Monoclonal antibody (McAb) and polyclonal antibody to interleukin-2 receptor (IL-2R) and the second antibody labeled with horse radish peroxidase (HRP) were used to develop a high specific and sen-sitive sandwich type of enzyme-linked immunosorbent assay (ELISA) method to determine soluble IL-2R (sIL-2R).
Methods:The chlamydia trachomatis mixture of McAb5D9 and 1D6 was chosen as grasp antibody which was high specific and sensitive,PcAD was used as marked antibody and had been absorbed by chick embryo yolk sac.
Results NE was high specific for detecting viable myocardium and not different from that of DNE (85.5% vs 81.1%, P>0.05), but the sensitivity of NE was lower than that of DNE (44.9% vs 80.4%, P<0.05).
Conclusion: The method of the qualitative analysis of cTnI is simple and convenience, it has high specific and sensitive in diagnosis of AMI and it can be used on either ambulance or hospital.
Thernethod was simple,high specific and could be used accurately to number the OC cultivated in vitro,Thesubstrate being used was naphasol AS-BI which could be obtained easily,The OC cultivated on boneslices could survive as long as 240 hours.
Conclusions This was a simple,accurate,high specific method to detect CAs in the clinical samples and suggested to be a sensitive,reliable method for the dianosis of phechromocytoma.
Study on catalyst carrier nano-α-alumina with high specific surface area
A nano-alumina with high specific area was prepared using a homogeneous precipitation method with titanium dioxide and barium oxide as modifying additives.
The central point in this technique is the quantitative interpretation of data, especially, for substances with a high specific surface area.
Yakuts with a low body height, normotonic autonomic nervous regulation, a relatively high weight-height index, eukinetic blood circulation, and a high specific exercise performance are best adapted to the environment.
This method is applied at a high specific beam-energy flux (～1 kJ/cm2), one which causes noticeable erosion of the beam collector.
The author's previous work indicated that the activity of granular fused iron catalysts decreased seriously with time due to the gradual plugging up of the pore volume of catalysts by the high boiling point products. In this paper, the activity data were treated with Wheeler's internal diffusion law mathematically. And the results suggest that the rate is a chemical controlling one in the initial period of the synthesis, and the coefficient of the catalysts surface is of 0.98 (with space velocity 1000 hr~(-1),...
The author's previous work indicated that the activity of granular fused iron catalysts decreased seriously with time due to the gradual plugging up of the pore volume of catalysts by the high boiling point products. In this paper, the activity data were treated with Wheeler's internal diffusion law mathematically. And the results suggest that the rate is a chemical controlling one in the initial period of the synthesis, and the coefficient of the catalysts surface is of 0.98 (with space velocity 1000 hr~(-1), CO conversion 90％ or so). When the catalysts pore volume are plugged up by the high boiling point products (whether the pore to be micro or macro), the rate becomes an internal diffusion controlling one, and the effectiveness of the catalytic surface changed into 0.082. The calculated coefficient of diffusion is of 2.4 10~(-5) cm~2/sec, which is in agreement with the magnitude of liquid-phase diffusion. Under the diffusional controlling conditions, the activity level of catalysts depends upon Deff, ks and Sg, by the rate law k∝2~(1/Deff·ks·Sg.) Where change of effective diffusivity. Deff, under the liquid phase conditions is not essential. But both the specific reaction rate, ks, and the effective surface, Sg are the major factors to the catalytic activity improvements. Therefore the precipited iron catalysts, owing to its high specific surface area and high specific activity, may be the promising one of the granular catalysts suitable for the high space velocity synthesis.
The direct detection of plasma angiotensin Ⅱ (AT Ⅱ) by radioimmunoassay with 0.5ml of human blood was aohieved by utilizing fine-quality antisera, ~(125)I. AT Ⅱ of high specific activity and the double antisera separation technique.
The ~(14)C-labeled gossypol acetic acid (Sp.Act.2.665μci/mg) used in this experiment and the care and trea-tment of animals were the same asthe previous report.Wistar adult malerats selected for uniformity of weight(about 200g) were placed in individu-al metabolic cages,the feces,urine andexpired air were collected daily formeasurement in one group of animalsfollowing a single oral dose (20μci/7.5 mg) of ~(14)C-gossypol,acetie acid.The animals were anesthetized and kil-led after varying intervals of time (i.e.12...
