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antisense group
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  反义组
     RESULTS:The EAAC1 expression in the ischemic region of the model group(0.61±0.03) was significantly higher than that of the sham operated group(0.20±0.01)(F=49.49,P< 0.01),however, it was significantly lower in the antisense group(0.31±0.01) than the model group(F=49.33,P< 0.05).
     结果:模型组缺血区EAAC1表达(0.61±0.03)明显高于假手术组(0.20±0.01),差异有显著性意义(F=49.49,P<0.01),而反义组表达(0.31±0.01)低于模型组,差异有显著性意义(F=49.33,P<0.05)。
短句来源
     FN concentrations in the culture medium of high glucose group, antisense group, sense group and random group were (84.19±6.81) ng/ml, (57.65±5.05) ng/ml, (78.72±9.13) ng/ml and (77.13±7.36) ng/ml respectively.
     高糖组、反义组、正义组和随机组培养上清液中FN含量(ng/ml)分别为84.19±6.81、57.65±5.05、78.72±9.13、77.13±7.36;
短句来源
     Compared with the sense group,the mRNA expression and the protein expression of HIF-1α and TGF-3β decreased in antisense group.
     与HIF 1α正 义组比较,HIF 1α反义组HIF 1αmRNA和蛋白表达降低, TGF 3βmRNA和蛋白表达也下降.
短句来源
     ②The numbers of c-Fos protein positive cells in t he CA1,CA3 and dentate gyrus of hippocampus were 16.33±6.18,27.00±9.72 and 109 .67±23.44 in the antisense group,significantly lower than those in the missense and saline groups, but there was no difference between the missense and saline groups.
     ②反义组大鼠海马c-Fos蛋白阳性细胞数在海马CA1,CA3和齿状回分别为16.33±6.18,27.00±9.72和109.67±23.44,均低于生理盐水组和错义组,而错义组与生理盐水组之间c-Fos蛋白表达水平差异无显著性意义。
短句来源
     Compared with the sense group, the mRNA expression of HIF-1α and TGF-3β decreased in antisense group, as well as the protein expression of HIF-1α and TGF-3β.
     与HIF- 1α正义组比较,HIF- 1α反义组HIF -1αmRNA和蛋白表达降低,TGF- 3βmRNA和蛋白表达也下降。
短句来源
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  “antisense group”译为未确定词的双语例句
     Results(1)The apoptosis rates of the non-transfected group,transfect pcDNA3 group,sense group and antisense group in the culture medium of high glucose withact PD98059,were(12.10±2.21)%,(11.90±3.34)%,(5.50±0.50)%and(20.70±3.41)%,respectively.
     结果(1)未加PD98059的未转染组、空载体组、正义转染组及反义转染组各组细胞的凋亡率分别为(12.10±2.21)%、(11.90±3.34)%、(5.50±0.50)%和(20.70±3.41)%;
短句来源
     It was lower in the antisense group than that in the sense group, and the differences were obvious (which were respectively 348.55±39.63,752.11±76.43,P < 0.05 ).
     反义寡核苷酸组低于正义寡核苷酸组,差异有显著性意义(分别为348.55±39.63,752.11±76.43,P<0.05);
短句来源
     ① In antisense group, decoy group and decoy plus antisense group, the expression of proliferating cell nuclear antigen (PCNA) were lower than that in the positive control group, and the differences were significant (respectively were 348.55±39.63,369.05±71.56,309.33±12.86,635.16±89.95,P < 0.05).
     ①核因子κB的反义寡核苷酸组、诱骗性寡核苷酸组、反义寡核苷酸加诱骗性寡核苷酸组细胞核蛋白增殖细胞核抗原的表达低于阳性对照组,差异均有显著性意义(分别为348.55±39.63,369.05±71.56,309.33±12.86,635.16±89.95,P<0.05);
短句来源
     RESULTS: The Ox-LDL+antisense group cells contained significantly lower cholesteryl ester (19.9±1.9) mg/g (protein) than that of cells in Ox-LDL group (46.6±3.4) mg/g (protein) at 4 days.
     结果:72h后,Ox-LDL+antisense组细胞内胆固醇酯(19.9±1.9)mg/gprotein显著低于Ox-LDL组(46.6±3.4)mg/gprotein。
短句来源
     At day 2, the relative ACAT activity in Ox-LDL+antisense group was significantly lower than that in Ox-LDL group. Correlative analysis between activity of ACAT and adipophilin expression showed than R2 were 0.6176 and 0.8212 (P<0.05) in Ox-LDL group and Ox-LDL+antisense group, respectively.
     ACAT的活性与脂肪分化相关蛋白蛋白的表达量相关分析表明,Ox-LDL组R2=0.6176(P<0.05),Ox-LDL+antisense组R2=0.8212(P<0.05)。
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  相似匹配句对
     In group K.
     K.
短句来源
     Group C.
     实验动物随机分为3组,每组9只。
短句来源
     group 3: soluble stent with antisense-ODN;
     组3:植入反义c-m yc寡核苷酸可溶性支架;
短句来源
     There were no obvious changes in non-antisense oligomers treated group.
     错义序列对照组则无明显变化。
短句来源
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  antisense group
Treated arteries in the antisense group presented significantly lower EMI (p >amp;lt; 0.02), SMI (p >amp;lt; 0.02), and R (p >amp;lt; 0.01) than treated arteries in the other groups whereas the thickness and mean surface of the media were comparable.
      
