和结合物的最适浓度分别为10μg/ml和1:2000,敏感性可达3.0ng/ml,适用于科研和临床研究。并探讨了影响本法的因素,确定了本法的重复性和特异性。

β-endorphin-immunoreactive neurons in the rat brain were localized by using the horseradish peroxidase conjugated staphylococcal protein A(HRP -PA), Brown orange β-endorphin-immunoreactive product in perikarya in the hypothalamic arcuate nucleus and in the pituitary were found. There were dense β-endorphin-immunoreactive granules in the ventral periaqueductal gray. The results show that HRP-PA is a valuable method for the locali-zation of β-endorphin-immunoreactive neurons in the rat brain.

本实验使用辣根过氧化物酶标记葡萄球菌蛋白A(HRP-PA)对大鼠脑内β-内啡肽(β-EP)免疫反应细胞进行定位。下丘脑弓状核和垂体的核周质中观察到棕黄色的β-EP免疫反应产物,在导水管腹侧有较密集的β-EP免疫反应颗粒。这结果表明,采用HRP-PA定位脑内β-EP免疫反应细胞乃是一种良好的方法。

 
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