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   5 '-primer 的翻译结果: 查询用时:0.012秒
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-primer
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  5’-启动子
     The 5'-primer Hind Ⅲ polymorphism, exon 4 Val158Met polymorphism and exon 6 BglⅠpolymorphism in COMT gene were selected as researched sites.
     选取位于儿茶酚氧位甲基转移酶基因启动子区域的5’-启动子HindⅢ多态、外显子4的Val158Met多态和外显子6的BglⅠ多态作为研究的位点。
短句来源
     They were all conducted the cognitive functional examination, and involved in the result analysis. ① Genotyping of three single nucleotide polymorphisms in COMT gene: The 5'-primer Hind Ⅲ polymorphism and exon 4 Val158Met polymorphism showed A/G polymorphism, while the exon 6 BglⅠpolymorphism showed C/- polymorphism.
     ①儿茶酚氧位甲基转移酶基因3个单核苷酸位点的基因分型:5’-启动子HindⅢ多态和外显子4Val158Met多态呈A/G多态、外显子6BglⅠ多态呈C/-多态;
短句来源
     ③ The exon 4 Val158Met polymorphism in COMT was associated with general quantity of words of oral fluency test, and the coefficient of product-moment was -0.19,P < 0.05. CONCLUSION: The 5'-primer Hind Ⅲ polymorphism and exon 6 BglⅠpolymorphism in COMT gene are not associated with schizophrenia, while the exon 4 Val158 Met polymorphism is related to the cognitive function of schizophrenia.
     ③患者组儿茶酚氧位甲基转移酶基因外显子4Val158Met多态与口语流畅性测验总词汇数相关,相关系数为-0.19,P<0.05。 结论:儿茶酚氧位甲基转移酶基因的5’-启动子HindⅢ多态、外显子6BglⅠ多态可能与精神分裂症无关联;
短句来源
  “5 '-primer”译为未确定词的双语例句
     Results:With wild-type MBL cDNA as template and Deep Vent DNA polymerase,the first round of PCR reaction was performed using 5'-primer and the internal mismatch primer,which destroys a Ban I site in code 54 of wild-type MBL gene,and a product of about 180 bp was obtained.
     结果 :以上游引物和诱变引物进行第一轮PCR ,获得一约 180bp的DNA片段 ,以此片段和下游引物行第二轮PCR扩增 ,得到一长约 780bp的产物 ;
短句来源
  相似匹配句对
     5) .
     <5>促进生殖发育。
短句来源
     5. P.E.
     5.当前高校体育课程资源没有得到合理的开发与利用。
短句来源
     0. 5 l of each primer ;
     上、下游引物各0.5川; dd HZO巧.
短句来源
     The sense primer sequence was 5'CAAGGTGACGCTGAATGG 3'.
     5,TeTGeee6eeeToe洲队T3’,下游引物序列为5’
短句来源
     The numberof bands are from 5 to 17 per primer.
     单个引物扩增片段标记数在5~17个之间。
短句来源
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  -primer
It also does not require 5'-primer extensions and finally delivers an expression clone for the preparation of untagged protein in less than a week.
      


The tetranucleoside triphosphate UpCpCpA has been synthesized by either the combined use of chemical and enzymic methods or chemical method alone. A schematic diagram is presented in Figure 1.The UpCpCpA is synthesized with PNPase which catalyzes the single addition of the 2'(3')-O-(α-methoxyethyl)-ADP to the primer UpCpC. The yield is 30%. The problems of the isolation of reaction produots and of the transnucleotidation are discussed.

用化学合成或化学与酶促合成相结合的方法均能制备得到UpCpCpA(图1)。化学合成包括“2+2”和“1+3”两条路线。参考Mackey和Gilham方法,在PNPase催化下,引物UpCpC与2′(3′)-O-甲氧乙基ADP反应进行单一加成可产生30%的UpCpCpA。为了获得纯的产品,采用了二次柱层析法(图2)。文中讨论了PNPase连接的转核苷酸作用问题。

This paper reports a new method of the phosphorylation of oligonucleotide at the 3'-end. Its principle is based on the polymerization of 7-methylguanosine 5' diphosphate (m~7GDP) on an oligonucleotide primer in the presence of polynucleotide phosphorylase (PNPase), followed by selective elimination of m~7G under mild conditions.Using two trinucleosides diphosphates CpCpA, CpUpC as primers, we have successfully synthesized two trinucleotides CpCpAp, CpUpCp.This method can also be applied to the ~(32)P...

