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definite cell
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  特定细胞
     Cell migration also occurs in chronic diseases such as cancer,atherosclerosis,chronic inflammatory fibrosis and so on,preventing the migration of definite cell could significantly inhibit the progression of these diseases.
     而在一些慢性疾病如癌症、动脉粥样硬化和慢性炎性纤维化等伴随着特定细胞迁移,阻止这些细胞迁移能抑制疾病进展。
短句来源
  “definite cell”译为未确定词的双语例句
     Establishment of the definite cell line of brain-derived neurotrophic factor gene-modified human bone marrow mesenchymal stem cells
     脑源性神经营养因子基因修饰人骨髓间充质干细胞有限细胞系的建立
短句来源
     Our study was characterized by: (1)definite cell source;
     目的:1.探讨细胞间粘附结构,细胞粘附分子及肌动蛋白分子在肿瘤细胞浸润、转移中的作用。
短句来源
     Exponentially growing cells were trypsinized, counted for the live ones and planted onto the cover glasses in definite cell number.
     取指数生长期细胞,胰酶消化计数活细胞数,按一定数量滴加单细胞悬液于盖玻片上,盖上培养皿上盖。
短句来源
     3. The Golgi apparatus may be regarded as a product of food assimilation, not a definite cell organ.
     俟三十六小时後再餵,卽有许多小粒在细胞前端边缘或卽係高基體 (3)由此结果可知高基體之多寡,既视绝食与否而定,或可谓为食物同化之产物,非固定之细胞器官也。
短句来源
  相似匹配句对
     Our study was characterized by: (1)definite cell source;
     目的:1.探讨细胞间粘附结构,细胞粘附分子及肌动蛋白分子在肿瘤细胞浸润、转移中的作用。
短句来源
     Definite-purpose motor of induction cell and spiral coil
     螺旋形线圈感应电池的专用电动机
短句来源
     Fuel Cell
     燃料电池
短句来源
     cell morphology;
     细胞形态;
短句来源
     The answer is definite.
     答案是肯定的。
短句来源
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  definite cell
Definite cell loss in the medial aspect, of the most rostral third of Rtp is detectable after cerebellar hemisection involving parts or the entire depth of sublobule VIb.
      
The relationship between the drug concentration and exposure time of neocarzinostatin (NCS) for a definite cell-killing effect was kinetically analyzed, taking into consideration its loss in biological activity during incubation.
      
We assume that these hydrolases modified definite cell wall regions transforming them into 'canals'.
      
The immediate onset of PS deprivation and the PS recovery, despite the definite cell loss, suggests a mechanism independent of cell destruction.
      
Some are specific for a definite cell type, while others detect endocrine differentiation in general.
      
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1. The albino mice were used for this study. The Golgi apparatus in the cells of the epididymis and the vas deferens of normal, starved, and refed animals were studied.2. The amount of the Golgi elements can be maintained by feeding, not only by the sex hormone.3. The Golgi apparatus may be regarded as a product of food assimilation, not a definite cell organ.4. It is suggested that the Golgi apparatus has similar organ with that of mitochondria and secretion granules, but with different quantity of lipoids....

1. The albino mice were used for this study. The Golgi apparatus in the cells of the epididymis and the vas deferens of normal, starved, and refed animals were studied.2. The amount of the Golgi elements can be maintained by feeding, not only by the sex hormone.3. The Golgi apparatus may be regarded as a product of food assimilation, not a definite cell organ.4. It is suggested that the Golgi apparatus has similar organ with that of mitochondria and secretion granules, but with different quantity of lipoids.

(1)本研究以白鼠为材料,就常态白鼠绝食及再餵之白鼠观察其副睾丸及输精管上皮细胞内之高基體。查得在绝食期内副睾上皮细胞之高基體逐渐減少,由网状體变为颗粒,先集于前端後卽散开,至三十六小时高基體绝少,再餵後復有颗粒出现於前端。 (2)输精管上皮细胞内高基體原作线状,列於细胞前部。绝食十三时後,变为颗粒。俟三十六小时後再餵,卽有许多小粒在细胞前端边缘或卽係高基體 (3)由此结果可知高基體之多寡,既视绝食与否而定,或可谓为食物同化之产物,非固定之细胞器官也。 (4)高基體之起源或与粒线體,及分泌粒同,但其所含类脂質之量不同。

To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease Methods Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT 4 cells, and measured by flow cytometry Results We developed a cyclin E+A/DNA flow cytometry analysis method, which may distinguish G 0, early G 1, late G 1, S, G 2 and M phase cells, rather than three phases in the DNA content histogram Conclusion Cyclin E+A/DNA multiparameter flow cytometry can simultaneously differentiate...

