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     Objective The present study was conducted to find out the infection situation and the risk factors of HIV among drug addicts in Qingyuan City so as to provide a scientific basis for the intervention, prevention and control of the prevalence of AIDS.
     目的了解清远市吸毒人群HIV感染情况及危险因素,为开展干预工作,预防控制艾滋病的流行提供科学依据。
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     Conclusion RSV is still the commonest pathogeny of the infants with acute respiratory tract infection in the island area, and the diagnosis of viral etiology can provide a reliable basis for clinical diagnosis and treatment.
     结论RSV感染仍是海岛婴幼儿急性呼吸道病毒感染最常见的病原,病毒病原的诊断可为临床诊断和治疗提供可靠的依据。
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     This paper displays control technique of the noise from Passiom 12K device,according to the measuring result,the noise reason of the device was determined,and provide a foundation for the corresponding noise control design.
     主要系统的介绍了Passiom 12K噪声的控制技术,根据测量的结果对噪声进行了分析,为相应的噪声控制提供了合理的依据。
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     Apply competence model to the organization career planning,it provide a new method and tool for career planning.
     把胜任特征模型导入组织职业生涯规划,为我们科学开展员工生涯规划提供了一种新的方法与工具。
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     In this article,from the point of modern equipment management,does a systemic research on key equipment technology of HSM,which provide a techical reference for design,selection,application and equipment management of HSM.
     从现代设备管理的角度出发,针对高速加工的关键设备技术进行了系统的研究,为在设计、选择、应用及管理HSM设备方面提供新的技术参考。
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     This paper displays control technique of the noise from Passiom 12K device,according to the measuring result,the noise reason of the device was determined,and provide a foundation for the corresponding noise control design.
     主要系统的介绍了Passiom 12K噪声的控制技术,根据测量的结果对噪声进行了分析,为相应的噪声控制提供了合理的依据。
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     Apply competence model to the organization career planning,it provide a new method and tool for career planning.
     把胜任特征模型导入组织职业生涯规划,为我们科学开展员工生涯规划提供了一种新的方法与工具。
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     Conclusion The humanized antibody 813-F(ab)2 can strongly bind to the platelets of human,macaque and dog. Thus,it will provide a basis for its pharmacodynamics estimation and toxicological analysis
     结论813-F(ab)2与人、猕猴和毕格犬的血小板均有较强的特异性结合能力,为813-F(ab)2的后续药效学评价、毒理分析等提供了基础。
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     CONCLUSION: ① Slight hypoxia can promote the proliferation of rat skeletal myoblasts and provide a new way for abundantly cultivating cells in vitro.
     结论:①轻中度低氧可以促进大鼠骨骼肌成肌细胞的增殖,为体外大量扩增细胞提供了新思路。
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     Conclusions PE not only provide a effective therapeutic measure for severe hepatitis,but also increase their livability.
     结论PE为重型肝炎治疗提供了有效的手段,大大提高了疗效,但应把握治疗时机和治疗次数,及早治疗,严密观察病情变化,使病人得到及时救治。
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     Common Object Request Broker Architecture provide a new chance of development of network management.
     公共对象请求代理体系结构(Common Object Request Broker Architecture,CORBA)的出现为网络管理提供了一个新的发展机遇。
