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neurite growth
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  轴突生长
     Growth-asscociated protein( GAP-43) is a kind of neuron-specific phosphoprotein that is mainly distributed in the membrane of axonal growth cones and presynaptic terminals, It is closely related with the development of the nervous system, nerve regeneration, neurite growth and the synaptic remodulation.
     生长相关蛋白(growth associated protein,GAP-43)是一种神经元特异性胞膜磷酸蛋白,主要分布于轴突生长锥质膜和突触前末端,与神经系统生长发育、再生、轴突生长和突触重组密切相关,对GAP-43的检测可以反应轴突的生长及突触的形成。
短句来源
     Neurite growth inhibiting proteins
     轴突生长抑制性蛋白
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     (ii) to promote axonal regeneration by application of neurotrophins (NTs) and/or blocking the neurite growth inhibitors;
     (ii)应用刺激神经生长的因子和/或阻断抑制轴突生长和延伸物质的作用,促进受损轴突的再生;
短句来源
     Some important genes facilitating to axon growth and guidance were only detected in the developmental period such as Efnb3, Ptn, Nrp, and Dbn1. Moreover, the expression of Rtn4, also called Nogo-A, which is the strongest blocking factor to axon extention of Nogo family, was found only in the injured groups. Nevertheless, Mdk, another key gene related to neuron migration and neurite growth, was found in both the developmental and injured groups.
     一些和轴突生长、导向、突触形成相关的基因Efnb3、Ptn、Nrp、Dbn1只在发育期间表达 ,抑制轴突生长的基因Rtn4只在损伤后表达 ,一个和神经元的迁移、轴突的生长相关的基因———Mdk在发育和损伤过程中均有高表达。
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     Conclusion BMMSCs could enhance the viability of neurons and prompt neurite growth.
     结论间充质干细胞能提高脊髓神经元的细胞活性,并能促进轴突生长
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  突起生长
     To investigate the effect of activin on neurite growth in dorsal root ganglia(DRG) and the gangliocyte survival as well as the relationship between activin function with nitric oxide release,dorsal root ganglia were collected from E8 chicken embryos and the growth of cultured DRG in vitro was observed by primary culture method.
     为了探讨激活素(activin)促进鸡胚背根神经节(dorsal root ganglia,DRG)突起生长、维持神经节细胞生存作用及其与一氧化氮(NO)释放的关系,实验采用8 d的鸡胚分离背根神经节,原代培养法,观察鸡胚背根神经节的体外生长情况。
短句来源
     Conclusion It is a favorable substrate that coated with PDL dissolved 0.01 mol/L by boric acid solution, and both the cell survival and neurite growth of sympathetic neurons can be improved by GDNF in vitro.
     结论用0.01mol/L硼酸溶解PDL包被是一种良好的生长底物处理方法,GDNF能促进离体培养的交感神经元的存活和突起生长
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     We use the crude extract from human fetal spinal cord to study the effect of endogenous substances of the spinal cord on neurite growth of E 12-15 mouse GABA-ergic and DNYergic neurons of the spinal cord in vitro.
     本文以体外培养的小鼠脊髓神经元为模型研究了人胚脊髓提取液对E 12—15小鼠脊髓中GABA能神经元和DNY能神经元的突起生长的营养作用,结果发现人胚脊髓提取液在蛋白浓度为250μg/ml时对GABA能神经元的突起生长无营养作用,但对DNY能神经元的突起生长有显著的促进作用。
短句来源
     Results In the group of cover glasses coated with PDL dissolved by 0.01 mol/L boric acid solution, cells grew dispersedly well, and both the cell survival and neurite growth were better than others.
     结果用0.01mol/L硼酸溶解的PDL进行包被,细胞存活和突起生长最好,且神经元分散好,优于其他各组;
短句来源
     The bioactivity of expressing protein was detected by tests of neurite growth of dorsal root knot of chicken embryo and tests of Brdu incorporation into PC12 cells.
     鸡胚背根神经节突起生长实验和小鼠嗜铬瘤细胞(mousea鄄drenalpheochromocytomacells,PC12)无血清培养生长刺激5溴-2-脱氧尿嘧啶(5-bromo-2-deoxyurididne,Brdu)掺入实验观察真核表达蛋白的生物学活性。
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  神经突起的生长
