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horseradish peroxidase labeled
相关语句
  辣根过氧化物酶标记的
     The distribution of lectin binding in gastrointestinal tract of human, rat and mouse was investigated using horseradish peroxidase labeled lectins-WGA, SBA, and PNA staining method.
     用三种辣根过氧化物酶标记的凝集素—WGA、SBA和PNA观察人及大小鼠的消化管各段中凝集素结合部位的分布。
短句来源
     A direct enzyme-linked immunosorbent assay(D-ELISA) is developed that uses horseradish peroxidase labeled antigen for detection of immunoglobulin M(IgM) antibodies to Toxoplasma gondii, in which polystyrene microtiter plates were sensitized with mouse anti-human μ chain monoclonal antibody to separate IgM from other classes of antibody. The presence of IgM antibodies to Toxoplasma(Tox-IgM) was then detected by sequential addition of so luble horseradish peroxidase-labeled Toxoplasma antigen and substrate.
     本文以鼠抗人μ链单克隆抗体捕获被测人血清中IgM抗体,再以辣根过氧化物酶标记的弓形虫抗原进行直接酶联免疫吸附试验,检测人血清中特异性抗弓形虫IgM抗体,并以阻断试验证实该方法的特异性。
短句来源
     After this the membrane was immerged into the chromogenic substrate to react and stop the reaction with running water. Dots were judged by visual method. Result When horseradish peroxidase labeled McAb N 15C was used to capture the cTnT of the serum from the myocardiac infarction patients, the serum titer was 1∶8;
     结果 辣根过氧化物酶标记的N1 5C单克隆抗体检测心肌梗塞患者血清 ,患者血清最高稀释为 1∶8,N1 6D检测患者血清时 ,血清稀释度可达 1∶64 ,用 2株单抗检测时正常人血清均无斑点出现。
短句来源
  “horseradish peroxidase labeled”译为未确定词的双语例句
     The distribution of Con A receptor in gastrointestinal tract of human, rat,mouse and dog was investigated using horseradish peroxidase labeled Con A staining method.
     本文采用辣根过氧化物酶标记伴刀豆球蛋白A(Con A-HRP)法来研究人和犬、大小鼠消化管各段Con A受体的分布。
短句来源
     This histochemical binding of horseradish peroxidase labeled peanut agglutinin (HRP?PNA) to paraffin sections of 18 cases of pancreatic cancer was investigated with 5 case of normal pancreas and 8 pancreatitis as the control.
     用酶标记的花生凝集素(PNA)对18例胰腺癌组织中的PNA受体进行了组织化学研究,并以正常胰腺组织(5例)和慢性胰腺炎(8例)作对照。
短句来源
     Methods The bile samples from 31 cases of cholangiocarcinomas and 13 cases of benign biliary diseases were dropped onto nitrocellulose membrane respectively,followed by lectin dot blotting with horseradish peroxidase labeled lectins,including ConA(coneanavalia ensifomis agglutinin),DSA(datura stramonium agglutinin),WGA(triticum valgaris agglutinin) and LCA(lens culinaris agglutinin).
     方法 将 31例胆管癌胆汁和 13例良性胆道疾病胆汁直接点在硝酸纤维膜 (NC)上 ,采用辣根过氧化物酶 (HRP)标记的刀豆凝集素 (ConA )、欧曼陀罗凝集素 (DSA )、麦胚凝集素 (WGA )、小扁豆凝集素 (LCA )进行点印迹分析。
短句来源
     Detection of CEA Marker of Colon-Rectal Carcinoma under Light and Electron Microscopes Using Horseradish Peroxidase Labeled Anti-CEA Fab Antibody
     用辣根过氧化物酶标记抗体Fab染色法在光镜及电镜下检测大肠癌的CEA标志
短句来源
     Three kinds of horseradish peroxidase labeled antibodies were prepared by periodate (salting-out) method, periodate (Sephadex G-200) method and two-step glutaraldehyde (salting-Out) method.
     本文制备了用NaIO_4(盐析)法、NaIO_4(G-200)和戊二醛(GA)二步法(盐析)标记的三种HRPO标记抗体。
短句来源
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  相似匹配句对
     Horseradish
     辣根
短句来源
     Determination of Horseradish Peroxidase by Spectrophotometry
     3,3′,5,5′-四甲基联苯胺-H_2O_2-HRP分光光度法测定HRP的研究
短句来源
     Progress of the Mimetic Enzyme of Horseradish Peroxidase
     辣根过氧化物酶模拟酶研究进展
短句来源
     Making and Appraisement on Daidzein Labeled with Horseradish peroxidase
     酶标记大豆甙元的制备和鉴定
短句来源
     Insulin was labeled with horseradish peroxidase by sodium periodate oxidation method.
     用高碘酸钠氧化法将辣根过氧化物酶标记于胰岛素。
短句来源
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  horseradish peroxidase labeled
The secondary horseradish peroxidase-labeled anti-mouse antibody was affinity-purified and yielded negative results when used alone.
      
The polyvinylidene difluoride sheets were incubated with the horseradish peroxidase-labeled, goat antimouse IgG as described for the first antibodies.
      
The peptides were tested for adhesion to horseradish peroxidase-labeled mucin by using the dot blot adhesion assay.
      
The membranes were rinsed three times in TBS-Tween and incubated with a horseradish peroxidase-labeled secondary antibody for 30 min at 370 C.
      
The membrane was incubated with mAb FE8, followed by horseradish peroxidase-labeled anti-mouse and chemiluminescence detection.
      
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Three kinds of horseradish peroxidase labeled antibodies were prepared by periodate (salting-out) method, periodate (Sephadex G-200) method and two-step glutaraldehyde (salting-Out) method.The result of irvimnTw.hfimip.al identification of these conjugates showed that the E/P ratio and the conjugate rate were higher in the two periodate methods than that in two-step glutaraldehyde conjugation method.The result of chessboard titration of antinuclear antibody in mouse liver frozen sections and Japanese B...

