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   copper binding 在 生物学 分类中 的翻译结果: 查询用时:0.192秒
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copper binding
相关语句
  铜结合
    2. Purification copper binding protein using G - 25 column and copper chelating column.
    2.通过G-25层析和铜结合层析纯化大鼠肝脏溶酶体中与铜具有亲和性的蛋白质。
短句来源
    1. We isolated . a novel copper binding protein ( 58 KD).
    1.经上述的处理和分析,大鼠肝脏溶酶体中铜结合蛋白质(分子量58KD)与哺乳动物蛋白质无同源性。
短句来源
    Isolation of a Novel Copper Binding Protein from Rat Lysosomal Fraction
    大鼠肝脏溶酶体中新的铜结合蛋白的分离和纯化
短句来源
    Two putative copper binding sites are present and they have a highly conserved sequence around these sites.
    中国对虾酚氧化酶原(proPO)cDNA片断含有两个公认的铜结合位点,两个位点周围的组氨酸高度保守。
短句来源
    The protein contains an apoptosis inhibitory protein 5(API5) domain,an involucrin repeat,and a copper binding octapeptide repeat.Blast alignment against human genomic database revealed that the gene contains eight exons,and is assigned to human chromosome 2p22.3.A multiple tissue cDNA based RT-PCR showed that hPPB is ubiquitously expressed in human normal tissues and human tumor tissues.
    该cDNA编码的蛋白序列含有一个凋亡抑制蛋白5结构域,外皮蛋白重复片段和铜结合辛肽重复片段. RT-PCR分析显示该基因在人类正常组织和癌组织中广泛表达,但在癌组织中表达量相对较低,提示其可能对细胞凋亡有抑制作用.
短句来源
  “copper binding”译为未确定词的双语例句
    3. Analysis of copper binding protein by SDS - PAGE, three main protein bands observed. The main bands were digested by lysyl endopeptidase and isolate different peptides by HPLC.
    3.SDS-PAGE分析得到3条主要蛋白带,剪下这三条带进行胶内赖氨酸内切酶的消化,通过高效液相色谱分离肽段,选择性进行肽段的氨基酸序列测定。
短句来源
    2. We use the novel copper binding protein as antigen, prepared rabbit anti rat copper binding protein polyclonal antibody.
    2.免疫家免得到了兔抗大鼠钢结合蛋白质的多克隆抗体。 讨 论
短句来源
    Researches show that copper binding proteins have conserved amino acid sequences - MXCXXC ( X is any amino acid) , can bind with copper.
    Irene等人的研究表明钢结合蛋白具有高度同源性的钢结合氨基酸序列,从酵母等低等动物到真核细胞N末端都有MXCXXC X代表任意氨基酸厂是钢结合的区域,例如 AI”o B的 N末端就有 5个 MXC状C结构,实验也证明一摩尔的蛋白质可与5摩尔的硫酸铜结合。
短句来源
    Many studies indicate that although hemocyanins and phenoloxidases (PO) of mollusks and arthropods serve different physiological functions, but they both have copper binding sites, and Hc can be functionally converted into a kind of phenoloxidase-like enzyme (hemocyanin-derived phenoloxidase, HdPO) and display PO activities under certain conditions. Biochemically characterization of HdPO has not only great significance in understanding its roles in invertebrate non-specific immune system, but also helps us to fully cognize the multiple biological functions of Hc.
    很多研究表明,软体动物及节肢动物的Hc与酚氧化酶(phenoloxidase,PO)虽然功能迥异,但在结构上均具有Cu~(2+)结合位点,且在特定的条件下,Hc又能转化为酚氧化酶样酶(hemocyanin-derived phenoloxidase,HdPO)而表现出PO活性。
短句来源
    The two copper binding sites are about 30 A apart.
    两个相距约为30(?) 的铜(Ⅰ)、铜(Ⅱ)结合部位位于桶状结构的两端。
短句来源
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  copper binding
Indeed, S100A13 is a copper binding protein able to enhance the export of FGF1 in response to stress in vitro and to induce the formation of a multiprotein aggregate responsible for FGF1 release.
      