This paper reports a new method of the phosphorylation of oligonucleotide at the 3'-end. Its principle is based on the polymerization of 7-methylguanosine 5' diphosphate (m~7GDP) on an oligonucleotide primer in the presence of polynucleotide phosphorylase (PNPase), followed by selective elimination of m~7G under mild conditions.Using two trinucleosides diphosphates CpCpA, CpUpC as primers, we have successfully synthesized two trinucleotides CpCpAp, CpUpCp.This method can also be applied to the ~(32)P labellation of polynucleotides at the 3'end.

本文报导了一种使寡聚核苷酸3'-端磷酰化的新方法。其原理是用多核苷酸磷酸化酶(PNPase),使7-甲基鸟苷5'二磷酸(m~7GDP)在引物存在下聚合,然后在温和的化学条件下,选择性消去7-甲基鸟苷(m~7G)。我们应用两种三核苷二磷酸CpCpA,CpUpC作引物,结果成功地合成了两种三核苷酸CpCpAp,CpUpCp。此方法还可用于多核苷酸的3'-~(32)P标记。

The synthesis of oligonucleotides by the PNPase catalyzed single-addition of the 2'(3')-o-(α-methoxylethyl)-ADP, UDP, GDP or ψDP on to trinucleoside diphosphate primer was described. When a reaction mixture containing CpUpG and ppU~(me) was incubated with PNPase for 7 hrs, the yield of the CpUpCpU~(me) was over 50%. When ppG~(me) was incubated instead with CpUpC under the same conditions, the yeild of the CpUpCpG~(me) was less than 10%. The yield of CpUpCpG~(me), however, could be raised to 50~70% when...

The synthesis of oligonucleotides by the PNPase catalyzed single-addition of the 2'(3')-o-(α-methoxylethyl)-ADP, UDP, GDP or ψDP on to trinucleoside diphosphate primer was described. When a reaction mixture containing CpUpG and ppU~(me) was incubated with PNPase for 7 hrs, the yield of the CpUpCpU~(me) was over 50%. When ppG~(me) was incubated instead with CpUpC under the same conditions, the yeild of the CpUpCpG~(me) was less than 10%. The yield of CpUpCpG~(me), however, could be raised to 50~70% when the reaction mixture was incubated at higher temperature, higher pH, higher substrate concentration.When GpCpm'I was incubated with ppψ~(me), no GpCpm'Ipψ~(me) could be detected. In the contrary, single-addition of ppψ~(me) to UpCpC allowed the production of UpCpCpψ~(me) in 40% yield. These results show that the single-addition synthesis of oligonucleotides catalyzed by PNPase is dependent on both the primer and the substrate. The significance of this specificity was discucced.

在PNPase利用2′(3′)-o-α甲氧乙基保护的NDP作为底物的单一加成反应中,CpUpC+ppU~(Me),37℃,7小时,CpUpCpU~(Me)的产率在50%以上,而CpUpC+ppG~(Me)在同一反应条件下,CpUpCpG~(Me)的产率仅10%以下。若提高反应温度、pH和底物的浓度等,可增加CpUpCpG~(Me)的产率至50~70%。GpCpm′I+ppψ~(Me)在上述反应条件下,没有获得所希望的反应产物(GpCpm′Ipψ~(Me)),而UpCpC+ppψ~(Me)时,则有UpCpCpψ~(Me)的生成。GpCpm′I+ppψ时,有少量GpCpm′Ipψ合成。这些结果说明,在PNPase的单一加成反应中,存在着底物和引物的特异性问题,因而对不同的底物或引物的最适反应条件是有差别的。

 
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