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   sequencing results 的翻译结果: 查询用时:0.229秒
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sequencing results
相关语句
  测序结果
     DNA sequencing results further confirmed that RT-PCR products were truly Sp32/OY-TES-1 cDNA.
     DNA测序结果证实,RT-PCR产物为Sp32/OY-TES-1基因。
短句来源
     Sequencing results showed that TM full-length cDNA sequence is 1 125 bp,with 5' non-coding region from 1bp to 124 bp,3' non-coding region from 980 bp to 1 125 bp,and the rest composed a ORF which encoding a protein of 284 amino acid residues.
     测序结果表明,TM序列全长1125bp,5'非翻译区为1bp~124bp,3'非翻译区为980bp~1125bp,开放阅读框为125bp~979bp,编码284个氨基酸。
短句来源
     Sequencing results showed that the sequence of 20 samples (20/24,83.3%) were 100% matching the sequence in genebank, while in other 4 samples (4/24,16.7%) a same "C→T" heterozygous point mutation, at 798bp of exon 4 of TSG101 mRNA, was detected.
     测序结果24例组织中20例(83.3%)经序列比对与基因库的TSG101mRNA序列100%一致,另4例(16.7%)均于TSG101基因第4外显子mRNA第798bp处存在“C→T”杂合性点突变。
短句来源
     The sequencing results were aligned with the sequence (Genbank, AY306198, EF012241, L77887, M81327, M92089), indicating that the homology between these two sequences was 99%, indicated that this gene is PLF.
     测序结果与GenBank上登录的序列号为AY306198、EF012241、L77887、M81327、M92089的猪乳铁蛋白进行比对,发现本研究中所克隆的PLF-N基因序列与其它基因报道基因同源性高达99%,确为猪乳铁蛋白基因。
短句来源
     Sequencing results showed that the ORF of CYP305B1V1 was 1464 nucleotides long and encodes 487 amino acids which possesses a common characteristic of Cytochrome P450 proteins.
     测序结果显示,野桑蚕CYP305B1V1 基因的ORF 为1464nt,编码487aa,具有细胞色素P450 蛋白的共同特征。
短句来源
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  序列测定结果
     DNA sequencing results showed that the fragment was 919 bp.
     DNA序列测定结果表明,该片段长度为919bp.
短句来源
     The sequencing results showed that hSDCT 2 cDNA contained 1809 bp open reading frame to encode 602 amino acids.
     序列测定结果显示 ,hSDCT2开放阅读框为180 9bp ,共编码 6 0 2个氨基酸 .
短句来源
     DNA sequencing results showed that bkt was composed of 960 bp and encoded 320 amino acid residues.
     序列测定结果表明,bkt全长960bp编码320个氨基酸。
短句来源
     Sequencing results showed that hSDCT2 cDNA contained 1809 bp full-length open reading frame to encode 602 amino acids.
     序列测定结果显示hSDCT2开放阅读框为1809bp,共编码602个氨基酸。
短句来源
     DNA sequencing results showed that Δ6-desaturase gene was composed of 1 344 base pairs and encoded 448 amino acid residues.
     DNA序列测定结果表明,Δ6-dsa全长1347bp编码448个氨基酸。
短句来源
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  测序分析
     The sequencing results indicated that there were three single nucleotide mutations: A→G at 35 bp,C→T at 248 bp,A→G at 256 bp in the exon2 of the IGF-I gene,but the mutations at 35 bp and 248 bp were missense mutations.
     对第2外显子的多态片段测序分析表明:在35 bp处有碱基A→G的突变,248 bp处有碱基C→T的突变,256 bp处有碱基A→G的突变;
