A mutant of bacteriophage lambda A,Nam7 can not grow in Escherichia coli strain A19 (met,thi,his-95,rna-19,rel-1) since an amber mutation is harboured in λNam7 besides temperature sensitive mutation cI857.λcI857 grows well on E.
λ Nam 7除其cI基因是cI 857温度敏感突变外,其N基因携有amber无义突变,故在Eseherichia coli A19(met,thi,his-95,rna-19,rel-1)上不能增殖。
positive clones have been obtained from a human tonsil cell lambda gt 11 cDNA library screened with a mixture of McAb 5C5 and 5C5-G1 . The cDNAs of the positive clones were inserted into plasmid pUC1 8. The cDNAs were sequenced with .
There were 31 cas- es of IgG_1—G_4(68.9%),8 IgA_1—A_2(17.8%),2 IgM(4.4%),1 IgD(2.2%)and 3 light chain disease cases. The IgE type was not found and Kappa or Lambda light chain was de- tected in these cases.