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cow nose
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  牛鼻
     Thefresh noncornified layers of cow nose epi-dermis were immersed in 0.1 mol/L citricacid-sodium citrate buffer (pH 2.3, con-taining 0.5mmol/L PMSF),and then trea-ted with homogenization and discontinuoussucrose density gradient centrifugation. Desmosomes were located at the 50-56%sucrose interface.
     取新鲜牛鼻表皮棘层,以pH2.3含0.5mmol/L苯甲基磺酰氟、0.1mol/L枸椽酸-枸椽酸钠缓冲液浸泡,匀浆,经蔗糖密度梯度离心,得到纯桥粒。
短句来源
     Spleen cells of BALB/c mice immunized with desmoplakin 11 which was prepared fromfresh cow nose epidermis were fused with myeloma cell line Sp2/0.Antibodies in culture supernatantof the hybridoma were detected by indirect ELISA and immunohistochemical analysis and POsitivehybridomas were cloned using liciting dilutions.
     本文用改良构椽酸-枸椽酸钠法,从新鲜牛鼻表皮制备的DesmoPlakinⅡ(DPⅡ)免疫BALB/C,小鼠,取其脾淋巴细胞在PEG介导下与SP2/0细胞融合,经筛选、克隆化获得4株能稳定分泌DPⅡMCAb的杂交癌细胞系(分别命名为DPy—1,2,3,4)。
短句来源
  “cow nose”译为未确定词的双语例句
     Desmosomes were isolated from cow nose epidermis. Desmoplakin KDPDwas purified from desmosomes by preparative electrophoresis.
     作者从水牛鼻表皮中分离桥粒,提取桥粒中的Desmoplakin I(DPI)。
短句来源
  相似匹配句对
     BEAUTY NOSE
     俏鼻保养秋令时
短句来源
     Electronic Nose
     电子鼻
短句来源
     Desmosomes were isolated from cow nose epidermis. Desmoplakin KDPDwas purified from desmosomes by preparative electrophoresis.
     作者从水牛鼻表皮中分离桥粒,提取桥粒中的Desmoplakin I(DPI)。
短句来源
     Studies on Cow Mastitis
     奶牛乳腺炎的研究
短句来源
     The Automatic Reconfiguration of COW
     工作站机群系统自动重构机制
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A simple method is repor-ted for the isolation of desmosomes. Thefresh noncornified layers of cow nose epi-dermis were immersed in 0.1 mol/L citricacid-sodium citrate buffer (pH 2.3, con-taining 0.5mmol/L PMSF),and then trea-ted with homogenization and discontinuoussucrose density gradient centrifugation.Desmosomes were located at the 50-56%sucrose interface. Electron microscopy re-vealed that the characteristic desmosomestructure was well preserved.and that afew intermediate filament bundles attachedto...

A simple method is repor-ted for the isolation of desmosomes. Thefresh noncornified layers of cow nose epi-dermis were immersed in 0.1 mol/L citricacid-sodium citrate buffer (pH 2.3, con-taining 0.5mmol/L PMSF),and then trea-ted with homogenization and discontinuoussucrose density gradient centrifugation.Desmosomes were located at the 50-56%sucrose interface. Electron microscopy re-vealed that the characteristic desmosomestructure was well preserved.and that afew intermediate filament bundles attachedto desmosome plaque were removed bythe solubilizing action of the buffer. Ap-proximate 100 mg desmosomes(dry weight)were got from 20g wet noncornified layersof epidermic tissue.

本文报道一种简单易行的桥粒提取方法。取新鲜牛鼻表皮棘层,以pH2.3含0.5mmol/L苯甲基磺酰氟、0.1mol/L枸椽酸-枸椽酸钠缓冲液浸泡,匀浆,经蔗糖密度梯度离心,得到纯桥粒。电镜证实桥粒结构保存完好,附在桥粒斑上的中丝部分消失。209牛鼻表皮棘层可提取桥粒约100mg。

Desmosomes were isolated from cow nose epidermis. Desmoplakin KDPDwas purified from desmosomes by preparative electrophoresis. The spleen cells of BALB/C mice immunized with DP I were fused with myeloma cells line SP2/0. Screening for antibody activity in cultured supernatants was carried out by ELISA and immunohistochemical staining on frozen sections of human skin. After positive hybridomas were subcloned three time by limiting dilution, a mouse hybridoma, AD-1, producing monoclonal antibody (McAb) to...

