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inter-simple
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     Randomamplified polymorphic DNA(RAPD)and inter-simple sequence repeat(ISSR)methods were applied to detectgenetic variation and relationship of3mangrove species of Bruguiera(Bruguiera gymnorrhiza,B.sexangul,B.sexangula var.rhynchopetala).
     用随机扩增多态性DNA(RAPD)和inter-简单重复序列(ISSR)分子标记技术对木榄属(Bruguiera)3种红树木榄(Bruguieragymnorrhiza)、海莲(B.sexangula)、尖瓣海莲(B.sexangulavar.rhynchopetala)进行遗传亲缘关系研究。
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  “inter-simple”译为未确定词的双语例句
     Objective:In order to compare the result extracting genomic DNA from Cynoglossus semilaevis Gtinther by using CTAB and STE,and then apply a new kind of molecular marker,which is Inter-simple sequence repeat(ISSR),to Cynoglossus semilaevis Gtinther genetic analysis.
     目的:比较CTAB法和STE法提取半滑舌鳎基因组DNA的效果,通过优化半滑舌鳎ISSR-PCR反应体系,将新型分子标记简单重复间序列(Inter-simple sequence repeat,ISSR),引用到半滑舌鳎遗传多样性研究中。
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     The effects of the concentrations of PCR reaction components on inter-simple sequence repeat (ISSR) amplification results of apricot cv. Qingmisha were studied with the primer UBC825〔primer sequence: (AC)8T〕.
     以杏品种清密沙为试材,用引物UBC825〔序列为(AC)8T〕研究了PCR反应体系的主要成分及退火温度对杏ISSR扩增结果的影响。 结果表明:Primer、Mg2+、Taq酶浓度对扩增效果有明显影响,而模板DNA和dNTPs含量对扩增结果影响不大。
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     Establishment of the Inter-simple Sequence Repeats Reaction System in Plum Germplasm
     李种质资源ISSR反应体系的建立
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     Inter-simple sequence repeats (ISSR) marker was used to analyze the genetic diversity and differentiation of 137 individuals within and among 7 populations of Orchidantha chinensis, an endangered species, in Guangdong Province.
     采用简单重复序列区间(ISSR,Inter-Simple Sequence Repeat)分子标记技术,对采自广东省的濒危植物兰花蕉(Orchidantha chinensisT.L.Wu)的7个居群137个个体进行遗传变异分析。
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     Inter-simple sequence repeat (ISSR) is a microsatellite-based technique, with the superiorities of simple, quick, reliable, and generating higher levels of DNA polymorphism, and being used as a new molecular marker for genetic study.
     其中,简单序列重复区间标记(inter-simple sequence repeat,ISSR)是一种基于微卫星序列发展起来的新型分子标记,具有简便、快捷、结果稳定和DNA多态性高等优点.
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     The impact is inter-related.
     多元化的这两个方面对公司价值有交互影响。
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     Inter-subjectivity and Education
     论主体间性及其教育
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     The status of optical inter(?)
     本文评述了光互连技术在圆片规模集成电路中的应用现状.
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     Successful Ist Inter TEXTILE
     首届中国国际纺织面料及辅料博览会圆满成功
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     (c) it is simple.
     (c)简单 ;
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  inter-simple
Genetic diversity of wild Cymbidium goeringii (Orchidaceae) populations from Hubei based on Inter-simple sequence repeats analys
      
In the present study, we investigated the distribution of genetic variation within and between eleven populations of Cymbidium goeringii in central China by using Inter-simple sequence repeats (ISSR) markers.
      
We investigated the genetic variation within 32 accessions distributed to 14 species and one variety by using ISSR (inter-simple sequence repeat) markers.
      
The procedure employs three methods of DNA analysis based on polymerase chain reaction (PCR): PCR with arbitrary primers (AP PCR), simple sequence repeat polymorphism (SSRP) analysis, and inter-simple sequence repeat (ISSR) analysis.
      
Inter-simple sequence repeat (ISSR) analysis was for the first time used to study the genetic diversity and phylogenetic relationships in 54 wild accessions and cultivars of the genus Lycopersicon.
      
