To analyse hereditary features and and rules of the foreign gene in the trans-Hu-IFN-α-gene carp,PCR testing technology were employed to test the Hu-IFN-α gene’s genetic expression in its cross F1 and gynogenesis F1 generation.
Methods The expression of VEGF and and its receptor FLT 1, FLK 1 mRNA were analized by reverse transcription polymerase chain reaction (RT PCR) in the specimens from 47 patients with breast cancer and 11 patients with benign breast disease.
On the foundation of comparing the current integrated network management normalization,it summarizes the advantages of Web Services in resolving the problem in Navy network management,and it analyses the Web Services protocol architecture and and compares with DCOM and CORBA ,and deeply probe into the facing service and inter-operation characteristic of Web Services.
Objective To observe the appearances of rheumatoid arthritis(RA) , assess the role of MRI in diagnosis and and evaluate disease prognosis of rheumatoid arthritis.
The objects of this thesis is numberical simulation of reacting flows in plane turbulent jets and and the applications in reacting flows of selective non-catalytic NOx reduction.
the patient with hyperammonaermia were 32 case, among 50 patients,there were 30 patients with Hp-lgG(+),the positive rate of is 60%(30/50 ), and and 2 patients with Hp-lgG (-),the negative rate of is 4%(2/50 ).
Of the liver cirrhosis group,the serum PCⅢ for HBeAg positive subgroup and and that for anti HBe positive subgroup were 133.9±20.0 μg/L and 172.9±52.2 μg/L respectively, showing marked difference( P <0.05).
The local control rate,median survial period,one year survival rate and mortality were 88.2%,12.5 months,64.7% and 35.3% in SRT plus WBI group,respectivel,and and 90.5%,7.6 months,47.6%and 52.4% in SRT alone group(P>0.05),respectivel.
Nevertheless, Hg2+ can inhibit activity of aquaporins, and and LP of roots decreased 22% in distilled water and 68% in NH4NO3 solution after treatment by HgCl2 respectively.
Electrochemical biosensors based on nucleic acids and their use in bioaffinity assays for determining DNA and and its effectors
Quasiconcave functions and belong to the same scale if there exist quasiconcave functions and and numbers such that and .
Diagnostic testing of programmable logic arrays that directly implement Boolean functions specified in a polynomial form (i.e., as a combination of AND and EXOR arrays) is considered.
An equivalent electrical network of a DNA molecule is designed, which could serve as a building block of nanoscale digital logic such as the NOT, OR, and AND gates.
In our study on the synthesis of glucosaminides we have employed 2,4-dinitropheuyl group (DNP) to protect the amino group of glucosamine, and foundthat DNP substitution brings forth an anomalous change in the optical rotation. By following Kent's procedure we obtained from 2-amino-2-deoxy-D-glucose hydrochloride (Ⅰ) N-DNP-2-amino-2-deoxy-D-glucose (Ⅱ). Its meltingpoint, 194°(200--202°, Kofler block), agrees with that reported by Meyer et al.(196±2°) and Morgan et al. (202-4°), but differs from that given by...
In our study on the synthesis of glucosaminides we have employed 2,4-dinitropheuyl group (DNP) to protect the amino group of glucosamine, and foundthat DNP substitution brings forth an anomalous change in the optical rotation. By following Kent's procedure we obtained from 2-amino-2-deoxy-D-glucose hydrochloride (Ⅰ) N-DNP-2-amino-2-deoxy-D-glucose (Ⅱ). Its meltingpoint, 194°(200--202°, Kofler block), agrees with that reported by Meyer et al.(196±2°) and Morgan et al. (202-4°), but differs from that given by Kent(167-9°). The specific rotation values reported by all workers being practicallythe same([α]_D~(23)+50°; [α]_(5463)~(23)+68.7° in 80% ethanol). Acetylation of (Ⅱ) withacetic anhydride and pyridine gave a tetra-acetyl derivative (Ⅲ). Its meltingpoint (214-5°) and [α]_D~(20)(+12°, CHCl_3) also differed from those given by Kent(159-60°; [α]_D~(19)+73°, CHCl_3). (Ⅲ) was nearly quantitatively converted into1-bromo-3, 4, 6-tri-O-acetyl-N-DNP-2-amino-2-deoxy-α-D-glucose (Ⅴ) in usualway. Compound (Ⅴ) is identical with Stacey's bromo-compound (m. p. 162-4°[α]_D~(19)+46.0°, CHCl_3), obtained from another tetraacetyl-N-DNP-derivative (Ⅳ).Since (Ⅳ) was derived from the known 1, 3, 4, 6-tetra-O-acetyl-2-amino-2-deoxy-β-D-glucose (Ⅵ), and (Ⅲ) has also been prepared by us from 1, 3, 4, 6-tetra-O-acetyl-2-amino-2-deoxy-α-D-glucose hydrobromide (Ⅶ) of well establishedα-configuration, these two DNP-compounds (Ⅲ and Ⅳ) should form a pairof α- and β-anomers. Merely on the basis of Hudson's isorotation rule, it shouldbe predicted that (Ⅳ) wore tho α- and (Ⅲ) the β-anomer, which are contradictoryto their configurations as expected from the starting materials used. In order toconfirm the configuration of the C_(1)-atom of the compounds in question, otherpairs of DNP-derivatives of glncosamine, such as (Ⅷ) and (Ⅸ), (Ⅹ) and (Ⅺ),together with (Ⅻ) and (ⅩⅢ) were prepared according to the methods sketchedin Fig. 1. Melting points and specific rotations of these products are presentedin Tablo Ⅰ. Since (Ⅸ) and (Ⅺ) possess higher values of [α]_D than the correspondinganomers (Ⅷ and Ⅹ), (Ⅸ) and (Ⅺ) would be assigned as α-glucosaminides