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   culture plate 的翻译结果: 查询用时:0.212秒
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culture plate
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  培养板
     Then they were subcultured in 24-well culture plate with the concentration of 1×106,5×105, 1×105, 5×104, 1×104, 5×103 cells/well ,respectively.
     并采用本实验室自行研制的角膜内皮细胞培养体系,在24孔培养板中分别以1×106,5×105,1×105,5×104,1×104,5×103细胞/孔浓度传代培养细胞,观察细胞的生长情况。
短句来源
     Using the 12 holes plastic culture plate, the 0.5 mL cells at the concentration of 2×105 each per mL that had macrophage character in every hole, and divided randomly into 4 groups.
     采用24孔塑料培养板,每孔加入有巨噬细胞特性、浓度为2×105个/mL细胞0.5mL,随机分成4组。
短句来源
     Methods Experiment against cecariae: The solutions of niclosamide in the concentrations of 10, 5, 1, 0. 5, 0. 1, 0. 05 mg/L and 0. 01 mg/L were made of 25% suspension concentrate of niclosamide. The 0. 3 ml solution for each concentration group was transfused into a hole of the 48-hole culture plate, 20 - 50 cercariae were put into the solution, and the survival situation of cercariae was observed under a dissecting microscope.
     方法杀蚴实验:用25%氯硝柳胺悬浮剂配制氯硝柳胺基质浓度分别为10、5、1、0.5、0.1、0.05、0.01 mg/L溶液,每个浓度组分别取药液0.3 ml置于48孔培养板中,后加入活尾蚴20-50条,解剖镜下观察尾蚴的存活。
短句来源
     Methods(With) same number of total cell in 24-well tissue culture plate,cell activity was evaluated on the 5th day after use of 1,25-(OH)_2D_3 and with same number of MSC in 96-well tissue culture plate,alkaline phosphatase(ALP) activity and osteocalein(OCN) production were detected on the 3rd,5th,and 12th day after 1,25-(OH)_2D_3 application.
     方法以相同总细胞数接种24孔细胞培养板,1,25-(OH)2D3作用5d后,MTT比色法测定细胞活性。 以相同RM-SO细胞数接种96孔细胞培养板,分别于1,25-(OH)2D3作用3、5、12d时,测定细胞ALP活性、OCN含量。
短句来源
     In the first experiment, hepatic cells were cultured with 10% blood serum media supplemented with 0, 15.6, 31.2, 46.8, 62.4, 78, 93.6umol/L Cu in 24 aperture culture plate .
     实验一利用24孔细胞培养板培养细胞,细胞培养液中添加10%小牛血清,同时分别添加0、15.6、31.2、46.8、62.4、78、93.6μmol/LCu,观察细胞形态,测定细胞培养液中CuZn-SOD酶活性。
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  培养皿
     Results:In 35 mm cell culture plate,2 μg DNA with 4μL liposome resulted in the highest transfection efficiency and the morphology of CNE-2Z cell can not saw by using microscopy after 8 h transfection.
     结果 :在 35 mm培养皿中 ,2 μ g DNA与 4μ L脂质体转染 CNE- 2 Z细胞 8h,转染效率最高 ,且在显微镜下观察不到细胞的形态变化。
短句来源
     ③ Cells were seeded in 20 35-mm plates at 1×107 /L. Three hours after inoculation, the culture plate of cells were randomly divided into 5 groups: Normoxic control group, slight hypoxic group, medium slight hypoxic group, CoCl2 group and slight hypoxia + CoCl2 group with 4 plates in each group.
     ③以1×107L-1接种于20个35mm培养皿中,接种细胞3h后,将培养皿随机数字表法分为5组:常氧对照组、轻度低氧组、中度低氧组、CoCl2组、轻度低氧+CoCl2组,4皿/组。
短句来源