In the antisense group mean all three values were significantly lower on the treated side than the control side (EMI: p >amp;lt; 0.02, SMI: p >amp;lt; 0.02, and R: p >amp;lt; 0.01).
      
Thirty nude mice were randomly divided into 3 groups: PBS control group (group A), scramble-sense control group (group B) and antisense group (group C).
      
The least inhibition in the antisense group was observed in donors 3 and 10.
      
Similarly, ovarian replacement by tumor cells was much less marked in the antisense group.
      
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Objective: To investigate the effect of BHRF1 antisense oligonucleotide on apoptotic resistance of the BHRF1 expressing cell line of nasopharyngeal carcimona, CNE2. Methods: After incubated with BHRF1 antisense oligonucleotide, BHRF1 CNE2 cells were treated with camptothecin, and tested for the alterations of proliferative ability and apoptotic index. Results: After the treatment of camptothecin, in antisense group, proliferative rates of CNE2 dropped and apoptotic rates increased. Conclusion: Antisense...

Objective: To investigate the effect of BHRF1 antisense oligonucleotide on apoptotic resistance of the BHRF1 expressing cell line of nasopharyngeal carcimona, CNE2. Methods: After incubated with BHRF1 antisense oligonucleotide, BHRF1 CNE2 cells were treated with camptothecin, and tested for the alterations of proliferative ability and apoptotic index. Results: After the treatment of camptothecin, in antisense group, proliferative rates of CNE2 dropped and apoptotic rates increased. Conclusion: Antisense inhibition of BHRF1 expression can reduce apoptotic resistance of CNE 2 cells, which may be valuable for the treatment of nasopharyngeal carcinoma.

目的:探讨BHRF1 反义寡核苷酸对表达BHRF1 的鼻咽癌细胞株CNE2 生存能力的影响。方法:检测反义阻断BHRF1 表达后BHRF1-CNE2细胞在喜树碱作用下增殖能力和凋亡指数的变化。结果:在喜树碱作用下,应用BHRF1 反义寡核苷酸组的癌细胞较对照组的增殖率下降,凋亡率增高。结论:反义阻断BHRF1的表达可降低CNE2细胞的生存能力,这在鼻咽癌的治疗中可能具有一定的价值

Objective: To evaluate the inhibitory effect of antisense phosphorothioate c myc oligodeoxynucleotide on intimal hyperplasia in autogenous vein graft.Methods:Thirty male New Zealand rabbits with external jugular veins interposed between ipsilateral common carotid arteries were randomly and equally divided into 5 groups.The antisense,sense and mismatch c myc oligodeoxynucleotide of 330 μg were dissolved in Fibrin glue and applied to the adventitia of vein graft. Twenty eight days after intervention,the animals...

