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primary anti
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  一抗
     Instead of primary anti, we use normal primary anti animal blood serum as the negative contrast, the antibody is provided by Beijing zhongshan biotechnology company.
     以正常一抗动物血清代替一抗做阴性对照 ,抗体由北京中山生物技术公司提供。
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  “primary anti”译为未确定词的双语例句
     CLINICAL USE OF FK506 AS PRIMARY ANTI REJECTION AGENT IN RENAL ALLOGRAFT RECIPIENTS
     肾移植术后抗排斥药FK506的临床应用
短句来源
     Fusion protein could specifically react with the primary anti ovarian carcinoma monoclonal antibody(COC166 9) and rat anti mouse GM CSF monoclonal antibody, respectively, and fusion proteins could also stimulate murine GM CSF dependent cell line NFS 60 cells to proliferate.
     表达的融合蛋白分别能与 COC16 6 - 9单抗和大鼠抗小鼠 GM- CSF单抗特异结合 ,并能刺激 m GM- CSF依赖株 NFS- 6 0细胞增殖。
短句来源
     The expressed proteins after purification could specifically react with the primary anti ovarian carcinoma monoclonal antibody (COC166 9). Inhibition rate could reach 67% by inhibition ELISA.
     纯化后的表达蛋白6B11scFv能与HRPCOC1669卵巢癌单抗反应并与卵巢癌抗原共同竞争结合卵巢癌单抗COC1669,抑制率达67%,Westernblot显示其相对分子质量为28×104,证明纯化后的表达蛋白质,能模拟卵巢癌抗原与卵巢癌单抗特异结合。
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     CONCLUSION FK506 used as a primary anti rejection agent is effective and safe in renal graft recipients.
     结论 :FK5 0 6是肾移植术后有确切疗效的基础抗排斥药 ,与MMF、皮质醇合用能有效地预防急性排斥的发生 ,并可控制慢性排斥的进展。
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     CA11and contro l vectors were steadily transfected into gastric cell line 7901respectively by lipofectamin. Cellular immunohistochemistry with primary anti Myc-tag antibody and antibodies against eight known suppressor genes and oncogenes was carried out on glass slides after transfection with CA11and control v ectors.
     CA11与空载稳定转染胃癌细胞株7901,细胞爬片后行抗Myc标签及8种抑癌基因、癌基因的细胞免疫组织化学分析。
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  相似匹配句对
     Anti-H.
     方法:将H.
短句来源
     Anti-S.
     体外模拟试验发现抗S.
短句来源
     Primary Exploration on Function of anti-senility of Huanghua
     黄花参抗衰老作用的初步探讨
短句来源
     The primary principle of anti-vibration on vehicle is introduced.
     给出了汽车隔振基本原理.
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     Primary Aldosteronism
     原发性醛固酮增多症
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  primary anti
Its primary anti-inflammatory mechanism of action is through a selective effect on cyclo-oxygenase-2 (COX-2).
      
Single doses of FCE 23762 and Dx, given concomitant or after the antigen, suppressed at the same degree and dose-dependently the primary anti-SRBC antibody response.
      
The view that inhibition of prostaglandin biosynthesis is the primary anti-inflammatory mechanism of NSAI's in rheumatoid arthritis is discussed in terms of these findings.
      
Herein we present a patient with primary anti-phospholipid syndrome who developed CAPS manifested by hepatic, renal and splenic artery thromboses, as well as cerebral venous thrombosis.
      
Primary anti-phospholipid syndrome (APS) is perceived to be an uncommon disorder, infrequently recognized as a cause of renal disease in childhood.
      
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Objective:To clone and express 6Bll ovarian ctqrcinoma anti-idioiypic antihody(Ab2β)singlechain Fv(ScFv)genes.To analyze the nucleotide sequence of ScFv.Methods:Using RT-pCR,the vari-able region genes of heavy llnd light chains(VH and VL)of 6B11 were amplified and assembled with aflexible linker sequence to form ScFv genes,which were then cloneed into,bacterial expresxion vectors toproduce proteinx,Nucleotide sequences of VH and VL were analyzed.Results:Expressed proteins fromsome clones could interact specifically...