The ~(14)C-labeled gossypol acetic acid (Sp.Act.2.665μci/mg) used in this experiment and the care and trea-tment of animals were the same asthe previous report.Wistar adult malerats selected for uniformity of weight(about 200g) were placed in individu-al metabolic cages,the feces,urine andexpired air were collected daily formeasurement in one group of animalsfollowing a single oral dose (20μci/7.5 mg) of ~(14)C-gossypol,acetie acid.The animals were anesthetized and kil-led after varying intervals of time (i.e.12 hours,1,2,4,9 and 14 days,3animals for each time).The bloodwas collected and various organ tis-sues as well as the contents of gastro-intestinal tract were removed,lyophili-zed and weighed ground for liquidscintillation counting by oxygen com-bustion.The free and bound gossypolwere extracted from parts of the aboveorgans,and an analysis of the ratioChanges of the free to bound gossypol(F/B) in the metabolic process wasmade.To the daily dose group,therats were given labeled gossypol atthe dosage of 4.5μci/5 mg daily and were anesthetized and killed 2 weeks,3 weeks,one and two weeks following the withdrawal of the drug (2 animalsfor each time period).Samples of tis-sues and excreta were prepared formeasurement according to the proce-dures as described above.The resultsobtained were summarized as follows.1.It was found that the injected~(14)C-labeled gossypol excreted rapidlythrough feces,urine and expired airfollowing the oral administration.Themain pathway of excretion was fromthe feces,followed by exhaled CO_2 andurine.The amount of ~(14)C eliminated in19 days was 83.50% in feces,11.73%in expired CO_2 and 2.51% in urine. The high excretion rate in feces sug-gested that the circulation and me-tabolism of gossypol through liver-bile-feces were the main pathway of elim-ination and detoxication of the gos-sypol from the body.The exhaledCO_2 appeared to be a metabolic pro-ducts of decarbonylation of formyl-~(14)C-gossypol,and thus,the decarbo-nylation is also one of the pathway ofelimination and dtoxication of gossypol.The low gossypol excretion in urine might be related to their nonionized large molecules of gossypol which were uneasied to pass through the renal glomeruli but were more favored toreabsorption by the renal tubules.2.After a single oral dose of~(14)C-labeled gossypol to male rats at thedosage of 2oμci/7.5mg/animal,thetime taken for the elimination fromthe body of one half of the radioac-tivity was 60 hours (i.e.t1/2=2.5days).According to this half-life valueof excretion,it was possible to cal-culate that the time for the clearanceof gossypol from the body would beat the 23rd day or nearly so,it wasmostly corresponding to our data thatit took 19 days to eliminate 97.74%of the dose from the animal body.3.The data obtained by radioac-tivity measurement of the tissues fromrats fed ~(14)C-labeled gossypol demon-strated that the contents and the epi-thelial lining of gastro-intestinal tractand the liver had the highest specificradioactivity.They reached their peaklevels one day following a single oral dosing.The blood also reached its peak at the first day,however,its specific activity was lower than that of the above visceral organs.The specific radioactivity in the heart,kidney,sple-en,lung,pancreas,diaphragm andtestis were low at the early stages ofdrug adiministration,but rapidly rea-ched to their peak at the 4—9th day.Among the organs,the spleen,kidneyand heart contained more higher speci-fic activity than that of other organs.The endocrine organs such as adrenal,pituitary and thyroid gland also con-tained a fairly high specific activity.Whereas,the hypothalamus,medullaoblongata and spinal cord had the lo-west activity only.The specific acti-vity in various organs decreased ingeneral with the increase of time dura-tion,and dropped to their minimumamount 19 days after the administra-tion.4.The pharmacokinetics of ~(14)C-gossypol in rats that on daily dosingwere almost the same as in the rats ofsingle oral dosing group.Two weeksfollowing the administration,all tis-sues contained radioactivity,with the brain having the lowest activity.The contents and tissues of alimentary canal as well as liver had the highest specific acitivity also,followed by spleen,kidney,heart,lung pancrease,diaphragm and testis.They reached their peak levels after 3 weeks of daily dosing.But thereafter,the specific acitivitysteadily decreased to a lower level.Two weeks after the withdrawal oftreatment,the activity in various tis-sues decreased to their minimum am-ounts.These reaults indicated thatthe pharmacokinetics of ~(14)C-gossypolin both dosage groups were basicallysimilar in distribution pattern,and thedata of quantitative measurement andautoradiographic investigation werecoincided with each other either.5.The results demonstrated thatthe ratios of free gossypol (F) to boundgossypol (B) in the contents and tissuesof different parts of the gastro-intes-tinal tract,liver,and feces were lowat the early stages of drug administr-ation.However,the value of theseratios had progressively increased withtime in both dosing grops.These data suggested that the gossypol might mainly metabolized as a binding form during early stages of medication within rat body.Subsequently,the proportion of free gossypol and other metabolites increased with time throughoxidation,hydrolysis and decarbonyla-tion of the bound gossypol in varioustissues,resulting in soluble or air formand cxcreted via feces,urine and expir-ed air.
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