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optimal hormone combinations
相关语句
  最佳激素组合
     Method: Out the optimal hormone combinations for callus induction,adventitious bud initiation,adventitious bud multiplication and rooting were found out by using MS medium with the macroelements at half strength as basal medium and studying the effects of BA,NAA and IBA on the processes.
     方法 :以大量元素减半的MS培养基为基本培养基 ,通过研究BA ,NAA和IBA对愈伤组织诱导、不定芽分化、不定芽增殖和生根的影响找出相应的最佳激素组合
短句来源
  “optimal hormone combinations”译为未确定词的双语例句
     This paper is about somatic embryogenesis and propagation from cotyledons of immature and mature zygotic embryos in Manchurian ash. It was observed that different optimal hormone combinations was necessary for induction of somatic embryogenesis of various source trees, also, the frequency of somatic embryogenesis are different, such as source tree 3, NAA 2 mg/L and BA 0.5 mg/L, 54%;
     以同一地点不同母树来源的水曲柳未成熟合子胚子叶为外植体,进行体细胞胚胎发生研究,研究结果表明,不同的母树来源的子叶体细胞胚发生诱导需要不同的最适激素组合,其诱导频率也不同,分别为:母树3,NAA2mg/L+BA0.5mg/L,其诱导频率为54%;
短句来源
     Method: MS medium was used as basal medium and BA and NAA were supplemented to find out the optimal hormone combinations for adventitious buds initiation, adventitious bud multiplication and rooting.
     方法:以MS培养基为基本培养基,通过研究植物生长物质BA和NAA对丛生芽的分化、丛生芽的增殖和生根的影响找出相应的最佳生长物质组合。
短句来源
  相似匹配句对
     hormone
     激素
短句来源
     polypeptide hormone;
     多肽激素;
短句来源
     Optimal temperature for D.
     各种光强下的暗呼吸速率均随温度升高而增大。
短句来源
     It is the optimal pattern.
     这个结果如同自然界生态平衡一样,是相对最优的格局。
短句来源
     Influence of continuous combined hormone replacement therapy on endometrium-search of optimal regimen
     连续联合并用性激素补充疗法对子宫内膜的影响——最佳方案的探索
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Mature shoots from grafted adult trees of longan were sterilized and cut into 0.5 cm shoot tips which were used as explants and inoculated onto basal media such as MS, N 6, 1/2MS and White supplemented with various mass concentrations of BA, IAA, and GA 3, in order to induce regeneration. The results showed that MS and N 6 were suitable for inducing buds from longan shoot tips and the optimal hormone combination was 0.3 mg·L -1 BA + 0.2 mg·L -1 IAA + 0.5 mg·L -1 GA 3. In the...

Mature shoots from grafted adult trees of longan were sterilized and cut into 0.5 cm shoot tips which were used as explants and inoculated onto basal media such as MS, N 6, 1/2MS and White supplemented with various mass concentrations of BA, IAA, and GA 3, in order to induce regeneration. The results showed that MS and N 6 were suitable for inducing buds from longan shoot tips and the optimal hormone combination was 0.3 mg·L -1 BA + 0.2 mg·L -1 IAA + 0.5 mg·L -1 GA 3. In the subsequent subculture, the induced buds were transferred to the medium MS + 0.3 mg·L -1 BA + 0.2 mg· -1 IAA, and 1-3 new buds were regenerated from each one in 30-50 days, and then developed into plantlets, which rooted on the medium 1/2MS + 0.1 mg·L -1 BA + 0.5 mg·L -1 IBA for 3-4 weeks and grew into normal plants.

选用嫁接成年树的龙眼成熟枝梢作为外植体 ,经常规消毒后 ,接种于附加不同质量浓度的激素 BA、IAA和 GA3 的 MS、N6、 1/ 2 MS、White等基本培养基上 ,诱导茎尖萌芽 .结果表明 ,MS、N6培养基适合于龙眼茎尖芽的诱导 ,最佳的激素组合为 0 .3mg·L-1BA+0 .2 mg· L-1IAA+0 .5mg· L-1GA3 .将诱导的芽转移到 MS+0 .3mg· L-1BA+0 .2 mg· L-1IAA培养基上 ,30 - 50 d内可增殖 1- 3个幼芽 ,并正常抽枝 ,形成小苗 .将小苗转入 1/ 2 MS+0 .1mg·L-1BA+0 .5mg· L-1IBA培养基 ,3- 4周后可生根 ,长成正常植株

The tender bud and shoot segment of emblic were in vitro cultured on the MS medium. Results indicated that the addition of BA had a marked promoting effect on bud induction. The optimal hormone combination was found to be BA with NAA. For the bud explant, the optimal medium dosage for bud induction was MS added with 0.5 mg/L of BA and 0.1 mg/L of NAA, and the differentiating rate was above 90%. And for the shoot explant, the concentration of BA required for bud induction was higher, and the optimal...

