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   human osteocalcin 的翻译结果: 查询用时:0.007秒
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human osteocalcin
相关语句
  人骨钙素
     Function of unique osteoblastic gene verified by human osteocalcin promoter in vivo
     使用人骨钙素启动子实现在体研究成骨细胞特定基因的功能
短句来源
     Methods: Parti: The knockdown effects of shRNA driven by human osteocalcin promoter without 5'UTR and hTERT core promoter with a reconstructed transcriptional start site on the expression of exogenous luciferase and endogenous P53 were evaluated.
     方法:①使用删除5'UTR的人骨钙素启动子引导shRNA表达,观察其对外源性荧光素酶基因表达的剔除作用;
短句来源
     METHODS:The experiment was conducted in the Institute of Orthopaedics of Chine se PLA and Center for Plastic Surgery of Chinese PLA,Xijing Hospital,Fourth Mili tary Medical University of Chinese PLA from November 2003 to September 2004.The human osteocalcin promoter was amplified by PCR using genomic DNA of HEK293 cell s as templates.
     方法:实验于2003-11/2004-09在解放军第四军医大学全军骨科研究所和全军整形外科中心完成。 以HEK293细胞基因组为模板,利用聚合酶链反应扩增人骨钙素启动子。
短句来源
     RESULTS:An 830 bp human osteocalcin promoter was amplified using specific prim ers.
     结果:使用特异性引物成功扩增出长度为830bp的人骨钙素启动子聚合酶链反应产物。
短句来源
     CONCLUSION:An shRNA expression vector driven by human osteocalcin promoter is successfully constructed,and the specific RNAi of bone tissue is verified,provid ing an basis for the introduction of RNAi into the field of gene therapy for ost eosarcoma.
     结论:成功使用人骨钙素启动子构建了小发卡状RNA表达载体,并实现了骨组织特异性RNA干扰,为将RNA干扰技术引入骨肉瘤基因治疗领域奠定了基础。
短句来源
  “human osteocalcin”译为未确定词的双语例句
     AIM:To construct specific shRNA expression vector driven by human osteocalcin promoter.
     目的:使用骨钙素启动子构建骨组织特异性小发卡状RNA表达载体。
短句来源
  相似匹配句对
     human;
     human ;
短句来源
     Human Serum Osteocalcin Determined by ELISA and its Clinical Application
     人血清骨钙素ELISA测定法及临床应用
短句来源
     ON HUMAN ERROR
     论人的失误
短句来源
     Function of unique osteoblastic gene verified by human osteocalcin promoter in vivo
     使用人骨钙素启动子实现在体研究成骨细胞特定基因的功能
短句来源
     ⑦ assay of osteocalcin.
     ⑦骨钙素的测定结果。
短句来源
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  human osteocalcin
We have reported that transgenic mice overexpressing human osteoblast stimulating factor-1 (osf1) under the control of the human osteocalcin promoter have a significantly higher bone mineral content and density than nontransgenic littermates.
      
This assay system could detect the N-terminal portion of osteocalcin formed during the degradation of the human osteocalcin molecule by trypsin or cathepsin D.
      
Our results indicate that this new immunoradiometric assay for the intact human osteocalcin has the potential for good discrimination between normal subjects and patients with both low and high bone turnover.
      
Clinical Validation of a New Immunoradiometric Assay for Intact Human Osteocalcin
      
This C-terminal specific radioimmunoassay detected the intact human osteocalcin in HPLC purified plasma and peritoneal dialysate from patients with terminal renal insufficiency and in extracted human bone.
      
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AIM:To construct specific shRNA expression vector driven by human osteocalcin promoter. METHODS:The experiment was conducted in the Institute of Orthopaedics of Chine se PLA and Center for Plastic Surgery of Chinese PLA,Xijing Hospital,Fourth Mili tary Medical University of Chinese PLA from November 2003 to September 2004.The human osteocalcin promoter was amplified by PCR using genomic DNA of HEK293 cell s as templates.Then,the osteocalcin promoter,shRNA coding sequence designed to knockdown...

