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-proximal
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  5’端
     Effect of the 5'-proximal sequence of HCV 5'NCR on its translation initiation activity
     丙型肝炎病毒5’端非编码区的5’端序列对其翻译启动活性的影响
短句来源
     Methods The fragments of full-length HCV 5' noncoding region (5' NCR )and truncated HCV 5'NCR with deletion of the 5'-proximal 17 nucleotides were amplified with PCR, and were fused in-frame into immediate upstream of SEAP gene of pSEAP2-Control, a SEAP eukaryotic expression plasmid, to generate recombinant plasmids pNCRSEAP and pdNCRSEAP respectively, which were confirmed with enzymatic digestion and DNA sequencing.
     方法用PCR技术扩增获得全长和5’端17个碱基缺失的HCV5’NCR片段,定向克隆至表达质粒pSEAP2Control的SEAP基因上游,分别构建成全长和5’端截短的HCV5’NCR调控SEAP表达的重组质粒pNCRSEAP和pdNCRSEAP。
短句来源
     Conclusion Deletion of the 5'-proximal 17nucleotides of HCV 5'NCR can enhance its translation initiation activity for SEAP gene,and lay experimental foundation for further study on the structure of HCV IRES
     结论HCV5’NCR的5’端碱基缺失提高了它对SEAP基因的翻译启动活性,为进一步分析HCVIRES的结构提供了实验基础。
短句来源
  “5 '-proximal”译为未确定词的双语例句
     Sequence analysis revealed the occurrence of two overlapping open reading frames (ORFs): the 5' -proximal large ORF (ORF1; nucleotide positions 62-2389) encodes a putative coat protein (CP) with a predicted molecular mass of 80344 Da, and the 3'-proximal ORF (ORF2, nucleotide positions 2386-4875) encodes a putative RNA dependent RNA Polymerase (RdRp) with a predicted molecular mass of 82551 Da.
     ORF1位于该dsRNA的正链上62—2389nt,编码一个分子量为80344Da的外壳蛋白(Coat Protein, CP); ORF2位于正链上2386—4875nt,编码一个分子量为82511Da的依赖于RNA的RNA聚合酶(RdRp);
短句来源
  相似匹配句对
     5)
     (5)火烧迹地上的樺、柳林。
短句来源
     5M.
     9例健康头屑标本,分离到M.
短句来源
     5:l-1:l;
     0.5∶1~1∶1,填料具有较好的应用性。
短句来源
     (5)If R-(?)
     (5)如(?)
短句来源
     (6)proximal turbidite.
     (6)近源浊积灰岩.
短句来源
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  -proximal
The 5'-proximal ORF encodes a 27?kDa protein (p27) and terminates with an amber codon which may be read-through into an in-frame p56 ORF to generate a 86?kDa protein (p86) containing the viral RNA dependent-RNA polymerase motifs.
      
The nucleotide sequence identity within the 3'-untranslated region (3' UTR) was 84.7-100%, and the region was variable in length (303-308 nucleotides) due to some deletions within the 5'-proximal part of the 3' UTR.
      
The 5'-proximal ORF coded for a putative coat protein (CP) and the 3'-proximal ORF encoded putative RNA-dependent RNA polymerase (RdRp).
      
The 5'-proximal open reading frame (ORF) encodes a putative 23?kDa protein and a 82?kDa protein by reading-through of an amber termination codon.
      
RNA 3 of alfalfa mosaic virus (AMV) encodes the 5'-proximal movement protein (MP) gene and the 3'-proximal coat protein (CP) gene which is expressed from a subgenomic RNA.
      
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The present paper deals with 2 spp. of nematodes, Cooperia laterouniformis Chen and Cooperia erschovi sp. nov. found in the pancreas of cattle in Peking, Inner Mongolia, Tsinan, Canton, Kweilin, Liuchow, Kunming and Kweiyang. The percentage of infection so far observed is fairly low, being only about 4%-28%. However, as the pancreas is an important organ intimately related with the metabolic processes, its infecton with Cooperia, hitherto considered as a genus parasitic solely in the alimentary tract, will certainly...

