助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   restriction analysis results 的翻译结果: 查询用时:0.194秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

restriction analysis results
相关语句
  酶切结果
     Results Sequencing and restriction analysis results indicated ALT2 gene was cloned in frame into pET-28a.
     结果 重组质粒pET2 8 ALT2测序和酶切结果与预期完全符合。
短句来源
  “restriction analysis results”译为未确定词的双语例句
     The restriction analysis results showed that the fusion gene cecropin B - human lysozyme was ligated correctly into pET32a.
     经测序及酶切鉴定融合基因Cecropin B-人溶菌酶已经正确的连接到pET32a上。
短句来源
     The restriction analysis results showed that the RScDNA gene was ligated correctly into pPIC3.5K. With the electricity dash the recombinant plasmid was switch to the Pichia. pasteoris strain GS115. The strain with recombinant plasmid was chosen by PCR.
     用电激法将重组以后的酵母表达载体pPIC3.5KL导入甲醇型酵母(Pichia. pasteoris)菌株GS115中,获得转化子,对转化子进行组氨酸缺陷筛选和PCR筛选,证明目的基因RScDNA已经成功得整合到了酵母的染色体上。
短句来源
  相似匹配句对
     (N, W) Analysis
     关于点系结构的分析
短句来源
     C -- M. S analysis.
     —M. S.
短句来源
     Analysis of Language Form of Concept Restriction
     概念限制的语言形式分析
短句来源
     Simultaneity, restriction sites analysis was done.
     同时还对该基因的酶切位点进行了分析。
短句来源
     The results of restriction endonuclease analysis were in accordance with the expected.
     26株SS2的扩增片段经HincⅡ酶切鉴定,与预期结果一致。
短句来源
查询“restriction analysis results”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  restriction analysis results
Automated sequencing without a visual inspection often failed to detect existing heterogeneity within sequences, resulting in a discrepancy between the sequencing and restriction analysis results.
      
Restriction analysis results showed that 8/26 adenovirus strains contained two different FAdV types.
      
Restriction analysis results showed that 26 adenovirus strains contained two different FAdV types.
      


Objective (1)To determine the prevalence of Helicobacter pylori strains resistant to clarithromycin in Peking (2)To determine the genetic mutation in relation to clarithromycin resistance. Methods Helicobacter pylori were cultured from gastric biopsies obtained from 89 patients during upper endoscopy Minimal inhibitory concentrations of clarithromycin were determined by Etest method Genomic DNA was prepared by the hexadecyltrimethylammonium bromide (CTAB) phenol extraction method Mutations...

Objective (1)To determine the prevalence of Helicobacter pylori strains resistant to clarithromycin in Peking (2)To determine the genetic mutation in relation to clarithromycin resistance. Methods Helicobacter pylori were cultured from gastric biopsies obtained from 89 patients during upper endoscopy Minimal inhibitory concentrations of clarithromycin were determined by Etest method Genomic DNA was prepared by the hexadecyltrimethylammonium bromide (CTAB) phenol extraction method Mutations in clarithromycin resistant strains were identified by polymerase chain reaction and restrictions analysis Results (1)The prevalence of Helicobacter pylori strains resistant to clarithromycin was 13 5% in Beijing (2)All twelve clarithromycin resistant strains had the new BsaI site which is characteristic of the A2143G point mutation in 23S rRNA gene, none of the tested twenty four susceptible strains had the A2142G or A2143G mutation Conclusion The prevalence of clarithromycin resistant strains was not rare in Beijing Mutations in 23S rRNA are associated with clarithromycin resistance in Helicobacter pylori

目的  (1)确定北京地区幽门螺杆菌 (Helicobacterpylori,Hp)菌株对克拉霉素的耐药情况。 (2 )研究幽门螺杆菌对克拉霉素耐药与 2 3SrRNA基因点突变的关系。方法 从北京地区 89例有上胃肠症状的患者取得胃活检组织 ,微需氧培养得到Hp ,E 检验方法测定克拉霉素的最低抑菌浓度 (MIC) ,CTAB/NaCl方法提取敏感菌和耐药菌的DNA ,用限制性片段长度多态性 (PCR RFLP)检测克拉霉素耐药菌株的点突变。结果  (1)北京地区Hp菌株对克拉霉素的耐药率是 13 5 %。 (2 ) 12个克拉霉素耐药的Hp菌株均存在 2 3SrRNA基因的A2 143G点突变 ,进行PCR RFLP的 2 4个敏感菌株均无2 3SrRNA的点突变。结论 北京地区克拉霉素耐药的Hp菌株较为常见 ,Hp对克拉霉素的耐药与2 3SrRNA基因的点突变有关。

Objective To construct a recombinant plasmid for expression of human alanine aminotransferase Ⅱ (ALT2).Methods ALT2 cDNA was amplified by RT-PCR from total RNA of human liver,and cloned into pET-28a by restriction and subsequent ligation.Expression of ALT2 was induced by IPTG and demonstrated by SDS-PAGE,enzyme reaction and western blot.Results Sequencing and restriction analysis results indicated ALT2 gene was cloned in frame into pET-28a.Western blot showed a band with molecular weight of 58 kD reacted...

Objective To construct a recombinant plasmid for expression of human alanine aminotransferase Ⅱ (ALT2).Methods ALT2 cDNA was amplified by RT-PCR from total RNA of human liver,and cloned into pET-28a by restriction and subsequent ligation.Expression of ALT2 was induced by IPTG and demonstrated by SDS-PAGE,enzyme reaction and western blot.Results Sequencing and restriction analysis results indicated ALT2 gene was cloned in frame into pET-28a.Western blot showed a band with molecular weight of 58 kD reacted with anti-ALT1 serum.Enzyme dete rmination and activity staining in native PAGE indicated a high,soluble expression of ALT activity in the recombinant plasmid.Conclusion Human ALT2 gene was cloned and ALT2 protein with native activity expressed in satisfactory level.ALT2 shares some homology with ALT1.

目的 构建基因工程菌株 ,以获得重组人II型丙氨酸氨基转移酶 (ALT2 )蛋白。方法 逆转录PCR法从人肌肉组织总RNA中扩增ALT2基因片段 ,插入原核表达载体pET - 2 8a ,构建成融合表达质粒pET2 8 ALT2 ,将其转化大肠杆菌BL2 1DE3,IPTG诱导表达。SDS PAGE、活力测定、活性染色及westernblot鉴定表达产物。结果 重组质粒pET2 8 ALT2测序和酶切结果与预期完全符合。IPTG诱导后 ,阳性菌体裂解物上清ALT2活性很高 ,PAGE出现一分子量 5 80 0 0蛋白条带 ,可被抗ALT1抗血清识别 ,酶活性染色显示有ALT活性。结论 已成功将ALT2基因克隆到pET 2 8a载体 ,ALT2蛋白得到可溶性高效表达。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关restriction analysis results的内容
在知识搜索中查有关restriction analysis results的内容
在数字搜索中查有关restriction analysis results的内容
在概念知识元中查有关restriction analysis results的内容
在学术趋势中查有关restriction analysis results的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社