     AECⅡ was plated into the wells of six - well cluster dishes at a density of 5×105/mL. LF was plated into the Millicell culture plate inserts at a density of 1×106 /mL.
     AECⅡ以5×105/mL的密度接种到6孔板中,LF以1×106/mL的密度接种到PCF插入式Millicell培养皿中,以6孔板中不放入套皿单独培养的AECⅡ为对照。
短句来源
     Methods: Different doses of garlic oil were added to the HP 8-1, NCTC11639, J99 culture plate, and the plate with the above three bacteria were then incubated at 37℃ for 72 hours to observe the minimum inhibitory concentration(MIC).
     方法 :将不同剂量的大蒜油分别加入含有HP 8 1、NCTC116 39、J99菌株的培养皿中 ,37℃培养 72h后观察最低抑菌浓度 (MIC)。
短句来源
     Methods Thirty health male rabbits were divided into two groups randomly,A and B. Autologous endothelial cells(group A)were harvested with 0.2% collagenase and cultivated in 60mm plastic culture plate to the second generation.
     方法取健康成年雄性大白兔30只,随机分为A、B两组。 取A组一侧颈静脉,在体外以0.2%的胶原酶消化获取其内皮细胞,并在60mm塑料培养皿中传代培养至第二代;
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  文化板块
     AN ANNOTATION ON THE NATURE—CULTURE PLATE OF NORTHWEST YUNNAN
     “滇西北自然—文化板块”诠释
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  “culture plate”译为未确定词的双语例句
     100 μL cell suspension was inoculated in each well of 96-well culture plate, and 5 mL suspension was inoculated in 25 cm2 culture bottle covered with collagen and cultured for 24 hours, then with additional 50 μmol/L and 100 μmol/L olanzapine, respectively, for 24 hours, and β-amyloid 25-35 of different concentrations (0.01 μmol/L, 2 μmol/L and 20 μmol/L) for 24 hours.
     在96孔板每孔中接种100μL细胞悬液,在胶原被覆的25cm2培养瓶中接种5mL细胞悬液,培养24h后,分别加50μmol/L、100μmol/L奥氮平培养24h,再加不同浓度的淀粉样β蛋白25~35(0.01μmol/L、2μmol/L、20μmol/L)培养24h。
短句来源
     PC12 cell apoptosis was induced by β-amyloid 25-35 in 96-well culture plate and cells were harvested to assay their survival rate with MTT colorimetric assay.
     将收获好的96孔板以淀粉样β蛋白25~35诱导PC12细胞凋亡,采用MTT比色分析测定细胞存活率。
短句来源
     TEER increased significantly under co-culture condition from (66.1±13.3) Ωcm2 to (182.2±6.7) Ωcm2. Conclusion This micropore membrane culture plate insert can be easily made, on which BCEC culture can be successfully performed.
     共培养条件下TEER增幅明显 :由 (6 6 1± 1 3 3)Ωcm2 升至 (1 82 2± 6 7)Ωcm2 。
短句来源
     after 24 hours,each bone slicewas placed in a well of 24-well culture plate containingIL-2(50u/ml,100u/ml), TNF-α(10 ̄(-10) M,5× 10 ̄(-10)M,10(-9)M),PGE_2(100ng/ml ) in medium 199,orcontrol.
     TNF-α(10 ̄(-10)M,5×10 ̄(-10)M,10 ̄(-9)M); PGE_2(100ng/ml);
短句来源
     1. MC3T3 cells were seeded on cell culture plate(dish) with different surface modification(LD80-20, LD90-10,FN and PLL).
     (2).LD80-20和LD90-10对MC3T3细胞粘附性的影响
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  culture plate
The membrane, 2.45 cm in diameter, which is part of a commercially obtained presterilized culture insert, provides two chambers when placed in a regular six-well culture plate.
      