The present paper deals with 2 spp. of nematodes, Cooperia laterouniformis Chen and Cooperia erschovi sp. nov. found in the pancreas of cattle in Peking, Inner Mongolia, Tsinan, Canton, Kweilin, Liuchow, Kunming and Kweiyang. The percentage of infection so far observed is fairly low, being only about 4%-28%. However, as the pancreas is an important organ intimately related with the metabolic processes, its infecton with Cooperia, hitherto considered as a genus parasitic solely in the alimentary tract, will certainly draw attention of the veterinarian practitioners.The diagnostic characteristics of the new species are as follows:Body filiform, yellowish when living. Cuticle with fine transverse striations and abovt 10 longitudinal ridges. Head expanded, crown-like, separated from the body by a transverse cuticular depression. Cervical and prebursal papillae absent.Male: Body 4.688-11.080 mm long and 0.112-0.179 mm in greatest diameter. Head 0.016-0.019mm in length and 0.038-0.042mm in greatest diameter. Anterior end to nerve ring 0.322-0.378 mm. Oesophagus 0.378-0.483 mm long and 0.038-0.042 mm in greatest diameter. Bursa well-developed with two large lateral and one small dorsal lobes. Latero-ventral and ventro-ventral rays quite slender, almost equal in size. The 3 lateral rays equally stout. Postero-lateral with its distal half curved abruptly toward the middle, making the whole ray >-shaped. External dorsal smallest, separated from the main stalk at the level of the bifucation of the dorsal ray. Dorsal ray bifucated shortly after its leaving from its origin into two branches side by side; at the posterior 1/3 of each branch there being a stout sub-branch, with 45°declination to the outer side. Spicules 2 stout, deep brown pieces of equal length and similar shape, with series of concentric striations, giving the appearance of annual rings, as found in the stem of a dicot plant. Each spicule with a prominant indentation on the outerside at about 2/5 from the distal end, a blunt outgrowth near the proximal end, and a button-like structure on the top. Length of spicule 0.249-0.420 mm and greatest width 0.044-0.057 mm. No gubernaculum. Genital cone well-developed and rather complicated in structure. Ventral piece of genital cone with a blunt rod-like process on either, a V-shaped process at the middle of the posterior edge and a pair of horn-like appendages. Dorsal piece of genital cone as large as the ventral but without any process.Female: Body 8.840-11.400 mm long and 0.083-0.168 mm in greatest diameter. Head 0.016-0.019 mm long and 0.035-0.044mm in greatest diameter. Anterior end to nerve ring 0.294-0.364 mm. Oesophagus 0.350-0.448 mm tong and 0.035-0.048 mm in greatest diameter. Vulva slit-shaped, 1.680-2.200 mm to posterior end, coverd on its anterior margin with a cuticular ligule-like process, 0.126-0.134 mm in length. Combined length of ovijectors including sphincters 0.168-0.420 mm. Anus to the end of the body 0.168-0.224 mm, long and pointed, slightly curved to the ventral side. Ova in uterus 0.067-0.073 mm long and 0.028-0.041 mm in diameter.

1、在我国北京、內蒙古、济南、广州、桂林、柳州、昆明、贵阳各地的黄牛胰臟中普遍找 到古柏氏属线虫,其感染率为3.12-27.27%,此为反刍兽胰臟中首次发现的寄生线虫。 2、胰臟中所发现的古柏氏属线虫共有两种:一为Cooperia laterouniformis Chen,一为叶氏古柏线虫新种(Cooperia erschovi sp.nov.);后者的特徵为生殖锥的构造复杂,交合刺上有年轮狀环纹,背辐肋在其主干伸出不远处即分为两枝。 3、古柏氏线虫的成虫如何进入胰臟,有待于进一步的实验证明。 4、胰臟中的寄生线虫对于宿主的危害性,是值得注意的。新的寄生部位的发现应能促使畜牧兽医工作者,除注意反刍兽的消化道病理变化外,还应当考虑其胰臟的病理变化。

A new species of nematode, Ostertagia (G.) skrjabini sp. nov., was found in the abomasum of the Chinese goat in Kweiyang and Tsinan. The specific diagnosis of the new species is as follows:Body filiform, yellowish when living. The cuticle of the body is marked with about 30 longitudinal ridges and without fine transverse striations. The cuticle of the anterior end is slightly dilated. Cervical and preburrsal papilla present.Male: Body 7.890-11.770 mm in length and 0.145-0.182 mm in the greatest diameter, and...