短句来源
     Sequencing results showed that 8 of 16 genes had high homologous to the genes(GBR5,GBR3 and GBR1) known in GenBank,which were involved in biosynthesis of ginsenoside in panax ginseng plant; 8 of 16 genes may be novel genes.
     随机挑取16个cDNA克隆体进行测序分析,结果显示16个克隆体中有8个同GenBank中登录的人参皂苷合成相关基因GBR5,GBR3和GBR1高度同源,8个为新的基因序列.
短句来源
     The sequencing results confirmed that the PCR product was specific for DD3 mRNA.
     PCR扩增产物经测序分析证实为DD3mRNA特异性片段。
短句来源
     The sequencing results showed that there was a mutation (C→G) at 363bp of exon 1 of ESR gene in BB genotype compared to AA genotype.
     对两种纯合子基因型(AA、BB)进行测序分析,结果表明:BB型和AA型相比在外显子1第363位发生1处碱基突变(C→G)。
短句来源
     The HLA gene sequencing results of some special cases further confirmed the specificity of HLA class I PCR SSP typing results.
     HLA基因测序分析进一步肯定了HLA I类PCR SSP分型的特异性。
短句来源
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  “sequencing results”译为未确定词的双语例句
     1. According to the sequencing results of the 5ˊ and 3ˊsegments of cNHX1 gene,4primers ,F01,F02,R01 and R02,were designed adopted to the principle of SOEing method.
     1.根据测定的 cNHX1 基因 5ˊ和 3ˊ片段序列,按照 SOEing 方法的原则,设计了4 条引物,F01、F02、R01 和 R02。
短句来源
     RESULTS:Sequencing results showed that the ND-1scFv- CD gene consisted of 1 269 bp,including ND-1scFv 732 bp and CD 477 bp.
     结果:序列测定表明:ND-1scFv-CD基因全长1269bp,包含了732bp的scFv基因和477bp的CD基因.
短句来源
     Two recombined plasmids of pCB-opn 15 and pCB-opn 39 were obtained,and the sequencing results showed that pCB-opn 15 was inserted in positive orientation and pCB-opn 39 was inserted in inversion.
     把已克隆的草菇开伞相关基因DNA片段正反向插入pCB,获得pCB opn15和pCB opn39,经测序证实,pCB opn15为正向插入,pCB opn39为反向插入。
短句来源
     The sequencing results indicated that in comparison with the reported corresponding sequences (GenBank No.:AY187271) the similarities of nucleotide and putative amino acid sequences of the cloned lipL21 genes from these four leptospiral strains were as high as 99.64%-99.82% and 99.46%-100%,respectively.
     序列分析结果表明,所克隆的4株钩体lipL21基因与已报道的相应序列(GenBankNo.:AY187271)比较,其核苷酸和氨基酸序列相似性分别高达99.64~99.82%和99.46~100%。
短句来源
     The sequencing results showed that CP genes of PRSV-W isolates from Menzi (PRSV-MZ) and Shiping(PRSV-SP) in Yunnan had 873bp,coding for 291 amino acids, but the those from Eshan(PRSV-ES), Banna(PRSV-BN) and Bingchuan(PRSV-BC) were 867bp, coding for 289 amino acids.
     结果表明,PRSV蒙自分离物(PRSV-MZ)和石屏分离物(PRSV-SP)CP基因核苷酸序列全长873bp,编码291个氨基酸,PRSV峨山分离物(PRSV-ES)、版纳分离物(PRSV-BN)和宾川分离物(PRSV-BC)全长867bp,编码289个氨基酸。
短句来源
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  sequencing results
The hybridization and sequencing results thus indicate a closer genomic relationship between BCV and HCV-OC43 than there is between HEV and BCV or HCV-OC43, respectively.
      