To establish a new method for analyzing the cell cycle in tumor which is a kind of cell cycle disease Methods Mixtures of cyclin E and cyclin A antibodies were incubated with fixed MOLT 4 cells, and measured by flow cytometry Results We developed a cyclin E+A/DNA flow cytometry analysis method, which may distinguish G 0, early G 1, late G 1, S, G 2 and M phase cells, rather than three phases in the DNA content histogram Conclusion Cyclin E+A/DNA multiparameter flow cytometry can simultaneously differentiate in the same sample six cell groups: G 0, early G 1, late G 1, S, G 2 and M phase cells It performed better than any other cell cycle analysis methods that we have used and has a definite cell biology foundation

目的 越来越多的资料表明肿瘤是一类细胞周期性疾病 ,从而使得细胞周期分析在细胞生物学 ,肿瘤生物学领域显得格外重要。迄今为止 ,有很多方法应用于细胞周期分析中 ,但这些方法因为这样或者那样的缺点已无法适应今天的细胞周期分析。本研究的目的是建立一种新的 ,更为合理的细胞周期分析方法。方法 将cyclinE以及cyclinA的单克隆抗体按一定的比例混合后与固定了的MOLT 4细胞孵育 ,后用流式细胞仪检测。结果 我们发明的CyclinE +A/DNA多参数流式细胞术能将细胞分为六个细胞群体 :G0 、早G1、晚G1、S、G2 、M期细胞。而在DNA含量直方图仅能区分三个细胞群体 :G0 /G1、S、G2 /M期细胞。结论 CyclinE +A/DNA多参数流式细胞术能同时在同一样本中将细胞分为六个细胞群体 :G0 、早G1、晚G1、S、G2 、M期细胞。其对细胞周期分析明显优于目前采用的细胞周期分析方法 ,并且有明显的生物学基础。

Objective:To investigate the availability for rhIL-18 in the in vitro culture system to stimulate PBMHCs,with inducing cytotoxicities against tumor cells and to analyze the influencing factors for the effects.Methods:The NK cells,T cells and dendritic cells were separated from fresh PBMNCs by using the Stem Sep TM immunomagnetic beads.Cell phenotypes of the purified cell populations were identified with FCM technique.The cell co-culture system was established as follows.The PBMNCs or the cell preparations deleting...

Objective:To investigate the availability for rhIL-18 in the in vitro culture system to stimulate PBMHCs,with inducing cytotoxicities against tumor cells and to analyze the influencing factors for the effects.Methods:The NK cells,T cells and dendritic cells were separated from fresh PBMNCs by using the Stem Sep TM immunomagnetic beads.Cell phenotypes of the purified cell populations were identified with FCM technique.The cell co-culture system was established as follows.The PBMNCs or the cell preparations deleting the definite cell subset with immunoscreening were co-cultivated with mitotic-inactivated tumor cells in the presence of rhIL-18.Cytotoxicities of the various effector cell preparations to a series of tumor cell lines were examined by the isotope releasing assay.Results:In the in vitro cell co-culture system,rhIL-18 rapidly induces activation of the cytolytic responses against various tumor cell lines mediated by PBMNCs.The rapid induction within 24 h of culture was dependent on the dosage of rhIL-18,with the optimal dose of 100 ng/ml of the cytokine.The activated cytotoxicities were abrogated by deleting NK cells prior to the cell co-culture but did not vary when either T cells or DCs were removed.The cytotoxic responses were shown as a pattern of broad-spectrum to the target cells used and were not blocked by anti-MHC moAbs.Conclusion:NK cells were responsible for the rapid induction of cytotoxicities against tumor cells by rhIL-18.

目的 :应用rhIL 18在体外培养系统 (Coculturesysteminvitro ,CCs)中诱导快速肿瘤杀伤效应 ,并对这种快速杀伤效应的影响因素进行了探讨。方法 :采用StemSepTM 免疫磁性细胞分离法分离人外周血NK细胞、T细胞及树突状细胞 (DCs) ,流式细胞仪分析细胞表型 ,1 2 5I UdR标记的细胞毒实验检测杀伤活性。结果 :rhIL 18在CCs中能够诱导快速肿瘤杀伤效应 ,这种杀伤效应与rhIL 18含量呈剂量依赖关系 ,DCs和T细胞的存在与否对这种杀伤效应无明显影响 ,同时这种快速杀伤效应无抗原特异性、不受MHC的限制。结论 :在体外培养系统中只要有rhIL 18和NK细胞的存在 ,即能产生对肿瘤细胞的快速杀伤效应 ,即这种快速杀伤效应细胞为NK细胞。

 
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