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     The proliferation capability K562eGFP-bcr cells was inhibited at 48 hrs after transfection and decreased significantly 72 hrs later.
     K562eGFP·bcr细胞在体外培养48小时后增殖开始下降,72小时后细胞增殖下降较明显。
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     Immature cells were observed in crypt at 3-5 hrs after irradiation, and mitotic figures 11 hrs, and then multinucleares, atypical cells and mitotic figures 19-24 hrs later.
     看到照后3—5小时腺窝中有幼稚细胞出现,9—11小时有分裂相,19—24小时后有多核细胞,畸形细胞及核分裂相。
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     Both groups of male Wistar rats fed with common and vitamin E deficientdiets were administrated by oral gavage with TNT(1000mg/kg) in peanutoil,respectively,and decapitated 8 or 16 hrs later. The indices including GSHand phosphorylase a in liver homogenates,protein-SH and ~(45)Ca uptake ofmitochondria and microsomes were immediately assayed.
     分别给摄食普通饲料和维生素E缺乏饲料40天的两组雄性Wistar大鼠用TNT(1000mg/kg)花生油溶液灌胃染毒,观察8小时和16小时后肝匀浆GSH、磷酸化酶a、线粒体和微粒体蛋白巯基及~(45)Ca摄取的变化。
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     The 26 Haerbin rabbits were divided into two equal groups and supe ro vulated with 50IU and 100IU PMSG individually. 50IU hCG was injected through ear viena 48hrs later and surgical ova-flashing was done 50hrs later.
     分别用50IU和100IU两种剂量的PMSG对两组各13只成年哈白母兔进行超排处理,48小时后配种,同时耳静脉一次性注射人hCG50IU,52小时后手术冲卵。
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     5 hrs later,it diffused into the growth medium.
     5小时后扩散到培养基中。
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     Group B1 and group B2 were injected 99Tcm-rh-Annexin Ⅴ(5.55MBq/0.5μg/0.1ml/per mouse) intravenously 20hrs later, at the same time group B3 and group B4 received the same doses of 99Tcm without being labelled with Annexin Ⅴ.
     20h后,B1、B2组同时由鼠尾静脉注入99Tcm-rh-Annexin Ⅴ(剂量为5.55MBq/0.5μg/0.1ml/只),B3、B4组只注入相同放射性活度的高锝酸盐及还原剂,而不标记Annex-in Ⅴ;
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     ResultsXDI in 1%-5% concentration could induce rMSCs differentia ted into neuron-like cells, the inducing rate reached 83.5±3.8% 6-12 hrs later, more than 90% cells, survival rate was over 36 hrs.
     结果 1%~ 5 %香丹注射液的诱导组 ,均可诱导rMSCs分化为神经元样细胞 ,诱导后 6~ 12h诱导率可达 (83 5± 3 8) % ,90 %以上的细胞存活时间 >36h。
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     Methods The logarithmically growing CEM cells cultured in vitro (cell density 2×105/mL) were exposed to 0.1, 0.5, 1, 5, and 10 μM Dex, then were collected 24, 48 and 72 hrs later. Untreated CEM cells were used as Controls.
     方法将在体外培养的对数生长期的CEM细胞浓度调至2×105个/mL,接种于24孔培养板中,用终浓度分别为0.1,0.5,1,5,10μM地塞米松处理,以不加任何药物的CEM细胞作为对照组,培养后24,48,72h取样。
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     Anti-LA18 supernate (SN) was' collected from LA18 cell line afterbeing cultured 72 hrs later.
     LA18细胞株培养72hr后收集合Anti-LA18的上清;
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     After PCIA and TPIA,such indexes as morphina volume,VAS score,sedative degree,MAP,HR,RR,SPO 2,satisfactory degree had been recorded at 4,8,12,24 hrs later.
     于用药后 4、 8、 12、 2 4h进行观察评估各时段的吗啡用量 ,视觉模拟评分 (VAS) ,镇静程度 ,MAP、HR、RR、SPO2 ,患者满意度及副作用。
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     Later ,M.
     随后,M.
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     5 hrs later,it diffused into the growth medium.
     5小时后扩散到培养基中。
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     2 hrs later,the NSP transported towards PT.
     2小时后,新合成的蛋白质向花粉管方向转移;
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     In the later paper of J.
     在后来的文章【6】中J.
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     24 hrs.
     5mM丁酸钠作用24小时后,细胞体积增大,形态改变呈现上皮样;
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Each subject received a single drug injection, and tests for ambulatory activity and motor coordination were performed 1, 24, 48 and 72 hrs later.
      