     The peak Ⅱ eluate and peak Ⅳ eluate obtained from the spinal cord tissue extract of morphine spared root rat through Sephacryl S 200 HR gel chromatography could promote the neurite growth of DRG.
     吗啡备用根大鼠脊髓组织提取液的SephacrylS 2 0 0HR凝胶层析Ⅱ峰洗脱液和Ⅳ峰洗脱液能够促进DRG神经突起的生长
短句来源
     Results NRF can promote the neurite growth of DRGs in vitro.
     结果免疫荧光细胞化学结果提示,NRF能促进背根神经节神经突起的生长,浓度为2.0 mg/L时生长状况最佳;
短句来源
     Results The extract of spinal cord tissue of spared root rat could enhance the neurite growth of chick embryonal dorsal root ganglion(DRG) in vitro.
     结果 备用根大鼠脊髓组织提取液能够促进体外培养的鸡胚背根节 (DRG)神经突起的生长 ;
短句来源
     This factor is a basic protein with a molecular weight of 11.5 kD and an isoelectric point of 9.39. It can promote the survival, neurite growth and 3H dopamine uptake of mesencephalic SN neurons of E14 rat brain in vitro. These effects are dose dependent, with optimal stimulation occurring at a concentration of 200ng/ml.
     该因子的分子量为11.5kD,等电点为9.39,它能促进体外培养的14d龄大鼠胚胎中脑黑质神经元的存活和神经突起的生长,以及对外源性多巴胺的摄取,其作用具有量依赖性关系,最适量为220μg/L
短句来源
     The peak Ⅳ eluate of gel chromatography was then furhter isolated by HPLC. It was observed. That the peak A eluate of HPLC could promote the neurite growth of DRG.
     应用HPLC对凝胶层析Ⅳ峰洗脱液作进一步的分离 ,发现HPLCA峰洗脱液能够促进DRG神经突起的生长
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  “neurite growth”译为未确定词的双语例句
     Hippocampi neurons were incubated with 160 μg/ml Ginkgolide B for 16 h and then 40 μg/ml 25-OH-cholesterol and 30 μg/ml apoE4 for 24 h to observe the activity of hippocampi neurons by MTT method and neurite growth and cell body by Image-Proplus analytic system.
     用160μg/ml的银杏内酯B处理海马神经元16h,40μg/ml25-OH-胆固醇和30μg/mlapoE4继续处理24h。 MTT比色实验观察海马神经元生长活力的改变;
短句来源
     RESULTS:When PC12 cells were cultured with ACM,compared with the control group , the vitality of PC12 cells was increased significantly(0.255±0.012 vs 0.510± 0.036,P< 0.001) ,but the neurite growth was not obvious in the experimental gro up.
     结果:①星形细胞条件培养液可增强PC12细胞活力(MTT测定的A值由0.255±0.012提高到0.510±0.036,P<0.001),却不能促使PC12神经元突起的生出。
短句来源
     Neuritin was a novel neurotrophic factor which was first reported by Nedivi as Candidate Plasticity-related genes15, on nature in 1993 ,then it was named by Israel scientist Naeve in 1997. Previous studies demonstrated that Neuritin can induce neurite growth, promote the outgrowth and arborization of neurite.
     神经突起因子,是一种新的神经营养因子,在1993年由美国麻省理工学院的Elly Nedivi在Nature杂志上首次作为可塑性相关候选基因(Candidate Plasticity-related genes,CPG15)报道,并于1997年由以色列科学家Naeve正式命名为Neuritin。
短句来源
     Objective: To study the relationship between fractal dimension and neurite growth of cultured rat dorsal root ganglions (DRGs) induced by different concentrations of nerve regeneration factor (NRF).
     目的:研究分维数与神经再生素(nerve regeneration factor,NRF)剂量促离体培养新生大鼠背根神经节(dorsal root ganglions,DRGs)神经突起生长之间的关系。
短句来源
     In order to study the effect on the neurite growth when Schwann cells express β-1,4-galactosyltransferase I (β-1,4-GalT- I ), the dorsal root ganglias(DRGs) were co-cultured with purified Schwann cells which were transfected with sense or antisense β-1, 4-GalT- I expression plasmid.
     为了探讨周围神经Schwann细胞中不同β1,4半乳糖基转移酶-1(β-1,4-GalT-I)表达水平对神经元轴突生长的影响,本实验首先构建了正、反义β-1,4-GalT-I表达质粒,然后将构建的不同浓度的正、反义表达质粒超表达于分离、纯化的大鼠Schwann细胞,最后将转染的Schwann细胞与分离的大鼠背根神经节共培养;
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  neurite growth
Effects of perinatal asphyxia on cell survival, neuronal phenotype and neurite growth evaluated with organotypic triple cultures
      