In addition, we speculate on how copper binding may regulate the activity and intracellular distribution of WNDP, and what role the human copper chaperone Atox1 may play in these processes.
      
Structural features include a distorted tetrahedral copper binding site at one end of an eight-stranded antiparallel β-barrel, a pronounced negative patch, and a flat hydrophobic surface.
      
After five cycles, copper binding actually increased slightly, probably due to enhanced exposure of binding sites in the walls.
      
Repeated use of Bacillus subtilis cell walls for copper binding
      
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In the paper, the action of metal ions on the human serum albumin had been investigated by fluorescence quenching method. The results showed that Cu~(2+), Mn~(2+), Co~(2+), Cr~(3+),Eu~(3+),Ni~(2+) may bind to human serum albumin and that the fluorescence of human serum albumin may be quenched by nonradiative energy transfer. In terms of Forster type dipoledipole nonradiative energy transfer the distance between the Trp-214 and the bound Cu~(2+) in the first copper-binding site was calculated...

In the paper, the action of metal ions on the human serum albumin had been investigated by fluorescence quenching method. The results showed that Cu~(2+), Mn~(2+), Co~(2+), Cr~(3+),Eu~(3+),Ni~(2+) may bind to human serum albumin and that the fluorescence of human serum albumin may be quenched by nonradiative energy transfer. In terms of Forster type dipoledipole nonradiative energy transfer the distance between the Trp-214 and the bound Cu~(2+) in the first copper-binding site was calculated and found to be about 1.20nm.

通过金属离子对人血清白蛋白内源荧光的猝灭,探讨了金属离子Cu~(2+),Mn~(2+),Ni~(2+),Co~(2+),Cr~(3+)等与人血清白蛋白的结合;基于Forster无辐射能量转移机理,得出了人血清白蛋白第一类Cu~(2+)结合部位与214位色氨酸残基间的距离。

High quality RNA were isolated from hemocytes of Penaeus semisulcatus and P.monodon by SV total RNA isolation system.Specific primers for PCR were designed and synthesized according to the sequence of prophenoloxidase (proPO) of Penaeus monodon reported by Sritunyalucksana et al (1999) (GenBank Accession No.AF099741).Using the total RNA as templates,specific DNA fragments for both shrimps were obtained by reverse transcription PCR (RT- PCR).The DNA fragments were purified and cloned into T vector of pGEM-T...

High quality RNA were isolated from hemocytes of Penaeus semisulcatus and P.monodon by SV total RNA isolation system.Specific primers for PCR were designed and synthesized according to the sequence of prophenoloxidase (proPO) of Penaeus monodon reported by Sritunyalucksana et al (1999) (GenBank Accession No.AF099741).Using the total RNA as templates,specific DNA fragments for both shrimps were obtained by reverse transcription PCR (RT- PCR).The DNA fragments were purified and cloned into T vector of pGEM-T Easy Vector system.Recombinants were selected and confirmed by restriction enzyme analysis.Nucleotide sequences analyses of the recombinants show that the cloned cDNA of prophenoloxidase of P.semisulcatus contains the open reading frame 2055bp,from initiation codon ATG to termination codon TGA,which encodes 684 amino acids.While the cloned cDNA from P.monodon includes the open reading frame of 2061bp which encodes 686 amino acids.The molecular masses of the deduced amino acids sequences for P.semisulcatus and P.monodon are 78153Da and 78581Da,and their estimated pI are 6.25 and 5.83 respectively.Alignment analyses show that the nucleotide sequence and deduced amino acid sequence of prophenoloxidase of P.semisulcatus has high similarity to that of P.monodon (93% and 92% respectively).Both the nucleotide sequences and deduced amino acid sequences of prophenoloxidase of P.monodon cloned are highly similar to that of P.monodon,AF099741 (above 99%).And there are only 4 different nucleic acids in the 2kb sequence between the two sequences,which existing in the non-structural regions,so it is supposed that such differences would not influence the gene function.Homology of prophenoloxidase among shrimps and other arthropods are connected with their relative relationship.The prophenoloxidases of the compared species can be divided into three main groups.Three shrimps (P.monodon,P.semisulcatus and P.leniusculus) and one prawn (Macrobrachium japonicus) can be classified into the same subgroup.The two cloned prophenoloxidases,as well as that of the other arthropods species,contain two highly conserved putative copper binding sites and sequence around these sites.Such sites combining with two copper atoms would be part of the activity center of the enzyme.

