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copper binding
相关语句
  铜结合
     2. Purification copper binding protein using G - 25 column and copper chelating column.
     2.通过G-25层析和铜结合层析纯化大鼠肝脏溶酶体中与铜具有亲和性的蛋白质。
短句来源
     Isolation Two Copper Binding Proteins Related with Plant Resistance to Pathogens from Algae, Its Properties and the Characteristics of Gene
     海藻中两种与植物抗病相关铜结合蛋白的分离、特性和基因特征
短句来源
     Isolation and purification of a novel copper binding proteins in hepatic lysosome of rats
     大鼠肝脏溶酶体中新的铜结合蛋白的分离和纯化
短句来源
     1. We isolated . a novel copper binding protein ( 58 KD).
     1.经上述的处理和分析,大鼠肝脏溶酶体中铜结合蛋白质(分子量58KD)与哺乳动物蛋白质无同源性。
短句来源
     Objective: To isolate copper binding protein from rat liver lysosomal fraction, and to analyze its amino acid sequences.
     目的:分离大鼠肝脏溶酶体中铜结合蛋白质,并分析其氨基酸序列。
短句来源
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  “copper binding”译为未确定词的双语例句
     2. Kinetic process of copper binding to human serum albumin (HSA) was first studied on the MES modified gold electrode with stripping voltammetry.
     2.率先采用巯基乙磺酸钠(MES)修饰金电极结合溶出伏安法来研究铜与血清蛋白结合的动力学过程。
短句来源
     Results Analysis of copper -induced conformational changes of Atox1 indicated that both secondary and tertiary structure changes took place upon copper binding. These copper-induced conformational changes could play an important role in the function of Atox1 in vivo .
     结果 结合铜以后 ,Atox1无论是二级结构 ,还是三级结构都发生了变化 ,铜诱导的构象变化对于Atox1在体内发挥其作用时可能起关键作用。
短句来源
     Many studies indicate that although hemocyanins and phenoloxidases (PO) of mollusks and arthropods serve different physiological functions, but they both have copper binding sites, and Hc can be functionally converted into a kind of phenoloxidase-like enzyme (hemocyanin-derived phenoloxidase, HdPO) and display PO activities under certain conditions. Biochemically characterization of HdPO has not only great significance in understanding its roles in invertebrate non-specific immune system, but also helps us to fully cognize the multiple biological functions of Hc.
     很多研究表明,软体动物及节肢动物的Hc与酚氧化酶(phenoloxidase,PO)虽然功能迥异,但在结构上均具有Cu~(2+)结合位点,且在特定的条件下,Hc又能转化为酚氧化酶样酶(hemocyanin-derived phenoloxidase,HdPO)而表现出PO活性。
短句来源
     The two copper binding sites are about 30 A apart.
     两个相距约为30(?) 的铜(Ⅰ)、铜(Ⅱ)结合部位位于桶状结构的两端。
短句来源
     3. Analysis of copper binding protein by SDS - PAGE, three main protein bands observed. The main bands were digested by lysyl endopeptidase and isolate different peptides by HPLC.
     3.SDS-PAGE分析得到3条主要蛋白带,剪下这三条带进行胶内赖氨酸内切酶的消化,通过高效液相色谱分离肽段,选择性进行肽段的氨基酸序列测定。
短句来源
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  相似匹配句对
     COPPER
     铜
短句来源
     Study on Binding Eqilibrium of Gelatin with Copper
     明胶与Cu~(2+)的结合平衡研究
短句来源
     Study on Enhancement of Binding Force on the Interface of Coating on Copper
     增强铜表面热喷涂涂层结合力的探讨
短句来源
     COPPER IN STEELMAKING
     炼钢过程中的铜
短句来源
     protein binding;
     蛋白结合;
短句来源
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  copper binding
Indeed, S100A13 is a copper binding protein able to enhance the export of FGF1 in response to stress in vitro and to induce the formation of a multiprotein aggregate responsible for FGF1 release.
      
In addition, we speculate on how copper binding may regulate the activity and intracellular distribution of WNDP, and what role the human copper chaperone Atox1 may play in these processes.
      
Structural features include a distorted tetrahedral copper binding site at one end of an eight-stranded antiparallel β-barrel, a pronounced negative patch, and a flat hydrophobic surface.
      
After five cycles, copper binding actually increased slightly, probably due to enhanced exposure of binding sites in the walls.
      