Ginsenosides Rb1 and Rg1 effects on survival and neurite growth of MPP+-affected mesencephalic dopaminergic cells
      
This indicates that cell adhesion molecules influence neuronal survival and neurite growth in co-cultures.
      
However, when physical contact between FLCs and neurons is prevented with membrane inserts, the DRG neurons exhibit a low survival and a deficient neurite growth.
      
In co-cultures with a membrane insert between skin-derived cells or 3T3 fibroblasts and DRG neurons few neurons survived and neurite growth was very sparse.
      
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Retinal basement membrane (RBM), also called inner limiting membrane of retina, is constituted by extracellular matrix. It was reported that neurite outgrowth of a neuron was closely related to extracellular matrix, particularly the laminin. In this laboratory RBM was used as the optimal substrate for retinal cells in culture.We have studied the surface of RBM and its relation to neurite outgrowth by scanning electronmicroscopy and immunogold transmission electronmicroscopy. RBM could be separated by mechanical...

Retinal basement membrane (RBM), also called inner limiting membrane of retina, is constituted by extracellular matrix. It was reported that neurite outgrowth of a neuron was closely related to extracellular matrix, particularly the laminin. In this laboratory RBM was used as the optimal substrate for retinal cells in culture.We have studied the surface of RBM and its relation to neurite outgrowth by scanning electronmicroscopy and immunogold transmission electronmicroscopy. RBM could be separated by mechanical disruption of the retina mounted between 2 adhesive substrata (membrane filter and poly-L-lysine coated glass). The surface of RBM studied was the side of RBM facing the optic fiber layer and ganglion cell layer.Small particles densely distributed on surface of RBM (Plate Ⅰ, Fig. 1 and 2) were shown to be chrysanthemum-like structures with radiative arms under the scanning electronmicroscopy (Plate Ⅰ, Fig. 3 and 4). The radiative arms on RBM of 12-day old chick embryo (E 12) were more in number and longer in length than that of the 6-day old chick embryo (E 6). The axons of ganglion cell from E 6 retinal strip extended out very well on RBM (Plate Ⅰ, Fig. 5). Growth cone was active with filopodia. The chrysanthemum-like structures changed to ball-particles when the RBM was cultured for 24 hr. Some of ball-particles lay over the growth cone, and some beside it. Over and beside the nerve fiber could also be seen some ball-particles. When many neurites grew on RBM, a lot of ball-particles were shown to be displaced and piled up (Plate Ⅰ, Fig. 6).The wholemount RBM labeled by indirect immunogold staining of Müller glial cell could be observed by transmission electronmicroscopy. The gold particles were located at the chrysanthemum-like structure of E 6 RBM (Plate Ⅱ, Fig. 7) and E 12 RBM (Plate Ⅱ, Fig. 8). It was suggested that those structures were the endfoots of Müller glial cells. Staining of PBS control or mouse serum control was negative (Plate Ⅱ, Fig. 9 and 10).The RBM labeled with indirect immunogold staining of laminin molecules showed that gold particles existed at and near the radiative arms of endfoots of Müller cells (Plate Ⅲ, Fig. 11 and 12). No particles could be seen at rabbit serum control on RBM (Plate Ⅲ, Fig. 13). However, after RBM cultured, the gold particles were distributed on the bottom of contracted endfoots of Müller glial cells and other area of RBM (Plate Ⅲ, Fig. 14).From these results we infered that laminincontained areas between endfoots of Müller glial cells on RBM might guide the neurite growth in vivo during retinal development.

