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amplified band
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  扩增条带
     The brightness of VCAM 1 mRNA amplified band decreased significantly in Adm1 50( 1× 10- 8) mol/L group whereas slight brightness and obscure amplified band was found in Adm1 50( 1× 10- 7) mol/L group.
     肾上腺髓质素1-50(1×10-8)mol/L组血管细胞黏附分子1mRNA扩增条带亮度明显减弱。 肾上腺髓质素1-50(1×10-7)mol/L组仅为亮度微弱且模糊的扩增条带
短句来源
     1. PCR results showed that positive amplified band of tobacco K326 was obtained after RD29a-GUS and bivalent gene RD29a-CBF_3 and RD29a-COR15a were introduced, which proved that exogenous genes were successfully transformed into tobacco.
     1.分别将RD29a-GUS基因和RD29a-CBF_3与RD29a-COR15a双价基因导入烟草材料K326中,PCR扩增检测结果表明有阳性扩增条带,初步证明外源基因已经整合到烟草基因组中。
短句来源
     By using simple sequence repeat (SSR), 25 apple cultivars were used for genomic polymorphic analysis. 97 alleles were amplified by using 10 pairs of primers selected from 20 pairs, of which average 9.7 alleles per pair of primer were amplified and polymorphic percentage of amplified band was 56.4%-100%.
     利用SSR方法对苹果25个品种进行了基因组多态性分析,从20对引物中筛选出10对多态性引物用于正式扩增,一共扩增出97个位点,平均每对引物扩增出9·7个位点,扩增条带的多态性百分率在56·4%~100%之间。
短句来源
     Results Amplified band of ACE2 was detected by RT-PCR in the total RNA extracted from rat PMVEC.
     结果RT-PCR法从细胞总RNA中检测出ACE2的扩增条带;
短句来源
     MAIN OUTCOME MEASURES:The expression of myocardial VCAM 1 mRNA in control group and groups of different concentrations of Adm1 50.RESULTS:After electrophoresis,each group was found to have an obvious amplified band at 194bp,which was GADPH mRNA amplified segment,and the expression of GADPH mRNA in each group was the same.
     主要观察指标:对照组及不同浓度肾上腺髓质素1-50组心肌组织VCAM-1mRNA的表达。 结果:电泳后,各组在194bp处均可见一明显的扩增条带,此为甘油醛-3-磷酸脱氢酶mRNA扩增片段,各组间甘油醛-3-磷酸脱氢酶mRNA的表达基本一致。
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  扩增带
     Results Excellent specificity of the amplified products could be found from both standard and wild strains of EVC, O139VC and V. parahaemolyticus. It also means that no amplified band was detected from total 35 strains of other bacilli, including salmonella, comma bacillus which do not belong to O1 and O139 serogroups.
     结果用该方法对EVC、O139VC、副溶血性弧菌的标准菌株和野生株,扩增片段出现极好的特异性,而35株非O1非O139群霍乱弧菌、沙门氏菌等未见扩增带
短句来源
     13 selected Sangon 10 bp primers generated 64 bands,in which amplified band number by different primers ranged from 1(S60) to 8(S80),average band number amplified by each primer was 4.85.Genomic DNAs from 12 sites amplified by 11 primers generated same DNA bands,but genomic DNAs of the sample 3 amplified by S66 deleted one DNA band at 850 bp and sample 9 by S80 deleted one DNA band at 250 bp.
     平均4.85条。 其中11条引物对12个地方解放钟枇杷RAPD扩增结果都相同,而引物S66、S80对12个不同地方的解放钟枇杷进行RAPD扩增,结果表明3号在850bp、9号在250 bp处缺失1条扩增带,其余62条带都相同。
短句来源
     Twenty-three SSR primers gave stable amplified band pattern detected 100 alleles among the lines tested.
     用23对扩增带型稳定的SSR引物,从供试材料中检测出100个等位基因变异,每对引物检测等位基因2~10个,平均4.35个。
短句来源
     It was proven by sequencing that the 130 bp was the sheep SRY gene and male cell line, positive control had the amplified band.
     通过序列测定和同源性分析,证明PCR产物为SRY基因片段,说明有130bp扩增带的细胞系为雄性;
短句来源
     The highly specific and sensitive,Neisserta gonorrhoeae DNA diagnosis by polymerasechain reaction(PCR)shows one specific amplified band,when the purified DNA is only about 3.8fg,while nonspecific bands are found in other related bacteria.
     淋球菌PCR基因诊断方法特异、敏感,与泌尿生殖道的其它污染菌不产生非特异扩增反应,在淋球菌3.8fg提取DNA可得到明显扩增带
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  “amplified band”译为未确定词的双语例句
     Compared with the energy of Tm~(3+) excited states in other glass system,~3F_4 energy ofTm~(3+) in these glasses is considerable higher and ~3H_4 energy is considerable lower,so it can bepredicted that emission band of ~3H_4→~3F_4 transition is similar to the amplified band of gain-shiftTm~(3+) doped fiber amplifier.
     与在其它玻璃基质中相比,Tm~(3+)的~3F_4能级对应能量偏高,~3H_4能级对应能量偏低,使得~3H_4→~3F_4跃迁波长较大,接近于增益迁移光纤放大器的放大波长。
     Results The amplified band patterns are obviously different in the products of 4 RAPD primers (p2 、 p3 、 p5 and p6) and 4 STR primers (2、 4、 10、11).
     结果在6条RAPD引物中,p2、p3、p5和p6这四条引物扩增的条带出现差异,表现为不同的RAPD图谱; 在20对STR引物中,引物2、4、10和11,这四对引物扩增的条带出现差异,表现为不同的STR图谱。
     Results The amplified band patterns were apparently different in the products of 5 primers(p1,p2,p3,p4 and p6).
     结果在6条随机引物中,p1、p2、p3、p4和p6这5条引物扩增的条带差异较为明显,表现为不同的RAPD图谱。
短句来源
     Mitochondrial genome of Pol,Ogu and News lines Xin1(induced by radiation) were amplified with primer which was designed according to gene orf224.The results show Xin1 CMS has a amplified band which Pol has,and lack a band which Pol has.
     对新不育系Xin1、Polima和Ogu的orf224基因的扩增结果表明Xin1有1条与Pol相同的谱带,缺失Pol的另一条谱带;
短句来源
     To use specific expression of foreign genes of promoters in transgenic rice research,the rice rbcS promoter was isolated from the rice of ZhongHua11 genomic DNA by PCR,and its sequencing indicated that the amplified band(2 746 bp) was 99.2% homologous to the reported ones at the correspondent sequence regions.
     为将高效特异的启动子用于转基因水稻研究,利用PCR技术从水稻‘中花11’基因组DNA中克隆了rbcS启动子,序列分析表明,扩增片段(2 746 bp)与已报道的该基因序列相应区域的同源性达99.2%。
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  amplified band
The amplified band from the susceptible lines could, however, be discerned from that of the resistant ones after cleavage with the restriction enzyme Hind III.
      
