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japonicum circulating
相关语句
  血吸虫循环
     Optimization the conditions for detection of Schistosoma japonicum circulating antigens by piezoelectric immunosensor
     压电免疫传感器检测日本血吸虫循环抗原的研究
短句来源
     Antigen location and primary application of phagy antibodies against Schistosoma japonicum circulating antigen
     抗日本血吸虫循环抗原单链抗体的抗原定位和初步应用
短句来源
     GENE AND SECONDARY STRUCTURE ANALYSIS OF PHAGY ANTIBODIES AGAINS T SCHISTOSOMA JAPONICUM CIRCULATING ANTIGEN
     抗日本血吸虫循环抗原噬菌体抗体基因结构分析
短句来源
     PURIFICATION OF IMMOBILIZED ANTIBODIES FOR DETECTING SCHISTOSOMA JAPONICUM CIRCULATING ANTIGEN IN PIEZOELECTRIC IMMUNOSENSOR
     检测日本血吸虫循环抗原的压电免疫传感器中抗体的纯化与固定
短句来源
  “japonicum circulating”译为未确定词的双语例句
     DETECTION OF SCHISTOSOMA JAPONICUM CIRCULATING 31/32kDa ANTIGEN-ANTIBODY COMPLEX AND ITS VALUE IN DIAGNOSIS FOR THE DISEASE
     日本血吸虫31/32kDa循环免疫复合物的检测及其在疾病诊断中的作用
短句来源
     DETECTING SCHISTOSOMA JAPONICUM CIRCULATING ANTIGEN IN BOVINE SERA BY McAb-Dot-ELISA
     McAb-Dot-ELISA检测牛日本血吸虫病血清循环抗原
短句来源
     Detection of Schistosoma japonicum circulating 31/32kDa antigen antibody complex is useful to increase the detective rate in schistosomiasis.
     检测血吸虫循环免疫复合物(CIC)有助于提高疾病的检出率。
短句来源
     Methods McAb SSJ14 was labeled with HRP and applied to detect the Schistosoma japonicum circulating antigen in bovine sera by McAb Dot ELISA.
     方法 用辣根过氧化物酶标记日本血吸虫单抗 SSJ14 ; 应用 Mc Ab- Dot- EL ISA检测现场和实验室感染牛日本血吸虫血清循环抗原。
短句来源
  相似匹配句对
     japonicum,T.
     japonicum,T.
短句来源
     japonicum, X.
     japonicum ,X .
短句来源
     BIOCHEMICAL DEETECTION OF THE CIRCULATING ANTIGEN OF SCHISTOSOMA JAPONICUM
     日本血吸虫循环抗原的生化检测
短句来源
     Preliminary study on quantitative determination of circulating Antigens of Schistosoma japonicum
     量化检测日本血吸虫循环抗原的初步研究
短句来源
     On Circulating Economy
     论循环经济
短句来源
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Detection of Schistosoma japonicum circulating 31/32kDa antigen antibody complex is useful to increase the detective rate in schistosomiasis. A capture ELISA based on a mono clonal antibody H226 and goat anti-human IgG (FC) conjugated with alkaline phosphatase was used for detection of circulating 31/32 kDa antigen-antibody complex in the sera from patients infected with S. japonicum. The total detective rate was 92. 2%. The positive rate in the patients whose EPG<100 was 90. 0% and 95. 5%...

Detection of Schistosoma japonicum circulating 31/32kDa antigen antibody complex is useful to increase the detective rate in schistosomiasis. A capture ELISA based on a mono clonal antibody H226 and goat anti-human IgG (FC) conjugated with alkaline phosphatase was used for detection of circulating 31/32 kDa antigen-antibody complex in the sera from patients infected with S. japonicum. The total detective rate was 92. 2%. The positive rate in the patients whose EPG<100 was 90. 0% and 95. 5% in the patients whose EPGtr100,there was no significant difference between these two groups. Since goat anti-human IgG (FC) can specificly bind with human IgG Fc fragment which existed in the antigen-antibody complex,therefore,this assay can be used as a more specific and sensitive method,which is easier for detection of circulating antigen-antibody complex in the patients.

检测血吸虫循环免疫复合物(CIC)有助于提高疾病的检出率。利用抗血吸虫单克隆抗体H226和碱性磷酸酶标记的羊抗人IgG(Fc)作捕捉ELISA,检测日本血吸虫病患者血清中CIC,其阳性检出率为92.2%,其中,EPG<100的病人阳性检出率为90.0%,EPG>100的病人阳性检出率为95.5%,两者无显著性差异。由于羊抗人IgGFc段能与CIC中人IgG的Fc段特异性结合,因此不仅省去了常规分离CIC的步骤,而且有助于提高试验的敏感性和特异性。

Objectives To investigate the nature of the common epitopes of Schistosoma japonicum (S. japonicum) circulating anodic (CAA) and circulating cathodic antigen (CCA) and to try to obtain sufficient purified material to set up a standard series for quantitative determinations.Methods Isolation of the two worm fractions from a trichloroacetic acid (TCA) soluble preparation of S. japonicum adult worm antigen (AWAj-TCA) via Mono-Q anion exchange chromatography was performed and...

