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plaque-forming units
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  空斑形成单位
     Results: AdmIL-12 and Ad-EGFP were obtained, and their plaque-forming units were 1×10~8/mL and 1×10~9/mL.
     结果:得到AdvmIL-12和Adv-EGFP,每毫升空斑形成单位(PFU/mL)分别为1×108、1×109。
短句来源
  空斑形成单位(
     Results: AdmIL-12 and Ad-EGFP were obtained, and their plaque-forming units were 1×10~8/mL and 1×10~9/mL.
     结果:得到AdvmIL-12和Adv-EGFP,每毫升空斑形成单位(PFU/mL)分别为1×108、1×109。
短句来源
  “plaque-forming units”译为未确定词的双语例句
     Methods Keratitis was induced in BALB/c mice by inoculating the cornea with 10 5 plaque-forming units (pfu) of HSV-1 (KOS strain).
     方法 BALB/c小鼠眼角膜接种HSV 1(KOS株 )以诱发单纯疱疹病毒性角膜炎 (HSK)。
短句来源
     Viral transfection with 108 plaque-forming units was performed 2 days before the dorsal flap elevation.
     于大鼠背部正中设计蒂在尾侧的随意皮瓣,皮瓣的长宽为8cm×2cm,蒂部位于髂嵴水平。
短句来源
     Methods In a cervical heterotopic cardiac transplantation model by cuff technique, after harvest, guinea pig donor hearts’coronary arteries were perfused ex vivo with Stanford University solution containing 5×1010 plaque-forming units/g of donor heart of Ad. TGF-β_1, then implanted in the necks of complement depleted and immunosuppressed rat recipients.
     方法建立豚鼠到SD大鼠的颈部心脏移植模型,移植前受鼠接受中华眼镜蛇毒因子和环孢素A预处理,供心切取后,经冠状动脉按每克心肌组织灌注5×1010PFU携带TGF-β1基因的重组腺病毒载体进行基因转染,再移植到经过预处理的SD大鼠颈部(基因转染组),另设灌注5×1010PFU腺病毒空白载体的空白载体组和对照组。
短句来源
     Viral transfection with 10~8 plaque-forming units was performed 2 days before the dorsal flap elevation. Rats received subdermal injections of adenovirus encoding VEGF (Ad-VEGF) or green fluorescent protein (Ad-GFP) as treatment control. Another set of animals ( n =10) received PBS was designated as control.
     于皮瓣远端真皮下分别注射Ad-VEGF165cDNA(10只目的基因组)、Ad-GFP(10只绿色荧光蛋白基因对照组)、PBS(10只磷酸盐缓冲液空白对照组)。
短句来源
     HSV chorioretinitis model was induced in each mouse by inoculating 1×105 plaque-forming units (pfu) of HSV-Ⅰ (KOS strain)into anterior chamber of the right eye.
     将单纯疱疹病毒-型(HSV-)注入所有小鼠右眼(注射眼)前房内,建立单疱病毒性脉络膜视网膜炎的小鼠模型。
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  相似匹配句对
     Plaque determination
     蚀斑测定
短句来源
     MRI of atherosclerotic plaque
     动脉粥样硬化斑块的磁共振成像
短句来源
     group III-plaque group.
     SA组与对照组相比其斑块的发生率也有显著性差异(P<0.05);
短句来源
     Microstructure of Injection-Molded Polypropylene Plaque
     聚丙烯注射平板的微结构
短句来源
     Cutting and Forming
     切削加工与塑性加工 两大加工方法的技术革新与市场变化
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  plaque-forming units
Using this technique, AINO and AKA viruses were detected at concentrations as low as 10-3 plaque-forming units (PFU) and 10-5 PFU, respectively, in a supernatant of virus-infected cells.
      
Human neuroblastoma (IMR-32) cells were infected with herpes simplex virus type 2 (HSV-2) at a multiplicity of infection (MOI) of 2 plaque-forming units (PFU)/cell and were cultured at 40° C for 14 days.
      
Sparrows free of antibodies were inoculated with 10,000 plaque-forming units of JEV.
      
A mean of more than 1,000 plaque-forming units (PFU) of FMD virus per cell was obtained from cultures prepared as described for replication and assay of these strains of virus on second passage in cultured cells.
      
At multiplicities of exposure (plaque-forming units/ cell) less than 10, CPE was negligible in the poliovirus infected transformed cell cultures which could be maintained as living cultures yielding poliovirus up to three months.
      
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More than twenty isolates of the bacteriophage of Xanthomonas ory-zae from leaves and seeds of diseased rice plants and irrigation waterof diseased paddy fields were separated into two groups on basis of th-eir morphology and other properties.Phages of group XOP 1(repres-ented by isolate OP-3)produce large plaque about 3 mm in diame-ter.It is tadpole shaped with a polyhedral head of 65 nm in diameter,and a striated non-contractile tail of 140×18 nm in size.The latent pe-riod and raising period are respectively...