Sequencing results indicated that the DNA-A sequence of TYLCV-Sh10 contained 2781 nt that included six ORFs.
      
However, our sequencing results suggested that the LMP2A protein in EBVaGC is functionally similar to that of the B95-8 strain and is not unique to gastric carcinoma, indicating the importance of LMP2A for EBV latency.
      
The sequencing results indicated that the intact enzyme had a blocked N-terminal and there was a 10-amino-acid sequence in the N-terminal of the core protein of Ser-Gly-Thr-Ala-Val-Thr-Cys-Leu-Ala-Asp.
      
SSCP analysisconfirmed the sequencing results and revealed noevidence for the emergence of new viral subpopulationsbefore seroconversion.
      
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Duck hepatitis B virus ( DHBV)in a single DHBV positive serum-number 76 from Lujiang county, China, was replicated in DHBV-DNA negative one day old Beijing ducklings. Viral DNA was purified, digested with EcoR I, ligated to pUC 18, transformed into E. coli JM 105. Using Southern transfer hybridization, we have obtained a clone, pLJ 76, containing 3.0 kb DHBV genome. Restriction mapping of this clone was constructed with 11 restriction enzymes. Compared with DHBV clones of USA and Germany, we found there were...

Duck hepatitis B virus ( DHBV)in a single DHBV positive serum-number 76 from Lujiang county, China, was replicated in DHBV-DNA negative one day old Beijing ducklings. Viral DNA was purified, digested with EcoR I, ligated to pUC 18, transformed into E. coli JM 105. Using Southern transfer hybridization, we have obtained a clone, pLJ 76, containing 3.0 kb DHBV genome. Restriction mapping of this clone was constructed with 11 restriction enzymes. Compared with DHBV clones of USA and Germany, we found there were different digestion sites (Acc I, BamH I, EcoR V and Hind I I ) on the Chinese DHBV genome. Using low-melting point agarose DNA extraction method, we purified specific pLJ 76 DHBV-DNA fragments and subcloned them into Ml3-JMl05 system. Plus and minus strand probes of certain DHBV open reading frame including Pre-S, S, X/C and P were obtained. All of these probes were confirmed by DNA sequencing results.

用鸭乙型肝炎病毒(DHBV)阳性的安徽庐江鸭血清感染DHBV阴性的北京雏鸭,扩增病毒,将提取的DHBV-DNA插入pUC18质粒,转化E.coli JM 105。酶切重组质粒及South-ern转膜杂交结果证实,质粒pLJ76的插入片段为DHBV全基因组。用EcoR Ⅰ等11种限制性内切酶对pLJ76进行酶谱分析,并与美国,西德的已知DHBV基因组比较。定向克隆该株病毒不同基因编码区片段,构建正负单链探针,将斑点杂交和单链电泳检出的M13阳性重组子与已知序列的DHBV基因组作比较,提示获得了该株病毒基因组的S、Pre-S、P和X/C等蛋白编码区的正、负单链克隆株。

Recombinant human neutrophil activating protein-1/interleukin-8 (NAP-1/IL-8) from E.coli lyzate was electroblotted from the SDS-PAGE gel onto the PVDF-type problott membrane and its N-terminal amino acid sequence was determined directly.The efficient expression and processing of the target protein were confirmed by the sequencing result.The recombinant NAP-1/IL-8 was then purified through gel filtration (Bio-Gel P30) and cation exchange (Mono-S) chromatography.The purified protein with a specific chemotactic...

Recombinant human neutrophil activating protein-1/interleukin-8 (NAP-1/IL-8) from E.coli lyzate was electroblotted from the SDS-PAGE gel onto the PVDF-type problott membrane and its N-terminal amino acid sequence was determined directly.The efficient expression and processing of the target protein were confirmed by the sequencing result.The recombinant NAP-1/IL-8 was then purified through gel filtration (Bio-Gel P30) and cation exchange (Mono-S) chromatography.The purified protein with a specific chemotactic activity of 2.8**********x105U/mg,as assayed by the agarose plate method, appeared as a single band on SDS-PAGE gel.Based on the fact that NAP-1/IL-8 was eluted earlier in gel filtration than the 14kD internal standard molecule lysozyme, it was estimated that NAP-1/IL-8 dimers were present in solution.

本文利用SDS-PAGE及蛋白质电泳印迹技术,从带有相应表达质粒的重组大肠杆菌裂解液中,将所表达的重组人嗜中性白细胞活化蛋白-1/白细胞介素-8(NAP-1/IL-8)转移至聚偏二氟乙烯膜上,直接进行N-末端15个氨基酸的序列分析,从而确证该目标蛋白得到高效表达和正确加工。随后采用Bio-Gel P30凝胶过滤层析和Mono-S阳离子交换层析对重组人NAP-1/IL-8进行了分离纯化,纯化产品达到SDS-PAGE纯。利用琼脂糖平板法测定了纯化产品的嗜中性白细胞趋化活性,推算其比活为2.8×10~5U/mg蛋白。又利用SDS-PAGE测出重组NAP-1/IL-8的分子量约为8.5kD,但根据凝胶过滤层析的洗脱时间推定,在溶液中确实存在分子量稍大于14.4kD的NAP-1/IL-8二聚体。

This paper discusses the method for limit analysis of quota coeffecient in multi-project sequencing, develops the limit analysis models of quota coefficient which satisfy maintaing-order or weak-maintaining-order quality in order to realize and establish the influence of the varied coefficients on the sequencing result, using the method, the paper also calculates and analyses the limit values of quota coefficients in project sequencing of a gas field development.

本文详细讨论了多方案排序中指标权重界限分析方法,建立了满足保序性和弱保序性的指标权重界限模型,用以认识和确定各指标权重变化对排序结果的影响。最后,利用该方法对某气田开发方案排序的权重界限值进行了计算分析。

 
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