HL-60 cells transferred from serum-supplemented to serum-free culture medium initially bound to culture plate tightly and then released from the plate on increasing the culture time and resumed exponential growth after about 8 h lag.
      
The results suggested that the adhesion and proliferation of keratinocytes seeded on chitosan-gelatin membranes were same as on tissue culture plate, in which gelatin could modify the interaction between keratinocytes and chitosan membranes.
      
Objective: Our goal was to assess a 12-well oocyte collection and embryo culture plate for use in the IVF laboratory.
      
Twelve-well culture plate for the efficient collection and culture of human oocytes and embryos
      
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The effects of estrone and estriol on the proliferation of marrow granulocytecommitted progenitor cells of normal mice are discussed in the paper.Progenitor cells in mouse bone marrow were assayed through their progeny inplate culture for 7 days.Both estrone and estriol have shown inhibitory effects onCFU-C(Colony Forming Unit Culture).When estrone with concentrations rangingfrom 10~(-12)M,10~(-8)M to 10~(-4)M,and estriol with concentrations ranging from10~(-13)M,10~(-9)M to 10~(-5)M,were added to the plates,there...

The effects of estrone and estriol on the proliferation of marrow granulocytecommitted progenitor cells of normal mice are discussed in the paper.Progenitor cells in mouse bone marrow were assayed through their progeny inplate culture for 7 days.Both estrone and estriol have shown inhibitory effects onCFU-C(Colony Forming Unit Culture).When estrone with concentrations rangingfrom 10~(-12)M,10~(-8)M to 10~(-4)M,and estriol with concentrations ranging from10~(-13)M,10~(-9)M to 10~(-5)M,were added to the plates,there showed striking decreaseof CFU-C in the culture plate.The inhibitory rate of each concentration was54.4%,50.9%,61.6% and 33.1%,36.3%,43.5% respectively during 7 days ofculture.The inhibitory effects of estrone and estriol were similar.During 5 days ofculture by mean of agar diffusion chamber (ADC),CFU-C Showed a 26.2% increasein the castrated mice compared with the control.The present work demonstrated that estrone and estriol exert inhibitory effectson CFU-C in Vitro and it is possible that estrogens have inhibitory effect onCFU-C in Vivo ADC.The latter needs further studying.

本文介绍了用“体外琼脂培养技术”和“体内扩散盒琼脂培养技术(ADC 法)”观察雌激素对小鼠骨髓粒系定向祖细胞集落产率(VCFU-C)的影响。体外培养的结果表明,雌激素对小鼠股骨骨髓中 CFU-C 的增殖具有抑制效应。

A serum microneutralization test using a cell-adapted Infectious Bursal Disease virus strain is reported in this paper. China-made polystyrene micro-culture plates, chicken embryo fibroblast culture and Earle' s lactalbumin hydrolysate nutrient medium were used in the test. Details of the modified procedure are described. The test has been found to be highly sensitive and will be a powerful tool in epidemiological survey of the disease in chicken flocks.The results of assessing the neutralizing antibody...

A serum microneutralization test using a cell-adapted Infectious Bursal Disease virus strain is reported in this paper. China-made polystyrene micro-culture plates, chicken embryo fibroblast culture and Earle' s lactalbumin hydrolysate nutrient medium were used in the test. Details of the modified procedure are described. The test has been found to be highly sensitive and will be a powerful tool in epidemiological survey of the disease in chicken flocks.The results of assessing the neutralizing antibody levels in a batch of artificially infected chickens demonstrates that it is most important to prevent IBD infection in chickens under 24 days old.

本文报道利用鸡传染性囊病(IBD)国外引入的一株细胞适应病毒而进行的微量血清中和试验。采用了国产的聚苯乙烯微量培养板、鸡胚成纤维细胞和Earle氏水解乳蛋白营养液。对方法的摸索过程作了较详细的描述。本试验敏感性高,对调查传染性囊病在鸡群里的流行情况是一个有力的武器。利用这个方法检测一批人工感染鸡的血清中和抗体水平的结果表明,对于传染性囊病来说,预防24日龄以下的幼鸡发病是最主要的。

A cloned F9-1 EC cell line whichis sensitive to retinoic acid inductionwas isolated from F9 EC cell line inour laboratory.A hypoxanthine phos-phoribozyl transferase deficient EC cellline-F9-1 aza was then selected inRPMI-1640 medium containing 20μg/ml 8-azaguanine after exposure toMNNG.By using PEG (50%W/V,M.W.4000) mediated cell fusion,F9-1 aza cells were fused with thymuscells of Wistar rat and then grown inHAT medium.After 7—10 days,thepresumptive hybrid colonies appearedand assumed a quite different morpho-logy...