More than twenty isolates of the bacteriophage of Xanthomonas ory-zae from leaves and seeds of diseased rice plants and irrigation waterof diseased paddy fields were separated into two groups on basis of th-eir morphology and other properties.Phages of group XOP 1(repres-ented by isolate OP-3)produce large plaque about 3 mm in diame-ter.It is tadpole shaped with a polyhedral head of 65 nm in diameter,and a striated non-contractile tail of 140×18 nm in size.The latent pe-riod and raising period are respectively 40 and 30 minutes,and th-eir average burst size is about 35 plaque forming units(PFU)per cell.Phages of group XOP 1 are completely inactivated by heat treatment at58℃ for 10 minutes,and the unadsorbed free phages of this group areinactivated by ferric salts solution(10~(-4)M).Phages of group XOP 2,represerted by isolate OP-12,produce small plaque about 1 mm in dia-meter.It is also tadpole shaped,with polyhedral head of 70 nm in di-ameter,and a short contractile tail 90×20nm in size.The latent periodand raising period are 80 and 50 minutes respectively,and their averageburst size is about 8 plaque forming units per cell.Phages of groupXOP 2 are conpletly inactivated by heat treatment at 68℃ for 10 minutes.Serologically they are also different,experemented phages of groupXOP 1 and XOP 2 are specific to Xanthomonas oryzae,but phages of gro-up 2 is much broader in spectrum of its host than group XOP 1 judgingfrom their reaction to different isolates of Xanthomonas oryzae.

从感染白叶枯病的稻叶、种子和田水中先后分离到24株噬菌体,测定了它们在琼胶平板上形成噬菌斑的大小、血清学反应、热钝化温度、铁盐的钝化作用、寄主范围、一级生长曲线以及在电镜下观察它们的形态。根据测定结果,可将它们区分为两个类群。第一群称为XOP 1型,以OP—3为代表,其噬菌斑直径为3~5毫米,热钝化温度为58℃/10分钟,易为三价铁盐所钝化;寄主范围较窄;潜育期为40分钟,上升期30分钟,平均裂解量为35个。第二群称为XOP 2型,以OP—12为代表,噬菌斑小,热钝化温度68℃/10分钟,对三价铁盐不敏感,潜育期80分钟,上升期50分钟,平均裂解量为8个。它们都是蝌蚪形,但OP—3的尾部细长而不能收缩,OP—12的尾部短粗而能收缩。

This papei reports the effects of aciaci-nomycin B(ACM B) on multiplication and DNA polymerase activities of HSV-2. The experiment was carried out on Vero cell monolayer cultures and the plaque reduction assay was used as a main method evaluating the results. In the presence of ACM B, the plaque forming units of the virus was remarkably reduced.The plaque number was reduced to zero when the drug dose was beyond the level of 0.3 μM.The results showed that ACM B inhibited HSV-2 multiplication. Besides, ACM...

This papei reports the effects of aciaci-nomycin B(ACM B) on multiplication and DNA polymerase activities of HSV-2. The experiment was carried out on Vero cell monolayer cultures and the plaque reduction assay was used as a main method evaluating the results. In the presence of ACM B, the plaque forming units of the virus was remarkably reduced.The plaque number was reduced to zero when the drug dose was beyond the level of 0.3 μM.The results showed that ACM B inhibited HSV-2 multiplication. Besides, ACM B also inhibited HSV-2 induced DNA poiymerase. It was presumed that the inhibitory action of ACM B to HSV

本文报道了阿克拉霉素B(ACM-B)对单纯疱疹病毒Ⅱ型(HSV-2)复制及其DNA聚合酶活性的影响。实验采用单层Vero细胞,空斑降低测定用来评价实验结果。加入ACM-B,明显地降低病毒空斑的形成。当ACM-B剂量大于0.3μm时,空斑数降低到零,表明ACM-B抑制HSV-2的复制。另外,ACM-B也抑制HSV-2诱导的DNA聚合酶活性。这提示,药物对HSV-2复制的抑制作用可能是由于对病毒DNA合成的抑制。吸收光谱分析证实,ACM-B能与活化DNA模板相互作用。ACM-B也抑制E.Coli DNA pol.Ⅰ及L1210DNA pol.α,证明ACM-B对HSV-2 DNApol.的抑制是非选择性的.ACM-B对HSV-2复制的抑制效应较ACM-A强,而细胞毒性效应也较后者为高。

Poriatin is an physiologically active component of Poria,a traditional Chinese medicine, and consists of a group of homologs with low molecular weight. When mice were administered once intraperitoneally with different dosages of Poriatin, both cell number and morphology of peritoneal macrophages changed significantly 4 days later, lysosomal enzyme content and release and pinocytic and phagocytic rate being increased concomitantly with increment of biosynthesis of protein and RNA. Por-iatin-elicited macrophages...

Poriatin is an physiologically active component of Poria,a traditional Chinese medicine, and consists of a group of homologs with low molecular weight. When mice were administered once intraperitoneally with different dosages of Poriatin, both cell number and morphology of peritoneal macrophages changed significantly 4 days later, lysosomal enzyme content and release and pinocytic and phagocytic rate being increased concomitantly with increment of biosynthesis of protein and RNA. Por-iatin-elicited macrophages also showed changes in ectoenzyme activities and inhibition of cytopathic effect (CPE) and plaque-forming unit (PFU) induced by herpes simplex virus type 1 (HSV-l) on Vero cells. The above results indicate that Poriatin could activate mouse peritoneal macrophages in vivo.

给小鼠腹腔1次注射不同剂量的茯苓素后,小鼠腹腔巨噬细胞百分数、形态以及细胞膜酶活性发生明显改变,吞饮、吞噬功能和溶酶体酶含量及释放显著提高,并伴随RNA和蛋白质合成加快。茯苓素体内诱导的小鼠腹腔巨噬细胞在体外抗病毒作用加强.

 
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