Objective: To evaluate the inhibitory effect of antisense phosphorothioate c myc oligodeoxynucleotide on intimal hyperplasia in autogenous vein graft.Methods:Thirty male New Zealand rabbits with external jugular veins interposed between ipsilateral common carotid arteries were randomly and equally divided into 5 groups.The antisense,sense and mismatch c myc oligodeoxynucleotide of 330 μg were dissolved in Fibrin glue and applied to the adventitia of vein graft. Twenty eight days after intervention,the animals were sacrificed and defined segments of vein grafts were sectioned and observed under microscope,the image was digitalized by computer video analysis system, the intimal thickness and area,medial thickness and area and their ratios were calculated and analyzed statistically among groups.Results:The intimal thickness and area in antisense group decreased to 63.23% and 51.78% of controls,respestively, while the intimal thickness and intimal area had no significant deviation among control,Fibrin glue,sense and mismatch groups.The medial thickness and area among each group were compared without significant deviation.Conclusion:Antisense c myc oligodeoxynucleotide can significantly suppress the intimal hyperplasia in vein graft by locally delivered with Fibrin glue.The investigation provides the basis for the early clinical trials assessing the therapeutic potential of antisense c myc approach for the prevention of restenosis after vein grafting.

目的:研究局部应用反义寡核苷酸c-m yc对自体静脉移植物内膜增生的防治作用,为反义技术防治血管内膜增生的早期临床应用提供实验依据。方法:30 只新西兰兔颈外静脉间置于同侧颈总动脉后随机分成对照组、蛋白胶组、正义组、反义组及错配组,以医用生物蛋白胶分别溶解反义、正义及错配的c-m yc基因片段(330 μg)后直接涂于静脉移植物外膜。术后28d 取材制片,显微镜进行形态观察,并用计算机图像分析系统测量和计算内膜厚度、中膜厚度及二者之比值,内膜面积、中膜面积及二者之比值,以q 检验行统计学分析。结果:反义组增生内膜厚度较对照组降低36.77% ,内膜面积降低48.22% ,对照组、蛋白胶组、正义组及错配组间内膜厚度及内膜面积均无明显差异(P> 0.05);各组间中膜厚度及中膜面积均无明显差异(P> 0.05)。结论:医用生物蛋白胶携载反义寡核苷酸c-m yc局部用于静脉移植物外膜能显著防治其内膜增生,有效防止管腔狭窄。

Objective To quantitatively analyse the inhibitory effect of antisense phosphorthioate oligodeoxynucleotides on rat carotid artery after nitrogen drying injury by compute image processing.Method The rats were classified into six groups (antisense group,sense group,mismatch group,DOTAP group. control group and false group). The morphology of intimal hyperplasis in rat carotid artery after nitrogen drying injury were measured. Results The intimal hyperplasia in antisense...

Objective To quantitatively analyse the inhibitory effect of antisense phosphorthioate oligodeoxynucleotides on rat carotid artery after nitrogen drying injury by compute image processing.Method The rats were classified into six groups (antisense group,sense group,mismatch group,DOTAP group. control group and false group). The morphology of intimal hyperplasis in rat carotid artery after nitrogen drying injury were measured. Results The intimal hyperplasia in antisense ATR ODNs treated rats were inhibited significantly compared with the sense,mismatch and DOTAP group,whereas sense.mismatch and DOTAP group failed to show any inhibitory effect.Conclusion The ATR plays an important role in the mechanism of intimal hyperplasia,and it shows inhibitony effect of antisense phosphorthioate oligodeoxynucleotides on in rat carotid artery after nitrogen drying injury.

 目的 利用计算机图像处理分析系统对局部应用反义寡脱氧核苷酸( ODNs) 对大鼠颈动脉损伤后内膜增生抑制作用进行定量分析。方法 将大鼠随机分成6 组( 即反义组A、正义组B、错配组C、对照组D、脂质体组E、假手术组F) ,进行大鼠颈动脉损伤后内膜增生的形态参数测定。结果 A 组管腔面积大于B、C、D 及E 组( P< 0 .05) 且小于F 组( P< 0 .05) ;A 组的内膜面积、内膜/ 中膜面积比值、最大内膜厚度、内膜/ 中膜厚度比值、内膜覆盖率均明显小于B、C、D 及E组( P< 0 .01) ,而B、C、D 及E 组组间两两比较无显著性差异( P> 0 .05) 。结论 利用计算机图像处理从量化角度分析内膜增生的各种情况,进一步证实了血管紧张素Ⅱ1 型受体( AT1R) 的反义( ODNs) 对氮气干燥后大鼠颈动脉内膜增殖有抑制作用,且具有序列依赖性

 
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