A cloned F9-1 EC cell line whichis sensitive to retinoic acid inductionwas isolated from F9 EC cell line inour laboratory.A hypoxanthine phos-phoribozyl transferase deficient EC cellline-F9-1 aza was then selected inRPMI-1640 medium containing 20μg/ml 8-azaguanine after exposure toMNNG.By using PEG (50%W/V,M.W.4000) mediated cell fusion,F9-1 aza cells were fused with thymuscells of Wistar rat and then grown inHAT medium.After 7—10 days,thepresumptive hybrid colonies appearedand assumed a quite different morpho-logy from that of the parent EC cells,as widely separated round cells,epithe-loid cells or fibroblast-like cells (PlateⅠ,Figs.1—5).Each colony frequentlycontained different types of cells includ-ing bipolar and multipolar cells andintermediate forms.When they werelater picked up from wells in plasticplates and transfered into flasks,cellsfrom some colonies failed to grow,whileothers could do so.Those hybrid cellswhich could establish in vitro as celllines exhibited two major classes:(1)polygonal epitheloid cells and(2) fibro-blast-like cells. Our analysis was concerntratedon cells obtained from one colonyFRT-1 hybrid cells.The modal chro-mosome number of these hybrid cellswas found to be 80 (60—88),beingthus mostly 1:1 fusion (Plate Ⅱ.Figs.6.8.).By using C-banding technique,the karyotype clearly showed the pre-sence of a metacentric marker chromo-some of F9 cell whose centromerefailed to be stained and a number ofsmall metacentric or acrocentric chromo-somes contributed by rat thymocytes(Plate Ⅱ.Figs.7.8.).The isozymepatterns of lactate dehydrogenase(LDH) also showed the occurrence"hybrid"complexes of rat LDH-Aand mouse LDH-A subunits (PlateⅡ.Fig.9.). Furthermore,the FRT-1 hybridcells failed to grow into solid tumorswhen inoculated subcutaneously intoX-ray irradiated 129/Sv-ter mice andalso failed to grow in soft-agar culture(Plate Ⅱ,Figs.10,11.) From the above results,it can be ??concluded that the malignant pheno-type of F9-1 aza EC cell is suppressed insomatic hybrids between F9-aza cellsand rat thymus cells.In the literature,many authors have reported their workson the somatic hybridization of "nulli-potent" and multipotent EG cells withmouse thymus cells (references 2,4,9,10,11,12 and 13 in tablel).Theirresulting hybrid cells all tended to showEC cell phenotype and grew into tumorsafter subcutaneous inoculation intosyngenic mice.Clearly,these resultsare different from our data presentedin this paper.During the preparationof our manuscript,another paper ofTakagi (reference 14) come to our notice,in which he reported that somatic cellhybrids between a multipotent PSA-1EC cells and rat lymphocytes assumedphenotypes of differentiated cells.Therefore,the suppression of malignantphenotype of nulli-and multi-potentteratocarcinoma cells by rat thymusand lymphocyte genome merits furtherinvestigation.

利用抗8氮鸟嘌呤的F9-1 aza突变株细胞与大鼠胸腺细胞融合,在HAT培养基中成功地筛选到FRT杂交细胞。该种细胞与亲本细胞F9-1 aza的细胞形态完全不同,呈成纤维样、上皮样、圆形或多突起样的分化细胞形态。在扩大繁殖后主要为成纤维样或上皮样细胞。对其中一个集落FRT-1核型检查表明杂种细胞染色体众数为80(范围62—88),C分带显示??包含F9和大鼠胸腺细胞二个亲本的染色体。FRT-1细胞的乳酸脱氢酶图谱中出现由大鼠LDH-A亚基与小鼠LDH-A亚基组成的杂种酶带。该细胞接种到经x光照射的129/Sv-ter小鼠皮下不能长瘤,亦不能在软琼脂上生长,表明F9-1 aza细胞的恶性表型已受到大鼠胸腺细胞基因组的抑制。

 
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