Objectives To investigate the nature of the common epitopes of Schistosoma japonicum (S. japonicum) circulating anodic (CAA) and circulating cathodic antigen (CCA) and to try to obtain sufficient purified material to set up a standard series for quantitative determinations.Methods Isolation of the two worm fractions from a trichloroacetic acid (TCA) soluble preparation of S. japonicum adult worm antigen (AWAj-TCA) via Mono-Q anion exchange chromatography was performed and analysis of specific reactivity of the eluted fractions was done by antigen-capture Enzyme Linked Immuno Sorbent Assay (ELISA) specific for CAA or CCA with reference to affinity purified preparations of S. mansoni CAA and CCA. Results When an ionic strength gradient was used, CCA was eluted in two major peaks, an unbound fraction CCA-1, and a major bound fraction, CCA-2. Two additional minor peaks, CCA-3 and CCA-4, were eluted at higher ionic strengths. CAA was only detected in the bound fraction, partly overlapping with CCA-3. In the CCA-1 and CCA-2 fractions, reactivity was only found in the antigen-capture ELISA using anti-CCA McAbs both for capture and detection. The CAA fraction was predominantly found to be positive in the antigen-capture ELISA using anti-CAA McAbs both for capture and detection. However, in ELISA using combined anti-CCA and anti-CAA McAbs for capture and detection, this fraction showed some reactivity. Conclusion The two CCA fractions contain molecules which bear at least two CCA-epitopes; the CAA fraction contains molecules which contain at least two CAA-epitopes, and one CCA-epitope.

目的 为探讨血吸虫肠相关循环抗原CAA与CCA诊断靶微粒上表位特异性的差别 ,并试探获取其纯化制品用于定量检测的标准系列。方法 对两组分进行了亲和层析及阴离子交换剂高压液相纯化分离 ,并应用单抗检测系统进行同相和异相交互测试。结果 Mono QHPLC梯度洗脱分离AWAj-TCA可溶组分 ,可获得一个带阳离子活性的非结合CCA - 1峰及 3个大小不等的 ,带阳离子活性的CCA - 2 ,CCA - 3,CCA - 4非结合洗脱峰 ,以及一个带阴离子活性CAA - 1洗脱峰。CAA活性峰在峰谱上与CCA - 3有部分重叠。与单抗亲和层析纯品的活性对比测定显示CCA - 1与CCA - 2为该组分的主要构成 ,但CCA - 2及CAA - 1在本实验条件下均有微量的相互杂染。同相和异相双位点ELISA的 4种组合交互检测 ,展示CCA只能在捕获与检测抗体同为抗CCA单抗的一种组合中被检示 ,而另 3种组合都只能测出CAA组分。结论 血吸虫肠相关CCA组分为一兼含两性电荷的分子混合体 ;而CAA分子上具有一个可被抗CCA单抗识别的活性表位位点 ,从而可能影响纯化分离和特异检测。

A new liquid piezoelectric immunosensor(PEIS) was developed to detect Schistosoma japonicum circulating antigens(SjCAg) in infected rabbit sera by immobilizing the purified IgG antibodies from infected rabbit sera(InRS) or immunized rabbit sera(ImRS) onto the golden electrode of piezoelectric quartz crystal through SPA The effect of the different kind antiserum,including InRS and ImRS and the purity of IgG,including crude sera,partially purified and fully purified IgG,were compared in the efficiency...

A new liquid piezoelectric immunosensor(PEIS) was developed to detect Schistosoma japonicum circulating antigens(SjCAg) in infected rabbit sera by immobilizing the purified IgG antibodies from infected rabbit sera(InRS) or immunized rabbit sera(ImRS) onto the golden electrode of piezoelectric quartz crystal through SPA The effect of the different kind antiserum,including InRS and ImRS and the purity of IgG,including crude sera,partially purified and fully purified IgG,were compared in the efficiency of immobilization and the detection of SjCAg by PEIS It was demonstrated that the immunized rabbit sera was better than the infected rabbit sera,and the fully purified IgG was the best among samples with different IgG purity.The concentration of SPA for immobilizing antibodies,the reaction time,the optimum dilution of detected sera and the regeneration of crystal were also observed Rabbits'sera with various degree of infection were examined It was found that frequency change was directly proportional to the degree of infection in a certain range

通过葡萄球菌蛋白A(SPA)将从感染兔血清 (InRS)或免疫兔血清 (ImRS)中纯化的IgG抗体固定于镀金的石英晶片上 ,制成一种新型的液相压电免疫传感器 ,用于检测感染兔血清中的日本血吸虫循环抗原 (SjCAg)。分别比较了提纯前血清、部分纯化抗体和完全纯化抗体 ,以及感染和免疫兔血清纯化IgG的固定效率和检测效果。结果表明 ,以完全纯化抗体的效果最好 ,免疫兔血清纯化IgG优于感染兔血清纯化IgG。同时 ,还研究了SPA的固定浓度、检测时间、血清最适稀释度及晶体的再生性等。用该法检测了不同感染度的兔血清 ,发现频移值变化在一定范围内与感染度成正比

 
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