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     A new standard, the Common Information Model for Power Systems (CIM) has been developed to provide a generic model of a power system.
     电力系统公共信息模型CIM(Common Information Model)提供了一个普遍的电力系统模型。
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     With the development of the technology of computer and grid, and the constant advance of manufacturing informationization, Manufacturing Grid( MG ) emerges as the results of combining the information technology and manufacturing, to provide a general platform for Networked-Manufacturing.
     随着计算机技术、网格(Grid)技术的发展,制造业信息化的深入,制造网格(Manufacturing Grid,MG)作为信息技术和制造技术结合的产物应运而生,为网络化制造提供了一个通用的协作平台。
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     Practicing activities in Chinese provide a true occasion for students to communicate, which help them gain the experience of practice and improve their abilities for resolving problems.
     语文实践活动为学生提供了一个与现实生活、与他人交往的情境,使学生在历练中积累经验和体验,提高解决问题的能力,因而语文实践活动是语文素养养成的重要途径。
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     The digital signal processor(DSP) system based on serial network interfacing controller(SNIC) can communicate with PC quickly and accurately, thus to provide a network programming interface hiding the physical details for users.
     基于串行网络接口控制器 SNIC 的数字信号处理器 DSP 系统可以与 PC进行高速准确的通信 ,从而为 DSP系统用户提供了一个将物理网络细节隐藏起来的网络编程接口
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     [Conclusion] The serum β-HCG at 12-14 days after IVF-ET is an effective predictor for pregnancy outcome,which may provide a reference for clinical counseling and management.
     [结论]IVF-ET后12~14d血清β-HCG是早期预测妊娠结局的有效指标,可供正确指导咨询、及时处理参考。
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     116(2004)and GB 12981-2003 respectively,so as to provide a reference for the students and users.
     116(2004)及我国的强制性国家标准GB 12981-2003进行了对比,供研究和使用者参考。
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     Objective To study the peak intensity in contrast ultrasonography of benign prostatic hyperplasia(BPH) and its relationship with angiogenesis and to provide a woundless index for angiogenesis evaluation in clinic.
     目的探讨良性前列腺增生(benign prostatic hyperplasia,BPH)的超声造影峰值强度与血管生成的相关性。
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     PCI2040 is applied to provide a tried connection between TMS320VC5410A DSP and PCI bus to guarantee the acquired data transferred to PC in high speed and high efficiency.
     应用PCI2040实现TMS320VC5410A DSP到PCI总线间可靠连接,从而保证了采集数据快速、高效地传输到PC机。
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     Conclusion The pENTRTM/U6-p498 siRNA expression vector can specifically and efficiently inhibit 4-1BB gene expression,which may provide a foundation to construct 4-1BB Lentivirus and study 4-1BB gene function in mouse T lymphocytes.
     其余siRNA载体,几乎无干扰效应,与无关干扰组比较,差异无统计学意义(P>0.05)。 结论我们所构建的p498 siRNA真核表达载体,能明显干扰m4-1BB蛋白的瞬时表达,可望用于m4-1BB Lentivirus干扰表达系统的构建,进一步在小鼠原代T淋巴细胞内实现高效4-1BB表达沉默,以深入研究4-1BB对T淋巴细胞的生物学影响。
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We provide a direct computational proof of the known inclusion ${\cal H}({\bf R} \times {\bf R}) \subseteq {\cal H}({\bf R}^2),$ where ${\cal H}({\bf R} \times {\bf R})$ is the product Hardy space defined for example by R.
      