Repeated use of Bacillus subtilis cell walls for copper binding
      
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Hepatolenticular degeneration (HLD) is an autosomal recessively inherited disease caused by copper metabolic dysfunction. Its primary genetic defect is still not known. A previous study showed that the average copper content of HLD cells was approximately threefold that of normal cells. Cultured fibroblasts were used as an in vitro model to investigate primary molecular defects. We examined the distribution of protein-bound intracellular copper in proteins of HLD cells. Cell lysates were fractionated by gel...

Hepatolenticular degeneration (HLD) is an autosomal recessively inherited disease caused by copper metabolic dysfunction. Its primary genetic defect is still not known. A previous study showed that the average copper content of HLD cells was approximately threefold that of normal cells. Cultured fibroblasts were used as an in vitro model to investigate primary molecular defects. We examined the distribution of protein-bound intracellular copper in proteins of HLD cells. Cell lysates were fractionated by gel fitration. Copper concentration of columnar fractions were determined by anodic stripping voltammetry. Results revealed that the character of copper binding was altered in HLD cells.A decreased ratio of copper to proteins was observed in cytoplasmic proteins having a molecular weight≥30 000. There was more copper specifically bound to lower molecular weight compounds in HLD cells, these results also may serve as a method for early unequivocal diagnosis of this di sorder.

在已经建立的肝豆状核变性(HLD)细胞模型基础上,用凝胶层析法和阳极溶出伏安法,研究铜在皮肤成纤维细胞不同蛋白组分中分布。结果:HLD患者细胞中第一洗脱蛋白峰的铜/蛋白质比值大大低于对照,患者过量累积铜主要与小分子蛋白质结合。此法可用于临床对该病的早期诊断。

Ssveral experiments have been done to demonstrate molybdate sulphur and their compound-trithiomolybdate could affect the metabolism of copper bound proteins in liver cytosol, especially copper on the metallothioncin. A possibility is that molybdate strip copper from metallothionein in the cytosol of liver of cattle. Copper stripped either moved out of cells as a small molecullar compound in vivo, or combined to a large molecullar proteins in cytosol in vitro. Meanwhile, metallothionein...

Ssveral experiments have been done to demonstrate molybdate sulphur and their compound-trithiomolybdate could affect the metabolism of copper bound proteins in liver cytosol, especially copper on the metallothioncin. A possibility is that molybdate strip copper from metallothionein in the cytosol of liver of cattle. Copper stripped either moved out of cells as a small molecullar compound in vivo, or combined to a large molecullar proteins in cytosol in vitro. Meanwhile, metallothionein may capture copper from other source, for example, a large molecullar copper bound proteins. The peak of metallothionein appcars when thiomolybdate excreted from cells. Dithiothretol can stablize the combination of copper to metallothionein. There is no obvious effect on the combination of copper to the proteins when samples were stored in -20℃ for five weeks.

以8头公犊为材料,通过体内和体外试验证明硫、钼及其化合物三硫钼酸盐被吸收或直接注入血液进入肝脏后,对肝内铜代谢的干扰作用主要是促使铜与咪噻宁蛋白质分离。分离后的铜成为小分子化合物离开肝细胞,而在体外试验中,还有部分铜转移到分子量较大的蛋白质上。一旦硫钼酸盐从肝内排泄后,体内铜很快与咪噻宁重新结合。缓冲液中二硫苏糖醇可使铜与咪噻宁结合保持恒定,样品在冰冻情况下保存5周,对咪噻宁与铜结合作用无明显影响。

In the paper, the action of metal ions on the human serum albumin had been investigated by fluorescence quenching method. The results showed that Cu~(2+), Mn~(2+), Co~(2+), Cr~(3+),Eu~(3+),Ni~(2+) may bind to human serum albumin and that the fluorescence of human serum albumin may be quenched by nonradiative energy transfer. In terms of Forster type dipoledipole nonradiative energy transfer the distance between the Trp-214 and the bound Cu~(2+) in the first copper-binding site was calculated...

In the paper, the action of metal ions on the human serum albumin had been investigated by fluorescence quenching method. The results showed that Cu~(2+), Mn~(2+), Co~(2+), Cr~(3+),Eu~(3+),Ni~(2+) may bind to human serum albumin and that the fluorescence of human serum albumin may be quenched by nonradiative energy transfer. In terms of Forster type dipoledipole nonradiative energy transfer the distance between the Trp-214 and the bound Cu~(2+) in the first copper-binding site was calculated and found to be about 1.20nm.

通过金属离子对人血清白蛋白内源荧光的猝灭,探讨了金属离子Cu~(2+),Mn~(2+),Ni~(2+),Co~(2+),Cr~(3+)等与人血清白蛋白的结合;基于Forster无辐射能量转移机理,得出了人血清白蛋白第一类Cu~(2+)结合部位与214位色氨酸残基间的距离。

 
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