Objective:To clone and express 6Bll ovarian ctqrcinoma anti-idioiypic antihody(Ab2β)singlechain Fv(ScFv)genes.To analyze the nucleotide sequence of ScFv.Methods:Using RT-pCR,the vari-able region genes of heavy llnd light chains(VH and VL)of 6B11 were amplified and assembled with aflexible linker sequence to form ScFv genes,which were then cloneed into,bacterial expresxion vectors toproduce proteinx,Nucleotide sequences of VH and VL were analyzed.Results:Expressed proteins fromsome clones could interact specifically with the primary anti-Ovarian carcinoma monoclonal antibody(COC166-9).The analysis of nucleotide sequence indicated that 6B11 VH and VL genes belonged to themouse Ig heavy chain subgroup I and light chain subgrnup Ⅱ,respectively.Conclusion:6B11 Ab2βSeFvgenes were successfully cloned and expressed,whch may play an imporlant role in genetically manipu-lating The murine Ah2 and make it close to clinical application for immunothcrapy of ovarian cancer.

目的:克隆和表达6B11卵巢癌抗独特型抗体单链可变区基因,并进行序列分析。方法:采用逆转录PCR和基因重组技术,扩增并构建6B11鼠抗独特型抗体单链可变区基因,重组至噬菌体表面表达载体,在大肠杆菌表达,并利用双脱氧末端终止法进行核苷酸序列分析。结果:阳性克隆表达产物与卵巢癌单克隆抗体COC166-9特异结合;DNA序列分析表明重链和轻链可变区分属小鼠免疫球蛋白重链第I亚群和轻链第Ⅱ亚群。结论:自6B11小鼠杂交瘤细胞成功地克隆表达了该抗独特型单链抗体基因,为进一步人源化改造,推进卵巢癌的免疫治疗奠定了良好基础。

Objective: To study the renaturation, purification and binding activity of 6B11 ovarian carcinoma anti idiotypic antibody scFv expressed as inclusion bodies in E. coli . Methods: Inclusion bodies collected after the breakage of bacteria through sonication were subjected to repeatedly washing. Inclusion bodies solubilized in the presence of 8 mol·L -1 urea were diluted with renaturation solutions so that folding process could be initiated. The renaturation conditions were optimized including time, temperature...

Objective: To study the renaturation, purification and binding activity of 6B11 ovarian carcinoma anti idiotypic antibody scFv expressed as inclusion bodies in E. coli . Methods: Inclusion bodies collected after the breakage of bacteria through sonication were subjected to repeatedly washing. Inclusion bodies solubilized in the presence of 8 mol·L -1 urea were diluted with renaturation solutions so that folding process could be initiated. The renaturation conditions were optimized including time, temperature and oxidized glutathione (GSSG) and reduced glutathione (GSH) concentration, respectively. 6B11scFv renatured proteins were purified on DEAE Sepharose Fast Flow ion exchange column with salt gradient elution. The purity was examined by SDS PAGE. The binding activities were determined by Western blot, ELISA and inhibition ELISA, separately. Results: By means of DEAE Sepharose Fast Flow, 6B11scFv proteins were obtained with a purity of over 95%. The optimum conditions were 5 mmol·L -1 and 0.5 mmol·L -1 for the concentrations of GSSG and GSH, respectively, and renatured time more than 48 hours at 10 ℃. The expressed proteins after purification could specifically react with the primary anti ovarian carcinoma monoclonal antibody (COC166 9). Inhibition rate could reach 67% by inhibition ELISA. Western blot showed that tested protein could react with COC166 9 that appeared at 2.8×10 4, i.e. the purified protein could mimic

目的:探讨以细菌胞浆内包涵体形式表达的6B11卵巢癌抗独特型单链抗体(6B11scFv)的复性、纯化及其活性。方法:重组质粒pL6B11scFv转化大肠杆菌pop2136,温度诱导,以包涵体形式获高效表达。超声破碎细菌细胞得包涵体,用8mol·L-1尿素溶解包涵体后直接稀释复性,探讨复性条件并采用DEAESepharoseFastFlow离子交换层析纯化复性蛋白,SDSPAGE分析蛋白纯度,Westernblot、ELISA及竞争抑制ELISA测活性。结果:复性蛋白质经纯化,纯度达95%以上。当氧化型谷胱甘肽(GSSG)浓度为5mmol·L-1,还原型谷胱甘肽(GSH)浓度为0.5mmol·L-1,10℃复性48h以上时,复性率较高。纯化后的表达蛋白6B11scFv能与HRPCOC1669卵巢癌单抗反应并与卵巢癌抗原共同竞争结合卵巢癌单抗COC1669,抑制率达67%,Westernblot显示其相对分子质量为28×104,证明纯化后的表达蛋白质,能模拟卵巢癌抗原与卵巢癌单抗特异结合。结论:以包涵体表达的6B11scFv,经溶解复性纯化后,纯度高,活性好。

Objective To construct expression vector which expresses fusion protein of antiidiotypic single chain antibody 6B11ScFv and murine GM CSF(6B11mGM) for observing its possible immune reactions in vivo. Methods Using DNA recombinant techniques, the murine GM CSF cDNA gene was recombined to 6B11ScFv and they were cloned into expression vector pET 30a(+) to produce insoluble protein. Inclusion bodies collected after the breakage of bacteria through sonication were subjected to repeatedly washing. Inclusion...