We furthermore provide a necessary and sufficient condition for a band-limited function g(t) generating a Weyl-Heisenberg frame for L2 (?) to have a band-limited minimal dual γ0 (t).
      
This somewhat technical result does provide a method for simple constructions of low pass filters whose only smoothness assumption is a Holder condition at the origin.
      
These examples have an interpretation in the setting of radial functions on Rd and zonal functions on compact two-point homogeneous spaces, where they provide a new transform which possesses many properties of the classical Gabor transform.
      
We provide a general method to derive Banach frames and atomic decompositions for these Banach spaces by sampling the continuous frame.
      
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(1) Sodium salt of reduced codehydrogenase I has been obtained in good yield as a dry powder from codehydrogenase I by reduction with alcohol and alcohol dehydrogenase. This preparation was stable for at least 5 months when kept dry at -15℃. (2) The properties of the particle-bound codehydrogenase I cytochrome reductase system in heart muscle preparation were found to differ considerably from those of the soluble enzyme as obtained by Mahler et al. Among other things, the affinity for cytochrome c of the particle-bound...

(1) Sodium salt of reduced codehydrogenase I has been obtained in good yield as a dry powder from codehydrogenase I by reduction with alcohol and alcohol dehydrogenase. This preparation was stable for at least 5 months when kept dry at -15℃. (2) The properties of the particle-bound codehydrogenase I cytochrome reductase system in heart muscle preparation were found to differ considerably from those of the soluble enzyme as obtained by Mahler et al. Among other things, the affinity for cytochrome c of the particle-bound enzyme is much greater than the soluble enzyme. The Michaelis constant for cytochrome c of the former is only one twelfth of that of the latter.(Fig. 2A). (3) With either oxygen or excess cytochrome c as electron acceptor, it was found that the overall activity, in terms of rate of oxygen consumption or cytochrome c reduction, when both succinate and reduced codehydrogenase I were oxidized simultanously, did not represent the sum of the rates of oxidation when these two substrates were separately oxidized but equalled only the faster of the two separate oxidation rates(Fig. 5, Tables 1, 2). If 2,6-dichlorophenol indophenol was used as the electron acceptor, the overall rate of simultaneous oxidation of these two substrates was found to equal exactly the sum of the rates of separate oxidation(Table 3). (4) When either oxygen or excess cytochrome c was used as the electron acceptor, reduced codehydrogenase I and succinate each inhibited the rate of oxidation of the other(Figs 4, 6 & 7). Evidence has been presented to show that the inhibition of succinate oxidation by reduced codehydrogenase I is not due to the accumulation of oxaloacetate. (5) When malonate was also added to the reaction mixture, succinate no longer produced any inhibition of the oxidation of reduced codehydrogenase I(Fig. 8). (6) It is therefore concluded that in heart muscle preparation both succinate and reduced codehydrogenase I are oxidized by cytochrome c through a common, velocity limiting factor. This is in accordance with the view previously reached by some workers from studies on the action of certain inhibitors. However, it should be noted that in our experiments no agents which might produce any conceivable change in the colloidal structure of the enzyme system has been employed. (7) It should be emphasized that our results clearly show that great caution must be exercised in drawing conslusion on the role an enzyme might play in a complex enzyme system from studies of the properties of a solubilized enzyme. (8) It is believed that the competition of two enzyme systems for a common linking factor as demonstrated in this report has provided a new method for studies on the mutual relations of two or more enzyme systems.

(一)本報告提供了一個從輔酶Ⅰ,用酶還原法製備還原輔酶Ⅰ的方法。我們所製得的還原輔酶Ⅰ鈉鹽乾粉,可以在低温保存數月而不被氧化。 (二)與心肌製劑中顆粒相結合的輔酶Ⅰ細胞色素還原酶系,和用乙醇抽出的水溶性的輔酶Ⅰ細胞色素還原酶的性質頗不相同。其中比較重要的不同點是對於細胞色素c的親力,前者遠大於後者,其米氏常數僅約為後者的十二分之一。 (三)用一心肌顆粒製劑作為材料,無論用氧或過量之細胞色素c作為氫受體,還原輔酶Ⅰ與琥珀酸同時氧化時的總速度,不等於二者分別氧化時速度之和,而僅等於其中氧化較快者單獨氧化時之速度。但如用[2,6]二氯靛酚作為氫受體時,二者共同氧化時之總速度完全等於二者分別氧化時速度的和。 (四)當用氧或過量之細胞色素c作為氫受體時,琥珀酸與還原輔酶Ⅰ能彼此互相抑制對方氧化的速度。有足夠的實驗材料說明,還原輔酶Ⅰ對於琥珀酸氧化的抑制,不是由於草醯乙酸聚集的緣故。 (五)如果在反應混合物中同時含有琥珀酸脫氫酶的專一抑制劑,丙二酸,則琥珀酸對於還原輔酶Ⅰ氧化作用的抑制即被解除。 (六)根據以上的實驗結果,可以認為,還原輔酶Ⅰ及琥珀酸先通過一個共同的因子與細胞色素c作用。這個共同的因子在一般情形之下,也是...