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Random amplified polymorphic DNA (RAPD) and inter_simple sequence repeat (ISSR) methods were applied to detect genetic variation of 20 bulking samples and two individually sampled populations of Oryza granulata (Nees et Arn. ex Watt.) from China (M5 from Hainan Province and M27 from Yunnan Province). For the bulking sampled populations, 20 RAPD and 12 ISSR primers generated 209 and 122 bands, of which 134 (64.11%) and 89 (72.95%) were polymorphic respectively. For population M5, 146 and 95 bands were generated...

Random amplified polymorphic DNA (RAPD) and inter_simple sequence repeat (ISSR) methods were applied to detect genetic variation of 20 bulking samples and two individually sampled populations of Oryza granulata (Nees et Arn. ex Watt.) from China (M5 from Hainan Province and M27 from Yunnan Province). For the bulking sampled populations, 20 RAPD and 12 ISSR primers generated 209 and 122 bands, of which 134 (64.11%) and 89 (72.95%) were polymorphic respectively. For population M5, 146 and 95 bands were generated with 21.48% and 34.78% being polymorphic (PPB). For population M27, 151 (PPB = 17.22%) and 94 (PPB = 19.15%) bands of RAPD and ISSR were obtained. The results indicated that the level of genetic variation of O. granulata was lower than other species detected in the genus Oryza. UPGMA cluster based on genetic similarity and principle component analyses (PCA) based on band patterns divided the populations into two groups corresponding to their sources, which revealed that the majority of genetic variation of O. granulata occurred between Hainan and Yunnan. Consequently, more populations of the species should be considered for a reasonable conservation management. Comparison between the two marker systems showed that ISSR was better than RAPD in terms of reproducibility and ability of detecting genetic polymorphism. Mantel test revealed that the goodness of fit between them was significant (r=0.917, t= 12.718 ) when detecting genetic diversity at species level, but was poor at population level (r<0.200). The discrepancy was considered as the facts that different fragments were targeted by RAPD and ISSR, and that evolutionary process of O. granulata varied at different hierarchical levels.

用随机扩增多态DNA (RAPD)和inter_简单重复序列 (ISSR)标记对分别来自中国海南和云南 2 0个居群的疣粒野生稻 (Oryzagranulata (NeesetArn .exWatt.) )混合样品 ,以及海南 (M5 )和云南 (M2 7) 2个居群各 2 0个植株的遗传多样性进行了检测。在混合取样的居群中 ,2 0个RAPD引物和 12个ISSR引物分别扩增出 2 0 9和 12 2条带 ,多态条带比率 (PPB)分别为 6 4.11%和 72 .95 %。对于分单株取样的居群 ,在M5中 ,两种标记分别扩增出 146和 95条带 ,PPB值分别为 2 1.48%和 34 .78%。在居群M2 7中 ,则分别扩增出 15 1(PPB =17.2 2 % )和 94(PPB =19.15 % )条带。结果表明 ,中国的疣粒野生稻在物种水平的遗传多样性较低 ,其遗传变异主要存在于海南和云南两地之间 ,而在居群内的遗传多样性水平很低。基于Jaccard遗传相似性系数的UPGMA聚类和基于多态条带表型的主成分分析 (PCA)都将中国的疣粒野生稻分为来自海南和云南两类 ,两地间该物种的平均遗传相似性...