扫描电镜观察到鸡胚视网膜基膜(RBM)上的菊花样结构,经免疫胶体金透射电镜证实为Müller胶质细胞的末端足,层粘连蛋白(laminin)分布在末端足的辐射臂及其周围的基膜上。培养的视网膜细胞,其神经纤维在RBM伸长,收缩了的Müller细胞末端足呈球状颗粒分布在神经纤维及其生长锥的上面和侧面。推测分布在Müller细胞末端足的辐射臂及其周围基膜上的层粘连蛋白在体内可能与神经纤维生长的导向有关。

The effects of the neurotrophic substance extracted from suckling pig brain and Cerebrolysin on cultured cerebral cortical netyons were investigated using the techniques of cultured cerebral cortical neurons and MTT colorimetric microassay. The results showed that suckling pig brain extraction could apparently promote neuronal growth and support neuron survival, The effect was dependent on dosage, the most effective dose of neurotrophic factors (NTFs) was 20mg/L. Cerebrolysin lacked the effect to promote neurite...

The effects of the neurotrophic substance extracted from suckling pig brain and Cerebrolysin on cultured cerebral cortical netyons were investigated using the techniques of cultured cerebral cortical neurons and MTT colorimetric microassay. The results showed that suckling pig brain extraction could apparently promote neuronal growth and support neuron survival, The effect was dependent on dosage, the most effective dose of neurotrophic factors (NTFs) was 20mg/L. Cerebrolysin lacked the effect to promote neurite growth and support neuron survival. MTT optical density (OD) results i-ndicated that suckling pig brain extraction could promote the activity of mitochondrion dehydrogenase in the cortical neurons. All the results showed that the extraction from suckling pig brain contained neurotrophic factors, which were not resulted only from pure nutrient effect of aminoacids or polypepti-des.

从乳猪脑中提取相应的神经营养物质,应用胎鼠大脑皮层细胞培养技术和四唑盐(M-TT)微量比色法,观察了乳猪脑提取物和脑活素(Cerebrolysin)对大脑皮层细胞培养的影响。结果表明,乳猪脑提取物能起明显促进神经元突起生长和支持存活的作用,并存在着剂量作用效应,当提取物剂量为20mg/L时作用效果最佳。脑活素对皮层神经元促突起生长和支持存活的作用不理想。MTT微量比色法结果显示,乳猪脑组织提取物能明显增强皮层神经元内线粒体脱氢酶的活力,并存在剂量相关效应。以上结果表明,乳猪脑提取物中含有神经营养因子(Neurotrophic Factors, NTFs)成分,并非由于氨基酸或多肽的单纯营养作用所致。

Abstract A mechanical injury model for the study of neural repair was created by dissecting the neuraxons of acoustic ganglion(AG)in chick (i,e. bilateral axotomy).Observations were made successfully on neurite growth in the injured AG by means of whole ganglion culture technique. The results demonstrated the outgrowth zone of AG in the earlier stages mainly consist of the non-neuronal cells-gliacytes and fibroblasts. At the 48 hours, newly formed neurites were found and the growth rate reached...

Abstract A mechanical injury model for the study of neural repair was created by dissecting the neuraxons of acoustic ganglion(AG)in chick (i,e. bilateral axotomy).Observations were made successfully on neurite growth in the injured AG by means of whole ganglion culture technique. The results demonstrated the outgrowth zone of AG in the earlier stages mainly consist of the non-neuronal cells-gliacytes and fibroblasts. At the 48 hours, newly formed neurites were found and the growth rate reached their maximum value within 72─96 hours. Until the end of culturing periods the survival condition of neurites or non-neuronal cells were well maintained in vitro. Our findings provide a direct evidence for the morphologi-cal repair by injured auditory nerve. It is reasonable to hypothesize based on these observa-tions that dissection of neuronal processes in AG was the trigger factor for the reparation and the nutrient medium supplied the exogenous promoting factors, while the events of prolifera-tion and migration of gliacytes and fibroblasts could have both intrinsic effects of guidance and neurotrophy on the process of the neurite regeneration.

应用神经组织培养技术,作者成功地观察到机械损伤后听神经纤维的轴突再生反应。选用正常10~16日龄WLS鸡,显微分离听神经节(AG),离断其双极神经突起后连续培养120小时。倒置显微镜下观察神经生长晕并测量其宽度。结果显示,神经胶质和纤维细胞构成培养早期生长晕的主要成分并可见细胞迁移。24小时可见轴突生长,具有双折光性。72~96小时增幅明显,120小时生长减缓。作者认为,机械分离致使听神经元双极突起断裂,由此诱发神经修复过程。合成培养基为轴突再生提供外源性促生长因子。AG组织中非神经元细胞的增殖与迁移对轴突再生,具有诱向和营养双重作用。本文为听神经损伤后的形态修复提供了直接的证据。

 
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