(一)本報告提供了一個從輔酶Ⅰ,用酶還原法製備還原輔酶Ⅰ的方法。我們所製得的還原輔酶Ⅰ鈉鹽乾粉,可以在低温保存數月而不被氧化。 (二)與心肌製劑中顆粒相結合的輔酶Ⅰ細胞色素還原酶系,和用乙醇抽出的水溶性的輔酶Ⅰ細胞色素還原酶的性質頗不相同。其中比較重要的不同點是對於細胞色素c的親力,前者遠大於後者,其米氏常數僅約為後者的十二分之一。 (三)用一心肌顆粒製劑作為材料,無論用氧或過量之細胞色素c作為氫受體,還原輔酶Ⅰ與琥珀酸同時氧化時的總速度,不等於二者分別氧化時速度之和,而僅等於其中氧化較快者單獨氧化時之速度。但如用[2,6]二氯靛酚作為氫受體時,二者共同氧化時之總速度完全等於二者分別氧化時速度的和。 (四)當用氧或過量之細胞色素c作為氫受體時,琥珀酸與還原輔酶Ⅰ能彼此互相抑制對方氧化的速度。有足夠的實驗材料說明,還原輔酶Ⅰ對於琥珀酸氧化的抑制,不是由於草醯乙酸聚集的緣故。 (五)如果在反應混合物中同時含有琥珀酸脫氫酶的專一抑制劑,丙二酸,則琥珀酸對於還原輔酶Ⅰ氧化作用的抑制即被解除。 (六)根據以上的實驗結果,可以認為,還原輔酶Ⅰ及琥珀酸先通過一個共同的因子與細胞色素c作用。這個共同的因子在一般情形之下,也是這兩個酶系統的速度限制因子。應該指出在我們的實驗中,並未使用任何可能影響酶系統結構的條件,因此我們的結果是在一個比較接近於生理狀態的情形之下獲得的。 (七)應該着重指出,從本報告的結果可以看到,一個用人為的方法從複雜酶系上溶解下來的酶的性質,有時並不能代表這個酶在有組織的酶系統中的真實情况。 (八)我們相信,本報告所說明的兩酶系競爭一個共同因子的一些現象,將为研究複雜酶系之間的相互關係,提供一個新的方法。

Sham feeding was performed on esophageal fistula dogs with normal mixed food. The change of blood sugar concentration was followed for 3 hours as an indication of insulin secretion. The results were as follows: (1) After 10-15 minutes of sham feeding, the blood sugar showed marked fluctuations in most of the experiments and a slight decrease in some of the experiments as compared with that in the control experiments without sham feeding. (2) After inactivation of the adrenal medulla(removal of right adrenal...

Sham feeding was performed on esophageal fistula dogs with normal mixed food. The change of blood sugar concentration was followed for 3 hours as an indication of insulin secretion. The results were as follows: (1) After 10-15 minutes of sham feeding, the blood sugar showed marked fluctuations in most of the experiments and a slight decrease in some of the experiments as compared with that in the control experiments without sham feeding. (2) After inactivation of the adrenal medulla(removal of right adrenal and denervation of left adrenal), a marked fall of blood sugar was observed after sham feeding. The lowered blood sugar level maintained for about 2 hours and then returned to normal gradually. (3) The effect in(2) was abolished after injection of hyoscyamine, an atropinelike drug, or by subdiaphragmatic vagotomy. (4) Further observations were made on dogs with denervated pancreas after the inactivation of the adrenal medulla. No fall of blood sugar was also observed after sham feeding. Thus, the fall of blood sugar elicited by sham feeding was produced reflexly through the vago-insulin system. (5) These observations provided a further evidence for the nervous control of insulin secretion and gave rise to a favourable means for studying the significance of the vago-insulin system under natural conditions. We wish to express our gratitude to Comrade for bis valuable advice and to Prof. T. C. Shen for his constant encouragement throughout this work.