PCR amplification with the specific primers resulted in an amplified band (SCAR) in both susceptible and resistant tomato lines.
      
RT-PCR produced a single band and sequence analysis confirmed that the amplified band was Δ9 desaturase.
      
One plant was homomorphic for an amplified band on 3RL which was stable over two generations.
      
PCR amplification with the 20-mer primers produced a single amplified band in both susceptible and resistant tomato lines.
      
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A nested polymerase chain reaction (PCR) technique for amplification of the hepatitis B virus DNA was established. Two steps of the reaction were included: in the first step the sequences were amplified by the outer primers, and products of the first amplification were reamplified by the nested primers in the second steps. This technique was found to be highly sensitive and capable of detecting HBV DNA up to l0-6 Pg level. The specificity of the amplified bands was confirmed by Southern blot hybridization...

A nested polymerase chain reaction (PCR) technique for amplification of the hepatitis B virus DNA was established. Two steps of the reaction were included: in the first step the sequences were amplified by the outer primers, and products of the first amplification were reamplified by the nested primers in the second steps. This technique was found to be highly sensitive and capable of detecting HBV DNA up to l0-6 Pg level. The specificity of the amplified bands was confirmed by Southern blot hybridization analysis using a radiolabled oligonucleotide probe and enzyme cutting by Bgl Ⅱ . Anti-HBe positive, anti-HBc positive alone, and anti-HBs positive alone sera were tested by this technique for HBV DNA, the results showed that the extremely low levels of HBV DNA in these sera were detected but couldn't be found, positive by standard PCR.

本研究建立一种Nested PCR技术检测HBV DNA,即采用内外两对引物分别进行连续两次扩增,使检测极限由一次PCR的10~(-2)Pg提高到10~(-5)Pg;经~(32)P标记寡核苷酸探针作Southern转移杂交以及BgIⅡ作酶切分析证实两次扩增均为特异性扩增。通过抗-HBe阳性、单项抗一HBc阳性和单项抗-HBs阳性三种血清的HBV DNA的检测,表明该方法确能检出标准PCR所不能检出的极低水平的HBV感染,对提高乙型肝炎的诊断水平及更准确地评价药物疗效有重要意义。

We have screened out a specific Toxoplasma gondii DNA fragment from a genomic DNA library of T.gondii (ZS2 strain).The partial sequences of the cloned segment were analysed.A specific primer pair of the oligonucleotides for the T.gondii DNA sequences has been designed and synthesized in our laboratory.Polyme- rase chain reaction for in vitro enzymatic amplifying the specific DNA sequence for T.gondii was established.A specific amplified band was shown in the PCR products from DNAs from four different strains...

