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provide cell
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  提供细胞
     This will provide cell lines for in vitro experiments and possibly for cell banks for RPE transplantation for some fundus diseases.
     这可提供细胞系,方便了体外实验,并可能作为RPE细胞移植治疗一些眼底病的细胞库。
短句来源
     bjective\ To provide cell kinetics characteristics of the SME1 cell,which derived from the latedeveloped embryo of southern catfish(Silurus meridionalis Chen),and give some theoritic advice on embryonic development of the southem catfish as well.
     目的SME-1细胞系来源于南方大口鲶(SilurusmeridionalisChen)的晚期胚胎,通过对其细胞周期的分析,可为该细胞系提供细胞动力学特征,同时也可为胚胎发育提供理论基础。
短句来源
     Objective:To establish the cell model expressing genome of HBV-YVDD mutation strain and to provide cell model for studying the characteristic of HBV-YVDD mutation strain and screening anti-virus medicine.
     目的:建立能稳定表达乙肝病毒(HBV)YVDD变异株的细胞模型,为研究HBV YVDD变异株的生物学特性和进行抗病毒药物筛选提供细胞模型。
短句来源
     Immunohistochemical staining show those endothelial cells were Ⅷ factor positive. Conclusion Vascular endothelial cells cultured provide cell source for the research of vascular tissue engineering.
     结论兔血管内皮细胞培养成功,本法可为组织工程血管的研究提供细胞来源。
短句来源
  “provide cell”译为未确定词的双语例句
     [Objective]: 1) Obtain the smooth muscle cells(SMCs) of canine' s blood vessels by cell culture technique to provide cell resources for the experimental study on artery tissue engineering.
     [目的] 1)运用细胞培养技术获取犬的血管平滑肌细胞,并培养扩增,为组织工程动脉的构建提供种子细胞;
短句来源
     The present study aimed at: (1) to establish a method of cryopreservation of human RPE and cell culture from thawing of frozen cells in order to provide cell banks to be used for cell transplantation in corresponding diseases;
     本研究旨在建立一种人RPE细胞冻存和复苏的方法,为临床上开展RPE移植以治疗相关眼病提供可能的细胞库;
短句来源
     The aim is to establish rat alveolar type II epithelial cell line and to provide cell models for epithelial cell cancer research.
     建立肺泡II型细胞系为研究细胞恶性转化提供实验模型。
短句来源
     AIM: To explore the condition and approach for the culture of primary cardiac myocyte, and establish an effective method for cardiac myocyte culture, so as to provide cell resource for cardiovascular related study in tissue engineering.
     目的:探索原代心肌细胞培养的条件和方法,建立一套高效快捷的原代心肌细胞培养方案,为组织工程心血管相关研究提供心肌细胞来源。
短句来源
  相似匹配句对
     Stem cell technology could provide an answer.
     干细胞技术的出现为解决这一难题提供了新的手段。
短句来源
     To provide the evidence for the mechanism of arsenic induced cell transformation.
     通过上述研究为砷诱导细胞转化的分子机制提供依据。
短句来源
     penetration into the cell;
     病毒穿透细胞膜,进入细胞;
短句来源
     Cytotoxic T Cell
     细胞毒T细胞
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     Weinig Provide More
     威力提供更多
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  provide cell
Although reactive oxygen species (ROS) increased in response to TA, this did not seem to play a pivotal role in the apoptotic process since different anti-oxidants were unable to provide cell protection.
      
Serum coating of microcarriers could provide cell attachment factors and may replace costly attachment factors supplemented in low serum medium and serum-free medium.
      
In antidiuresis and diuresis the sum of intracellular cations (sodium and potassium), accompanying anions and urea was insufficient in both cases to provide cell osmolatities similar to those in extracellular compartments.
      
Under the agreement, the two companies will provide cell-phone technology as well as equipment to the Afghan Telecom Company.
      
The goal of the current study was to examine the accuracy of the extended combined model's ability to provide -cell insulin secretion rates in vivo.
      
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Three different mutagenic pathways induced by ultraviolet irradiation can be distinguished in human cells by use of parvoviral probe. Direct mutagenesis occurs as a response of the cellular constitutive replication machinary (or repair function) to lesions in viral DNA. Indirect untargeted mutagenesis is conditioned on providing cells an inducible error-prone repair function which replicates viral DNA less accurately. Indirect targeted mutagenesis is also conditioned on a cell-inducing treatment,...

Three different mutagenic pathways induced by ultraviolet irradiation can be distinguished in human cells by use of parvoviral probe. Direct mutagenesis occurs as a response of the cellular constitutive replication machinary (or repair function) to lesions in viral DNA. Indirect untargeted mutagenesis is conditioned on providing cells an inducible error-prone repair function which replicates viral DNA less accurately. Indirect targeted mutagenesis is also conditioned on a cell-inducing treatment, but is directed at special lesions in viral DNA. Within the low dose range, the indirect untargeted mutagenesis plays a main role in the entire mutagenic charge loaded on cells by certain environmental genotoxic agents. Certain DNA lesions, apurinic sites, the secondary DNA lesions induced by alkylating agents, for example, are the proper substrates of the inducible error-prone repair activity.