Cellular lethality was assessed by measurement of lactate dehydrogenase (LDH) 24 hrs later.
      
Sciatic nerves were crushed in anaesthesized rats and 2 hrs later segments of nerve, including the site of the crush, were removed and incubated in media containing [3H]Arg.
      
A second white light flare is suspected on a photograph taken 43/4 hrs later.
      
day all animals received an intraperitoneal injection of 300 μC14C-acetate and were sacrified 4 hrs later.
      
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Trans-stilbene, a chemical reagent, was found to be effective in protecting the liver from GGl_4 injury. In the first series of experiments, mice were given orally two doses of stilbene (100 mg/kg for each dose) in the first day and a dose of GCl_4 (0.01 ml/kg) the next day. The SGPT and SGOT levels as well as BSP retention were significantly lower in stilbene pre-treated mice than in the GCl_4 controls determined 16 hrs after injection of the liver poison. Histological examination revealed that lesions of the...

Trans-stilbene, a chemical reagent, was found to be effective in protecting the liver from GGl_4 injury. In the first series of experiments, mice were given orally two doses of stilbene (100 mg/kg for each dose) in the first day and a dose of GCl_4 (0.01 ml/kg) the next day. The SGPT and SGOT levels as well as BSP retention were significantly lower in stilbene pre-treated mice than in the GCl_4 controls determined 16 hrs after injection of the liver poison. Histological examination revealed that lesions of the liver of stilbene pre-treated mice were less severe than those of GCl_4 controls. In the second series of experiments, a dose of GCl_4 was given to the mice and 40 hrs later the mice were given orally stilbene at dosages of 50 or 100 mg/kg twice a day for two days. The repair of injured liver tissue seemed to be accelerated since the liver weights and SGPT levels were statistically lower in the stilbene treated mice. These findings are parallel to the pathological observations.Since this compound did not show any effect in lowering either the SGPT levels or the GPT levels of liver tissue in normal mice at the dosage as high as 1000 mg/kg, the possibility of inhibition of the enzymes may be excluded.In the third series of experiments, mice fasted for 24 hrs were used. An hour after a dose of stilbene (100 or 200 mg/kg), mice were injected with 10% glucose (20ml/kg) and sacrificed 2 hours thereafter for the determination of liver glycogen. The results showed that glycogenesis was stimulated by the oral administration of stilbene. In adrenalectomized mice this action was even more significant: the liver glycogen in the stilbene (200 mg/kg) pre-treated mice was found to be twice as high as controls—a potency approximating 100 mg/kg of cortisone.No significant effect was found either on the pentobarbital sleeping time in mice (stilbene 200 mg/kg, p o) or on cotton pellet granuloma response in rats (stilbene 100 mg/kg/day p o for 7 days). However, the compound exhibited a very weak estrogenic activity as shown in the immature mice uterus assay.The toxicity of stilbene is low. When suspended in starch, the ip LD_(50) was found to be 6.50±0.76 g/kg in mice. No death was recorded in 10 mice given an oral dose of 8.0 g/kg. When dissolved in oil the toxicity of stilbene increased sharply, the po LD_(50) of its peanut oil solution was found tO he 0.92±0.14 g/kg. Four dogs (two males and two females) were fed stilbene 10~50 mg/kg for 30 days. No change in behavior, liver and kidney functions and blood counts was found.