The tender bud and shoot segment of emblic were in vitro cultured on the MS medium. Results indicated that the addition of BA had a marked promoting effect on bud induction. The optimal hormone combination was found to be BA with NAA. For the bud explant, the optimal medium dosage for bud induction was MS added with 0.5 mg/L of BA and 0.1 mg/L of NAA, and the differentiating rate was above 90%. And for the shoot explant, the concentration of BA required for bud induction was higher, and the optimal dosage was MS added with 2.0 mg/L of BA and 0.1 mg/L of NAA, the differentiating rate was 56.7%. For bud multiplication, the medium used was MS added with 0.1 mg/L of BA, 0.1 mg/L of NAA, and 50 mg/L of NaH 2PO 4. For root induction, the medium used was 1/2 MS added with 0.25 mg/L of NAA and 0.25 mg/L of IBA, which generated a rooting rate of 30%.

本试验以余甘子嫩芽和嫩枝茎段为外植体 ,在 MS培养基中进行离体繁殖和培养试验。结果表明 ,添加细胞分裂素 BA对丛生芽诱导有明显促进作用 ,激素组合以 BA与 NAA为最好 ,嫩芽外植体诱导丛生芽的最适培养基为 MS+BA0 .5 mg/ L+NAA 0 .1mg/ L,分化率达 90 %以上 ;嫩枝茎段旅导丛生芽所需的 BA浓度相对高些 ,最适培养基为 MS+BA 2 .0 mg/ L +NAA 0 .1mg/ L,分化率达 5 6 .7% ,丛生芽增殖生长培养基为 MS+BA 0 .1mg/ L +NAA 0 .1mg/ L +Na H2 PO45 0 mg/ L。生根培养基为 1/ 2MS+NAA 0 .2 5 mg/ L+IBA 0 .2 5 mg/ L ,生根率在 30 %左右

Four pepper (Capsicum annuum L.) varieties were selected as experimental materials. Their cotyledons and hypocotyls were used as explants so that an experiment could be conducted on the induction and differentiation, the growth, and the rooting of adventitious buds. As different hormones with various massconcentrations were added into the media used for each stage of tissue culture, the growth of explants in each medium was investigated, and the optimal hormone combination was sifted out through...

Four pepper (Capsicum annuum L.) varieties were selected as experimental materials. Their cotyledons and hypocotyls were used as explants so that an experiment could be conducted on the induction and differentiation, the growth, and the rooting of adventitious buds. As different hormones with various massconcentrations were added into the media used for each stage of tissue culture, the growth of explants in each medium was investigated, and the optimal hormone combination was sifted out through the investigation. Thus a system for in vitro high efficient regeneration of pepper was established. Based on the above system, the Kanamycin(Kan) with different massconcentrations was respectively added into the media, which were used for inducing the callus of cotyledons and hypocotyls and for differentiating adventitious buds during each stage. After that, the effects of Kan on the growth of explants were observed so as to determine its minimum lethal massconcentration, which was to be used for studying genetic transformation of pepper. The experimental results showed that the optimal medium for the stage of bud differentiation was MS with 3.0 mg·L-1 of 6-BA plus 0.1 mg·L-1 of IAA, for the stage of adventitious buds' growth was MS with 2 mg·L-1 of GA3 plus 0.5 mg·L-1 of 6-BA, and for inducing rootgrowth was MS with 1.0 mg·L-1 of IAA. In addition, for the callus formation of hypocotyls, the minimum lethal massconcentration of Kan was 80 mg·L-1, and for the differentiation of adventitious buds, it was 60 mg·L-1. So it was thought that this study could provide the prerequisite for genetic transformation of pepper.

选用辣椒(CapsicumannuumL.)4个品种的子叶以及下胚轴为外植体进行不定芽的诱导分化、生长及生根成苗试验.在组织培养的各个阶段的培养基中,附加不同种类及不同质量浓度的激素组合,观察测量外植体在各种培养基中的生长情况,从中筛选出最优的激素配比培养基,从而建立了辣椒的高效离体植株再生体系.在此基础上,将不同质量浓度梯度的卡那霉素(Kan)分别加入子叶、下胚轴愈伤组织诱导及不定芽分化阶段的培养基中,观测Kan对外植体生长的影响,以确定各阶段的最低致死质量浓度,用于辣椒的遗传转化研究.试验结果表明最佳的辣椒离体再生培养基为:芽分化阶段,MS+6-BA3.0mg·L-1+IAA0.1mg·L-1;不定芽伸长阶段,MS+GA32mg·L-1+6-BA0.5mg·L-1;诱导生根,MS+IAA1.0mg·L-1.合适的Kan质量浓度为:下胚轴出愈为80mg·L-1;子叶分化出不定芽为60mg·L-1.

 
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