Desmosomes were isolated from cow nose epidermis. Desmoplakin KDPDwas purified from desmosomes by preparative electrophoresis. The spleen cells of BALB/C mice immunized with DP I were fused with myeloma cells line SP2/0. Screening for antibody activity in cultured supernatants was carried out by ELISA and immunohistochemical staining on frozen sections of human skin. After positive hybridomas were subcloned three time by limiting dilution, a mouse hybridoma, AD-1, producing monoclonal antibody (McAb) to DP I was established. The tissue specificity of the McAb was tested on normal tissue frozen sections with immunohistochemical technique. All the epithelia tested were stained positively with AD-1 McAb, whereas nonepithelia tested showed negative staining. It is suggested that AD-1 McAb may be used as immunohistochemical probe for differentiation of epithelial tumors and nonepithelial tumors.

作者从水牛鼻表皮中分离桥粒,提取桥粒中的Desmoplakin I(DPI)。用DPI作免疫原,免疫BALB/C小鼠,建立了一株分泌抗DPI单抗的细胞AD-1。免疫组化染色证实,该单抗与上皮组织呈阳性反应,与非上皮性组织呈阴性反应。提示该单抗可作为区分上皮性肿瘤与非上皮性肿瘤的免疫组化探针。

Spleen cells of BALB/c mice immunized with desmoplakin 11 which was prepared fromfresh cow nose epidermis were fused with myeloma cell line Sp2/0.Antibodies in culture supernatantof the hybridoma were detected by indirect ELISA and immunohistochemical analysis and POsitivehybridomas were cloned using liciting dilutions.The linmunoglobulin subclasses of four DP 11McAbs (DPy-1,2,3,4,)were IgGI and the number of chromosomes of 4 hybridoma cell lines rangedfrom 93-105.Immunohistochemical analysis showed all...

Spleen cells of BALB/c mice immunized with desmoplakin 11 which was prepared fromfresh cow nose epidermis were fused with myeloma cell line Sp2/0.Antibodies in culture supernatantof the hybridoma were detected by indirect ELISA and immunohistochemical analysis and POsitivehybridomas were cloned using liciting dilutions.The linmunoglobulin subclasses of four DP 11McAbs (DPy-1,2,3,4,)were IgGI and the number of chromosomes of 4 hybridoma cell lines rangedfrom 93-105.Immunohistochemical analysis showed all DPy-1,2,3,4 had high speCificity and sensitivity to epithelia and DP Ⅱ may not exist in epithelial cells of thyroid follicles. The POsitive reaction incytoplasm of glandular epithelial cells stained by ABC homunohistocllemical method with DP 11 McAbmay suggest that DP Ⅱ associated antigen existed in cytoplasm of glandular epithelial cells. The resultSalso showed that DP Ⅱ McAb be used not only in frozen section but also in routinly processed paraffinsection.It is believed that DP Ⅱ McAb is a very useful probe for pathological diagnosis and research onmechanism of tumors derived from epithelial tissues.

本文用改良构椽酸-枸椽酸钠法,从新鲜牛鼻表皮制备的DesmoPlakinⅡ(DPⅡ)免疫BALB/C,小鼠,取其脾淋巴细胞在PEG介导下与SP2/0细胞融合,经筛选、克隆化获得4株能稳定分泌DPⅡMCAb的杂交癌细胞系(分别命名为DPy—1,2,3,4)。4株McAb均为ⅠgGI,染色体数目为93~105.免疫组化分析表明,4株DPⅡMcAb具有很高的上皮组织特异性和敏感性,并提示DPⅡ可能不存在于甲状腺滤泡上皮组织中。腺上皮细胞胞浆内DPⅡMcAb免疫组化染色阳性,可能是腺上皮细胞胞浆内存在DPⅡ相关抗原的结果。实验表明DPⅡMcAb既可用于冰冻切片,又可用于石蜡切片,为上皮源性肿瘤的病理诊断和发生机理研究提供了一种有用的探针.

 
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