Objective To construct expression vector which expresses fusion protein of antiidiotypic single chain antibody 6B11ScFv and murine GM CSF(6B11mGM) for observing its possible immune reactions in vivo. Methods Using DNA recombinant techniques, the murine GM CSF cDNA gene was recombined to 6B11ScFv and they were cloned into expression vector pET 30a(+) to produce insoluble protein. Inclusion bodies collected after the breakage of bacteria through sonication were subjected to repeatedly washing. Inclusion bodies solubilized in the resence of 8 mol·L -1 urea were diluted with renaturation solutions so that folding process could be initiated. The purity was examined by SDS PAGE, ELISA and cell proliferation assay were used to determine the activities of fusion protein. Results 6B11mGM fusion proteins were obtained with a purity of over 90%. The optimum conditions were 1mmol·L -1 and 5 mmol·L -1 for the concentrations of GSSG and GSH, respectively, with 48 hours at 10℃ as renatured time. Fusion protein could specifically react with the primary anti ovarian carcinoma monoclonal antibody(COC166 9) and rat anti mouse GM CSF monoclonal antibody, respectively, and fusion proteins could also stimulate murine GM CSF dependent cell line NFS 60 cells to proliferate. Conclusion Fusion proteins 6B11mGM expressed as inclusion bodies can keep the activity of both the proteins and provid foundation to research the immunoreactions in vivo.

目的 构建卵巢癌抗独特型单链抗体 6 B11Sc Fv和鼠 GM- CSF融合蛋白 (6 B11m GM) ,以观察其在动物体内诱导的特异性免疫反应 ,为卵巢癌抗独特型疫苗应用于临床提供依据。 方法 用 DNA重组技术 ,将m GM- CSF连于单链抗体 6 B11Sc Fv羧基末端 ,构建重组质粒 p ET30 - 6 B11m GM,转化大肠杆菌 BL2 1(DE3) ,IPTG诱导 ,以包涵体形式获高效表达 ,超声破碎细菌细胞获得包涵体 ,用 8mol.L- 1尿素溶解包涵体后直接稀释复性 ,SDS- PAGE分析蛋白纯度 ,EL ISA分析技术和细胞增殖实验测定融合蛋白的抗体和细胞因子活性。 结果 复性蛋白纯度达 90 %以上。采用氧化型谷胱甘肽 (GSSG)浓度为 1mm ol.L- 1 ,还原型谷胱甘肽 (GSH)浓度为 5 m mol.L- 1 ,10℃复性 48h,复性率达 36 %。表达的融合蛋白分别能与 COC16 6 - 9单抗和大鼠抗小鼠 GM- CSF单抗特异结合 ,并能刺激 m GM- CSF依赖株 NFS- 6 0细胞增殖。 结论 以包涵体表达的融合蛋白 6 B11m GM保留了...

目的 构建卵巢癌抗独特型单链抗体 6 B11Sc Fv和鼠 GM- CSF融合蛋白 (6 B11m GM) ,以观察其在动物体内诱导的特异性免疫反应 ,为卵巢癌抗独特型疫苗应用于临床提供依据。 方法 用 DNA重组技术 ,将m GM- CSF连于单链抗体 6 B11Sc Fv羧基末端 ,构建重组质粒 p ET30 - 6 B11m GM,转化大肠杆菌 BL2 1(DE3) ,IPTG诱导 ,以包涵体形式获高效表达 ,超声破碎细菌细胞获得包涵体 ,用 8mol.L- 1尿素溶解包涵体后直接稀释复性 ,SDS- PAGE分析蛋白纯度 ,EL ISA分析技术和细胞增殖实验测定融合蛋白的抗体和细胞因子活性。 结果 复性蛋白纯度达 90 %以上。采用氧化型谷胱甘肽 (GSSG)浓度为 1mm ol.L- 1 ,还原型谷胱甘肽 (GSH)浓度为 5 m mol.L- 1 ,10℃复性 48h,复性率达 36 %。表达的融合蛋白分别能与 COC16 6 - 9单抗和大鼠抗小鼠 GM- CSF单抗特异结合 ,并能刺激 m GM- CSF依赖株 NFS- 6 0细胞增殖。 结论 以包涵体表达的融合蛋白 6 B11m GM保留了两种蛋白的活性 ,为研究融合蛋白在体内的免疫功能提供了基础

 
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