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A method described here is capable of direct visualization of protease zymogram on the disc electrophoresis gel. The method is more sensitive and convenient in comparison with the ordinary zymogram technique by which visualization is carried out on a substrate plate after contacting with the electrophoresis gel slab and enzymatic reaction. The basic principle and procedure are as follows: (1) envelope the polyacrylamide gel after electrophoresis with a casein layer as substrate; (2) the enzymatic... A method described here is capable of direct visualization of protease zymogram on the disc electrophoresis gel. The method is more sensitive and convenient in comparison with the ordinary zymogram technique by which visualization is carried out on a substrate plate after contacting with the electrophoresis gel slab and enzymatic reaction. The basic principle and procedure are as follows: (1) envelope the polyacrylamide gel after electrophoresis with a casein layer as substrate; (2) the enzymatic reaction proceed at 43℃ and saturated humidity for two hours; (3) stain the gel to visualize the remaining casein as a background of the transparent protease zone. 本文介绍了在盘电泳聚丙烯酰胺凝胶上直接显示蛋白酶酶谱的方法,比通常的凝胶板重合到底物平板进行酶反应、显示的方法灵敏、方便.基本原理与操作步骤是将电泳后的聚丙烯酰胺盘电泳凝胶包上一层酪蛋白为底物,然后在饱和湿度与43℃条件中进行酶反应2小时,再染色显示未水解的酪蛋白背景,从而将透明的酶带区域衬托出来. A method described here is capable of direct visualization of protease zymogram on the disc electrophoresis gel. The method is more sensitive and convenient in comparison with the ordinary zymogram technique by which visualization is carried out on a substrate plate after contacting with the electrophoresis gel slab and enzymatic reaction. The basic principle and procedure are as follows: (1) envelope the polyacrylamide gel after electrophoresis with a casein layer as substrate; (2) the enzymatic... A method described here is capable of direct visualization of protease zymogram on the disc electrophoresis gel. The method is more sensitive and convenient in comparison with the ordinary zymogram technique by which visualization is carried out on a substrate plate after contacting with the electrophoresis gel slab and enzymatic reaction. The basic principle and procedure are as follows: (1) envelope the polyacrylamide gel after electrophoresis with a casein layer as substrate; (2) the enzymatic reaction proceed at 43℃ and saturated humidity for two hours; (3) stain the gel to visualize the remaining casein as a background of the transparent protease zone. 本文介绍了在盘电泳聚丙烯酰胺凝胶上直接显示蛋白酶酶谱的方法,比通常的凝胶板重合到底物平板进行酶反应、显示的方法灵敏、方便.基本原理与操作步骤是将电泳后的聚丙烯酰胺盘电泳凝胶包上一层酪蛋白为底物,然后在饱和湿度与43℃条件中进行酶反应2小时,再染色显示未水解的酪蛋白背景,从而将透明的酶带区域衬托出来. A brief description is made to the basic principle and procedures to perform the program of "ABSORW module method. And a tentative approach is made to the significance, function of the three absorption coefficients section and the way how to interpret them each using the results from the parameter test and the data processed. Also, the first fruit with respect to the geological effect has been reached in realizing the attempt to predict the lithologic characters and hydro-carbon utilizing the absorption... A brief description is made to the basic principle and procedures to perform the program of "ABSORW module method. And a tentative approach is made to the significance, function of the three absorption coefficients section and the way how to interpret them each using the results from the parameter test and the data processed. Also, the first fruit with respect to the geological effect has been reached in realizing the attempt to predict the lithologic characters and hydro-carbon utilizing the absorption coefficients data. 本文对提取吸收系数的“ABSOR”模块方法原理及程序实现步骤,作了简要的概述;并通过本方法的参数试验及实际资料处理,对所得三种吸收系数剖面的意义、作用及解释方法,进行了初步探讨。同时在应用吸收系数资料预测地层岩性、油气的尝试中,初步得到较好的地质效果。 实践表明,吸收系数在能源勘探中是一个很值得重视的参数,应进一步加以研究开发应用。
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