用细小病毒H-1为探针,可在人细胞中检测由紫外线照射诱导的三种不同致突变途径.直接突变产生于细胞的结构性复制(或修复)功能对受损DNA模板的复制;间接无靶突变和间接有靶突变分别产生于紫外线诱导的易错修复功能对完整模板和模板上的靶损伤的作用.在低剂量范围内,间接无靶突变在某些DNA毒剂诱导的细胞突变中起主要作用.某些DNA损伤,如烷化剂诱导的次级损伤(无碱基位损伤)可作为诱导性易错修复功能的靶损伤.

PURPOSE:To establish methods for cryopreservation of human retinal pigment epithelial cells(RPEs)and cell culture from thawing of frozen cells.METHODS:Primary cultured RPEs or its first or second passages,added with 10% dimethylsulfoxide,were kept in -20℃ for 1 to 2 hours,and then further froze to -40℃ over night before being placed in liquid nitrogen.The frozen cells were thawed in 60℃ within 2 minutes.Trypan blue staining ...

PURPOSE:To establish methods for cryopreservation of human retinal pigment epithelial cells(RPEs)and cell culture from thawing of frozen cells.METHODS:Primary cultured RPEs or its first or second passages,added with 10% dimethylsulfoxide,were kept in -20℃ for 1 to 2 hours,and then further froze to -40℃ over night before being placed in liquid nitrogen.The frozen cells were thawed in 60℃ within 2 minutes.Trypan blue staining and immunocytochemical staining with anti human keratin were performed for cell viability and differentiation.The growth curve was also determined by calculating the total number of cells/well/day.RESULTS:The viable rate from frozen RPEs was 90%.No differences were observed for growth activity between cultures from frozen cells and controls.The cells were positive with anti human keratin staining.The logarithmic growth phase was during 1 to 4 days and the doubling time was 1.55 days.CONCLUSION: Cryopreservation of RPEs in liquid nitrogen can maintain biological activities of cells with normal growth and features after thawing.This will provide cell lines for in vitro experiments and possibly for cell banks for RPE transplantation for some fundus diseases.

目的:建立人视网膜色素上皮(retinalpigmentepithelium,RPE)细胞的冻存和复苏方法。方法:根据慢冻速融的细胞冻存原则,将原代或传代1~2次培养的人RPE细胞,加入10%二甲基亚砜作为保护剂,液氮中冻存。复苏时将冻存细胞在60℃、2分钟内融化,用台盼蓝染色测定细胞存活率,用抗人角蛋白抗体免疫细胞化学染色作细胞鉴定,每日计算细胞数以确定细胞生长曲线。结果:经冻存复苏后的RPE细胞的存活率达90%,生长状态与对照组无差异,抗人角蛋白染色阳性,细胞对数生长期在1~4天,群体细胞倍增期约为1.55天。结论:人RPE细胞液氮冻存可保持生物活性,复苏后生长良好,保持了细胞特征。这可提供细胞系,方便了体外实验,并可能作为RPE细胞移植治疗一些眼底病的细胞库。

bjective\ To provide cell kinetics characteristics of the SME1 cell,which derived from the latedeveloped embryo of southern catfish(Silurus meridionalis Chen),and give some theoritic advice on embryonic development of the southem catfish as well.\ Methods\ To analysis cell cycle by means of flow cytometry(FCM).\ Results\ The results suggest the percentage of G 1,S,G 2/M phase cell is 70.1%~85.6%,12.2%~25.3% and 1.6%~4.6%,respectively.\...

bjective\ To provide cell kinetics characteristics of the SME1 cell,which derived from the latedeveloped embryo of southern catfish(Silurus meridionalis Chen),and give some theoritic advice on embryonic development of the southem catfish as well.\ Methods\ To analysis cell cycle by means of flow cytometry(FCM).\ Results\ The results suggest the percentage of G 1,S,G 2/M phase cell is 70.1%~85.6%,12.2%~25.3% and 1.6%~4.6%,respectively.\ Furthermore,with the advantage of passage,G 2/M phase cell gets higher percentage.\ Conclusion\ The results of cell cycle distribution suggest that the SME1 cell is much more proximal to normal cell.

目的SME-1细胞系来源于南方大口鲶(SilurusmeridionalisChen)的晚期胚胎,通过对其细胞周期的分析,可为该细胞系提供细胞动力学特征,同时也可为胚胎发育提供理论基础。方法应用流式细胞光度术分析SME-1细胞系的细胞周期分布特点。结果在不同代龄的细胞群体中,70.1%~85.6%的细胞滞留于G1相,12.2%~25.3%的细胞进行活跃的DNA复制(S相),处于M相的细胞有1.6%~4.6%,而且细胞随着传代代龄的增加,G2/M相细胞的比例加大。结论SME-1细胞的周期分布特点表明它更接近于未经转化的正常细胞

 
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