用随机扩增多态DNA (RAPD)和inter_简单重复序列 (ISSR)标记对分别来自中国海南和云南 2 0个居群的疣粒野生稻 (Oryzagranulata (NeesetArn .exWatt.) )混合样品 ,以及海南 (M5 )和云南 (M2 7) 2个居群各 2 0个植株的遗传多样性进行了检测。在混合取样的居群中 ,2 0个RAPD引物和 12个ISSR引物分别扩增出 2 0 9和 12 2条带 ,多态条带比率 (PPB)分别为 6 4.11%和 72 .95 %。对于分单株取样的居群 ,在M5中 ,两种标记分别扩增出 146和 95条带 ,PPB值分别为 2 1.48%和 34 .78%。在居群M2 7中 ,则分别扩增出 15 1(PPB =17.2 2 % )和 94(PPB =19.15 % )条带。结果表明 ,中国的疣粒野生稻在物种水平的遗传多样性较低 ,其遗传变异主要存在于海南和云南两地之间 ,而在居群内的遗传多样性水平很低。基于Jaccard遗传相似性系数的UPGMA聚类和基于多态条带表型的主成分分析 (PCA)都将中国的疣粒野生稻分为来自海南和云南两类 ,两地间该物种的平均遗传相似性分别为 0 .5 96(RAPD)和 0 .5 11(ISSR) ,其间产生了相当程度的遗传分化。对比RAPD和ISSR在PCR反应中的稳定性和检测变异的能力表明 ,对于实验条件的稳定性而言ISSR优于RAPD ,且总的来说ISSR能检测到比RAPD更多的遗传变异。Mantel检测表明 ,在物种水平 ,这两种标记

Fingerprints of broiler chickens divergently selected for very low density lipoprotein (VLDL) in fat line (FL) and lean line (LL) of the third generation were studied using inter simple sequence repeated (ISSR) technique Genetic polymorphism and genetic similarity coefficient between two groups were analyzed Ten primers were screened from 60 SSR primers and 62 sites were deprived Of which 39 bands were polymorphic sites and the frequency was 63 percent The genetic similarity coefficient among the individuals...

Fingerprints of broiler chickens divergently selected for very low density lipoprotein (VLDL) in fat line (FL) and lean line (LL) of the third generation were studied using inter simple sequence repeated (ISSR) technique Genetic polymorphism and genetic similarity coefficient between two groups were analyzed Ten primers were screened from 60 SSR primers and 62 sites were deprived Of which 39 bands were polymorphic sites and the frequency was 63 percent The genetic similarity coefficient among the individuals in FL and LL were 0 9643 and 0 9766 The genetic similarity coefficient between the FL and LL was 0 9481

通过对初步进行血浆极低密度脂蛋白 (VLDL)双向选择的三世代高、低脂群肉鸡进行ISSR指纹分析 ,评定其遗传多态性及两群体间的遗传相似系数。 6 0个引物筛选出 10个引物用于检测分析 ;共扩增出6 2条谱带 ,其中 39个是多态性片段 ,多态率为 6 3 %。高、低脂群群体内个体间遗传相似系数分别为 0 96 43 ,0 976 6。两群体间的遗传相似系数为 0 9481。

Simple sequence repeats (SSR) and Inter-simple sequence repeats (ISSR) are two kinds of DNA markers based on the polymerase chain reaction (PCR). They have been applied in many aspects of genome research. This paper has clarified the theories and characteristics of SSR as well as ISSR. The authers also summarized their applications in genetic polymorphisim and genetic relationship, establishment of DNA fingerprinting pool, construction of genetic map, gene localization and marker-aided selection. The...

Simple sequence repeats (SSR) and Inter-simple sequence repeats (ISSR) are two kinds of DNA markers based on the polymerase chain reaction (PCR). They have been applied in many aspects of genome research. This paper has clarified the theories and characteristics of SSR as well as ISSR. The authers also summarized their applications in genetic polymorphisim and genetic relationship, establishment of DNA fingerprinting pool, construction of genetic map, gene localization and marker-aided selection. The two methods of DNA ecular marker open up broad prospects for the studies of plant genetics and breeding.

SSR(SimpleSequenceRepeat)和ISSR (Inter-SimpleSequenceRepeat)技术是在PCR基础上发展起来的两种DNA多态性检测技术 ,已开始应用于基因组研究的各个领域。概述了SSR、ISSR反应的原理、特点 ,总结了其在植物亲缘关系和遗传多态性研究、DNA指纹库的建立、遗传图谱的构建和基因定位及分子标记辅助育种等方面的应用 ,并肯定了SSR、ISSR在植物遗传育种领域的广阔应用前景

 
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