Three kinds of horseradish peroxidase labeled antibodies were prepared by periodate (salting-out) method, periodate (Sephadex G-200) method and two-step glutaraldehyde (salting-Out) method.The result of irvimnTw.hfimip.al identification of these conjugates showed that the E/P ratio and the conjugate rate were higher in the two periodate methods than that in two-step glutaraldehyde conjugation method.The result of chessboard titration of antinuclear antibody in mouse liver frozen sections and Japanese B virus in mouse brain paraffin sections also revealed that the plateau titers were higher in the periodate method than those in the two-step glutaraldehyde method.Thus the periodate method seems to be superior in this respect to the glutaraldehyde method.Five horseradish peroxidase labeled conjugates were prepared from the goat anti-rabbit IgG antibodies purified by affinity chromatography. To 10 mg of antibody, 20, 10, 5, 2.5 and 1.25 mg of horseradish peroxidase were added.The rate of increase in conjugated enzyme, the peroxidase activity and the molar E/P ratios increased with the amount of peroxidase added in biochemical determination.The result of chessboard titrations showed that the calculated molar E/P ratio coorrela-ted well with that of the plateau titer.Our result showed that higher serum plateau titers of 2560 (Antiauclear antibody) and 1280 (Japanese B encephalitis antibody) could be obtained when E/P ratio was 2.67.

本文制备了用NaIO_4(盐析)法、NaIO_4(G-200)和戊二醛(GA)二步法(盐析)标记的三种HRPO标记抗体。免疫化学鉴定结果,NaIO_4法比GA二步法酶标抗体的E/P比值高,酶的标记率也高。用抗核抗体鼠肝冷冻切片和流乙病毒感染鼠脑石蜡切片进行棋盘滴定结果,NaIO_4(盐析)法的棋盘滴度也较GA(盐析)二步法高。因此认为NaIO_4法优于GA二步法。 以不同量的酶对抗体制备了5种酶标抗体。免疫化学鉴定结果,标抗的酶量、酶活力和E/P比值随着酶加入量的增多而增高。抗核抗体和流乙抗体的棋盘滴度与E/P比值呈一致关系。E/P比值为2.67者可获得抗核抗体(2560)和流乙抗体(1280)的高棋盘滴度。

The distribution of Con A receptor in gastrointestinal tract of human, rat,mouse and dog was investigated using horseradish peroxidase labeled Con A staining method. It has been shown that Con A receptors were existed, more or less, in mucosal epithelia taken from different segments of tract. The most intense staining was noticed in supranuclear region of epithelial cells, except for gastric mucosa, which had a negative supranuclear region. The staining character of goblet cells was heterogenous: negative...

The distribution of Con A receptor in gastrointestinal tract of human, rat,mouse and dog was investigated using horseradish peroxidase labeled Con A staining method. It has been shown that Con A receptors were existed, more or less, in mucosal epithelia taken from different segments of tract. The most intense staining was noticed in supranuclear region of epithelial cells, except for gastric mucosa, which had a negative supranuclear region. The staining character of goblet cells was heterogenous: negative staining was verified in the goblet cells of small intestine from human and dog, and large intestine from rat and mouse, while the goblet cells in other place all showed more or less positive reaction. There was significant species difference of staining reaction in chief cell and parietal cell of gastric glands: both cells showed positive staining in human and dog, while only chief cells showed positive reaction in rat and mouse. Besides, Con A receptors were also found in intestinal glands, neurons of plexus of Auerbach and Meissner, smooth muscle. In a word, a highly heteroglneous cell populations were in gastrointestinal tract and possessed species difference.

本文采用辣根过氧化物酶标记伴刀豆球蛋白A(Con A-HRP)法来研究人和犬、大小鼠消化管各段Con A受体的分布。结果表明:各段消化管粘膜上皮细胞都具有不同程度的阳性反应。除胃粘膜上皮细胞的核上区为阴性外,其他各段均以核上区反应最强。杯状细胞除人和犬的小肠及大小鼠的大肠阴性反应外,均有程度不等的阳性反应。胃底腺内壁细胞和主细胞的反应有明显的种系差异,人和犬二种细胞都为阳性反应,而大小鼠只有主细胞有阳性反应。肠腺阳性反应。神经元有较强的反应。平滑肌有不同强度的反应,人、犬比大小鼠强。由此提示消化管是一个高度异质性的细胞群体,并有种系差异。

Four specimens of colon-rectal carcinomas were stained histochemically and immunochemically using horseradish peroxidase labeled anti-CEA Fab antibody for the detection of CEA tumor marker, The results proved that Fab antibody fragment labeled with horseradish peroxidase better penetrated into the tissue owing to its smaller molecular weight, In addition, this method is simple, reliable and may be used for the detection of gastrointestinal adenocarcinomas as auxiliary procedure for pathological...

Four specimens of colon-rectal carcinomas were stained histochemically and immunochemically using horseradish peroxidase labeled anti-CEA Fab antibody for the detection of CEA tumor marker, The results proved that Fab antibody fragment labeled with horseradish peroxidase better penetrated into the tissue owing to its smaller molecular weight, In addition, this method is simple, reliable and may be used for the detection of gastrointestinal adenocarcinomas as auxiliary procedure for pathological diagnosis.

采用辣根过氧化物酶标记的抗癌胚抗原(CEA)的抗体Fab片断,对4例大肠癌手术标本进行免疫组织化学染色,以检测肿瘤标志CEA。实验证明,此法简便易行、可靠,可用于检测消化系统的许多腺癌,做为病理诊断学的辅助手段。

 
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