and(Ⅷ) mid (Ⅹ) as the β-anomers, if Hudson's isorotation rule were also applicableto N-DNP-glucosamine derivatives as usual. However, after removal of DNP-groupwith barium hydroxide, (Ⅺ) yielded the known methyl-β-D-glucosaminide (ⅩⅢ)and (Ⅹ), the corresponding α-D-glucosaminide (Ⅻ) (Ⅻ). The configurationsof (Ⅹ) and (Ⅺ) have been further confirmed by direct syntheses of these anomersfrom tho known methyl N-acetyl-α-and β-D-glucosamindes (ⅩⅠⅤ and ⅩⅤ). Con-sequently, (Ⅷ) and (Ⅹ) must be α-type, and (Ⅸ) and (Ⅺ) β-type. The N- DNP-substitution causes an anomalous change in the rotatory power, so that thesubstituted β-glucosaminides are considerably more dextro-rotatory than the cor-responding α-anomers. From Table Ⅰ, it is clear that the differences between the molecular rotations[M]_D of the anomeric pairs (Ⅲ) and (Ⅳ), (Ⅷ) and (Ⅸ) its well as (Ⅹ) and(Ⅺ), (i. e. 2A values) are all negative in sign, while the 2A values for anomericD-glucosamines, D-glucosamine hydrochlorides, pentaacetyl-D-glucosamines andmethyl-D-glucosaminides hydrochlorides are all positive. The 2B values of N-DNP-substituted products have the same sign and nearly the same magnitudeas those of the ordinary anomeric glucosamine derivatives. Thus DNP groupat C_(2)NH_2 has greater influence on the [M]_D of C_(1)-atom than on that of theremaining part of the molecule. This vicinaI effect of DNP-substitution on [M]_Dof the C_(1)-atom may be (i) due to change in polarity , (ii) due to increasein the number of conjugated double bonds (or shift in the absorption bands ofthe substituent, or (iii) due to steric interaction between vicinal substituensof the molecule.
In this paper is presented a simplified two-variable approximate theory, based on the C. Libovc and S. B. Batdorf's theory[6] for elastic orthotropic plates with transverse shear deformations. Assuming that there exists a potential function (x, y) for the traps-verse shear angles rx and ry (see eq. (2.9)), the total potential energy If (eq. (2.8)) can be expressed in terms of two independent unknown functions, the plate deflection w(x, y) and the potential (x, y). By the use of the principle of minimum potential...
In this paper is presented a simplified two-variable approximate theory, based on the C. Libovc and S. B. Batdorf's theory[6] for elastic orthotropic plates with transverse shear deformations. Assuming that there exists a potential function (x, y) for the traps-verse shear angles rx and ry (see eq. (2.9)), the total potential energy If (eq. (2.8)) can be expressed in terms of two independent unknown functions, the plate deflection w(x, y) and the potential (x, y). By the use of the principle of minimum potential energy the Eulcr dcffercntial equations (1.11) for w and and the boundary conditions (1.12)-(1.15) are obtained in Appendix I. The comparision between the results for critical compressive load for a particular case of square simply-supported plate based on the present theory and Robinson's results[8] based on [6] shows that the discrepancy is small, if the anisotropy is not too significant (Table I). It is shown in Appendix H. that for polygonal simply-supported isotropic plates for both the bending and the stability problems the present theory always gives the same results as the theory in [6]. Two kinds of free edges arc distinguished: "entirely free edges" with the boundary conditions as (3.14) and the "stiffened free edges" with the boundary conditions as (3.17). Analysis of examples for orthotropic plates with free edges shows that, in general, cannot be interpreted as the shear deflection.
Summary This paper discusses the circuit synthesis and development technique of a transistor pulse synchronized frequency division system.It consists of many ele- mentary pulse networks such as:Transistor forming and shaping circuit,trrnsistor blocking oscillator,multivibrator,time delay circuit and “and” gate,etc.The master signal of this system is supplied from a 100—KC/S crystal controlled oscillator.With the use of many stages of both-side synchronized frequency di- viders,the system simultaneously delivers...
Summary This paper discusses the circuit synthesis and development technique of a transistor pulse synchronized frequency division system.It consists of many ele- mentary pulse networks such as:Transistor forming and shaping circuit,trrnsistor blocking oscillator,multivibrator,time delay circuit and “and” gate,etc.The master signal of this system is supplied from a 100—KC/S crystal controlled oscillator.With the use of many stages of both-side synchronized frequency di- viders,the system simultaneously delivers stable sharp pulses of 100—KC/S, 10—KC/S,1—KC/S and 100—C/S repetition frequency.The duration of each pulse of the lowest repetition frequency is about 0.4μs,and shorter for the high repetition frequencies.The amplitudes of the pulaes of the different repetition frequencies are more than 3 volts.The resulting phase shift and drift instability of the cascade synchronized frequency dividers are eliminated by pulse selection method in this system,Hence the output pulses are accurately positioned in time relative to each other.Test results coincide fairly well with theoretical analysis.
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