AIM:To construct specific shRNA expression vector driven by human osteocalcin promoter. METHODS:The experiment was conducted in the Institute of Orthopaedics of Chine se PLA and Center for Plastic Surgery of Chinese PLA,Xijing Hospital,Fourth Mili tary Medical University of Chinese PLA from November 2003 to September 2004.The human osteocalcin promoter was amplified by PCR using genomic DNA of HEK293 cell s as templates.Then,the osteocalcin promoter,shRNA coding sequence designed to knockdown luciferase gene and the polyadenylation signal were cloned into the co rresponding enzyme sites of pGL3 control vector in turns.After confirmed by dou ble restriction enzyme digestion and DNA sequencing,the resulting plasmid pGL3 OCP shLuc was used to co transfect HEK293 and MG 63 cells with pGL3 control. Luciferase activities were assayed 48 hours after co transfection,and the relat ive activities were calculated. RESULTS:An 830 bp human osteocalcin promoter was amplified using specific prim ers.DNA sequencing results verified that the expression cassette of shRNA in pGL 3 OCP shLuc was consistent with that having been designed.After co transfecti on of HEK293 and MG 63 with pGL3 OCP shLuc and pGL3 control,the relative luc iferase activities of HEK293 had no difference from those of the controls,but th e relative luciferase activities of MG 63 were significantly decreased and the inhibition rate was about 69.8%. CONCLUSION:An shRNA expression vector driven by human osteocalcin promoter is successfully constructed,and the specific RNAi of bone tissue is verified,provid ing an basis for the introduction of RNAi into the field of gene therapy for ost eosarcoma.

目的:使用骨钙素启动子构建骨组织特异性小发卡状RNA表达载体。方法:实验于2003-11/2004-09在解放军第四军医大学全军骨科研究所和全军整形外科中心完成。以HEK293细胞基因组为模板,利用聚合酶链反应扩增人骨钙素启动子。将骨钙素启动子、针对虫荧光素酶的小发卡状RNA编码序列和多腺苷酸信号依次克隆至pGL3-control载体的相应酶切位点,经双酶切鉴定和DNA测序证实后命名为pGL3-OCP-shLuc。将pGL3-OCP-shLuc与pGL3-control分别共转染人胚胎肾细胞HEK293和人骨肉瘤细胞MG-63,48h后测定虫荧光素酶活性,计算相对活性。结果:使用特异性引物成功扩增出长度为830bp的人骨钙素启动子聚合酶链反应产物。DNA测序结果证实pGL3-OCP-shLuc中的小发卡状RNA表达框与设计完全一致。pGL3-OCP-shLuc与pGL3-control共转染HEK293细胞后,虫荧光素酶相对活性与对照组相比没有明显差异,而共转染MG-63细胞后虫荧光素酶相对活性显著降低,剔出效率约为69.8%。结论:成功使用人骨钙素启动子构建了小发卡状RNA表达载体,并实现了骨组织特异性RN...

目的:使用骨钙素启动子构建骨组织特异性小发卡状RNA表达载体。方法:实验于2003-11/2004-09在解放军第四军医大学全军骨科研究所和全军整形外科中心完成。以HEK293细胞基因组为模板,利用聚合酶链反应扩增人骨钙素启动子。将骨钙素启动子、针对虫荧光素酶的小发卡状RNA编码序列和多腺苷酸信号依次克隆至pGL3-control载体的相应酶切位点,经双酶切鉴定和DNA测序证实后命名为pGL3-OCP-shLuc。将pGL3-OCP-shLuc与pGL3-control分别共转染人胚胎肾细胞HEK293和人骨肉瘤细胞MG-63,48h后测定虫荧光素酶活性,计算相对活性。结果:使用特异性引物成功扩增出长度为830bp的人骨钙素启动子聚合酶链反应产物。DNA测序结果证实pGL3-OCP-shLuc中的小发卡状RNA表达框与设计完全一致。pGL3-OCP-shLuc与pGL3-control共转染HEK293细胞后,虫荧光素酶相对活性与对照组相比没有明显差异,而共转染MG-63细胞后虫荧光素酶相对活性显著降低,剔出效率约为69.8%。结论:成功使用人骨钙素启动子构建了小发卡状RNA表达载体,并实现了骨组织特异性RNA干扰,为将RNA干扰技术引入骨肉瘤基因治疗领域奠定了基础。

 
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