芪(二苯乙烯)通常用于化学工业,对其药理活性的研究很少。通过动物实验我们发现芪能明显保护CCl_4引起的小鼠肝损害,表现在使高的SGPT及SGOT降低、BSP潴留减少、肝组织病变减轻等。芪明显促进肝糖元生成,此作用强度与可的松相近,但芪没有可的松那样的抗炎作用。在去肾上腺小鼠,芪仍能明显促进肝糖元生成,故此作用似乎不通过体内的垂体-肾上腺系统。芪对小鼠戊巴比妥睡眠时间影响不明显。根据实验资料分析,芪对CCl_4肝损害的保护机制大概不是通过酶诱导,也不象某些抗氧化剂那样直接对抗CCl_4的作用,但有可能与其促进肝糖元生成有某种关系。 芪有微弱的雌激素作用(约为己烯雌酚的数万分之一)。 芪的毒性很低,小鼠一次腹腔注射LD_(50)为6.5 g/kg,灌胃LD_(50)大于8 g/kg,但油溶液毒性较大(灌胃LD_(50)为0.92g/kg)。长期大量给予芪,对雄小鼠的生长及睾丸发育有抑制作用,给狗服芪50 mg/kg/天连续一个月以上未见明显异常反应。

Nine healthy volunteers received a single dose of 7.5mg/kg of Amikacin intramuscularly, Amikacin was rapidly absorbed from injection site. A peak serum level after 1 hr averaged 23.59/μg/nl, and declined to 12.77μg/ml 3 hrs later. 12 hrs after the administration of Amikacin in serum was 0.78/μg/ml. Pharmacokinetical parameters of Amikacin were determined by a least-squares. Analysed parameters were based on a two-compartment, open-system kinetic model. The halflife time of Amikacin was 2.48 hrs....

Nine healthy volunteers received a single dose of 7.5mg/kg of Amikacin intramuscularly, Amikacin was rapidly absorbed from injection site. A peak serum level after 1 hr averaged 23.59/μg/nl, and declined to 12.77μg/ml 3 hrs later. 12 hrs after the administration of Amikacin in serum was 0.78/μg/ml. Pharmacokinetical parameters of Amikacin were determined by a least-squares. Analysed parameters were based on a two-compartment, open-system kinetic model. The halflife time of Amikacin was 2.48 hrs. Apparent volumes of distribution averaged 22.6% of body weight, and the mean rate of renal clearance was 72.56ml/min. Of the administered dose, 92.28% was recovered from the urine in 24 hours.

九名健康人单次肌注丁胺卡那霉素7.5mg/kg,1小时后的高峰血浓度为23.58μg/ml,3小时的血清浓度为高峰血浓度的1/2(12.77μg/ml),12小时浓度降至0.78/μg/ml。药物主要从肾脏排出,24小时内排出给药量的92.28%,100μg/ml以上的尿浓度可维持12小时,最高可达1451μg/ml。血清浓度动力学按两室开放式模型分析,表观分布容积Vd13.56升,肾清除率Q72.56ml/min。根据动力学参数计算出的不同时间的血浓度与实测值非常接近。五名病毒性肝炎患者肌注相同剂量的丁胺卡那霉素后,血浓度与正常人的水平相接近。1例老年慢性病毒性肝炎肝硬变病人,肌注丁胺卡那霉崇7.5mg/kg后,血浓度明显高于正常人,血清消除半衰期为6.3小时,提示该类病人体内动力学过程有所改变。

Both of Harringtonme (natura and partial synthetic) and Homoharringtonine-delayed the traverse of G, to S phase in P388 murine leukemic cells. It was shown that under the action of these compounds, G1% increased and S% and G2+ M% decreased n DNA distribution histogram. The effects appeared soon after treat-men and disappeared 43 hrs later. Results suggest that these drugs inhibit protein synthesis and cause a delay in transition of leukemic cells from G1 to S phase, the latter being the main cause of inhibition...

Both of Harringtonme (natura and partial synthetic) and Homoharringtonine-delayed the traverse of G, to S phase in P388 murine leukemic cells. It was shown that under the action of these compounds, G1% increased and S% and G2+ M% decreased n DNA distribution histogram. The effects appeared soon after treat-men and disappeared 43 hrs later. Results suggest that these drugs inhibit protein synthesis and cause a delay in transition of leukemic cells from G1 to S phase, the latter being the main cause of inhibition of leukemic growth.

1/3LD_(50)剂量的三尖杉酯碱,高三尖杉酯碱和半合成三尖杉酯碱给药后都使G_1 期的P_(353)白血病细胞向 S期移行受到延迟,而引起G_1%增高,同时引起S%和 G_2+M%减少。48小时后基本恢复至对照水平。G_1 期进入S期的延迟可能与蛋白质合成受抑制有关。

 
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