A new species of nematode, Ostertagia (G.) skrjabini sp. nov., was found in the abomasum of the Chinese goat in Kweiyang and Tsinan. The specific diagnosis of the new species is as follows:Body filiform, yellowish when living. The cuticle of the body is marked with about 30 longitudinal ridges and without fine transverse striations. The cuticle of the anterior end is slightly dilated. Cervical and preburrsal papilla present.Male: Body 7.890-11.770 mm in length and 0.145-0.182 mm in the greatest diameter, and has a width at the preburrsal between 0.175-0.218 mm. Head 0.023-0.026 mm in great diameter. Nerve ring is situated close to the cervical papilla. Oesophagus 0.656-0.680 mm long and 0.049-0.059 mm in the greatest diameter. Bursa well developed with two large lateral and one small slightly depressed dorsal lobes and covered with bosses of different size. Bursa 0.332 mm in length and 0.300 mm in expansion, Latero-ventral and ventro-ventral rays almost equal in size, being separated in the proximal end, then getting gradually close to each other in the distal end and slightly curved toward the ventral side. The 3 lateral rays equally stout. Anterio-lateral ray slightly curved toward the veutal side, while the medio- and posterio- lateral rays curve toward the dorsal side and extend to the margin of the bursa. External dorsal ray large and stout in base, becoming gradually slender in the distal end, and being curved toward the dorsal ray. Dorsal ray 0.195 mm in length and bifurcated in the distal third into two branches, each branch subdivided into external and internal sub-branches at. the end the external sub- branch with a small papilla like structure in the outside. Spicules 1 pair, brown-colored, equal in length and of similar shape, measuring 0.251-0.280 mm in length and 0.036-0.039 mm in the greatest width. They are bifurcated in the distal third into dorsal, latero-ventral and medio-vcntral branches. The dorsal branch plate-formed, strongly chitinized and as large as the greatest width of the spicule. The distal margin of the dorsal branch rolls up toward the dorsal side, looking like a hook in the lateral view and like a inverted comma (') in the dorsal view. The latero-ventral branch is the longest, slightly curved to the ventral side, and surrounded by a transparent menbrane-like structure. Medio-ventraJ branch shorter and more slender than the latero-ventral, and without membranous structure in the distal end. 'Gubernaculum spindle-shaped.Female: Body 11.785-14.640 mm in length and 0.138-0.175 mm in the greatest diameter. Anterior end to cervical papilla 0.315-0.381 mm. Vulva slit-shaped, 0.129-0.175 mm to posterior end. Combined length of ovijectors including sphincters 0.249-0.415 mm. Anus to the end of the body 0.129-0.175 mm, long and pointed, slightly curved to the ventral side. Ova in uterus 0.072-0.082 mm in length and 0.040-0.050 mm in diameter.Host: Goat (Capra hircus L.)Location: Abomasum.Locality: China: Kweiyang and Tsinan.Type specimens: Deposited in the Laboratory of Helminthology, Institute of Zoologg, Academia Sinica, Peking, China.

(一)本文报告了济南和贵阳两地山羊第四胃中发现的奥思特线虫属Ostertagia斯氏奥思特线虫Ostetagia(Grosspiculagia)skrjabini sp.nov.一新种的寄生线虫,并作了特征上的描述。 (二)本新种交合刺的中腹枝长于背枝;背枝具有独特的呈倒置逗点状向背侧捲曲的末端;侧腹枝末端伸入一淡黄色泡状薄膜,该膜膨大成球状。由于侧腹枝两侧的角化较 强,以至形成一平行于主枝的淡黄色纵带。 (三)本新种导刺带呈梭形,其近端的正中线上有一条近乎下陷的槽。 (四)本新种背肋于远端1/3处分为2枝,其分枝间的距离很大,各分枝的末端又依次向外侧分出一小的侧枝和一根小的乳头状突起。 (五)本新种与O.(G.)Orloff,1933亚属中的各种作了特征上的比较。

The right sciatic nerve of the adult albino rat was sectioned and its proximal and distalsegments was taken at intervals various from 1--60 days for the observations to be made. Corres-ponding regions of the left sciatic nerve were used for comparison. The specimens were preparedaccording to MacDonald's and Danielli's modification of Gomori's methods for acid and alkalinephosphatases respectively. One month after sectioning of the nerve, the activity of the acid phosphatase in the proximalsegments was...

The right sciatic nerve of the adult albino rat was sectioned and its proximal and distalsegments was taken at intervals various from 1--60 days for the observations to be made. Corres-ponding regions of the left sciatic nerve were used for comparison. The specimens were preparedaccording to MacDonald's and Danielli's modification of Gomori's methods for acid and alkalinephosphatases respectively. One month after sectioning of the nerve, the activity of the acid phosphatase in the proximalsegments was found to be intensified in the retraction balls, myelin sheaths, Schwann cells and reg-enerating fibers. In the distal segment, the enzyme exhibited at first an increase in the axonsand schwann cells. That of the former disappeared along with the degeneration of the nerve, whilethat of the latter, remained to be intense and resumed to the normal activity after the completion ofregeneration 2 months later. The alkaline phosphatase is normally weak in the axons and Schwann cells. The enzyme inthe proximal segment was intensified in an area about 2 mm from the wound and in the regenera-ting fibers. That of the distal segment was weakened following the degeneration of the nerve.

坐骨神经被切断后,酸性磷酸酶在轴索缩回球、髓鞘和 Schwann氏细胞等处增强;再生纤维的轴索和髓鞘也都含有较正常纤维为多的酸性磷酸酶,而硷性磷酸酶在溃变的神经纤维中减弱,再生纤维中增强。

 
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