采用RT PCR原理和长片段扩增技术克隆短沟对虾和斑节对虾酚氧化酶原基因。设计合成特异引物、提取血细胞总RNA ,反转录后扩增获得的特异片段回收并克隆到pGEM TEasyVector系统的T载体上 ,重组子进行碱基序列测定。结果表明 ,所克隆的短沟对虾酚氧化酶原基因含起始密码子ATG到终止密码子TGA的 2 0 5 5bp ,编码 6 84个氨基酸。斑节对虾酚氧化酶原基因含ATG到TGA的 2 0 6 1bp ,编码 6 86个氨基酸。短沟对虾酚氧化酶原推算分子量为 781 5 3Da ,等电点pI为 6 2 5 ;斑节对虾则为 785 81Da ,pI为 5 83。用DNA分析软件分析显示两种对虾酚氧化酶原基因具有高度的同源性 ,二者的碱基和推测的氨基酸序列同源性分别为 93 %和 92 %;对虾与其他节肢动物酚氧化酶原基因的同源性的高低与种类间亲缘关系相关 ;不同种类间酚氧化酶活性中心重要组成部分的 2个铜结合位点、位于铜结合位点内的6个组氨酸以及与铜结合位点相邻的氨基酸序列高度保守。

In a suppression subtractive hybridization experiment,attempted to reveal the character differences of adhesion and apoptosis between AD293 and HEK293,a cDNA fragment was isolated from an AD293 high expression library,which encodes a putative human phosphopantetheine binding protein(hPPB) inferred by electronic annotation.The 2745bp full-length cDNA was isolated from human fetal brain library,which encodes a protein of 518 amino acids.The protein contains an apoptosis inhibitory protein 5(API5) domain,an involucrin...

In a suppression subtractive hybridization experiment,attempted to reveal the character differences of adhesion and apoptosis between AD293 and HEK293,a cDNA fragment was isolated from an AD293 high expression library,which encodes a putative human phosphopantetheine binding protein(hPPB) inferred by electronic annotation.The 2745bp full-length cDNA was isolated from human fetal brain library,which encodes a protein of 518 amino acids.The protein contains an apoptosis inhibitory protein 5(API5) domain,an involucrin repeat,and a copper binding octapeptide repeat.Blast alignment against human genomic database revealed that the gene contains eight exons,and is assigned to human chromosome 2p22.3.A multiple tissue cDNA based RT-PCR showed that hPPB is ubiquitously expressed in human normal tissues and human tumor tissues.The transcript of the PPB gene is highly conserved during evolution.

在对AD293和HEK293进行差减杂交以探索两者在吸附和凋亡特性上的差异时,从AD293的高表达文库中分离得到一段新的cDNA片段.从人类胎脑文库克隆得到该基因,全长2 745 bp,编码的蛋白含518个氨基酸,被预测为磷酸泛酰巯基乙胺结合蛋白.该基因在染色体上定位于2p22.3,包含8个外显子.该cDNA编码的蛋白序列含有一个凋亡抑制蛋白5结构域,外皮蛋白重复片段和铜结合辛肽重复片段.RT-PCR分析显示该基因在人类正常组织和癌组织中广泛表达,但在癌组织中表达量相对较低,提示其可能对细胞凋亡有抑制作用.该基因在进化过程中高度保守.

 
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