本工作用具有食道瘻的狗,用正常混合食物進行假飼,觀察血糖濃度在三小時內的變動,作為胰島素分泌的指標,結果如下: (一)正常假飼後,血糖濃度與不進行假飼的對照實驗比較,呈顯著的波動,有的血糖水平呈降低現象。 (二)取消腎上腺髓質作用後,再進行假飼,血糖濃度呈顯著的下降,並持續約兩小時左右,以後才逐漸恢復,說明取消腎上腺髓質作用後,假飼可引起顯著的血糖降低。 (三)用取消腎上腺體質作用的狗,再應用阿托平類藥物,或切斷其膈下迷走神經後,進行假飼,則不再有血糖降低,這說明引起血糖下降是通過迷走神經的。 (四)當切斷胰腺的一切外來神經後,再將取消了腎上腺髓質的狗進行假飼,血糖水平也不再下降,說明假飼所引起的血糖下降是由於反射性地通過迷走神經,興奮胰島裝置釋放胰島素對機體作用的結果。 (五)這項結果可作為神經因素影響胰島裝置的一個確切的證據,並對於闡明在自然條件下迷走神經—胰島素系統的重要意義,提供了有利的條件。

(1) Cytochrome c containing 0.43% iron has been obtained from beef, pig or horse heart muscles by direct adsorption of the neutralized trichloroacetic acid extracts of heart muscle on the cation exchanger"synthetic zeolite" followed by elution with 3.84 M ammonium sulfate and precipitation with 20% trichloroacetic acid. This provides a simple method for large-scale preparation of pure cytochrome c. (2) The ratio of optical densities at 550 mμ(reduced) and 278 mμ of 0.43% iron cytochrome c varies, with...

(1) Cytochrome c containing 0.43% iron has been obtained from beef, pig or horse heart muscles by direct adsorption of the neutralized trichloroacetic acid extracts of heart muscle on the cation exchanger"synthetic zeolite" followed by elution with 3.84 M ammonium sulfate and precipitation with 20% trichloroacetic acid. This provides a simple method for large-scale preparation of pure cytochrome c. (2) The ratio of optical densities at 550 mμ(reduced) and 278 mμ of 0.43% iron cytochrome c varies, with its degree of reduction, from 1.13 to 1.26. The average ratio of our preparations is 1.23. (3) The absorption spectra(230-600 mμ) of oxidized and reduced cytochrome c have been measured. The molecular extinction coefficient at 550 mμ of oxidized, 0.43% iron, cytochrome c is 0.80×10~4. This value differs considerably from that reported in the literature. (4) Some enzymic properties of cytochrome c containing 0.43% iron are compared with those of a preparation containing 0.34% iron and are found to be identical. Both can be converted into''endogenous" cytochrome c. (5) Whether pure cytochrome c contains more than 0.43% iron has been discussed. It seems that no convincing evidence has been presented to show that cytochrome c preparations with iron content higher than 0.43% as obtained by some workers do not contain a small amount of iron-rich impurity.

(一)用陽離子交换劑(synthetic zeolite)直接吸附高等動物心肌抽提液一次,並用3.84M硫酸銨作洗脫劑,即可製得含鐵量0.43%的細胞色素c。因此提供了一個大量製備純細胞色素c的簡單方法。 (二)含鐵量0.43%的細胞色素c,它的550mμ和278mμ光密度的比值,視產品的還原程度而定,其範圍從1.13到1.26,我們所製得的產品,其比值在1.23左右。 (三)我們测量了氧化及還原的細胞色素c(含鐵0.43%)從230mμ到600mμ的吸收光譜,並發現和前人所報告的略有不同。氧化細胞色素c在550mμ的消光係數為0.80×10~4,此值與文獻上的數值相差很多。 (四)我們比較了含鐵量0.43%的細胞色素c和含鐵量0.34%的細胞色素c的一些酶性質,證明他們是相同的;並且兩者都可以變成“內源”細胞色素c。 (五)我們認為現有的實驗證據不足以說明純細胞色素c的含鐵量大於0.43%。

 
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