We have screened out a specific Toxoplasma gondii DNA fragment from a genomic DNA library of T.gondii (ZS2 strain).The partial sequences of the cloned segment were analysed.A specific primer pair of the oligonucleotides for the T.gondii DNA sequences has been designed and synthesized in our laboratory.Polyme- rase chain reaction for in vitro enzymatic amplifying the specific DNA sequence for T.gondii was established.A specific amplified band was shown in the PCR products from DNAs from four different strains of T.gondii and peripheral white blood cells and thymus of three baby pigs artificially infected with T.gondii, but was not shown in DNAs from controls, ie., normal human and baby pig peripheral white blood cells, spleen of normal mouse, plasmodium falciparum, Pneumocystis carinii, Entameba histolytica and human cytomegalovirus.The amplified products were further analysed by southern blot and restriction pattern and shown to be T.gondii origin.As little as one parasite of T.gondii or one pg of purified DNA from T.gondii can be detected by the PCR method.The analysed DNA sequences of the cloned fragment and the designed primers are different from all the known DNA sequence in the gene bank.This is a simple, sensitive and rapid method for detecting T.gondii.

从弓形虫(ZS_2株)基因组文库中筛选出了一个弓形虫特异DNA片段的克隆,对克隆的片段进行了部分顺序分析。根据所得DNA顺序,自行设计并合成特异的寡核苷酸引物对,建立了体外扩增弓形虫特异DNA顺序的聚合酶链反应(PCR)方法。4种不同来源的弓形虫株DNA、人工感染弓形虫的三头幼猪白细胞和胸腺DNA经过PCR扩增,均出现特异的扩增条带;而正常人、正常幼猪外围血白细胞、正常小鼠脾脏、恶性疟原虫、卡氏肺孢子虫、溶组织内阿米巴和人巨细胞病毒DNA均不出现特异的扩增条带。对扩增产物进行了Southern印迹和限制性内切酶图谱分析,证明该PCR产物是弓形虫DNA特异的顺序。该方法可测出少至1pg的弓形虫DNA或1个弓形虫体的裂解液。本文分析的DNA顺序和设计合成的引物顺序数据,经电脑DNA数据库检索,证明无相同的顺序。本方法并具有简便、快速等优点,便于推广应用。

Based on the partial sequences of the specific DNA cloned fragment from T.gondii (ZS2 strain) a specific primer of the oligonudeotide for the Toxoplasma gondii DNA sequence has been designed and synthesized in our laboratory.The method of the DNA diagnosis for to-xoplasmosis by polymerase chain reaction (PCR) has been established.A specific amplified band was shown in the PCR products from DNAs of T.gondii and seven manifold terata.The DNAs from the peripheral blood leukocytes of fifty normal individuals...

Based on the partial sequences of the specific DNA cloned fragment from T.gondii (ZS2 strain) a specific primer of the oligonudeotide for the Toxoplasma gondii DNA sequence has been designed and synthesized in our laboratory.The method of the DNA diagnosis for to-xoplasmosis by polymerase chain reaction (PCR) has been established.A specific amplified band was shown in the PCR products from DNAs of T.gondii and seven manifold terata.The DNAs from the peripheral blood leukocytes of fifty normal individuals and seventy-five patients as infants with hepatitis syndrome and pregnant women with previous abnormal birth histories were diagnosed by PCR.Among the seventy-five diagnosed cases,ten were positive.The normal individuals all were negative.Using 32P-cloned T.gondii specific DNA fragment as probe and Southern blot assay,the results showed that the probe only hybridized to the speciific amplified DNA bands,but did not hybridize to the amplified DNA products of negative cases.Our PCR method is a rapid,highly specific and sensitive one for delecting toxoplasmosis as compared with DNA probing,immunoassay and animal inoculation.

根据本实验室筛选出的弓形虫(ZS_2株)特异DNA克隆片段的部分顺序分析的数据,设计并合成特异的寡核苷酸引物对,建立多聚酶链反应诊断弓形虫病的方法。不同来源的弓形虫株和7例畸形胎儿DNA经体外基因扩增,扩增产物经电泳检测,均出现特异的扩增条带。对42例肝炎综合症的婴儿和33例不良生育史的孕妇的外周血白细胞DNA检测,分别为6例和4例阳性。50例正常人外周血白细胞DNA检测均为阴性。对扩增产物进行Southern印迹分析,以~(32)P标记克隆的弓形虫特异DNA片段为探针,能与阳性病例特异的扩增条带杂交,而不与阴性样品的扩增产物杂交。本法并与其它检测方法的结果相比较,具高度特异、敏感且快速的优点。

 
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